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Servicebio Inc bcip nbt kit
Bcip Nbt Kit, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bcip/3+3%E2%80%99+dab+diaminobenzidine/pm42287828-87-17-21
Average 86 stars, based on 1 article reviews

bcip nbt kit - by Bioz Stars, 2026-11

86/100 stars

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Saline:

Article Title: MT2A-Mediated Regulation of Cuproptosis in Human Dental Pulp Cells Modulates Macrophage M1 Polarization in Pulpitis.
Article Snippet: The sections were incubated with lip-DLAT (Abcam, UK) antibodies overnight at 4°C. .. After washing the sections with phosphate-buffered saline (PBS) to remove any primary antibody, the sections were then incubated with a secondary antibody (Proteintech, Wuhan, China) at room temperature for 1 h. Signals were detected using an enhanced 3,30-Diaminobenzidine staining kit (Servicebio, Wuhan, China). .. The first step was same as IHC from antigen retrieval to secondary antibody incubation, and then repeated the step after washing the section with PBS (change the second primary antibody with S100A4 (Abcam) antibodies).

Incubation:

Article Title: Pg-Induced ATR Activation Promotes ESCC Progression via M2 TAM Polarization.
Article Snippet: Background: Emerging evidence suggests that oral pathogens may contribute to the development of systemic malignancies.. Porphyromonas gingivalis (Pg), a major periodontal pathogen, has been implicated in several cancers including esophageal squamous cell carcinoma (ESCC).. However, the molecular mechanisms underlying this association remain unclear.

Staining:

Article Title: MAO/SA composite coating on magnesium alloy: Corrosion resistance improvement and time-programmed Ca 2 + /Mg 2+ release for bone repair.
Article Snippet: Magnesium (Mg) alloys are attractive as orthopedic implants because of their favorable biofunctions, mechanical properties, and biodegradability.. However, their clinical applications are hindered by their rapid degradation rate in physiological environments, leading to inadequate functional and mechanical behavior of implants across different stages of bone repair.. Here, Mg alloys were firstly modified by micro-arc oxidation (MAO), and then sealed with Ca2+-crosslinked sodium alginate (SA) hydrogel coatings through different immersion cycles, constructing the MAO/SA composite coatings to further improve the corrosion resistance and simultaneously supply Ca2+ alongside Mg2+.

Article Title: Alginate-TEMPO conjugate hydrogel with synergistic antioxidant and angiogenic activities for accelerating wound healing.
Article Snippet: Effective management of cutaneous wounds remains a significant clinical challenge, often exacerbated by excessive reactive oxygen species (ROS) in the wound microenvironment.. This study developed a novel hydrogel for accelerated wound healing based on a covalent conjugate (Alg-T) of sodium alginate, a natural polysaccharide macromolecule, and a ROS scavenger 4-amino-TEMPO.. Two kinds of conjugates with grafting ratios of approximately 3% (Alg-T1) and 8% (Alg-T2) were successfully synthesized.

Recombinant:

Article Title: Robust transcriptomic hallmarks targeting intratumor heterogeneity in intrahepatic cholangiocarcinoma.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-CD66b Abcam Cat# ab197678; RRID: AB_3644234 Rabbit monoclonal anti-CD68 Cell Signaling Technology Cat# 76437; RRID: AB_2799882 Rabbit monoclonal anti-CD3 Abcam Cat# ab16669; RRID: AB_443425 Mouse monoclonal anti-CD20 Abcam Cat# ab9475; RRID: AB_307267 Rabbit anti-αSMA Servicebio Cat# GB111364; RRID: AB_2910228 Mouse anti-CK19 Servicebio Cat# GB12197; RRID: AB_2941910 Mouse monoclonal anti-GPRC5A Santa Cruz Biotechnology Cat# sc-390263 Rabbit anti-S100P Servicebio Cat# GB113949 Mouse monoclonal anti-CEACAM5 Bioass Cat# bsm-43141M Rabbit anti-MASPIN Servicebio Cat# GB111040 Rabbit polyclonal anti-PSCA Bioass Cat# bs-0669R; RRID: AB_10856299 Rabbit anti-MSLN Servicebio Cat# GB111016 Rabbit polyclonal anti-VTCN1 Abmart Cat# PK03217 Rabbit polyclonal anti-FXYD2 Abmart Cat# PH9090 Rabbit polyclonal anti-CHST9 Bioass Cat# bs-13940R Rabbit anti-ANXA9 Servicebio Cat# GB114601 Rabbit polyclonal anti-DCDC2 Bioass Cat# bs-11824R Rabbit polyclonal anti-ZBTB20 Abmart Cat# PHA9198 Goat polyclonal anti-CXCL5 R&D Systems Cat# AF254; RRID: AB_355281 Rabbit polyclonal anti-CXCL5 Abmart Cat# PH9257 Rabbit anti-GAPDH Servicebio Cat# GB11002; RRID: AB_2904017 Anti-mouse PD-1 Selleck Cat# A2122; RRID: AB_3644244 IgG2a isotype control Selleck Cat# A2123; RRID: AB_3644245 HRP conjugated Goat Anti-Mouse IgG Servicebio Cat# GB23301; RRID: AB_2904020 HRP conjugated Goat Anti-Rabbit IgG Servicebio Cat# GB23303; RRID: AB_2811189 HRP conjugated Rabbit Anti-Goat IgG Servicebio Cat# GB23304; RRID: AB_2938981 FVD APC/CY7 eBioscience Cat# 65-0865-14 CD45 AF700 BioLegend Cat# 109822; RRID: AB_493731 CD11b BV510 BioLegend Cat# 101263; RRID: AB_2629529 Ly6G FITC BioLegend Cat# 127605; RRID: AB_1236488 CD45 PE/Cy5 BioLegend Cat# 304009; RRID: AB_314397 CD66b APC BioLegend Cat# 305117; RRID: AB_2566606 Bacterial and virus strains PGMLV-CMV-Mouse_ Cxcl5-3×Flag-PGK-Puro Genomeditech, Shanghai N/A PGMLV- PGK-Puro Genomeditech, Shanghai N/A Biological samples Human iCCA tissue microarray 1 (used for immunohistochemistry) Zhongshan Hospital, Fudan University (Shanghai, China) Dong et al.8 Human iCCA tissue microarray 2 (used for immunohistochemistry) Zhongshan Hospital, Fudan University (Shanghai, China) Dong et al.8 2 healthy donors’ neutrophil Zhongshan Hospital, Fudan University (Shanghai, China) This paper (Continued on next page) e1 Cell Reports Medicine 7, 102708, April 21, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Recombinant human EGF Peprotech Cat# PHG0311 Insulin-Transferrin-SeleniumSodium Pyruvate Gibco Cat# 51300044 Ganetespib Selleck Cat# S1159 Alvespimycin Selleck Cat# S1142 TAE684 Selleck Cat# S1108 ABT-263 Selleck Cat# S1001 dBET6 Selleck Cat# S8762 EDTA buffer Servicebio Cat# G1203 Citrate buffer Servicebio Cat# G1202 Endogenous peroxidase blocking solution YIFAN Biological Cat# YFH4001 Goat serum BOSTER Cat# AR0009 Diaminobenzidine solution Servicebio Cat# G1212 Lysis buffer Biosharp Cat# BL504A Proteinase inhibitor cocktail Yeasen Cat# 20124ES Phosphorylated protease inhibitor cocktail Yeasen Cat# 20109ES Collagenase IV BioFroxx Cat# 2091GR001 Matrigel Corning Cat# 356231 Advanced DMEM/F12 Gibco Cat# 12634010 Penicillin-Streptomycin Gibco Cat# 15070063 Primocin InvivoGen Cat# ant-pm-2 Glutamax Gibco Cat# 35050061 HEPES Gibco Cat# 15630080 B-27 Supplement Gibco Cat# 17504044 N-2 Supplement Gibco Cat# 17502048 N-acetylcysteine Sigma Cat# A9165 R-spondin 1 Sino Biological Cat# 11083-HNAS Noggin Sino Biological Cat# 50688-M02H Nicotinamide Sigma Cat# N0636 Gastrin Sigma Cat# G9145 EGF Peprotech Cat# AF-100-15-500 FGF10 Peprotech Cat# 100-26-25 Forskolin MedChemExpress Cat# HY-15371 Y27632 Selleck Cat# S1049 A83-01 MedChemExpress Cat# HY-10432 TrypLE Express Gibco Cat# 12604021 Critical commercial assays Cell Counting Kit (CCK-8) Yeasen Cat# 40203ES76 Human Peripheral Blood Neutrophil Isolation Solution Kit Solarbio Cat# P9040 MolPure TRIeasy Plus Total RNA Kit Yeasen Cat# 19211ES Hifair III 1st Strand cDNA Synthesis SuperMix Yeasen Cat# 11141ES Hieff qPCR SYBR Green Master Mix Yeasen Cat# 11202ES All-in-One Tricine-PAGE Kit Epizyme Biomedical Technology Cat# PG310 (Continued on next page) Cell Reports Medicine 7, 102708, April 21, 2026 e2 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Omni-Easy Ready-to-use BCA Protein Assay Kit Epizyme Biomedical Technology Cat# ZJ102 Lipofectamine 3000 Invitrogen Cat# L3000001 RNAmini Kit Qiagen Cat# 74536 Deposited data RNA-seq data of the murine iCCA models This paper NGDC: PRJCA056718 Lin’s iCCA cohort Lin et al.1 NODE: OEP002560; https://www.biosino.org/ node/project/detail/OEP002560 Fu-iCCA cohort Dong et al.8 NODE: OEP001105; https://www.biosino.org/ node/project/detail/OEP001105 HRA004766 cohort Liu et al.33 GSA: HRA004766; https://ngdc.cncb.ac.cn/ search/specific?db=hra&q=HRA004766 OEP002768 cohort Deng et al.34 NODE: OEP002768; https://www.biosino.org/ node/project/detail/OEP002768 GSE89749 cohort Jusakul et al.35 GEO: GSE89749; https://www.ncbi.nlm.nih.gov/ geo/query/acc.cgi?acc=GSE89749 PRJCA007744 cohort Xue et al.21 GSA: PRJCA007744; https://ngdc.cncb.ac.cn/ bioproject/browse/PRJCA007744 HRA000863 cohort Song et al.20 GSA: HRA000863; https://ngdc.cncb.ac.cn/ gsa-human/browse/HRA000863 GSE151530 cohort Ma et al.22 GEO: GSE151530; https://www.ncbi.nlm.nih.gov/ geo/query/acc.cgi?acc=GSE151530 OEP003191 cohort Ji et al.42 NODE: OEP003191; https://www.biosino.org/node/ project/detail/OEP003191 GSE171443 cohort Martin-Serrano et al.14 GEO: GSE171443; https://www.ncbi.nlm.nih.gov/ geo/query/acc.cgi?acc=GSE171443 Experimental models: Cell lines HuCCT1 Dr. Daming Gao (Shanghai Institute of Biochemistry and Cell Biology) RRID: CVCL_0324 RBE Dr. Daming Gao (Shanghai Institute of Biochemistry and Cell Biology Cat# RCB1292; RRID: CVCL_4896 HCCC9810 Dr. Qiang Gao (Zhongshan Hospital) RRID: CVCL_6908 ICC2935 Dr. Qiang Gao (Zhongshan Hospital) Dong et al.8 ICC4715 Dr. Qiang Gao (Zhongshan Hospital) Dong et al.8 ICC39T Dr. Qiang Gao (Zhongshan Hospital) This paper KTP Dr. Qiang Gao (Zhongshan Hospital) This paper Experimental models: Organisms/strains Mouse: C57BL/6J mice GemPharmatech LLC.

Blocking Assay:

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Immunohistochemical staining:

Microscopy:

Article Title: Sarsasapogenin ameliorates Alzheimer's disease by dual inhibition of RIPK1-mediated necroptosis and pyroptosis.
Article Snippet: Sarsasapogenin (Sar), a natural bioactive steroidal saponin derived from Anemarrhena asphodeloides, has demonstrated significant neuroprotective effects in preclinical models of Alzheimer's disease (AD).. However, its specific mechanism of action, particularly in modulating receptor-interacting protein kinase 1 (RIPK1)-mediated necroptosis and pyroptosis, remains underexplored.. This study aimed to investigate Sar's therapeutic potential in AD by targeting RIPK1, a central regulator of programmed cell death.

Review