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plenti pgk gfp blast  (Addgene inc)


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    Structured Review

    Addgene inc plenti pgk gfp blast
    Plenti Pgk Gfp Blast, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/basic+local+alignment+sequence+tool+blast/pLenti+PGK+GFP+Blast+(w510-5)+(Plasmid+%2319069)/pmc10570043-57-24-25
    Average 93 stars, based on 16 article reviews
    plenti pgk gfp blast - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Transduction:

    Article Title: Targeting Glioblastoma Cell State Plasticity for Enhanced Therapeutic Efficacy
    Article Snippet: .. Briefly, GSCs were transduced with pLenti PGK GFP Blast (w510-5) (Addgene) and were cultured under blasticidin selection in NBE medium. ..

    Article Title: Microenvironment-driven dynamic chromatin changes in glioblastoma recapitulate early neural development at single-cell resolution
    Article Snippet: .. Briefly, GSCs were transduced with pLenti PGK GFP Blast (w510–5) (Addgene) and were cultured under blasticidin selection in NBE medium. ..

    Cell Culture:

    Article Title: Targeting Glioblastoma Cell State Plasticity for Enhanced Therapeutic Efficacy
    Article Snippet: .. Briefly, GSCs were transduced with pLenti PGK GFP Blast (w510-5) (Addgene) and were cultured under blasticidin selection in NBE medium. ..

    Article Title: Microenvironment-driven dynamic chromatin changes in glioblastoma recapitulate early neural development at single-cell resolution
    Article Snippet: .. Briefly, GSCs were transduced with pLenti PGK GFP Blast (w510–5) (Addgene) and were cultured under blasticidin selection in NBE medium. ..

    Selection:

    Article Title: Targeting Glioblastoma Cell State Plasticity for Enhanced Therapeutic Efficacy
    Article Snippet: .. Briefly, GSCs were transduced with pLenti PGK GFP Blast (w510-5) (Addgene) and were cultured under blasticidin selection in NBE medium. ..

    Article Title: Microenvironment-driven dynamic chromatin changes in glioblastoma recapitulate early neural development at single-cell resolution
    Article Snippet: .. Briefly, GSCs were transduced with pLenti PGK GFP Blast (w510–5) (Addgene) and were cultured under blasticidin selection in NBE medium. ..

    Sequencing:

    Article Title: Recovery of protein synthesis to assay DNA repair activity in transcribed genes in living cells and tissues
    Article Snippet: To generate U2OS cells stably expressing EGFP-FKBP F36V , a gene fragment containing EGFP - FKBP F36V with nuclear localization signal behind a PGK promoter and BLAST behind an EF1a promoter, flanked by AAVS1 homology sequences for homology directed repair, was generated by Integrated DNA technologies. .. EGFP sequence was derived from pEGFP-N1 (Clontech), FKBP F36V sequence was derived from pLEX_305-C-dTAG (Addgene #91798) ( ) and BLAST sequence was derived from pLenti-PGK-GFP-Blast (Addgene #19069) ( ). .. The gene fragment was cloned into pUC57 (Genscript) and transfected together with Cas9 (IDT) and an sgRNA targeting AAVS1 (GGGGCCACTAGGGACAGGAT; IDT) into U2OS cells.

    Article Title: Recovery of protein synthesis to assay DNA repair activity in transcribed genes in living cells and tissues.
    Article Snippet: For cisplatin induction of DNA damage, ells were exposed for 2 h to 100 M cisplatin (Sigma). ll reagents used in this stud y, with concentra tions and dentifiers, are listed in Supplementary Table S3. lasmid and cell line generation o generate U2OS cells stably expressing EGFPKBP F36V , a gene fragment containing EGFP - FKBP F36V ith nuclear localization signal behind a PGK prooter and BLAST behind an EF1a promoter, flanked y AAVS1 homology sequences for homology directed epair, was generated by Integrated DN A technolo gies. .. GFP sequence was deri v ed from pEGFP-N1 (Clontech), KBP F36V sequence was deri v ed from pLEX 305-C-dTAG Addgene #91798) ( 38 ) and BLAST sequence was deri v ed rom pLenti-PGK-GFP-Blast (Addgene #19069) ( 39 ). he gene fragment was cloned into pUC57 (Genscript) nd transfected together with Cas9 (IDT) and an sgRNA argeting AAVS1 (GGGGCCA CTAGGGA CAGGAT; DT) into U2OS cells. .. Transfection was performed with ipofectamin CRISPRMAX (Thermofisher) according o the manufacturer’s instructions.

    Derivative Assay:

    Article Title: Recovery of protein synthesis to assay DNA repair activity in transcribed genes in living cells and tissues
    Article Snippet: To generate U2OS cells stably expressing EGFP-FKBP F36V , a gene fragment containing EGFP - FKBP F36V with nuclear localization signal behind a PGK promoter and BLAST behind an EF1a promoter, flanked by AAVS1 homology sequences for homology directed repair, was generated by Integrated DNA technologies. .. EGFP sequence was derived from pEGFP-N1 (Clontech), FKBP F36V sequence was derived from pLEX_305-C-dTAG (Addgene #91798) ( ) and BLAST sequence was derived from pLenti-PGK-GFP-Blast (Addgene #19069) ( ). .. The gene fragment was cloned into pUC57 (Genscript) and transfected together with Cas9 (IDT) and an sgRNA targeting AAVS1 (GGGGCCACTAGGGACAGGAT; IDT) into U2OS cells.

    Clone Assay:

    Article Title: Recovery of protein synthesis to assay DNA repair activity in transcribed genes in living cells and tissues.
    Article Snippet: For cisplatin induction of DNA damage, ells were exposed for 2 h to 100 M cisplatin (Sigma). ll reagents used in this stud y, with concentra tions and dentifiers, are listed in Supplementary Table S3. lasmid and cell line generation o generate U2OS cells stably expressing EGFPKBP F36V , a gene fragment containing EGFP - FKBP F36V ith nuclear localization signal behind a PGK prooter and BLAST behind an EF1a promoter, flanked y AAVS1 homology sequences for homology directed epair, was generated by Integrated DN A technolo gies. .. GFP sequence was deri v ed from pEGFP-N1 (Clontech), KBP F36V sequence was deri v ed from pLEX 305-C-dTAG Addgene #91798) ( 38 ) and BLAST sequence was deri v ed rom pLenti-PGK-GFP-Blast (Addgene #19069) ( 39 ). he gene fragment was cloned into pUC57 (Genscript) nd transfected together with Cas9 (IDT) and an sgRNA argeting AAVS1 (GGGGCCA CTAGGGA CAGGAT; DT) into U2OS cells. .. Transfection was performed with ipofectamin CRISPRMAX (Thermofisher) according o the manufacturer’s instructions.

    Transfection:

    Article Title: Recovery of protein synthesis to assay DNA repair activity in transcribed genes in living cells and tissues.
    Article Snippet: For cisplatin induction of DNA damage, ells were exposed for 2 h to 100 M cisplatin (Sigma). ll reagents used in this stud y, with concentra tions and dentifiers, are listed in Supplementary Table S3. lasmid and cell line generation o generate U2OS cells stably expressing EGFPKBP F36V , a gene fragment containing EGFP - FKBP F36V ith nuclear localization signal behind a PGK prooter and BLAST behind an EF1a promoter, flanked y AAVS1 homology sequences for homology directed epair, was generated by Integrated DN A technolo gies. .. GFP sequence was deri v ed from pEGFP-N1 (Clontech), KBP F36V sequence was deri v ed from pLEX 305-C-dTAG Addgene #91798) ( 38 ) and BLAST sequence was deri v ed rom pLenti-PGK-GFP-Blast (Addgene #19069) ( 39 ). he gene fragment was cloned into pUC57 (Genscript) nd transfected together with Cas9 (IDT) and an sgRNA argeting AAVS1 (GGGGCCA CTAGGGA CAGGAT; DT) into U2OS cells. .. Transfection was performed with ipofectamin CRISPRMAX (Thermofisher) according o the manufacturer’s instructions.

    Plasmid Preparation:

    Article Title: Tumor removal limits prostate cancer cell dissemination in bone and osteoblasts induce cancer cell dormancy through focal adhesion kinase
    Article Snippet: .. Plasmid pLenti-GFP-Blast (gift from Dr. Wei Wu, Tsinghua University) was used for general GFP labeling, and pLenti-PGK-Neo-PIP-FUCCI (Fluorescent Ubiquitination-based Cell Cycle Indicator) (Addgene # 118,616) was used for accurate cell cycle phase indicator [ ]. ..

    Labeling:

    Article Title: Tumor removal limits prostate cancer cell dissemination in bone and osteoblasts induce cancer cell dormancy through focal adhesion kinase
    Article Snippet: .. Plasmid pLenti-GFP-Blast (gift from Dr. Wei Wu, Tsinghua University) was used for general GFP labeling, and pLenti-PGK-Neo-PIP-FUCCI (Fluorescent Ubiquitination-based Cell Cycle Indicator) (Addgene # 118,616) was used for accurate cell cycle phase indicator [ ]. ..

    Ubiquitin Proteomics:

    Article Title: Tumor removal limits prostate cancer cell dissemination in bone and osteoblasts induce cancer cell dormancy through focal adhesion kinase
    Article Snippet: .. Plasmid pLenti-GFP-Blast (gift from Dr. Wei Wu, Tsinghua University) was used for general GFP labeling, and pLenti-PGK-Neo-PIP-FUCCI (Fluorescent Ubiquitination-based Cell Cycle Indicator) (Addgene # 118,616) was used for accurate cell cycle phase indicator [ ]. ..



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