Review



bcip nbt colorimetric detection  (Santa Cruz Biotechnology)


Bioz Verified Symbol Santa Cruz Biotechnology is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Santa Cruz Biotechnology bcip nbt colorimetric detection
    Bcip Nbt Colorimetric Detection, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bcip/BCIP-NBT+Solution/pm29620139-113-5-8
    Average 93 stars, based on 41 article reviews
    bcip nbt colorimetric detection - by Bioz Stars, 2026-11
    93/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: COX2: A Prognostic Inflammogenic Marker Drives Cervical Carcinogenesis In Vivo Through NFκB/IAP/p53Axis
    Article Snippet: .. Blots were thereafter washed with TBST Buffer solution (TBS; tween 20) for 4 times and subsequently incubated with alkaline phosphatase conjugated secondary antibodies (GeneTex, 1:500dilutions in TBS) at 40C for 2h, followed by TBST washing (4times) and incubation with the chromogenic substrate 5-bromo, 4-chloro, 3- indoylphosphate/ Nitro-Blue tetrazolium (BCIP/NBT; SantaCruz Biotechnology, USA) for visualizing protein expressions in the form of bands. ..

    Article Title: Morin-VitaminE-β-CyclodextrinInclusionComplexLoadedChitosanNanoparticles (M-Vit.E-CD-CSNPs) Ameliorate Arsenic-Induced Hepatotoxicityina Murine Model
    Article Snippet: .. The membrane is blocked with 10% skimmed milk or 5% BSA and incubated overnight with primary antibody of different proteins and β-actin (1:2000 dilution; Santa Cruz, CA, USA), and next day after washing the membrane 3 times with wash buffer, was incubated with the alkaline phosphatase conjugated secondary antibody (1:5000 dilution) for 2 h. At last, protein expressions were detected using an NBT/BCIP solution. ..

    Article Title: Morin encapsulated chitosan nanoparticles (MCNPs) ameliorate arsenic induced liver damage through improvement of the antioxidant system and prevention of apoptosis and inflammation in mice
    Article Snippet: .. The membrane is blocked with 10% skimmed milk or 5% BSA and incubated overnight with primary antibodies of different proteins and β-actin (1 : 1000; Santa Cruz, CA, USA), and the next day, after washing the membrane 3 times with wash buffer, it was incubated with the alkaline phosphatase conjugated secondary antibody for 2 h. At last, protein expressions were detected using NBT/BCIP solution. ..

    Article Title: Melatonin targets ferroptosis through bimodal alteration of redox environment and cellular pathways in NAFLD model
    Article Snippet: .. After incubation with secondary antibodies for 1–2 h at ambient room temperature, then the detection was done using NBT/BCIP chromogenic substrate and the relative expression of the respective proteins were analysed using ImageJ software (U.S.A.). siRNA transfection NRF2 gene expression was knocked down by transfecting 1 × 106 HepG2 cells with siRNA-NRF2 (sc-37030, Santa Cruz, U.S.A.) or siRNA control. .. Lipofectamine 3000 (Invitrogen, U.S.A.) was used to perform transient siRNA transfection, according to the manual of the manufacturer.

    Membrane:

    Article Title: Morin-VitaminE-β-CyclodextrinInclusionComplexLoadedChitosanNanoparticles (M-Vit.E-CD-CSNPs) Ameliorate Arsenic-Induced Hepatotoxicityina Murine Model
    Article Snippet: .. The membrane is blocked with 10% skimmed milk or 5% BSA and incubated overnight with primary antibody of different proteins and β-actin (1:2000 dilution; Santa Cruz, CA, USA), and next day after washing the membrane 3 times with wash buffer, was incubated with the alkaline phosphatase conjugated secondary antibody (1:5000 dilution) for 2 h. At last, protein expressions were detected using an NBT/BCIP solution. ..

    Article Title: Morin encapsulated chitosan nanoparticles (MCNPs) ameliorate arsenic induced liver damage through improvement of the antioxidant system and prevention of apoptosis and inflammation in mice
    Article Snippet: .. The membrane is blocked with 10% skimmed milk or 5% BSA and incubated overnight with primary antibodies of different proteins and β-actin (1 : 1000; Santa Cruz, CA, USA), and the next day, after washing the membrane 3 times with wash buffer, it was incubated with the alkaline phosphatase conjugated secondary antibody for 2 h. At last, protein expressions were detected using NBT/BCIP solution. ..

    Activity Assay:

    Article Title: Modulation of angiogenic switch in reprogramming browning and lipid metabolism in white adipocytes.
    Article Snippet: Thermogenic activation via trans-and de novo browning of white adipocytes is a promising strategy to accelerate lipid metabolism for regulating obesity-related disorders.. In this study, we investigated the intricate interplay between angiogenic regulation and browning in white adipocytes using the bioactive compound, resveratrol (Rsv).. Rsv has previously been documented for its regulatory influence on the trans and de novo browning of white adipocytes.

    Expressing:

    Article Title: Melatonin targets ferroptosis through bimodal alteration of redox environment and cellular pathways in NAFLD model
    Article Snippet: .. After incubation with secondary antibodies for 1–2 h at ambient room temperature, then the detection was done using NBT/BCIP chromogenic substrate and the relative expression of the respective proteins were analysed using ImageJ software (U.S.A.). siRNA transfection NRF2 gene expression was knocked down by transfecting 1 × 106 HepG2 cells with siRNA-NRF2 (sc-37030, Santa Cruz, U.S.A.) or siRNA control. .. Lipofectamine 3000 (Invitrogen, U.S.A.) was used to perform transient siRNA transfection, according to the manual of the manufacturer.

    Transfection:

    Article Title: Melatonin targets ferroptosis through bimodal alteration of redox environment and cellular pathways in NAFLD model
    Article Snippet: .. After incubation with secondary antibodies for 1–2 h at ambient room temperature, then the detection was done using NBT/BCIP chromogenic substrate and the relative expression of the respective proteins were analysed using ImageJ software (U.S.A.). siRNA transfection NRF2 gene expression was knocked down by transfecting 1 × 106 HepG2 cells with siRNA-NRF2 (sc-37030, Santa Cruz, U.S.A.) or siRNA control. .. Lipofectamine 3000 (Invitrogen, U.S.A.) was used to perform transient siRNA transfection, according to the manual of the manufacturer.

    Gene Expression:

    Article Title: Melatonin targets ferroptosis through bimodal alteration of redox environment and cellular pathways in NAFLD model
    Article Snippet: .. After incubation with secondary antibodies for 1–2 h at ambient room temperature, then the detection was done using NBT/BCIP chromogenic substrate and the relative expression of the respective proteins were analysed using ImageJ software (U.S.A.). siRNA transfection NRF2 gene expression was knocked down by transfecting 1 × 106 HepG2 cells with siRNA-NRF2 (sc-37030, Santa Cruz, U.S.A.) or siRNA control. .. Lipofectamine 3000 (Invitrogen, U.S.A.) was used to perform transient siRNA transfection, according to the manual of the manufacturer.

    Control:

    Article Title: Melatonin targets ferroptosis through bimodal alteration of redox environment and cellular pathways in NAFLD model
    Article Snippet: .. After incubation with secondary antibodies for 1–2 h at ambient room temperature, then the detection was done using NBT/BCIP chromogenic substrate and the relative expression of the respective proteins were analysed using ImageJ software (U.S.A.). siRNA transfection NRF2 gene expression was knocked down by transfecting 1 × 106 HepG2 cells with siRNA-NRF2 (sc-37030, Santa Cruz, U.S.A.) or siRNA control. .. Lipofectamine 3000 (Invitrogen, U.S.A.) was used to perform transient siRNA transfection, according to the manual of the manufacturer.



    Similar Products

    94
    Vector Laboratories nbt bcip color reaction
    Nbt Bcip Color Reaction, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bcip/BCIP%2FNBT+Alkaline+Phosphatase+(AP)+Substrate+Kit%2C+5-bromo-4-chloro-3-indolyl+phosphate%2Fnitroblue+tetrazolium/pm42844293-45-2-5
    Average 94 stars, based on 1 article reviews
    nbt bcip color reaction - by Bioz Stars, 2026-11
    94/100 stars
      Buy from Supplier

    99
    Beyotime bcip nbt alkaline phosphatase staining kit
    Bcip Nbt Alkaline Phosphatase Staining Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bcip/BCIP-NBT+Alkaline+Phosphatase+Color+Development+Kit/pm42907415-89-28-33
    Average 99 stars, based on 1 article reviews
    bcip nbt alkaline phosphatase staining kit - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    99
    Beyotime bcip nbt alkaline phosphatase color development kit
    Bcip Nbt Alkaline Phosphatase Color Development Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bcip/BCIP-NBT+Alkaline+Phosphatase+Color+Development+Kit/pmc13657358-296-23-29
    Average 99 stars, based on 1 article reviews
    bcip nbt alkaline phosphatase color development kit - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    99
    Beyotime bcip nbt alp color development kit

    Bcip Nbt Alp Color Development Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bcip/BCIP-NBT+Alkaline+Phosphatase+Color+Development+Kit/pmc13681396-286-21-26
    Average 99 stars, based on 1 article reviews
    bcip nbt alp color development kit - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    99
    Beyotime bcip nbt staining kit

    Bcip Nbt Staining Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bcip/BCIP-NBT+Alkaline+Phosphatase+Color+Development+Kit/pm42893684-208-9-12
    Average 99 stars, based on 1 article reviews
    bcip nbt staining kit - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    99
    Beyotime nbt bcip chromogenic detection system

    Nbt Bcip Chromogenic Detection System, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bcip/BCIP-NBT+Alkaline+Phosphatase+Color+Development+Kit/pmc13686052-519-8-12
    Average 99 stars, based on 1 article reviews
    nbt bcip chromogenic detection system - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    99
    Beyotime 5 bromo 4 chloro 3 indolyl phosphate

    5 Bromo 4 Chloro 3 Indolyl Phosphate, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bcip/BCIP-NBT+Alkaline+Phosphatase+Color+Development+Kit/pmc13650031-92-8-17
    Average 99 stars, based on 1 article reviews
    5 bromo 4 chloro 3 indolyl phosphate - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    Image Search Results


    Journal: Stem Cell Reports

    Article Title: Diminished RELB impairs osteogenesis in mature periodontal ligament stem cells

    doi: 10.1016/j.stemcr.2026.103088

    Figure Lengend Snippet:

    Article Snippet: After 7 days of osteogenic induction, the cells were fixed in 4% paraformaldehyde for 30 min and then stained using a BCIP/NBT ALP color development kit (Beyotime, Shanghai, China, C3206) for 30 min at room temperature.

    Techniques: