Review



basic nucleofector solution for mammalian glial cells with supplements  (Lonza)


Bioz Verified Symbol Lonza is a verified supplier
Bioz Manufacturer Symbol Lonza manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Lonza basic nucleofector solution for mammalian glial cells with supplements
    Basic Nucleofector Solution For Mammalian Glial Cells With Supplements, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/basic+nucleofector+tm+kit/basic+nucleofector+kit+for+primary+mammalian+glial+cells/pmc04016185-171-24-25
    Average 90 stars, based on 1 article reviews
    basic nucleofector solution for mammalian glial cells with supplements - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Negative Control:

    Article Title: Targeted ASO-mediated Atp1a2 knockdown in astrocytes reduces SOD1 aggregation and accelerates disease onset in mutant SOD1 mice
    Article Snippet: Primary murine astrocytes used in this study were generated from the cortices of post-natal day 2 (P2) pups (n = 10–12) from either CD1 wild-type strain (code: 022, #24101187 from Charles River Laboratories) or SOD1*G93A mice as previously described [ , ]. .. Primary murine astrocytes were nucleofected with a non-targeting negative control A (ctrl ASO) or Atp1a2 -specific ASOs at several concentrations using the AmaxaTM Basic NucleofectorTM Kit for Primary Mammalian Glial Cells (Lonza, VPI-1006) or P3 Primary Cell 4D-Nucleofector TM X Kit (Lonza, V4XP-3032) following the manufacturer’s protocol. ..

    Article Title: Targeted ASO-mediated Atp1a2 knockdown in astrocytes reduces SOD1 aggregation and accelerates disease onset in mutant SOD1 mice.
    Article Snippet: Screening of Atp1a2-specific ASOs in primary astrocytes Primary murine astrocytes used in this study were generated from the cortices of post-natal day 2 (P2) pups (n = 10–12) from either CD1 wild-type strain (code: 022, #24101187 from Charles River Laboratories) or SOD1*G93A mice as previously described [23, 24]. .. Primary murine astrocytes were nucleofected with a non-targeting negative control A (ctrl ASO) or Atp1a2-specific ASOs at several concentrations using the AmaxaTM Basic NucleofectorTM Kit for Primary Mammalian Glial Cells (Lonza, VPI-1006) or P3 Primary Cell 4D-NucleofectorTM X Kit (Lonza, V4XP-3032) following the manufacturer’s protocol. ..

    Allele-specific Oligonucleotide:

    Article Title: Targeted ASO-mediated Atp1a2 knockdown in astrocytes reduces SOD1 aggregation and accelerates disease onset in mutant SOD1 mice
    Article Snippet: Primary murine astrocytes used in this study were generated from the cortices of post-natal day 2 (P2) pups (n = 10–12) from either CD1 wild-type strain (code: 022, #24101187 from Charles River Laboratories) or SOD1*G93A mice as previously described [ , ]. .. Primary murine astrocytes were nucleofected with a non-targeting negative control A (ctrl ASO) or Atp1a2 -specific ASOs at several concentrations using the AmaxaTM Basic NucleofectorTM Kit for Primary Mammalian Glial Cells (Lonza, VPI-1006) or P3 Primary Cell 4D-Nucleofector TM X Kit (Lonza, V4XP-3032) following the manufacturer’s protocol. ..

    Article Title: Targeted ASO-mediated Atp1a2 knockdown in astrocytes reduces SOD1 aggregation and accelerates disease onset in mutant SOD1 mice.
    Article Snippet: Screening of Atp1a2-specific ASOs in primary astrocytes Primary murine astrocytes used in this study were generated from the cortices of post-natal day 2 (P2) pups (n = 10–12) from either CD1 wild-type strain (code: 022, #24101187 from Charles River Laboratories) or SOD1*G93A mice as previously described [23, 24]. .. Primary murine astrocytes were nucleofected with a non-targeting negative control A (ctrl ASO) or Atp1a2-specific ASOs at several concentrations using the AmaxaTM Basic NucleofectorTM Kit for Primary Mammalian Glial Cells (Lonza, VPI-1006) or P3 Primary Cell 4D-NucleofectorTM X Kit (Lonza, V4XP-3032) following the manufacturer’s protocol. ..

    Plasmid Preparation:

    Article Title: Astrocyte-Secreted Lcn2 Modulates Dendritic Spine Morphology
    Article Snippet: .. For the experiments, the astrocytic cells were electroporated with 5 μg of pZac_gfa ABC1D _Lcn2_FLAG_SEP plasmid using nucleofection reagents for primary mammalian glial cells, according to the manufacturer’s protocol (AmaxaTM, Lonza, Basel, Switzerland). ..

    Article Title: Astrocyte-Secreted Lcn2 Modulates Dendritic Spine Morphology.
    Article Snippet: .. For the experiments, the astrocytic cells were electroporated with 5 μg of pZac_gfaABC1D_Lcn2_FLAG_SEP plasmid using nucleofection reagents for primary mammalian glial cells, according to the manufacturer’s protocol (AmaxaTM, Lonza, Basel, Switzerland). ..

    Construct:

    Article Title: Astrocytic polyamine transport by ATP13A4 tunes excitatory synaptic transmission
    Article Snippet: .. For ACM production, during the astrocyte passage step (DIV 7), the astrocytes were nucleofected with constructs carrying either a scramble sequence (shCtrl, 5’-GCTCACCACGTGCAATACTAT-3’) or shRNA (shAtp13a4, 5’-GCCCATGAACTTCAAGCTCTA −3’), using the Basic Nucleofector Kit for Primary Mammalian Glial Cells (Lonza, Cat# VPI-1006) according to the manufacturer’s protocol. ..

    Sequencing:

    Article Title: Astrocytic polyamine transport by ATP13A4 tunes excitatory synaptic transmission
    Article Snippet: .. For ACM production, during the astrocyte passage step (DIV 7), the astrocytes were nucleofected with constructs carrying either a scramble sequence (shCtrl, 5’-GCTCACCACGTGCAATACTAT-3’) or shRNA (shAtp13a4, 5’-GCCCATGAACTTCAAGCTCTA −3’), using the Basic Nucleofector Kit for Primary Mammalian Glial Cells (Lonza, Cat# VPI-1006) according to the manufacturer’s protocol. ..

    shRNA:

    Article Title: Astrocytic polyamine transport by ATP13A4 tunes excitatory synaptic transmission
    Article Snippet: .. For ACM production, during the astrocyte passage step (DIV 7), the astrocytes were nucleofected with constructs carrying either a scramble sequence (shCtrl, 5’-GCTCACCACGTGCAATACTAT-3’) or shRNA (shAtp13a4, 5’-GCCCATGAACTTCAAGCTCTA −3’), using the Basic Nucleofector Kit for Primary Mammalian Glial Cells (Lonza, Cat# VPI-1006) according to the manufacturer’s protocol. ..

    Isolation:

    Article Title: The AMPK activator metformin improves recovery from demyelination by shifting oligodendrocyte bioenergetics and accelerating OPC differentiation
    Article Snippet: .. Nucleofection of isolated OPCs was performed with siRNA mixtures to target both AMPKα 1 (Dharmacon, Cat. No. L-091373-02-0005) and AMPKα 2 (Dharmacon, Cat. No. L-100623-02-0005), using the basic nucleofector kit for primary mammalian glial cells (Lonza Biosciences). .. Control cells were transfected with a pool of non-targeting siRNAs (Dharmacon).



    Similar Products

    90
    Lonza amaxa tm basic nucleofector tm kit
    Amaxa Tm Basic Nucleofector Tm Kit, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/basic+nucleofector+tm+kit/4d+nucleofector/bio_rxiv__2024__04__19__590214-44-24-30
    Average 90 stars, based on 1 article reviews
    amaxa tm basic nucleofector tm kit - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Lonza amaxa tm basic nucleofector tm kit primary fibroblasts
    Amaxa Tm Basic Nucleofector Tm Kit Primary Fibroblasts, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/basic+nucleofector+tm+kit/4d+nucleofector/pmc03537537-232-22-29
    Average 90 stars, based on 1 article reviews
    amaxa tm basic nucleofector tm kit primary fibroblasts - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Lonza amaxa tm basic nucleofector tm kit primary mammalian epithelial cells
    Amaxa Tm Basic Nucleofector Tm Kit Primary Mammalian Epithelial Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/basic+nucleofector+tm+kit/4d+nucleofector/pmc08873204-350-48-52
    Average 90 stars, based on 1 article reviews
    amaxa tm basic nucleofector tm kit primary mammalian epithelial cells - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Lonza basic nucleofector tm kit for primary mammalian neurons

    Basic Nucleofector Tm Kit For Primary Mammalian Neurons, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/basic+nucleofector+tm+kit/basic+nucleofector+kit+for+primary+mammalian+neurons/pmc08065343-44-0-9
    Average 90 stars, based on 1 article reviews
    basic nucleofector tm kit for primary mammalian neurons - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Lonza basic nucleofector tm kit for primary mammalian fibroblasts vpi-1002

    Basic Nucleofector Tm Kit For Primary Mammalian Fibroblasts Vpi 1002, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/basic+nucleofector+tm+kit/4d+nucleofector/pmc07072439-67-13-16
    Average 90 stars, based on 1 article reviews
    basic nucleofector tm kit for primary mammalian fibroblasts vpi-1002 - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Lonza basic nucleofector tm kit

    Basic Nucleofector Tm Kit, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/basic+nucleofector+tm+kit/4d+nucleofector/bio_rxiv__605972-339-28-32
    Average 90 stars, based on 1 article reviews
    basic nucleofector tm kit - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Lonza nucleofector tm solution (basic nucleofector tm kit, primary fibroblasts)

    Nucleofector Tm Solution (Basic Nucleofector Tm Kit, Primary Fibroblasts), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/basic+nucleofector+tm+kit/4d+nucleofector/pm29601977-91-13-19
    Average 90 stars, based on 1 article reviews
    nucleofector tm solution (basic nucleofector tm kit, primary fibroblasts) - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Lonza tm basic nucleofector tm primary fibroblast kit

    Tm Basic Nucleofector Tm Primary Fibroblast Kit, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/basic+nucleofector+tm+kit/4d+nucleofector/pmc05801513-233-7-14
    Average 90 stars, based on 1 article reviews
    tm basic nucleofector tm primary fibroblast kit - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    Image Search Results


    Journal: STAR Protocols

    Article Title: Protocol for electron microscopy ultrastructural localization of the fusogenic lipid phosphatidic acid on plasma membrane sheets from chromaffin cells

    doi: 10.1016/j.xpro.2021.100464

    Figure Lengend Snippet:

    Article Snippet: Basic Nucleofector TM Kit for Primary Mammalian Neurons , Lonza , Cat#VVPI-1003.

    Techniques: Recombinant, Clinical Proteomics, Modification, Electron Microscopy, Plasmid Preparation, Software