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bca protein array kit  (Thermo Fisher)


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    Thermo Fisher bca protein array kit
    Bca Protein Array Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bca+protein+array+kit/BCA+Protein+Assay+Kit/pm41390720-163-24-28
    Average 99 stars, based on 1 article reviews
    bca protein array kit - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Bicinchoninic Acid Protein Assay:

    Article Title: Countering postoperative immune suppression with a self-assembling dendritic cell nanovaccine
    Article Snippet: .. Materials : Pierce bicinchoninic acid (BCA) protein assay kit was purchased from Thermo Scientific (USA). .. Dulbecco's modified Eagle medium (DMEM), Roswell Park Memorial Institute (RPMI)-1640 medium, fetal bovine serum (FBS), trypsin, and penicillin/streptomycin were obtained from Gibco (USA).

    BIA-KA:

    Article Title: Kinase-independent signaling by PIM1 promotes drug resistance by increasing mitophagy and reducing oxidative stress.
    Article Snippet: .. Protein quantification was determined with Pierce BCA Protein Assay Kit (Thermo Scientific, #23225) before prepared in 50 mM dithiothreitol (VWR, #0281-5G) and NuPAGE LDS Sample Buffer (4X) (Invitrogen, #NP0008). ..

    Article Title: Polymeric alpha-1 antitrypsin perturbs Paneth cell proteostasis and exacerbates intestinal inflammation.
    Article Snippet: .. Coimmunoprecipitation assay Intestinal crypt lysates were prepared in cold RIPA lysis buffer (50 mM Tris-HCl, 150mM NaCl, 1% Triton X-100, 1 mM EDTA, 0.1% SDS, pH 7.4) in the presence of protease and phosphatase inhibitors, incubated for 30 min on ice, and centrifuged at 16,000 rcf for 15 min. Supernatants were collected, and the protein concentration was determined with a Pierce BCA protein assay kit. ..

    Article Title: RhoA through its various effectors mediate mitochondrial fragmentation in polycystic kidney disease.
    Article Snippet: .. Cell lysis buffer contained 30 mM HEPES, pH 7.4, 100 mM NaCl, 1% Triton X-100, 20 mM NaF, 1 mM EGTA, 1 mM Na3V_O4 (Cell Signaling), 1 mM PMSF (Bioshop), protease inhibitors (Roche), phosphatase inhibitors (PhosSTOP, Roche); and protein content was measured by BCA Protein Assay Kit (ThermoFisher Scientific). ..

    Article Title: An epigenetic program dictates MYC amplification dynamics that promote transient, extrachromosomal, and inherited genomic events.
    Article Snippet: .. The resulting pellet was lysed in IPH buffer (50 mM Tris-HCl, pH 8.0; 150 mM NaCl; 5 mM EDTA; 0.5% NP-40; 10% glycerol; with protease and phosphatase inhibitors) and sonicated with the Qsonica Q800R2 system (Qsonica) at 70% amplitude 30sec on 30sec off setting for 20 min. Lysates were clarified by centrifugation at maximum speed for 10 min at 4 ◦C and protein concentrations determined using the Pierce BCA Protein Assay Kit (Thermo Scientific). .. For each immunoprecipitation, 500 μg of protein lysate was incubated overnight at 4 ◦C on a rotator in 1 mL IPH buffer with 25 μL Dynabeads Protein A (Invitrogen, 10002D), 2 μg anti-KDM4C antibody (NBP49600, Novus Biologicals) or rabbit IgG (negative control), and 100 μg/mL ethidium bromide.

    Article Title: Opisthorchis viverrini infection is associated with kidney injury involving excretory–secretory product-mediated inflammation, oxidative stress, and hypoxia
    Article Snippet: .. Article in Press Scientific Reports https://doi.org/10.1038/s41598-026-71415-3 Total protein concentration was determined using the bicinchoninic acid assay with the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific), according to the manufacturer’s microplate protocol with minor modifications. ..

    Co-Immunoprecipitation Assay:

    Article Title: Polymeric alpha-1 antitrypsin perturbs Paneth cell proteostasis and exacerbates intestinal inflammation.
    Article Snippet: .. Coimmunoprecipitation assay Intestinal crypt lysates were prepared in cold RIPA lysis buffer (50 mM Tris-HCl, 150mM NaCl, 1% Triton X-100, 1 mM EDTA, 0.1% SDS, pH 7.4) in the presence of protease and phosphatase inhibitors, incubated for 30 min on ice, and centrifuged at 16,000 rcf for 15 min. Supernatants were collected, and the protein concentration was determined with a Pierce BCA protein assay kit. ..

    Lysis:

    Article Title: Polymeric alpha-1 antitrypsin perturbs Paneth cell proteostasis and exacerbates intestinal inflammation.
    Article Snippet: .. Coimmunoprecipitation assay Intestinal crypt lysates were prepared in cold RIPA lysis buffer (50 mM Tris-HCl, 150mM NaCl, 1% Triton X-100, 1 mM EDTA, 0.1% SDS, pH 7.4) in the presence of protease and phosphatase inhibitors, incubated for 30 min on ice, and centrifuged at 16,000 rcf for 15 min. Supernatants were collected, and the protein concentration was determined with a Pierce BCA protein assay kit. ..

    Article Title: RhoA through its various effectors mediate mitochondrial fragmentation in polycystic kidney disease.
    Article Snippet: .. Cell lysis buffer contained 30 mM HEPES, pH 7.4, 100 mM NaCl, 1% Triton X-100, 20 mM NaF, 1 mM EGTA, 1 mM Na3V_O4 (Cell Signaling), 1 mM PMSF (Bioshop), protease inhibitors (Roche), phosphatase inhibitors (PhosSTOP, Roche); and protein content was measured by BCA Protein Assay Kit (ThermoFisher Scientific). ..

    Incubation:

    Article Title: Polymeric alpha-1 antitrypsin perturbs Paneth cell proteostasis and exacerbates intestinal inflammation.
    Article Snippet: .. Coimmunoprecipitation assay Intestinal crypt lysates were prepared in cold RIPA lysis buffer (50 mM Tris-HCl, 150mM NaCl, 1% Triton X-100, 1 mM EDTA, 0.1% SDS, pH 7.4) in the presence of protease and phosphatase inhibitors, incubated for 30 min on ice, and centrifuged at 16,000 rcf for 15 min. Supernatants were collected, and the protein concentration was determined with a Pierce BCA protein assay kit. ..

    Protein Concentration:

    Article Title: Polymeric alpha-1 antitrypsin perturbs Paneth cell proteostasis and exacerbates intestinal inflammation.
    Article Snippet: .. Coimmunoprecipitation assay Intestinal crypt lysates were prepared in cold RIPA lysis buffer (50 mM Tris-HCl, 150mM NaCl, 1% Triton X-100, 1 mM EDTA, 0.1% SDS, pH 7.4) in the presence of protease and phosphatase inhibitors, incubated for 30 min on ice, and centrifuged at 16,000 rcf for 15 min. Supernatants were collected, and the protein concentration was determined with a Pierce BCA protein assay kit. ..

    Article Title: Opisthorchis viverrini infection is associated with kidney injury involving excretory–secretory product-mediated inflammation, oxidative stress, and hypoxia
    Article Snippet: .. Article in Press Scientific Reports https://doi.org/10.1038/s41598-026-71415-3 Total protein concentration was determined using the bicinchoninic acid assay with the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific), according to the manufacturer’s microplate protocol with minor modifications. ..

    other:

    Article Title: Vitamin D alleviates intracerebral hemorrhage symptoms by modulating pro-inflammatory/anti-inflammatory phenotypic transformation of microglia through P2X7R activation of NLRP3
    Article Snippet: The protein concentration was determined using a BCA (Bicinchoninic Acid) protein assay kit (Thermo Fisher Scientific, MA, USA).

    Article Title: Transmembrane PhoxID: photoproximity labelling across the plasma membrane in vivo
    Article Snippet: Following centrifugation (17,730 g, 10min, 4 °C), the protein concentrations of the supernatant were analysed by BCA assay (Pierce), and the normalized lysates were mixed with a quarter volume of 5 × sample buffer (pH 6.8, 312.5mM Tris–HCl, 25% sucrose, 10% SDS, 0.025% bromophenol blue) containing 250mM dithiothreitol (DTT) and vortexed for 1 h at room temperature.

    Cell-Signaling:

    Article Title: RhoA through its various effectors mediate mitochondrial fragmentation in polycystic kidney disease.
    Article Snippet: .. Cell lysis buffer contained 30 mM HEPES, pH 7.4, 100 mM NaCl, 1% Triton X-100, 20 mM NaF, 1 mM EGTA, 1 mM Na3V_O4 (Cell Signaling), 1 mM PMSF (Bioshop), protease inhibitors (Roche), phosphatase inhibitors (PhosSTOP, Roche); and protein content was measured by BCA Protein Assay Kit (ThermoFisher Scientific). ..

    Sonication:

    Article Title: An epigenetic program dictates MYC amplification dynamics that promote transient, extrachromosomal, and inherited genomic events.
    Article Snippet: .. The resulting pellet was lysed in IPH buffer (50 mM Tris-HCl, pH 8.0; 150 mM NaCl; 5 mM EDTA; 0.5% NP-40; 10% glycerol; with protease and phosphatase inhibitors) and sonicated with the Qsonica Q800R2 system (Qsonica) at 70% amplitude 30sec on 30sec off setting for 20 min. Lysates were clarified by centrifugation at maximum speed for 10 min at 4 ◦C and protein concentrations determined using the Pierce BCA Protein Assay Kit (Thermo Scientific). .. For each immunoprecipitation, 500 μg of protein lysate was incubated overnight at 4 ◦C on a rotator in 1 mL IPH buffer with 25 μL Dynabeads Protein A (Invitrogen, 10002D), 2 μg anti-KDM4C antibody (NBP49600, Novus Biologicals) or rabbit IgG (negative control), and 100 μg/mL ethidium bromide.

    Centrifugation:

    Article Title: An epigenetic program dictates MYC amplification dynamics that promote transient, extrachromosomal, and inherited genomic events.
    Article Snippet: .. The resulting pellet was lysed in IPH buffer (50 mM Tris-HCl, pH 8.0; 150 mM NaCl; 5 mM EDTA; 0.5% NP-40; 10% glycerol; with protease and phosphatase inhibitors) and sonicated with the Qsonica Q800R2 system (Qsonica) at 70% amplitude 30sec on 30sec off setting for 20 min. Lysates were clarified by centrifugation at maximum speed for 10 min at 4 ◦C and protein concentrations determined using the Pierce BCA Protein Assay Kit (Thermo Scientific). .. For each immunoprecipitation, 500 μg of protein lysate was incubated overnight at 4 ◦C on a rotator in 1 mL IPH buffer with 25 μL Dynabeads Protein A (Invitrogen, 10002D), 2 μg anti-KDM4C antibody (NBP49600, Novus Biologicals) or rabbit IgG (negative control), and 100 μg/mL ethidium bromide.

    Acid Assay:

    Article Title: Opisthorchis viverrini infection is associated with kidney injury involving excretory–secretory product-mediated inflammation, oxidative stress, and hypoxia
    Article Snippet: .. Article in Press Scientific Reports https://doi.org/10.1038/s41598-026-71415-3 Total protein concentration was determined using the bicinchoninic acid assay with the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific), according to the manufacturer’s microplate protocol with minor modifications. ..



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    Key resources table
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    Image Search Results


    Key resources table

    Journal: iScience

    Article Title: Adiponectin-mediated promotion of CD44 suppresses diabetic vascular inflammatory effects

    doi: 10.1016/j.isci.2023.106428

    Figure Lengend Snippet: Key resources table

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-reptin Cell Signaling Technology Cat#12668S; RRID: AB_2797987 Rabbit monoclonal anti-IgG Cell Signaling Technology Cat#14708S; RRID: AB_2798581 Rabbit monoclonal anti-APPL1 Cell Signaling Technology Cat#3858S; RRID: AB_2056989 Rabbit monoclonal anti-Histone 2A Cell Signaling Technology Cat#7631S; RRID: AB_10860771 Rabbit monoclonal anti-GAPDH Cell Signaling Technology Cat#5174S; RRID: AB_10622025 Rabbit monoclonal anti-β-catenin Cell Signaling Technology Cat#8480S; RRID: AB_11127855 Rabbit monoclonal anti-Non-β-catenin Cell Signaling Technology Cat#8814S; RRID: AB_11127203 NF-κB Pathway Antibody Sampler Kit Cell Signaling Technology Cat#9936T; RRID: AB_561197 Rabbit monoclonal anti-CD44 Cell Signaling Technology Cat#37259S; RRID: AB_2750879 Rabbit monoclonal anti-ICAM-1 Cell Signaling Technology Cat#67836S; RRID: AB_2799738 Biological samples Serum of Healthy control subjects and Diabetes patients Anzhen Hospital, Capital Medical University, Beijing, China https://anzhen.org/ Chemicals, peptides, and recombinant proteins Recombinant Human gAcrp30/Adipolean Peprotech.inc CAS:25-450-21 Recombinant CD44 protein ®Sangon Biotech CAS:D622619 Critical commercial assays BCA Protein Array Kit Thermo Fisher Scientific, Inc. CAS:23227 Qproteome Cell Compartment Kit CAS:37502 The Transcription Factor Activation Profiling Plate Array II Signosis, Sunnyvale, CA CAS: FA-1002 RT2 ProfilerTM PCR Array Human Transcription Factors Qiagen, USA GeneGlobe ID-PAHS-075ZA-24; CAS: 330231 ELISA Kit for Adiponectin (ADPN) Cloud-Clone Corp CAS: SEA605Hu ELISA Kit for CD44 Cloud-Clone Corp CAS: SEA670Hu EZ-Link Sulfo-NHS-LC-Biotinylation kit Thermo Fisher Scientific, Inc. CAS:21435 Deposited data Raw and analyzed data This paper GEO: GSE217607 Experimental models: Cell lines Human umbilical vein endothelial cells (HUVECs): 4201HUM-CCTCC00635 NICR http://cellresource.cn/fdetail.aspx?id=5307/ Experimental models: Organisms/strains Mouse: APPL1 −/− , 8 weeks’s old BRL Medicine Inc. N/A Mouse: APN −/− , 8 weeks’s old Gift N/A Oligonucleotides siRNA targeting sequence: APPL1 #1: UCUCACCUGACUUCGAAACU This paper N/A siRNA targeting sequence: CD44 #1: GAACAAGGAGUCGUCAGAAACUCCA This paper N/A Primers, see Table S2 This paper N/A Software and algorithms GraphPad Prism 8.0 GraphPad Software Inc., San Diego, CA https://www.graphpad.com/ SPSS Statistics 25.0 SPSS Inc., Chicago, IL https://www.ibm.com/ Open in a separate window Key resources table .

    Techniques: Recombinant, Protein Array, Activation Assay, Enzyme-linked Immunosorbent Assay, Sequencing, Software