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Servicebio Inc bca protein assay kit
Bca Protein Assay Kit, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bca+kit/assay+bca+kit+protein/pmc13145395-112-13-18
Average 86 stars, based on 1 article reviews
bca protein assay kit - by Bioz Stars, 2026-11
86/100 stars

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Article Title: Exercise-derived exosomal miR-151-3p: An innovative anti-inflammatory and antioxidant therapeutic for spinal cord injury
Article Snippet: Servicebio (Wuhan, China) provided the BCA protein assay (G2026), CD9 ( GB155697 ), CD63 ( GB115712 ), CD81 ( GB111073 ), Calnexin ( GB151369 ), GM130 ( GB150094 ) and tumor susceptibility gene 101 (TSG101) (GB11618).

Article Title: TRIM21-mediated ubiquitination of PARP1 regulated by the PI3K/AKT-STAT5A axis suppresses small cell lung cancer.
Article Snippet: The assays were performed using a commercially available His-tag Protein Purification Kit (Beyotime, #P2229S) according to the manufacturer’s instructions.

Centrifugation:

Article Title: Activation of the Reverse Cholesterol Transport Pathway: A Strategy to Ameliorate Lipid Metabolic Dyshomeostasis in the Microenvironment Following Spinal Cord Injury.
Article Snippet: Background: Spinal cord injury (SCI) causes massive release of myelin‐derived lipids, driving macrophage‐to‐foam‐cell transformation that sustains inflammation and impedes repair.. Although reverse cholesterol transport (RCT) mediates lipid efflux, its functional dynamics within the SCI microenvironment remain unclear.. Methods: Using a mouse T10 contusion model, we examined key RCT components (ABCA1, ABCG1, and APOE) by qRT‐PCR, Western blot, and immunofluorescence.

Article Title: TRIM21-mediated ubiquitination of PARP1 regulated by the PI3K/AKT-STAT5A axis suppresses small cell lung cancer.
Article Snippet: Total protein extracts were prepared using a lysis buffer composed of 150 mM NaCl, 50 mM Tris-HCl (pH 8.0), 1% Triton X-100, and 1 mM EDTA, supplemented with a cocktail of protease and phosphatase inhibitors (Roche). .. After clarification by centrifugation, the concentration of the total protein was quantified using a bicinchoninic acid assay kit (Servicebio, #G2026). .. An aliquot of 20 μg of protein was subjected to separation by SDS-PAGE and subsequently transferred onto an Immobilon-P PVDF membrane (Millipore, #IPVH00010).

BIA-KA:

Article Title: Activation of the Reverse Cholesterol Transport Pathway: A Strategy to Ameliorate Lipid Metabolic Dyshomeostasis in the Microenvironment Following Spinal Cord Injury.
Article Snippet: Background: Spinal cord injury (SCI) causes massive release of myelin‐derived lipids, driving macrophage‐to‐foam‐cell transformation that sustains inflammation and impedes repair.. Although reverse cholesterol transport (RCT) mediates lipid efflux, its functional dynamics within the SCI microenvironment remain unclear.. Methods: Using a mouse T10 contusion model, we examined key RCT components (ABCA1, ABCG1, and APOE) by qRT‐PCR, Western blot, and immunofluorescence.

Article Title: Sleeve gastrectomy improves cognition by enhancing central ERK/CREB/BDNF pathway through increased GIP secretion
Article Snippet: GLP-1 ELISA Kit , Jianglai Biosciences , Cat# JL11122. .. BCA Protein Quantification Kit , Servicebio , Cat# G2026-200t. ..

Luciferase:

Article Title: The miR-16-1-3p suppresses proliferation and invasiveness via the MDM2-p53 axis in TGF-β1 signaling in osteosarcoma
Article Snippet: For the functional assay, 300 ng of the reporter plasmid were co-transfected with concentration of 50 nM hsa-miR-16-1 mimics using Lipofectamine 3000 (Thermo Fisher Scientific), according to the manufacturer’s instructions. .. After 24 h, luciferase activity was measured using the Dual-Lumi Luciferase Reporter Gene Assay Kit (Servicebio, China) and quantified with a ClarioStar spectrophotometer (BMG Labtech). ..

Activity Assay:

Article Title: The miR-16-1-3p suppresses proliferation and invasiveness via the MDM2-p53 axis in TGF-β1 signaling in osteosarcoma
Article Snippet: For the functional assay, 300 ng of the reporter plasmid were co-transfected with concentration of 50 nM hsa-miR-16-1 mimics using Lipofectamine 3000 (Thermo Fisher Scientific), according to the manufacturer’s instructions. .. After 24 h, luciferase activity was measured using the Dual-Lumi Luciferase Reporter Gene Assay Kit (Servicebio, China) and quantified with a ClarioStar spectrophotometer (BMG Labtech). ..

Reporter Gene Assay:

Article Title: The miR-16-1-3p suppresses proliferation and invasiveness via the MDM2-p53 axis in TGF-β1 signaling in osteosarcoma
Article Snippet: For the functional assay, 300 ng of the reporter plasmid were co-transfected with concentration of 50 nM hsa-miR-16-1 mimics using Lipofectamine 3000 (Thermo Fisher Scientific), according to the manufacturer’s instructions. .. After 24 h, luciferase activity was measured using the Dual-Lumi Luciferase Reporter Gene Assay Kit (Servicebio, China) and quantified with a ClarioStar spectrophotometer (BMG Labtech). ..

Spectrophotometry:

Article Title: The miR-16-1-3p suppresses proliferation and invasiveness via the MDM2-p53 axis in TGF-β1 signaling in osteosarcoma
Article Snippet: For the functional assay, 300 ng of the reporter plasmid were co-transfected with concentration of 50 nM hsa-miR-16-1 mimics using Lipofectamine 3000 (Thermo Fisher Scientific), according to the manufacturer’s instructions. .. After 24 h, luciferase activity was measured using the Dual-Lumi Luciferase Reporter Gene Assay Kit (Servicebio, China) and quantified with a ClarioStar spectrophotometer (BMG Labtech). ..

Clarification Assay:

Article Title: TRIM21-mediated ubiquitination of PARP1 regulated by the PI3K/AKT-STAT5A axis suppresses small cell lung cancer.
Article Snippet: Total protein extracts were prepared using a lysis buffer composed of 150 mM NaCl, 50 mM Tris-HCl (pH 8.0), 1% Triton X-100, and 1 mM EDTA, supplemented with a cocktail of protease and phosphatase inhibitors (Roche). .. After clarification by centrifugation, the concentration of the total protein was quantified using a bicinchoninic acid assay kit (Servicebio, #G2026). .. An aliquot of 20 μg of protein was subjected to separation by SDS-PAGE and subsequently transferred onto an Immobilon-P PVDF membrane (Millipore, #IPVH00010).

Concentration Assay:

Article Title: TRIM21-mediated ubiquitination of PARP1 regulated by the PI3K/AKT-STAT5A axis suppresses small cell lung cancer.
Article Snippet: Total protein extracts were prepared using a lysis buffer composed of 150 mM NaCl, 50 mM Tris-HCl (pH 8.0), 1% Triton X-100, and 1 mM EDTA, supplemented with a cocktail of protease and phosphatase inhibitors (Roche). .. After clarification by centrifugation, the concentration of the total protein was quantified using a bicinchoninic acid assay kit (Servicebio, #G2026). .. An aliquot of 20 μg of protein was subjected to separation by SDS-PAGE and subsequently transferred onto an Immobilon-P PVDF membrane (Millipore, #IPVH00010).

Acid Assay:

Article Title: TRIM21-mediated ubiquitination of PARP1 regulated by the PI3K/AKT-STAT5A axis suppresses small cell lung cancer.
Article Snippet: Total protein extracts were prepared using a lysis buffer composed of 150 mM NaCl, 50 mM Tris-HCl (pH 8.0), 1% Triton X-100, and 1 mM EDTA, supplemented with a cocktail of protease and phosphatase inhibitors (Roche). .. After clarification by centrifugation, the concentration of the total protein was quantified using a bicinchoninic acid assay kit (Servicebio, #G2026). .. An aliquot of 20 μg of protein was subjected to separation by SDS-PAGE and subsequently transferred onto an Immobilon-P PVDF membrane (Millipore, #IPVH00010).

TUNEL Assay:

Article Title: Targeting Rap1-YAP1 mechanosignaling for ameliorating acute IOP elevation-induced trabecular meshwork dysfunction
Article Snippet: Active Rap1 Detection Kit , Cell Signaling Technology , Cat# 8818; RRID: AB_3675293. .. TUNEL kit , Servicebio , G1504. .. SuperSignal West Femto Maximum Sensitivity Substrate , Thermo Fisher Scientific , 34096.

Bicinchoninic Acid Protein Assay:

Article Title: Long-term aerobic exercise enhances circulating exosomal miR-214-3p to promote endothelial progenitor cell-mediated repair of endothelial damage induced by obesity
Article Snippet: RIPA lysate (89900; Thermo Fisher Scientific, Waltham, MA, USA) was employed for the extraction of total cellular proteins from the tested cells. .. The supernatant was collected, and the total protein content was determined using a BCA protein assay kit (G2026-200T; Servicebio). ..



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