bca protein quantitation assay (Keygen Biotech)

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Keygen Biotech bca protein quantitation assay
Bca Protein Quantitation Assay, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bca/assay+bca+kit+protein/pmc13254580-92-24-29
Average 86 stars, based on 1 article reviews

bca protein quantitation assay - by Bioz Stars, 2026-11

86/100 stars

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Bicinchoninic Acid Protein Assay:

Article Title: Lonicera japonica polysaccharide restores mucosal integrity in ulcerative colitis by increasing microbiota-derived spermidine.
Article Snippet: .. The BCA Protein Assay kit (Keygen Biotechnology, Nanjing, China) was used to determine protein content. .. Protein samples were separated by SDS-PAGE, transferred to a polyvinylidene fluoride (PVDF) membrane (Immobilon PVDF membrane, Merck Millipore, Burlington, MA, USA) and incubated with antibodies, including β-actin, p-65, p-p65, cleaved IL-1β, β-catenin, E-cadherin, ZO-1, occludin, HADHA, Hexokinase II, CK-18, IL-10, NLRP3, PPAR-γ, iNOS, cleaved caspase-1 (dilutions of 1:6000, 1:1000, 1:1000, 1:1000, 1:1000, 1:1000, 1:1000, 1:1000, 1:1000, 1:1000, 1:1500, 1:500, 1:1500, 1:1000, 1:1500, 1:1000).

Article Title: Transcription factor ID3 promotes fibroblast differentiation and proliferation in lung fibrosis through augmenting the TGF-β signaling pathway.
Article Snippet: Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive interstitial lung disease characterized by myofibroblast activation, accumulation, and excessive extracellular matrix deposition.. Although fibroblast activation and proliferation are central drivers of fibrogenesis, the molecular regulators coordinating these processes remain poorly defined.. This study investigated fibroblast lineage progression using single-cell RNA-sequencing data from fibroblast-enriched populations in bleomycin-induced mouse models and human IPF samples.

Article Title: Lonicera japonica polysaccharide restores mucosal integrity in ulcerative colitis by increasing microbiota-derived spermidine.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies p-p65 Affinity Biosciences Cat# AF2006; RRID: AB_2834435 p65 Santa Cruz Biotechnology Cat# sc-8008; RRID: AB_628017 HADHA Affinity Biosciences Cat# DF7288; RRID: AB_2839227 HADHB Wuhan Servicebio Technology Cat# GB114025; RRID: Not available Hexokinase II Affinity Biosciences Cat# DF6176; RRID: AB_2838143 PPAR-γ Affinity Biosciences Cat# AF6284; RRID: AB_2835135 NLRP3 Affinity Biosciences Cat# BF8029; RRID: AB_3083516 Cleaved caspase-1 Affinity Biosciences Cat# AF4022; RRID: AB_2845464 Cleaved IL-1β Affinity Biosciences Cat# AF4006; RRID: AB_2801567 IL-10 Affinity Biosciences Cat# DF6894; RRID: AB_2838853 iNOS Affinity Biosciences Cat# AF0199; RRID: AB_2833391 β-catenin Affinity Biosciences Cat# AF6266; RRID: AB_2835124 E-cadherin Affinity Biosciences Cat# AF0131; RRID: AB_2833315 ZO-1 Affinity Biosciences Cat# AF5145; RRID: AB_2837631 Occludin Affinity Biosciences Cat# DF7504; RRID: AB_2841004 Cytokeratin 18 Affinity Biosciences Cat# AF0191; RRID: AB_2833384 Ki67 Affinity Biosciences Cat# AF0198; RRID: AB_2834152 β-actin Affinity Biosciences Cat# T0022-HRP; RRID: AB_2844378 Goat anti-rabbit IgG (H + L), HRPconjugated Affinity Biosciences Cat# S0001; RRID: AB_2839429 Goat anti-mouse IgG (H + L), HRPconjugated Affinity Biosciences Cat# S0002; RRID: AB_2839430 Chemicals, peptides, and recombinant proteins WLJP-025p This paper; previously characterized https://doi.org/10.1016/j.carbpol.2022. .. 120053 Spermidine Shanghai Yuanye Bio-Technology Cat# B26020-100 mg Dextran sulfate sodium salt, DSS MP Biomedicals Cat# 160110 Lipopolysaccharide, LPS Sigma-Aldrich Cat# L2630 Thioglycollate medium Qingdao Hope Bio-Technology Cat# HBKD5190 NP-40 lysis buffer Beyotime Cat# P0013F Critical commercial assays Mouse IL-1β ELISA kit MultiSciences (Lianke) Biotech Cat# EK201B/3-24 Mouse IL-6 ELISA kit MultiSciences (Lianke) Biotech Cat# EK206/3-24 Mouse TNF-α ELISA kit MultiSciences (Lianke) Biotech Cat# EK282/4-24 Mouse IFN-γ ELISA kit MultiSciences (Lianke) Biotech Cat# EK280/3-24 BCA Protein Assay Kit Keygen Biotechnology Cat# KGP903 L-lactic acid assay kit Nanjing Jiancheng Bioengineering Institute Cat# A019-2-2 FAO Colorimetric Assay Kit Elabscience Cat# E-BC-K784-M Seahorse XF long-chain fatty acid oxidation substrate oxidation stress test kit Agilent Technologies Cat# 103672-100 Organoid culture kit/medium Guangzhou Kinlogix Biotechnology OG03-MN; OG-M2 Deposited data 2bRAD-M sequencing data from mouse feces This paper NCBI SRA/BioProject: PRJNA1252706 (Continued on next page) Cell Reports 45, 117554, June 23, 2026 17 ..

other:

Article Title: A cell-free lipid sterosomal therapeutic enables bone regeneration via USP18-mediated noncanonical S1P signaling
Article Snippet: Hematoxylin-eosin (H&E) staining kit, Masson's trichrome staining kit, calcein-AM/PI double staining kit, and CCK-8 kit were purchased from Solarbio (Beijing, China).

Article Title: HFPO-TA inhibits testosterone synthesis by triggering FTO-mediated m 6 A modification to drive NCOA4-associated ferroptosis.
Article Snippet: Total proteins from testicular tissues and TM3 cells were extracted using a commercial kit (KeyGEN, KGB5303) and quantified using the BCA assay (KeyGEN, KGB2101).

Protein Concentration:

Article Title: Targeted activation of PPARG or AKT1 alleviates liver injury in mice with type 2 diabetes and sepsis by modulating inflammatory and metabolic pathways.
Article Snippet: .. The BCA Quantification Kit (KGB2101-50, KeyGen Biotech, Jiangsu, China) was adopted for measuring the protein concentration. ..

Article Title: Daphnetin alleviates neuropathic pain in rats by modulating spinal microglial polarization via the Nrf2/NLRP3 inflammasome pathway.
Article Snippet: Background: Neuropathic pain (NP) is a frequent sequel to peripheral nerve injury and maladaptive nervous system function.. Daphnetin (DAP), a bioactive 7,8-dihydroxycoumarin derived from the traditional Chinese medical plant Daphne giraldii Nitsche, has potential anti-nociceptive effects and alleviated NP following intrathecal injection.. However, the molecular target of DAP for this action remains unknown.

BIA-KA:

Article Title: Activation of the Jak2/Stat3 pathway by ROS-dependent signaling cascades initiates hepatitis B virus-induced hepatic inflammatory responses
Article Snippet: .. The total protein of cells or liver tissues was extracted using the Whole Cell Lysis Assay (KGP250, KeyGen Biotech, China) and quantified by the BCA Protein Quantitation Assay (KGPBCA, KeyGen Biotech, China). .. The sample proteins were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore Corporation, USA).

Lysis:

Article Title: Lonicera japonica polysaccharide restores mucosal integrity in ulcerative colitis by increasing microbiota-derived spermidine.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies p-p65 Affinity Biosciences Cat# AF2006; RRID: AB_2834435 p65 Santa Cruz Biotechnology Cat# sc-8008; RRID: AB_628017 HADHA Affinity Biosciences Cat# DF7288; RRID: AB_2839227 HADHB Wuhan Servicebio Technology Cat# GB114025; RRID: Not available Hexokinase II Affinity Biosciences Cat# DF6176; RRID: AB_2838143 PPAR-γ Affinity Biosciences Cat# AF6284; RRID: AB_2835135 NLRP3 Affinity Biosciences Cat# BF8029; RRID: AB_3083516 Cleaved caspase-1 Affinity Biosciences Cat# AF4022; RRID: AB_2845464 Cleaved IL-1β Affinity Biosciences Cat# AF4006; RRID: AB_2801567 IL-10 Affinity Biosciences Cat# DF6894; RRID: AB_2838853 iNOS Affinity Biosciences Cat# AF0199; RRID: AB_2833391 β-catenin Affinity Biosciences Cat# AF6266; RRID: AB_2835124 E-cadherin Affinity Biosciences Cat# AF0131; RRID: AB_2833315 ZO-1 Affinity Biosciences Cat# AF5145; RRID: AB_2837631 Occludin Affinity Biosciences Cat# DF7504; RRID: AB_2841004 Cytokeratin 18 Affinity Biosciences Cat# AF0191; RRID: AB_2833384 Ki67 Affinity Biosciences Cat# AF0198; RRID: AB_2834152 β-actin Affinity Biosciences Cat# T0022-HRP; RRID: AB_2844378 Goat anti-rabbit IgG (H + L), HRPconjugated Affinity Biosciences Cat# S0001; RRID: AB_2839429 Goat anti-mouse IgG (H + L), HRPconjugated Affinity Biosciences Cat# S0002; RRID: AB_2839430 Chemicals, peptides, and recombinant proteins WLJP-025p This paper; previously characterized https://doi.org/10.1016/j.carbpol.2022. .. 120053 Spermidine Shanghai Yuanye Bio-Technology Cat# B26020-100 mg Dextran sulfate sodium salt, DSS MP Biomedicals Cat# 160110 Lipopolysaccharide, LPS Sigma-Aldrich Cat# L2630 Thioglycollate medium Qingdao Hope Bio-Technology Cat# HBKD5190 NP-40 lysis buffer Beyotime Cat# P0013F Critical commercial assays Mouse IL-1β ELISA kit MultiSciences (Lianke) Biotech Cat# EK201B/3-24 Mouse IL-6 ELISA kit MultiSciences (Lianke) Biotech Cat# EK206/3-24 Mouse TNF-α ELISA kit MultiSciences (Lianke) Biotech Cat# EK282/4-24 Mouse IFN-γ ELISA kit MultiSciences (Lianke) Biotech Cat# EK280/3-24 BCA Protein Assay Kit Keygen Biotechnology Cat# KGP903 L-lactic acid assay kit Nanjing Jiancheng Bioengineering Institute Cat# A019-2-2 FAO Colorimetric Assay Kit Elabscience Cat# E-BC-K784-M Seahorse XF long-chain fatty acid oxidation substrate oxidation stress test kit Agilent Technologies Cat# 103672-100 Organoid culture kit/medium Guangzhou Kinlogix Biotechnology OG03-MN; OG-M2 Deposited data 2bRAD-M sequencing data from mouse feces This paper NCBI SRA/BioProject: PRJNA1252706 (Continued on next page) Cell Reports 45, 117554, June 23, 2026 17 ..

Enzyme-linked Immunosorbent Assay:

Article Title: Lonicera japonica polysaccharide restores mucosal integrity in ulcerative colitis by increasing microbiota-derived spermidine.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies p-p65 Affinity Biosciences Cat# AF2006; RRID: AB_2834435 p65 Santa Cruz Biotechnology Cat# sc-8008; RRID: AB_628017 HADHA Affinity Biosciences Cat# DF7288; RRID: AB_2839227 HADHB Wuhan Servicebio Technology Cat# GB114025; RRID: Not available Hexokinase II Affinity Biosciences Cat# DF6176; RRID: AB_2838143 PPAR-γ Affinity Biosciences Cat# AF6284; RRID: AB_2835135 NLRP3 Affinity Biosciences Cat# BF8029; RRID: AB_3083516 Cleaved caspase-1 Affinity Biosciences Cat# AF4022; RRID: AB_2845464 Cleaved IL-1β Affinity Biosciences Cat# AF4006; RRID: AB_2801567 IL-10 Affinity Biosciences Cat# DF6894; RRID: AB_2838853 iNOS Affinity Biosciences Cat# AF0199; RRID: AB_2833391 β-catenin Affinity Biosciences Cat# AF6266; RRID: AB_2835124 E-cadherin Affinity Biosciences Cat# AF0131; RRID: AB_2833315 ZO-1 Affinity Biosciences Cat# AF5145; RRID: AB_2837631 Occludin Affinity Biosciences Cat# DF7504; RRID: AB_2841004 Cytokeratin 18 Affinity Biosciences Cat# AF0191; RRID: AB_2833384 Ki67 Affinity Biosciences Cat# AF0198; RRID: AB_2834152 β-actin Affinity Biosciences Cat# T0022-HRP; RRID: AB_2844378 Goat anti-rabbit IgG (H + L), HRPconjugated Affinity Biosciences Cat# S0001; RRID: AB_2839429 Goat anti-mouse IgG (H + L), HRPconjugated Affinity Biosciences Cat# S0002; RRID: AB_2839430 Chemicals, peptides, and recombinant proteins WLJP-025p This paper; previously characterized https://doi.org/10.1016/j.carbpol.2022. .. 120053 Spermidine Shanghai Yuanye Bio-Technology Cat# B26020-100 mg Dextran sulfate sodium salt, DSS MP Biomedicals Cat# 160110 Lipopolysaccharide, LPS Sigma-Aldrich Cat# L2630 Thioglycollate medium Qingdao Hope Bio-Technology Cat# HBKD5190 NP-40 lysis buffer Beyotime Cat# P0013F Critical commercial assays Mouse IL-1β ELISA kit MultiSciences (Lianke) Biotech Cat# EK201B/3-24 Mouse IL-6 ELISA kit MultiSciences (Lianke) Biotech Cat# EK206/3-24 Mouse TNF-α ELISA kit MultiSciences (Lianke) Biotech Cat# EK282/4-24 Mouse IFN-γ ELISA kit MultiSciences (Lianke) Biotech Cat# EK280/3-24 BCA Protein Assay Kit Keygen Biotechnology Cat# KGP903 L-lactic acid assay kit Nanjing Jiancheng Bioengineering Institute Cat# A019-2-2 FAO Colorimetric Assay Kit Elabscience Cat# E-BC-K784-M Seahorse XF long-chain fatty acid oxidation substrate oxidation stress test kit Agilent Technologies Cat# 103672-100 Organoid culture kit/medium Guangzhou Kinlogix Biotechnology OG03-MN; OG-M2 Deposited data 2bRAD-M sequencing data from mouse feces This paper NCBI SRA/BioProject: PRJNA1252706 (Continued on next page) Cell Reports 45, 117554, June 23, 2026 17 ..

Acid Assay:

Article Title: Lonicera japonica polysaccharide restores mucosal integrity in ulcerative colitis by increasing microbiota-derived spermidine.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies p-p65 Affinity Biosciences Cat# AF2006; RRID: AB_2834435 p65 Santa Cruz Biotechnology Cat# sc-8008; RRID: AB_628017 HADHA Affinity Biosciences Cat# DF7288; RRID: AB_2839227 HADHB Wuhan Servicebio Technology Cat# GB114025; RRID: Not available Hexokinase II Affinity Biosciences Cat# DF6176; RRID: AB_2838143 PPAR-γ Affinity Biosciences Cat# AF6284; RRID: AB_2835135 NLRP3 Affinity Biosciences Cat# BF8029; RRID: AB_3083516 Cleaved caspase-1 Affinity Biosciences Cat# AF4022; RRID: AB_2845464 Cleaved IL-1β Affinity Biosciences Cat# AF4006; RRID: AB_2801567 IL-10 Affinity Biosciences Cat# DF6894; RRID: AB_2838853 iNOS Affinity Biosciences Cat# AF0199; RRID: AB_2833391 β-catenin Affinity Biosciences Cat# AF6266; RRID: AB_2835124 E-cadherin Affinity Biosciences Cat# AF0131; RRID: AB_2833315 ZO-1 Affinity Biosciences Cat# AF5145; RRID: AB_2837631 Occludin Affinity Biosciences Cat# DF7504; RRID: AB_2841004 Cytokeratin 18 Affinity Biosciences Cat# AF0191; RRID: AB_2833384 Ki67 Affinity Biosciences Cat# AF0198; RRID: AB_2834152 β-actin Affinity Biosciences Cat# T0022-HRP; RRID: AB_2844378 Goat anti-rabbit IgG (H + L), HRPconjugated Affinity Biosciences Cat# S0001; RRID: AB_2839429 Goat anti-mouse IgG (H + L), HRPconjugated Affinity Biosciences Cat# S0002; RRID: AB_2839430 Chemicals, peptides, and recombinant proteins WLJP-025p This paper; previously characterized https://doi.org/10.1016/j.carbpol.2022. .. 120053 Spermidine Shanghai Yuanye Bio-Technology Cat# B26020-100 mg Dextran sulfate sodium salt, DSS MP Biomedicals Cat# 160110 Lipopolysaccharide, LPS Sigma-Aldrich Cat# L2630 Thioglycollate medium Qingdao Hope Bio-Technology Cat# HBKD5190 NP-40 lysis buffer Beyotime Cat# P0013F Critical commercial assays Mouse IL-1β ELISA kit MultiSciences (Lianke) Biotech Cat# EK201B/3-24 Mouse IL-6 ELISA kit MultiSciences (Lianke) Biotech Cat# EK206/3-24 Mouse TNF-α ELISA kit MultiSciences (Lianke) Biotech Cat# EK282/4-24 Mouse IFN-γ ELISA kit MultiSciences (Lianke) Biotech Cat# EK280/3-24 BCA Protein Assay Kit Keygen Biotechnology Cat# KGP903 L-lactic acid assay kit Nanjing Jiancheng Bioengineering Institute Cat# A019-2-2 FAO Colorimetric Assay Kit Elabscience Cat# E-BC-K784-M Seahorse XF long-chain fatty acid oxidation substrate oxidation stress test kit Agilent Technologies Cat# 103672-100 Organoid culture kit/medium Guangzhou Kinlogix Biotechnology OG03-MN; OG-M2 Deposited data 2bRAD-M sequencing data from mouse feces This paper NCBI SRA/BioProject: PRJNA1252706 (Continued on next page) Cell Reports 45, 117554, June 23, 2026 17 ..

Colorimetric Assay:

Article Title: Lonicera japonica polysaccharide restores mucosal integrity in ulcerative colitis by increasing microbiota-derived spermidine.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies p-p65 Affinity Biosciences Cat# AF2006; RRID: AB_2834435 p65 Santa Cruz Biotechnology Cat# sc-8008; RRID: AB_628017 HADHA Affinity Biosciences Cat# DF7288; RRID: AB_2839227 HADHB Wuhan Servicebio Technology Cat# GB114025; RRID: Not available Hexokinase II Affinity Biosciences Cat# DF6176; RRID: AB_2838143 PPAR-γ Affinity Biosciences Cat# AF6284; RRID: AB_2835135 NLRP3 Affinity Biosciences Cat# BF8029; RRID: AB_3083516 Cleaved caspase-1 Affinity Biosciences Cat# AF4022; RRID: AB_2845464 Cleaved IL-1β Affinity Biosciences Cat# AF4006; RRID: AB_2801567 IL-10 Affinity Biosciences Cat# DF6894; RRID: AB_2838853 iNOS Affinity Biosciences Cat# AF0199; RRID: AB_2833391 β-catenin Affinity Biosciences Cat# AF6266; RRID: AB_2835124 E-cadherin Affinity Biosciences Cat# AF0131; RRID: AB_2833315 ZO-1 Affinity Biosciences Cat# AF5145; RRID: AB_2837631 Occludin Affinity Biosciences Cat# DF7504; RRID: AB_2841004 Cytokeratin 18 Affinity Biosciences Cat# AF0191; RRID: AB_2833384 Ki67 Affinity Biosciences Cat# AF0198; RRID: AB_2834152 β-actin Affinity Biosciences Cat# T0022-HRP; RRID: AB_2844378 Goat anti-rabbit IgG (H + L), HRPconjugated Affinity Biosciences Cat# S0001; RRID: AB_2839429 Goat anti-mouse IgG (H + L), HRPconjugated Affinity Biosciences Cat# S0002; RRID: AB_2839430 Chemicals, peptides, and recombinant proteins WLJP-025p This paper; previously characterized https://doi.org/10.1016/j.carbpol.2022. .. 120053 Spermidine Shanghai Yuanye Bio-Technology Cat# B26020-100 mg Dextran sulfate sodium salt, DSS MP Biomedicals Cat# 160110 Lipopolysaccharide, LPS Sigma-Aldrich Cat# L2630 Thioglycollate medium Qingdao Hope Bio-Technology Cat# HBKD5190 NP-40 lysis buffer Beyotime Cat# P0013F Critical commercial assays Mouse IL-1β ELISA kit MultiSciences (Lianke) Biotech Cat# EK201B/3-24 Mouse IL-6 ELISA kit MultiSciences (Lianke) Biotech Cat# EK206/3-24 Mouse TNF-α ELISA kit MultiSciences (Lianke) Biotech Cat# EK282/4-24 Mouse IFN-γ ELISA kit MultiSciences (Lianke) Biotech Cat# EK280/3-24 BCA Protein Assay Kit Keygen Biotechnology Cat# KGP903 L-lactic acid assay kit Nanjing Jiancheng Bioengineering Institute Cat# A019-2-2 FAO Colorimetric Assay Kit Elabscience Cat# E-BC-K784-M Seahorse XF long-chain fatty acid oxidation substrate oxidation stress test kit Agilent Technologies Cat# 103672-100 Organoid culture kit/medium Guangzhou Kinlogix Biotechnology OG03-MN; OG-M2 Deposited data 2bRAD-M sequencing data from mouse feces This paper NCBI SRA/BioProject: PRJNA1252706 (Continued on next page) Cell Reports 45, 117554, June 23, 2026 17 ..

Sequencing:

Article Title: Lonicera japonica polysaccharide restores mucosal integrity in ulcerative colitis by increasing microbiota-derived spermidine.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies p-p65 Affinity Biosciences Cat# AF2006; RRID: AB_2834435 p65 Santa Cruz Biotechnology Cat# sc-8008; RRID: AB_628017 HADHA Affinity Biosciences Cat# DF7288; RRID: AB_2839227 HADHB Wuhan Servicebio Technology Cat# GB114025; RRID: Not available Hexokinase II Affinity Biosciences Cat# DF6176; RRID: AB_2838143 PPAR-γ Affinity Biosciences Cat# AF6284; RRID: AB_2835135 NLRP3 Affinity Biosciences Cat# BF8029; RRID: AB_3083516 Cleaved caspase-1 Affinity Biosciences Cat# AF4022; RRID: AB_2845464 Cleaved IL-1β Affinity Biosciences Cat# AF4006; RRID: AB_2801567 IL-10 Affinity Biosciences Cat# DF6894; RRID: AB_2838853 iNOS Affinity Biosciences Cat# AF0199; RRID: AB_2833391 β-catenin Affinity Biosciences Cat# AF6266; RRID: AB_2835124 E-cadherin Affinity Biosciences Cat# AF0131; RRID: AB_2833315 ZO-1 Affinity Biosciences Cat# AF5145; RRID: AB_2837631 Occludin Affinity Biosciences Cat# DF7504; RRID: AB_2841004 Cytokeratin 18 Affinity Biosciences Cat# AF0191; RRID: AB_2833384 Ki67 Affinity Biosciences Cat# AF0198; RRID: AB_2834152 β-actin Affinity Biosciences Cat# T0022-HRP; RRID: AB_2844378 Goat anti-rabbit IgG (H + L), HRPconjugated Affinity Biosciences Cat# S0001; RRID: AB_2839429 Goat anti-mouse IgG (H + L), HRPconjugated Affinity Biosciences Cat# S0002; RRID: AB_2839430 Chemicals, peptides, and recombinant proteins WLJP-025p This paper; previously characterized https://doi.org/10.1016/j.carbpol.2022. .. 120053 Spermidine Shanghai Yuanye Bio-Technology Cat# B26020-100 mg Dextran sulfate sodium salt, DSS MP Biomedicals Cat# 160110 Lipopolysaccharide, LPS Sigma-Aldrich Cat# L2630 Thioglycollate medium Qingdao Hope Bio-Technology Cat# HBKD5190 NP-40 lysis buffer Beyotime Cat# P0013F Critical commercial assays Mouse IL-1β ELISA kit MultiSciences (Lianke) Biotech Cat# EK201B/3-24 Mouse IL-6 ELISA kit MultiSciences (Lianke) Biotech Cat# EK206/3-24 Mouse TNF-α ELISA kit MultiSciences (Lianke) Biotech Cat# EK282/4-24 Mouse IFN-γ ELISA kit MultiSciences (Lianke) Biotech Cat# EK280/3-24 BCA Protein Assay Kit Keygen Biotechnology Cat# KGP903 L-lactic acid assay kit Nanjing Jiancheng Bioengineering Institute Cat# A019-2-2 FAO Colorimetric Assay Kit Elabscience Cat# E-BC-K784-M Seahorse XF long-chain fatty acid oxidation substrate oxidation stress test kit Agilent Technologies Cat# 103672-100 Organoid culture kit/medium Guangzhou Kinlogix Biotechnology OG03-MN; OG-M2 Deposited data 2bRAD-M sequencing data from mouse feces This paper NCBI SRA/BioProject: PRJNA1252706 (Continued on next page) Cell Reports 45, 117554, June 23, 2026 17 ..

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