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basic nucleofector kit for primary mammalian neurons v4xp-3032  (Lonza)


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    Structured Review

    Lonza basic nucleofector kit for primary mammalian neurons v4xp-3032

    Basic Nucleofector Kit For Primary Mammalian Neurons V4xp 3032, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/basic+primary+neuron+nucleofector+kit/4d+nucleofector/pmc10947185-11-10-8
    Average 90 stars, based on 1 article reviews
    basic nucleofector kit for primary mammalian neurons v4xp-3032 - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Compartment-specific regulation of Na V 1.7 in sensory neurons after acute exposure to TNF-α"

    Article Title: Compartment-specific regulation of Na V 1.7 in sensory neurons after acute exposure to TNF-α

    Journal: Cell reports

    doi: 10.1016/j.celrep.2024.113685


    Figure Legend Snippet:

    Techniques Used: Recombinant, Transfection, Enzyme-linked Immunosorbent Assay, Mutagenesis, Software, Transferring, Inverted Microscopy, Microscopy

    Related Articles

    other:

    Article Title: A rapid and robust protocol for generating loss-of-function alleles in pluripotent stem cells
    Article Snippet: P3 Primary Cell 4D-Nucleofector X kit L , Lonza , V4XP-3024.

    Article Title: Genome sequencing reveals CCDC88A variants in malformations of cortical development and immune dysfunction
    Article Snippet: Fibroblasts were electroporated on 4D Nucleofector ® X Unit (Lonza) with the ‘DT-130’ electroporation program.

    Article Title: A rapid and robust protocol for generating loss-of-function alleles in pluripotent stem cells
    Article Snippet: 4D-Nucleofector X unit , Lonza , AAF-1003X.

    Article Title: Genome sequencing reveals CCDC88A variants in malformations of cortical development and immune dysfunction
    Article Snippet: They were resuspended in an electroporation buffer of P2 Primary Cell 4D-Nucleofector ® X Kit L (#V4XP-2024; Lonza, Basel Switzerland) at a density of 1.5 million fibroblasts/100 μl.

    Retroviral:

    Article Title: Methods for selective expansion of gamma delta t-cell populations and compositions thereof
    Article Snippet: .. Retroviral supernatants are generated by co-transfecting 293 cells that stably expressed MMLV gag and pol proteins with each MSGV1 TCR vector and a vector encoding the endogenous virus retroviral envelope protein using Lipofectamine 2000 (Invitrogen) as described previously or by electroporation using the Nucleofector (Lonza). ..

    Generated:

    Article Title: Methods for selective expansion of gamma delta t-cell populations and compositions thereof
    Article Snippet: .. Retroviral supernatants are generated by co-transfecting 293 cells that stably expressed MMLV gag and pol proteins with each MSGV1 TCR vector and a vector encoding the endogenous virus retroviral envelope protein using Lipofectamine 2000 (Invitrogen) as described previously or by electroporation using the Nucleofector (Lonza). ..

    Stable Transfection:

    Article Title: Methods for selective expansion of gamma delta t-cell populations and compositions thereof
    Article Snippet: .. Retroviral supernatants are generated by co-transfecting 293 cells that stably expressed MMLV gag and pol proteins with each MSGV1 TCR vector and a vector encoding the endogenous virus retroviral envelope protein using Lipofectamine 2000 (Invitrogen) as described previously or by electroporation using the Nucleofector (Lonza). ..

    Virus:

    Article Title: Methods for selective expansion of gamma delta t-cell populations and compositions thereof
    Article Snippet: .. Retroviral supernatants are generated by co-transfecting 293 cells that stably expressed MMLV gag and pol proteins with each MSGV1 TCR vector and a vector encoding the endogenous virus retroviral envelope protein using Lipofectamine 2000 (Invitrogen) as described previously or by electroporation using the Nucleofector (Lonza). ..

    Electroporation:

    Article Title: Methods for selective expansion of gamma delta t-cell populations and compositions thereof
    Article Snippet: .. Retroviral supernatants are generated by co-transfecting 293 cells that stably expressed MMLV gag and pol proteins with each MSGV1 TCR vector and a vector encoding the endogenous virus retroviral envelope protein using Lipofectamine 2000 (Invitrogen) as described previously or by electroporation using the Nucleofector (Lonza). ..

    Incubation:

    Article Title: CD56 on intratumoral NK cells: orchestrating NK cell-mediated anti-tumor effects in bladder cancer
    Article Snippet: Briefly, 4 μg of the plasmid was added to 200 μL of the BCa cell suspension before transferring to an electroporation cuvette (Fisher Scientific). .. Nucleofection was performed using the Amaxa Nucleofector II (Lonza) on the T-030 setting, then immediately transferred to warmed cDMEM-10 media and plated for 72 h. Nucleofected T24 BCa cells were harvested and centrifuged at 300 g/rcf, RT for 7 min then resuspended at 10 × 10^6 cells/mL in FB and incubated with APC/Cy7 conjugated human anti-CD56 antibody (0.2 μg/100 μL) and FVD at 37°C, 5% CO 2 for 30 min before spinning down and resuspending in FB. .. T24 cells were then sorted using the purity sort setting on the BD FACSymphony S6 SE (Flow Cytometry Core, University of Texas Health San Antonio), collecting FVD - GFP + CD56 - cells.



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    Image Search Results


    Journal: Cell reports

    Article Title: Compartment-specific regulation of Na V 1.7 in sensory neurons after acute exposure to TNF-α

    doi: 10.1016/j.celrep.2024.113685

    Figure Lengend Snippet:

    Article Snippet: Basic Nucleofector Kit for Primary Mammalian Neurons , Lonza , V4XP-3032.

    Techniques: Recombinant, Transfection, Enzyme-linked Immunosorbent Assay, Mutagenesis, Software, Transferring, Inverted Microscopy, Microscopy