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amaxa™ basic glial cells nucleofector™ solution  (Lonza)


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    Structured Review

    Lonza amaxa™ basic glial cells nucleofector™ solution
    Amaxa™ Basic Glial Cells Nucleofector™ Solution, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/basic+nucleofector+tm+kit/basic+glial+cells+nucleofector+kit/pmc06133749-74-5-10
    Average 90 stars, based on 1 article reviews
    amaxa™ basic glial cells nucleofector™ solution - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Control:

    Article Title: Neurotoxic reactive astrocytes induce cell death via saturated lipids
    Article Snippet: Visualization and imaging of blots was performed using a Konica Minolta SRX-101a with CL-XPosure Film (Thermo, 34090). .. siRNAs against rat transcripts were acquired from Dharmacon and included: ON-TARGETplus Non-targeting Control Pool, ON-TARGETplus SMARTpool Scd siRNA, and ON-TARGETplus SMARTpool Insig1 siRNA. siRNAs were transfected into cultured rat OPCs using the basic glial cells nucleofector kit (Lonza) using a Nucleofector 2b Device (Lonza) according to manufacturer’s protocol. ..

    Transfection:

    Article Title: Neurotoxic reactive astrocytes induce cell death via saturated lipids
    Article Snippet: Visualization and imaging of blots was performed using a Konica Minolta SRX-101a with CL-XPosure Film (Thermo, 34090). .. siRNAs against rat transcripts were acquired from Dharmacon and included: ON-TARGETplus Non-targeting Control Pool, ON-TARGETplus SMARTpool Scd siRNA, and ON-TARGETplus SMARTpool Insig1 siRNA. siRNAs were transfected into cultured rat OPCs using the basic glial cells nucleofector kit (Lonza) using a Nucleofector 2b Device (Lonza) according to manufacturer’s protocol. ..

    Article Title: Neurotoxic reactive astrocytes induce cell death via saturated lipids.
    Article Snippet: Astrocytes regulate the response of the central nervous system to disease and injury and have been hypothesized to actively kill neurons in neurodegenerative disease.. Here we report an approach to isolate one component of the long-sought astrocyte-derived toxic factor.. Notably, instead of a protein, saturated lipids contained in APOE and APOJ lipoparticles mediate astrocyte-induced toxicity.

    Article Title: PTEN inhibits AMPK to control collective migration
    Article Snippet: .. Astrocytes were transfected with siRNAs (1–5 nM) or plasmids (5 μg) using a Nucleofector machine (Lonza) and the appropriate Lonza glial cell nucleofector solution. .. Transfected cells were then plated on appropriate supports previously coated with poly-L-Ornythin (Sigma) and experiments were performed 4 days post-transfection, when optimal protein silencing or expression was observed.

    Article Title: C21orf91 Regulates Oligodendroglial Precursor Cell Fate—A Switch in the Glial Lineage?
    Article Snippet: Briefly, the complete coding sequence of the rat ortholog C21orf91 ( RGD1563888 ) was inserted into the company’s pV22 vector, which is an equivalent vector to pHTN (Promega France; eucaryotic expression vector containing a HaloTag sequence) with slightly different polylinkers. .. OPCs were transfected via electroporation using the basic glia nucleofector kit and a nucleofector II device (both Lonza, Basel, Switzerland). ..

    Cell Culture:

    Article Title: Neurotoxic reactive astrocytes induce cell death via saturated lipids
    Article Snippet: Visualization and imaging of blots was performed using a Konica Minolta SRX-101a with CL-XPosure Film (Thermo, 34090). .. siRNAs against rat transcripts were acquired from Dharmacon and included: ON-TARGETplus Non-targeting Control Pool, ON-TARGETplus SMARTpool Scd siRNA, and ON-TARGETplus SMARTpool Insig1 siRNA. siRNAs were transfected into cultured rat OPCs using the basic glial cells nucleofector kit (Lonza) using a Nucleofector 2b Device (Lonza) according to manufacturer’s protocol. ..

    Article Title: Neurotoxic reactive astrocytes induce cell death via saturated lipids.
    Article Snippet: Astrocytes regulate the response of the central nervous system to disease and injury and have been hypothesized to actively kill neurons in neurodegenerative disease.. Here we report an approach to isolate one component of the long-sought astrocyte-derived toxic factor.. Notably, instead of a protein, saturated lipids contained in APOE and APOJ lipoparticles mediate astrocyte-induced toxicity.

    Electroporation:

    Article Title: TMEM59 interacts with TREM2 and modulates TREM2-dependent microglial activities
    Article Snippet: For BV2 cells, plasmids were transfected by electroporation using an Amaxa Nucleofector (Lonza, Basel, Switzerland) and Amaxa_Cell Line Nucleofector_Kit T (Lonza), following the manufacturer’s instruction. .. Downregulation of Tmem59 was carried out by electroporation of gene-specific siRNAs using an Amaxa Nucleofector and Amaxa_Cell Line Nucleofector_Kit T (for BV2 cells) or Basic Glial Cells NucleofectorTM Kit (Lonza, for primary microglia), following the manufacturer’s instructions. ..

    Article Title: C21orf91 Regulates Oligodendroglial Precursor Cell Fate—A Switch in the Glial Lineage?
    Article Snippet: Briefly, the complete coding sequence of the rat ortholog C21orf91 ( RGD1563888 ) was inserted into the company’s pV22 vector, which is an equivalent vector to pHTN (Promega France; eucaryotic expression vector containing a HaloTag sequence) with slightly different polylinkers. .. OPCs were transfected via electroporation using the basic glia nucleofector kit and a nucleofector II device (both Lonza, Basel, Switzerland). ..



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    Image Search Results


    Journal: STAR Protocols

    Article Title: Protocol for electron microscopy ultrastructural localization of the fusogenic lipid phosphatidic acid on plasma membrane sheets from chromaffin cells

    doi: 10.1016/j.xpro.2021.100464

    Figure Lengend Snippet:

    Article Snippet: Basic Nucleofector TM Kit for Primary Mammalian Neurons , Lonza , Cat#VVPI-1003.

    Techniques: Recombinant, Clinical Proteomics, Modification, Electron Microscopy, Plasmid Preparation, Software