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piggybac transposition  (Addgene inc)


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    Structured Review

    Addgene inc piggybac transposition
    Piggybac Transposition, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/basic+local+alignment+sequence+tool+blast/pJB044+(Plasmid+%2322617)/pm39562138-175-23-28
    Average 92 stars, based on 1 article reviews
    piggybac transposition - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Sequencing:

    Article Title: PROSER1 modulates DNA demethylation through dual mechanisms to prevent syndromic developmental malformations.
    Article Snippet: For re-expression of fulllength PROSER1, the mouse Proser1 coding sequence was amplified from cDNA using primers to add a C-terminal 2XFLAG tag and cloned into a Gateway entry vector (Thermo Scientific K240020SP). .. LR recombination with LR Clonase II enzyme mix (Invitrogen 10134992) was then performed to transfer the sequence-verified clone into a destination vector for PiggyBac transposition (PiggyBac-mProser1-FLAG-IRES-Blast, deposited at Addgene 226173). .. Cells were transfected with a 1:1 ratio of PiggyBac and pBase constructs using Lipofectamine 3000 (Invitrogen L3000008), and the pool of cells was selected with 5 μg/mL blasticidin (Sigma 203350) for ∼10 days prior to subcloning by limited dilution and isolation of clonal mESC lines.

    Article Title: PROSER1 modulates DNA demethylation through dual mechanisms to prevent syndromic developmental malformations
    Article Snippet: For re-expression of full-length PROSER1, the mouse Proser1 coding sequence was amplified from cDNA using primers to add a C-terminal 2XFLAG tag and cloned into a Gateway entry vector (Thermo Scientific K240020SP). .. LR recombination with LR Clonase II enzyme mix (Invitrogen 10134992) was then performed to transfer the sequence-verified clone into a destination vector for PiggyBac transposition (PiggyBac-mProser1-FLAG-IRES-Blast, deposited at Addgene 226173). .. Cells were transfected with a 1:1 ratio of PiggyBac and pBase constructs using Lipofectamine 3000 (Invitrogen L3000008), and the pool of cells was selected with 5 μg/mL blasticidin (Sigma 203350) for ∼10 days prior to subcloning by limited dilution and isolation of clonal mESC lines.

    Plasmid Preparation:

    Article Title: PROSER1 modulates DNA demethylation through dual mechanisms to prevent syndromic developmental malformations.
    Article Snippet: For re-expression of fulllength PROSER1, the mouse Proser1 coding sequence was amplified from cDNA using primers to add a C-terminal 2XFLAG tag and cloned into a Gateway entry vector (Thermo Scientific K240020SP). .. LR recombination with LR Clonase II enzyme mix (Invitrogen 10134992) was then performed to transfer the sequence-verified clone into a destination vector for PiggyBac transposition (PiggyBac-mProser1-FLAG-IRES-Blast, deposited at Addgene 226173). .. Cells were transfected with a 1:1 ratio of PiggyBac and pBase constructs using Lipofectamine 3000 (Invitrogen L3000008), and the pool of cells was selected with 5 μg/mL blasticidin (Sigma 203350) for ∼10 days prior to subcloning by limited dilution and isolation of clonal mESC lines.

    Article Title: PROSER1 modulates DNA demethylation through dual mechanisms to prevent syndromic developmental malformations
    Article Snippet: For re-expression of full-length PROSER1, the mouse Proser1 coding sequence was amplified from cDNA using primers to add a C-terminal 2XFLAG tag and cloned into a Gateway entry vector (Thermo Scientific K240020SP). .. LR recombination with LR Clonase II enzyme mix (Invitrogen 10134992) was then performed to transfer the sequence-verified clone into a destination vector for PiggyBac transposition (PiggyBac-mProser1-FLAG-IRES-Blast, deposited at Addgene 226173). .. Cells were transfected with a 1:1 ratio of PiggyBac and pBase constructs using Lipofectamine 3000 (Invitrogen L3000008), and the pool of cells was selected with 5 μg/mL blasticidin (Sigma 203350) for ∼10 days prior to subcloning by limited dilution and isolation of clonal mESC lines.



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