bca protein array kit (Thermo Fisher)
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Bca Protein Array Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bca+protein+array+kit/BCA+Protein+Assay+Kit/pm41390720-163-24-28
Average 99 stars, based on 1 article reviews
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Bicinchoninic Acid Protein Assay:Article Title: Countering postoperative immune suppression with a self-assembling dendritic cell nanovaccine Article Snippet: .. Materials : Pierce BIA-KA:Article Title: Kinase-independent signaling by PIM1 promotes drug resistance by increasing mitophagy and reducing oxidative stress. Article Snippet: .. Protein quantification was determined with Pierce Article Title: Polymeric alpha-1 antitrypsin perturbs Paneth cell proteostasis and exacerbates intestinal inflammation. Article Snippet: .. Coimmunoprecipitation assay Intestinal crypt lysates were prepared in cold RIPA lysis buffer (50 mM Tris-HCl, 150mM NaCl, 1% Triton X-100, 1 mM EDTA, 0.1% SDS, pH 7.4) in the presence of protease and phosphatase inhibitors, incubated for 30 min on ice, and centrifuged at 16,000 rcf for 15 min. Supernatants were collected, and the protein concentration was determined with a Pierce Article Title: RhoA through its various effectors mediate mitochondrial fragmentation in polycystic kidney disease. Article Snippet: .. Cell lysis buffer contained 30 mM HEPES, pH 7.4, 100 mM NaCl, 1% Triton X-100, 20 mM NaF, 1 mM EGTA, 1 mM Na3V_O4 (Cell Signaling), 1 mM PMSF (Bioshop), protease inhibitors (Roche), phosphatase inhibitors (PhosSTOP, Roche); and protein content was measured by Article Title: An epigenetic program dictates MYC amplification dynamics that promote transient, extrachromosomal, and inherited genomic events. Article Snippet: .. The resulting pellet was lysed in IPH buffer (50 mM Tris-HCl, pH 8.0; 150 mM NaCl; 5 mM EDTA; 0.5% NP-40; 10% glycerol; with protease and phosphatase inhibitors) and sonicated with the Qsonica Q800R2 system (Qsonica) at 70% amplitude 30sec on 30sec off setting for 20 min. Lysates were clarified by centrifugation at maximum speed for 10 min at 4 ◦C and protein concentrations determined using the Pierce Article Title: Opisthorchis viverrini infection is associated with kidney injury involving excretory–secretory product-mediated inflammation, oxidative stress, and hypoxia Article Snippet: .. Article in Press Scientific Reports https://doi.org/10.1038/s41598-026-71415-3 Total protein concentration was determined using the bicinchoninic acid assay with the Pierce Co-Immunoprecipitation Assay:Article Title: Polymeric alpha-1 antitrypsin perturbs Paneth cell proteostasis and exacerbates intestinal inflammation. Article Snippet: .. Coimmunoprecipitation assay Intestinal crypt lysates were prepared in cold RIPA lysis buffer (50 mM Tris-HCl, 150mM NaCl, 1% Triton X-100, 1 mM EDTA, 0.1% SDS, pH 7.4) in the presence of protease and phosphatase inhibitors, incubated for 30 min on ice, and centrifuged at 16,000 rcf for 15 min. Supernatants were collected, and the protein concentration was determined with a Pierce Lysis:Article Title: Polymeric alpha-1 antitrypsin perturbs Paneth cell proteostasis and exacerbates intestinal inflammation. Article Snippet: .. Coimmunoprecipitation assay Intestinal crypt lysates were prepared in cold RIPA lysis buffer (50 mM Tris-HCl, 150mM NaCl, 1% Triton X-100, 1 mM EDTA, 0.1% SDS, pH 7.4) in the presence of protease and phosphatase inhibitors, incubated for 30 min on ice, and centrifuged at 16,000 rcf for 15 min. Supernatants were collected, and the protein concentration was determined with a Pierce Article Title: RhoA through its various effectors mediate mitochondrial fragmentation in polycystic kidney disease. Article Snippet: .. Cell lysis buffer contained 30 mM HEPES, pH 7.4, 100 mM NaCl, 1% Triton X-100, 20 mM NaF, 1 mM EGTA, 1 mM Na3V_O4 (Cell Signaling), 1 mM PMSF (Bioshop), protease inhibitors (Roche), phosphatase inhibitors (PhosSTOP, Roche); and protein content was measured by Incubation:Article Title: Polymeric alpha-1 antitrypsin perturbs Paneth cell proteostasis and exacerbates intestinal inflammation. Article Snippet: .. Coimmunoprecipitation assay Intestinal crypt lysates were prepared in cold RIPA lysis buffer (50 mM Tris-HCl, 150mM NaCl, 1% Triton X-100, 1 mM EDTA, 0.1% SDS, pH 7.4) in the presence of protease and phosphatase inhibitors, incubated for 30 min on ice, and centrifuged at 16,000 rcf for 15 min. Supernatants were collected, and the protein concentration was determined with a Pierce Protein Concentration:Article Title: Polymeric alpha-1 antitrypsin perturbs Paneth cell proteostasis and exacerbates intestinal inflammation. Article Snippet: .. Coimmunoprecipitation assay Intestinal crypt lysates were prepared in cold RIPA lysis buffer (50 mM Tris-HCl, 150mM NaCl, 1% Triton X-100, 1 mM EDTA, 0.1% SDS, pH 7.4) in the presence of protease and phosphatase inhibitors, incubated for 30 min on ice, and centrifuged at 16,000 rcf for 15 min. Supernatants were collected, and the protein concentration was determined with a Pierce Article Title: Opisthorchis viverrini infection is associated with kidney injury involving excretory–secretory product-mediated inflammation, oxidative stress, and hypoxia Article Snippet: .. Article in Press Scientific Reports https://doi.org/10.1038/s41598-026-71415-3 Total protein concentration was determined using the bicinchoninic acid assay with the Pierce other:Article Title: Vitamin D alleviates intracerebral hemorrhage symptoms by modulating pro-inflammatory/anti-inflammatory phenotypic transformation of microglia through P2X7R activation of NLRP3 Article Snippet: The protein concentration was determined using a Article Title: Transmembrane PhoxID: photoproximity labelling across the plasma membrane in vivo Article Snippet: Following Cell-Signaling:Article Title: RhoA through its various effectors mediate mitochondrial fragmentation in polycystic kidney disease. Article Snippet: .. Cell lysis buffer contained 30 mM HEPES, pH 7.4, 100 mM NaCl, 1% Triton X-100, 20 mM NaF, 1 mM EGTA, 1 mM Na3V_O4 (Cell Signaling), 1 mM PMSF (Bioshop), protease inhibitors (Roche), phosphatase inhibitors (PhosSTOP, Roche); and protein content was measured by Sonication:Article Title: An epigenetic program dictates MYC amplification dynamics that promote transient, extrachromosomal, and inherited genomic events. Article Snippet: .. The resulting pellet was lysed in IPH buffer (50 mM Tris-HCl, pH 8.0; 150 mM NaCl; 5 mM EDTA; 0.5% NP-40; 10% glycerol; with protease and phosphatase inhibitors) and sonicated with the Qsonica Q800R2 system (Qsonica) at 70% amplitude 30sec on 30sec off setting for 20 min. Lysates were clarified by centrifugation at maximum speed for 10 min at 4 ◦C and protein concentrations determined using the Pierce Centrifugation:Article Title: An epigenetic program dictates MYC amplification dynamics that promote transient, extrachromosomal, and inherited genomic events. Article Snippet: .. The resulting pellet was lysed in IPH buffer (50 mM Tris-HCl, pH 8.0; 150 mM NaCl; 5 mM EDTA; 0.5% NP-40; 10% glycerol; with protease and phosphatase inhibitors) and sonicated with the Qsonica Q800R2 system (Qsonica) at 70% amplitude 30sec on 30sec off setting for 20 min. Lysates were clarified by centrifugation at maximum speed for 10 min at 4 ◦C and protein concentrations determined using the Pierce Acid Assay:Article Title: Opisthorchis viverrini infection is associated with kidney injury involving excretory–secretory product-mediated inflammation, oxidative stress, and hypoxia Article Snippet: .. Article in Press Scientific Reports https://doi.org/10.1038/s41598-026-71415-3 Total protein concentration was determined using the bicinchoninic acid assay with the Pierce |
