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plenti cmv blast dest  (Addgene inc)


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    Structured Review

    Addgene inc plenti cmv blast dest
    Plenti Cmv Blast Dest, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/basic+local+alignment+sequence+tool+(blast)/ZE12+sonic+hedgehog+CMV+(Plasmid+%2317061)/pm33753928-415-19-40
    Average 93 stars, based on 18 article reviews
    plenti cmv blast dest - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Ribonuclease inhibitor and angiogenin system regulates cell type–specific global translation
    Article Snippet: .. To generate full-length GFP-ANG plasmid, the human ANG full coding sequence (ORIGEN RC 208874) was cloned into the entry vector pENTR4-GFP-C1 (W392-1, Addgene) and then recombined into the destination vector pLenti CMV Blast DEST (706-1, Addgene) plasmid using the Gateway cloning method. .. We purchased Renilla reporter constructs containing the promoter and 5′UTRs of eEF2 with either WT ( pIS1-Eef25UTR-renilla ; 38235) or mutant TOP sequences ( pIS1-Eef25UTR-TOPmut-renilla ; 38236) from Addgene.

    Article Title: Coupling cellular drug-target engagement to downstream pharmacology with CeTEAM
    Article Snippet: .. PARP1 L713F was subcloned from pET28-PARP1 L713F into pENTR1a-C-GFP by flanking SalI/NotI restriction sites and subsequently transferred to pINDUCER20, pCW57.1 (a gift from David Root; Addgene plasmid # 41393), or pLenti CMV Blast DEST (706-1), which was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 17451) . .. To generate pENTR1a-C-nLuc, nanoLuc (gBlock, IDT DNA) was subcloned into pENTR1a by flanking XbaI/XhoI restriction sites.

    Article Title: Coupling cellular drug-target engagement to downstream pharmacology with CeTEAM.
    Article Snippet: .. PARP1 L713F was subcloned from pET28-PARP1 L713F21 into pENTR1a-C-GFP by flanking SalI/NotI restriction sites and subsequently transferred to pINDUCER20, pCW57.1 (a gift fromDavid Root; Addgene plasmid # 41393), or pLenti CMV Blast DEST (706-1), which was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 17451)87. .. To generate pENTR1a-C-nLuc, nanoLuc (gBlock, IDT DNA) was subcloned into pENTR1a by flanking XbaI/XhoI restriction sites.

    Article Title: A Novel Metastatic Estrogen Receptor-Expressing Breast Cancer Model with Antiestrogen Responsiveness.
    Article Snippet: Restriction analysis using Noti-HF (NEB #R3189L, 20K U/mL, Lot 10030794), NruI-HF (NEB #R3192S, 20K U/mL, Lot 10030601), and AgeI-HF (NEB #R3552S, 20K U/mL, Lot 10028839) was performed with a CutSmart Buffer at 37 ◦C for 5–15 min or overnight to verify the correct orientation of the insert. .. The ERα-GFP expression cassette was then transferred from the entry clone to a Gateway compatible lentivirus transfer vector (pLenti CMV Blast DEST (706-1)), a gift from Eric Campeau and Paul Kaufman (Addgene plasmid #17451; http://n2t.net/addgene:17451 (accessed on 11 September 2019); RRID: Addgene_17451, [10]) using LR Clonase II. .. The transfer vector was then packaged into a lentivirus using the pPACKH1 HIV third generation lentiviral expression system kit and PureFection reagent (both from System Biosciences, Palo Alto, CA, USA) into HEK293-T cells (ATCC, Manassas, VA, USA).

    Article Title: Coupling cellular drug-target engagement to downstream pharmacology with CeTEAM
    Article Snippet: .. PARP1 L713F was subcloned from pET28-PARP1 L713F into pENTR1a-C-GFP by flanking SalI/NotI restriction sites and subsequently transferred to pINDUCER20, pCW57.1 (a gift from David Root; Addgene plasmid # 41393), or pLenti CMV Blast DEST (706-1), which was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 17451) . .. To generate pENTR1a-C-nLuc, nanoLuc (gBlock, IDT DNA) was subcloned into pENTR1a by flanking XbaI/XhoI restriction sites.

    Article Title: Ribonuclease Inhibitor and Angiogenin collaboratively regulate cell-type-specific global translation
    Article Snippet: .. To generate Full length GFP-ANG plasmid, the human Angiogenin (ANG) full coding sequence (ORIGEN RC 208874) was cloned into the entry vector pENTR4-GFP-C1 (W392-1, Addgene) and then recombined into the destination vector pLenti CMV Blast DEST (706-1, Addgene) plasmid using the Gateway cloning method. ..

    Sequencing:

    Article Title: Ribonuclease inhibitor and angiogenin system regulates cell type–specific global translation
    Article Snippet: .. To generate full-length GFP-ANG plasmid, the human ANG full coding sequence (ORIGEN RC 208874) was cloned into the entry vector pENTR4-GFP-C1 (W392-1, Addgene) and then recombined into the destination vector pLenti CMV Blast DEST (706-1, Addgene) plasmid using the Gateway cloning method. .. We purchased Renilla reporter constructs containing the promoter and 5′UTRs of eEF2 with either WT ( pIS1-Eef25UTR-renilla ; 38235) or mutant TOP sequences ( pIS1-Eef25UTR-TOPmut-renilla ; 38236) from Addgene.

    Article Title: Ribonuclease Inhibitor and Angiogenin collaboratively regulate cell-type-specific global translation
    Article Snippet: .. To generate Full length GFP-ANG plasmid, the human Angiogenin (ANG) full coding sequence (ORIGEN RC 208874) was cloned into the entry vector pENTR4-GFP-C1 (W392-1, Addgene) and then recombined into the destination vector pLenti CMV Blast DEST (706-1, Addgene) plasmid using the Gateway cloning method. ..

    Clone Assay:

    Article Title: Ribonuclease inhibitor and angiogenin system regulates cell type–specific global translation
    Article Snippet: .. To generate full-length GFP-ANG plasmid, the human ANG full coding sequence (ORIGEN RC 208874) was cloned into the entry vector pENTR4-GFP-C1 (W392-1, Addgene) and then recombined into the destination vector pLenti CMV Blast DEST (706-1, Addgene) plasmid using the Gateway cloning method. .. We purchased Renilla reporter constructs containing the promoter and 5′UTRs of eEF2 with either WT ( pIS1-Eef25UTR-renilla ; 38235) or mutant TOP sequences ( pIS1-Eef25UTR-TOPmut-renilla ; 38236) from Addgene.

    Article Title: Ribonuclease Inhibitor and Angiogenin collaboratively regulate cell-type-specific global translation
    Article Snippet: .. To generate Full length GFP-ANG plasmid, the human Angiogenin (ANG) full coding sequence (ORIGEN RC 208874) was cloned into the entry vector pENTR4-GFP-C1 (W392-1, Addgene) and then recombined into the destination vector pLenti CMV Blast DEST (706-1, Addgene) plasmid using the Gateway cloning method. ..

    Cloning:

    Article Title: Ribonuclease inhibitor and angiogenin system regulates cell type–specific global translation
    Article Snippet: .. To generate full-length GFP-ANG plasmid, the human ANG full coding sequence (ORIGEN RC 208874) was cloned into the entry vector pENTR4-GFP-C1 (W392-1, Addgene) and then recombined into the destination vector pLenti CMV Blast DEST (706-1, Addgene) plasmid using the Gateway cloning method. .. We purchased Renilla reporter constructs containing the promoter and 5′UTRs of eEF2 with either WT ( pIS1-Eef25UTR-renilla ; 38235) or mutant TOP sequences ( pIS1-Eef25UTR-TOPmut-renilla ; 38236) from Addgene.

    Article Title: Cytidine Deaminase Resolves Replicative Stress and Protects Pancreatic Cancer from DNA-Targeting Drugs
    Article Snippet: .. Lentiviral expression constructs were obtained by cloning 3xFLAG-CDA, CDAE67Q, and RLuc8 ORFs into pCMV blasticidin DEST vector (Addgene #706–1) using the Gateway strategy (Invitrogen). ..

    Article Title: Ribonuclease Inhibitor and Angiogenin collaboratively regulate cell-type-specific global translation
    Article Snippet: .. To generate Full length GFP-ANG plasmid, the human Angiogenin (ANG) full coding sequence (ORIGEN RC 208874) was cloned into the entry vector pENTR4-GFP-C1 (W392-1, Addgene) and then recombined into the destination vector pLenti CMV Blast DEST (706-1, Addgene) plasmid using the Gateway cloning method. ..

    Article Title: Cytidine deaminase resolves replicative stress and protects pancreatic cancer from DNA-targeting drugs
    Article Snippet: .. Lentiviral expression constructs were obtained by cloning 3xFLAG-CDA, CDAE67Q, and RLuc8 ORFs into pCMV blasticidin DEST vector (Addgene #706–1) using the Gateway strategy (Invitrogen). ..

    Expressing:

    Article Title: Cytidine Deaminase Resolves Replicative Stress and Protects Pancreatic Cancer from DNA-Targeting Drugs
    Article Snippet: .. Lentiviral expression constructs were obtained by cloning 3xFLAG-CDA, CDAE67Q, and RLuc8 ORFs into pCMV blasticidin DEST vector (Addgene #706–1) using the Gateway strategy (Invitrogen). ..

    Article Title: A Novel Metastatic Estrogen Receptor-Expressing Breast Cancer Model with Antiestrogen Responsiveness.
    Article Snippet: Restriction analysis using Noti-HF (NEB #R3189L, 20K U/mL, Lot 10030794), NruI-HF (NEB #R3192S, 20K U/mL, Lot 10030601), and AgeI-HF (NEB #R3552S, 20K U/mL, Lot 10028839) was performed with a CutSmart Buffer at 37 ◦C for 5–15 min or overnight to verify the correct orientation of the insert. .. The ERα-GFP expression cassette was then transferred from the entry clone to a Gateway compatible lentivirus transfer vector (pLenti CMV Blast DEST (706-1)), a gift from Eric Campeau and Paul Kaufman (Addgene plasmid #17451; http://n2t.net/addgene:17451 (accessed on 11 September 2019); RRID: Addgene_17451, [10]) using LR Clonase II. .. The transfer vector was then packaged into a lentivirus using the pPACKH1 HIV third generation lentiviral expression system kit and PureFection reagent (both from System Biosciences, Palo Alto, CA, USA) into HEK293-T cells (ATCC, Manassas, VA, USA).

    Article Title: Cytidine deaminase resolves replicative stress and protects pancreatic cancer from DNA-targeting drugs
    Article Snippet: .. Lentiviral expression constructs were obtained by cloning 3xFLAG-CDA, CDAE67Q, and RLuc8 ORFs into pCMV blasticidin DEST vector (Addgene #706–1) using the Gateway strategy (Invitrogen). ..

    Construct:

    Article Title: Cytidine Deaminase Resolves Replicative Stress and Protects Pancreatic Cancer from DNA-Targeting Drugs
    Article Snippet: .. Lentiviral expression constructs were obtained by cloning 3xFLAG-CDA, CDAE67Q, and RLuc8 ORFs into pCMV blasticidin DEST vector (Addgene #706–1) using the Gateway strategy (Invitrogen). ..

    Article Title: Cytidine deaminase resolves replicative stress and protects pancreatic cancer from DNA-targeting drugs
    Article Snippet: .. Lentiviral expression constructs were obtained by cloning 3xFLAG-CDA, CDAE67Q, and RLuc8 ORFs into pCMV blasticidin DEST vector (Addgene #706–1) using the Gateway strategy (Invitrogen). ..



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