bca protein quantitative kit (DBI Bioscience)

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DBI Bioscience bca protein quantitative kit
Bca Protein Quantitative Kit, supplied by DBI Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bca+quantitation+kit/bca+protein+quantitative+kit/pmc05755160-85-5-9
Average 90 stars, based on 1 article reviews

bca protein quantitative kit - by Bioz Stars, 2026-09

90/100 stars

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Protein Quantitation:

Article Title: Overexpression of NEDD9 in renal cell carcinoma is associated with tumor migration and invasion
Article Snippet: Total proteins extracted from frozen tissues and cells lines transfected for 72 h and then incubated with RIPA lysis buffer (DBI Bioscience, Shanghai, China), following the manufacturer's protocol. .. After protein quantitation using a BCA Protein Quantitative kit (DBI Bioscience), 100 μg protein per lane were separated by SDS-PAGE (10% gel) and blotted onto polyvinylidene difluoride membranes. .. The membranes were blocked with 5% skim milk and then incubated overnight at 4°C with anti-NEDD9 (1:1,000; cat. no. ab37161; Abcam) or anti-tubulin (cat. no. sc-8035; 1:1,000; Santa Cruz Biotechnologies, Inc., Dallas, TX, USA), followed by incubation with horseradish peroxidase-conjugated IgG goat anti-rabbit H&L; (cat. no. ab97051; 1:2,000; Abcam) and goat anti-mouse IgG H&L (cat. no. ab6708; 1:2,000; Abcam) at room temperature for 1 h. An enhanced chemiluminescence kit (Merck KGaA) was used for detection using FluorChemTM Q software (version 3.4.0.0; Protein Simple, San Jose, CA, USA).

Article Title: siRNA Suppression of NEDD9 Inhibits Proliferation and Enhances Apoptosis in Renal Cell Carcinoma
Article Snippet: Total cellular protein was extracted from cell lines transfected for 72 h using RIPA lysis buffer (DBI Bioscience, Shanghai, China) following the manufacturer’s instructions. .. After protein quantitation using BCA Protein Quantitative Kit (DBI Bioscience), equal amounts of proteins were separated by SDS-PAGE and blotted onto to PVDF membranes (0.45 μm; Millipore, Billerica, MA, USA). .. The membranes were blocked in 5% skim milk and then incubated overnight at 4°C with anti-NEDD9 (1:1,000; Abcam) or anti-tubulin (Santa Cruz Biotechnologies, Santa Cruz, CA, USA), followed by incubation with horseradish peroxidase-conjugated IgG.

SDS Page:

Protein Concentration:

Article Title: Effect of Liraglutide on endoplasmic reticulum stress in diabetes.
Article Snippet: Accepted Manuscript Effect of Liraglutide on endoplasmic reticulum stress in diabetes Linlin Zhao, Hao Guo, Hong Chen, Robert B. Petersen, Ling Zheng, Anlin Peng, Kun Huang PII: S0006-291X(13)01686-0 DOI: http://dx.doi.org/10.1016/j.bbrc.2013.10.026 Reference: YBBRC 31002 To appear in: Biochemical and Biophysical Research Communications Received Date: 4 October 2013 Please cite this article as: L. Zhao, H. Guo, H. Chen, R.B.. Petersen, L. Zheng, A. Peng, K. Huang, Effect of Liraglutide on endoplasmic reticulum stress in diabetes, Biochemical and Biophysical Research Communications (2013), doi: http://dx.doi.org/10.1016/j.bbrc.2013.10.026 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

Article Title: Overexpression of reptin in renal cell carcinoma contributes to tumor malignancies and its inhibition triggers senescence of cancer cells.
Article Snippet: Objectives: Reptin is an AAA ATPase associated with several complexes involved in chromatin remodeling, transcriptional regulation, DNA damage repair, and telomerase activity.. Functional studies have implicated reptin in many cellular processes highly relevant to cancer.. In this study, we investigated reptin expression in renal cell carcinoma (RCC) and its biologic functions in RCC cells.

Bicinchoninic Acid Protein Assay:

other:

Article Title: siRNA Suppression of NEDD9 Inhibits Proliferation and Enhances Apoptosis in Renal Cell Carcinoma
Article Snippet: After protein quantitation using BCA Protein Quan titative Kit (DBI Bioscience), equal amounts of proteins were separated by SDS-PAGE and blotted onto to PVDF membranes (0.45 μm; Millipore, Billerica, MA, USA).

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