basic parasite nucleofector kit 1 (Lonza)

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Lonza basic parasite nucleofector kit 1
Basic Parasite Nucleofector Kit 1, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/basic+nucleofector+tm+kit/basic+parasite+nucleofector+kit+2/pmc05907651-245-21-26
Average 90 stars, based on 1 article reviews

basic parasite nucleofector kit 1 - by Bioz Stars, 2026-09

90/100 stars

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Centrifugation:

Article Title: A multistage Plasmodium CRL4 WIG1 ubiquitin ligase is critical for the formation of functional microtubule organization centers in microgametocytes.
Article Snippet: .. Parasites were harvested from the interface and collected by centrifugation at 500 × g for 3 min, resuspended in 25 μL Basic Parasite Nucleofector solution (Lonza) and added to 10–20 μg DNA dissolved in 10 μL H2O. .. Cells were electroporated using the FI-115 program of the Amaxa Nucleofector 4D.

Article Title: A multistage Plasmodium CRL4 WIG1 ubiquitin ligase is critical for the formation of functional microtubule organization centers in microgametocytes
Article Snippet: .. Parasites were harvested from the interface and collected by centrifugation at 500 × g for 3 min, resuspended in 25 μL Basic Parasite Nucleofector solution (Lonza) and added to 10–20 μg DNA dissolved in 10 μL H 2 O. .. Cells were electroporated using the FI-115 program of the Amaxa Nucleofector 4D.

Transfection:

Article Title: Multi-drug tolerance in Leishmania persister-like cells
Article Snippet: .. A quantity of 2 × 107 promastigotes was transfected with 100 ng of the linearized DNA fragment containing EGFP, using the Basic Parasite Nucleofector kit 1 (VMI 1011, Lonza); program U-033. ..

Article Title: The glycosomal ATP-dependent phosphofructokinase of Trypanosoma brucei operates also in the gluconeogenic direction
Article Snippet: Production of the pLew-FBPase-SAS plasmid and the RNAi FBPase cell lines was described before [ ]. .. To generate the ∆ sbpase /∆ hk / RNAi FBPase and ∆ sbpase /∆ hk /∆ PFK / RNAi FBPase mutants the ∆ sbpase /∆ hk and ∆ sbpase /∆ hk /∆ pfk cell lines were transfected with the Not I-linearized pLew100-FBPase-SAS plasmid in 2-mm electroporation cuvettes with Basic Parasite Nucleofector Kit 2 on the Amaxa Nucleofector device (Lonza), using X-001 program. ..

Article Title: The glycosomal ATP-dependent phosphofructokinase of Trypanosoma brucei operates also in the gluconeogenic direction
Article Snippet: Subsequently, this mixture was combined with 30 μg of Sp Cas9 sourced from IDT (Alt-RTM Sp Cas9 Nuclease 3NLS, Catalog #1074181). .. 5 × 10 5 cells were respectively transfected using Amaxa nucleofector device with Basic Parasite Nucleofector Kit 2 (Lonza) (100 μl) with 1 μg of purified repair cassette (in a final volume of 10 μl) and 30 μg of Cas9 protein preloaded with the mixture of TracrRNA and gRNA. ..

Article Title: Improved base editing and functional screening in Leishmania via co-expression of the AsCas12a ultra variant, a T7 RNA polymerase, and a cytosine base editor
Article Snippet: .. We therefore conducted a side-by-side comparison of the transfection efficiency between the Lonza Nucleofector Basic Parasite kit and the Trypanosoma -optimized buffer (Tb-BSF buffer, see Materials and methods section for further details) , both in combination with an Amaxa Nucleofector 2b electroporator. ..

Article Title: Improved base editing and functional screening in Leishmania via co-expression of the AsCas12a ultra variant, a T7 RNA polymerase, and a cytosine base editor
Article Snippet: Transfections were generally carried out as described in , transfecting ~5 × 10 6 cells in 1× Tb-BSF buffer ( ) using 2 mm cuvettes (BTX) with an Amaxa Nucleofector 2b (Lonza, program X-001). .. For high efficiency transfections, we modified the protocol and compared side-by-side the transfection efficiency of the Basic Parasite Nucleofector Kit (Lonza) and Tb-BSF protocol ( ). .. 1×10 8 or 2.5×10 8 cells were collected, washed once in 1× Tb-BSF buffer, and resuspended in either 100 μl Lonza transfection reagent or 200 μl Tb-BSF buffer.

Virus:

Article Title: A new member of the dynamin superfamily modulates mitochondrial membrane branching in Trypanosoma brucei.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-Ty1 Gift of K. Gull; Bastin et al.74 N/A Rabbit anti-Prohibitin Gift of J. Luke s; Tyc et al.75 N/A Rabbit anti-Enolase Gift of P. Michels; Hannaert et al.37 N/A Rabbit anti-Atom40 Gift of A. Schneider; Mani et al.76 N/A Rabbit anti-Fumarate reductase glycosomal (Frdg) Gift of F. Bringaud; Besteiro et al.77 N/A Mouse anti-HSP60 clone H7 Gift of F. Bringaud; Bringaud et al.78 N/A Rabbit anti-Pyruvate Phosphate DiKinase (PPDK) Gift of F. Bringaud; Bringaud et al.79 N/A Rabbit anti-Threonine dehydrogenase (Tdh) Gift of F. Bringaud; Millerioux et al.35 N/A Rabbit anti-GFP Abcam Catalog #ab290 Mouse anti-HA.11 BioLegend Catalog #901516 Rat anti-HA High Affinity Roche Catalog #11867423001 Goat anti-Mouse-HRP Sigma-Aldrich Catalog #A5278 Goat anti-Rabbit-HRP SeraCare Catalog #074-15-061 Donkey anti-Mouse Alexa fluor 594 Thermofisher Catalog #A-1105 Donkey anti-Mouse Alexa fluor 488 Thermofisher Catalog #A-21202 Donkey anti-Rabbit Alexa fluor 594 Thermofisher Catalog #A-11012 Donkey anti-Rabbit Alexa fluor 488 Thermofisher Catalog #A-21206 Donkey anti-Rat Alexa fluor 594 Thermofisher Catalog #A-21209 Anti-Mousse IgG gold British BioCell International Catalog #EM.GAM10 Bacterial and virus strains XL1-blue Avantor Catalog #AGLS200249 Chemicals, peptides, and recombinant proteins Proteinase K Sigma-Aldrich Catalog #P6556-100MG Digitonin Sigma-Aldrich Catalog #D141-500MG Phenylmethanesulfonyl fluoride (PMSF) Roche Catalog #10837091001 Triton X-100 solution Sigma-Aldrich Catalog #T8787-250ML Rhodamine 123 Sigma-Aldrich Catalog #83702-10MG Hoechst 33342 Sigma-Aldrich Catalog #B2261-25MG DAPI Sigma-Aldrich Catalog #D9542-10MG Paraformaldehyde 32% Electron Microscopy Sciences Catalog #15714 Critical commercial assays Clarity western enhanced-chemiluminescence Bio-Rad Catalog #1705060 NucleoSpin Blood Macherey-Nagel Catalog #740951.250 MitoTracker Green FM Invitrogen Catalog #M7514 Cas9 enzyme Integrated DNA Technologies Catalog #1081059 Basic Parasite Nucleofector Kit 2 Lonza Catalog #VMI-1021 SlowFade Gold antifade reagent Thermofisher Catalog #S 36836 Deposited data Additional data have been deposited in an online data repository (Mendeley) This paper https://doi.org/10.17632/jr3jbf6fp8.1 Oligonucleotides For oligonucleotides used in this study: see Table S2 Integrated DNA Technologies N/A (Continued on next page) Current Biology 35, 1–16.e1–e5, March 24, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pLew100 Wirtz et al.80 N/A pPOTv7 Paterou et al.81 N/A p2T7-177 Wickstead et al.82 N/A pHD1336 Biebinger et al.83 N/A Software and algorithms GraphPad Prism 9 GraphPad https://www.graphpad.com/ Image Lab V6.1 Bio-Rad https://www.bio-rad.com Geneious R11 Biomatters https://www.geneious.com/ Fiji Schindelin et al.84 https://imagej.net/software/fiji/ Metamorph V7 Molecular Devices https://www.moleculardevices.com/ Article

Recombinant:

Electron Microscopy:

Western Blot:

Plasmid Preparation:

Electroporation:

Purification:

Comparison:

Article Title: Improved base editing and functional screening in Leishmania via co-expression of the AsCas12a ultra variant, a T7 RNA polymerase, and a cytosine base editor
Article Snippet: .. We therefore conducted a side-by-side comparison of the transfection efficiency between the Lonza Nucleofector Basic Parasite kit and the Trypanosoma -optimized buffer (Tb-BSF buffer, see Materials and methods section for further details) , both in combination with an Amaxa Nucleofector 2b electroporator. ..

Modification:

Article Title: Improved base editing and functional screening in Leishmania via co-expression of the AsCas12a ultra variant, a T7 RNA polymerase, and a cytosine base editor
Article Snippet: Transfections were generally carried out as described in , transfecting ~5 × 10 6 cells in 1× Tb-BSF buffer ( ) using 2 mm cuvettes (BTX) with an Amaxa Nucleofector 2b (Lonza, program X-001). .. For high efficiency transfections, we modified the protocol and compared side-by-side the transfection efficiency of the Basic Parasite Nucleofector Kit (Lonza) and Tb-BSF protocol ( ). .. 1×10 8 or 2.5×10 8 cells were collected, washed once in 1× Tb-BSF buffer, and resuspended in either 100 μl Lonza transfection reagent or 200 μl Tb-BSF buffer.

Review