Review




Structured Review

Amaxa basic fibroblast nucleofector kit

Basic Fibroblast Nucleofector Kit, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/basic+fibroblast+nucleofector+kit/basic+nucleofector+kit+for+primary+mammalian+fibroblasts/pmc10415803-24-1-0
Average 90 stars, based on 1 article reviews
basic fibroblast nucleofector kit - by Bioz Stars, 2026-10
90/100 stars

Images

1) Product Images from "Exosomal release of the virus-encoded chemokine receptor US28 contributes to chemokine scavenging"

Article Title: Exosomal release of the virus-encoded chemokine receptor US28 contributes to chemokine scavenging

Journal: iScience

doi: 10.1016/j.isci.2023.107412


Figure Legend Snippet:

Techniques Used: Virus, Recombinant, Luciferase, Software

Related Articles

Transfection:

Article Title: Isoform-specific characterization of class I histone deacetylases and their therapeutic modulation in pulmonary hypertension.
Article Snippet: .. Human vascular cells were transfected with isoform-specific siRNAs targeting HDACs or target genes using either Amaxa Basic Nucleofector Kit for Primary Mammalian Fibroblasts or Lipofectamine 3000 Transfection Reagent (Invitrogen), according to the manufacturer’s protocol. ..

Article Title: Isoform-specific characterization of class I histone deacetylases and their therapeutic modulation in pulmonary hypertension.
Article Snippet: .. Human vascular cells or ECV304 or HEK293 were transiently transfected with specific plasmids overexpressing HDAC1 (Sino Biological Inc.), HDAC2 (received as a gift from Prof. Edward Seto, George Washington Cancer Center), HDAC8 (OriGene Technologies Inc.) or LEF1 (Sino Biological Inc.) using either Amaxa Basic Nucleofecto Kit for Primary Mammalian Fibroblasts or TurboFect Transfection Reagent (Thermo Scientific), according to the manufacturer’s protocol. ..

Article Title: Isoform-specific characterization of class I histone deacetylases and their therapeutic modulation in pulmonary hypertension
Article Snippet: .. Human vascular cells were transfected with isoform-specific siRNAs targeting HDACs or target genes using either Amaxa Basic Nucleofector Kit for Primary Mammalian Fibroblasts or Lipofectamine 3000 Transfection Reagent (Invitrogen), according to the manufacturer’s protocol. ..

Article Title: The role of chemerin and ChemR23 in stimulating the invasion of squamous oesophageal cancer cells.
Article Snippet: .. Myofibroblasts were transfected using Amaxa Fibroblast Nucleofector kits (Amaxa, Köln, Germany) with scrambled controls (Sigma-Aldrich, Dorset, UK) or previously validated silencing RNAs (siRNA) for chemerin, or ChemR23, using nucleofection as previously described (Kumar et al, 2014). ..

Article Title: RhoB is a component of the human cytomegalovirus assembly complex and is required for efficient viral production.
Article Snippet: .. For live cell imaging, the HFFs were transfected with the mRFP-RhoB vector using the Amaxa Nucleofector kit for primary mammalian fibroblasts according to the manufacturer’s instructions (Amaxa Biosystems, Cologne, Germany). .. Cells were electroporated in suspension, plated in chambers with coverslip quality glass bottoms (LabTek; Nunc) and infected with the recombinant virus, UL32EGFP-HCMV-TB40, the following day.

Article Title: Regulation and function of SIRT1 in rheumatoid arthritis synovial fibroblasts.
Article Snippet: RASF were stimulated with 5 % CSE or 10 ng/ml TNFα (R&D systems) for 24 h. .. RASF were transfected using the Basic Nucleofector Kit for Primary Mammalian Fibroblasts (Amaxa) according to the manufacturer’s instructions. .. In silencing experiments, 100 μM of validated siRNA for SIRT1 or control siRNA (Invitrogen) was used.

Article Title: Increased Expression of Chemerin in Squamous Esophageal Cancer Myofibroblasts and Role in Recruitment of Mesenchymal Stromal Cells
Article Snippet: .. Cells were transfected using Amaxa Fibroblasts Nucleofector kits (Amaxa; Köln, Germany) and AmaxaTM Human MSC Nucleofector kits (Amaxa; Köln, Germany) according to manufacturer's instructions. ..

Live Cell Imaging:

Article Title: RhoB is a component of the human cytomegalovirus assembly complex and is required for efficient viral production.
Article Snippet: .. For live cell imaging, the HFFs were transfected with the mRFP-RhoB vector using the Amaxa Nucleofector kit for primary mammalian fibroblasts according to the manufacturer’s instructions (Amaxa Biosystems, Cologne, Germany). .. Cells were electroporated in suspension, plated in chambers with coverslip quality glass bottoms (LabTek; Nunc) and infected with the recombinant virus, UL32EGFP-HCMV-TB40, the following day.

Plasmid Preparation:

Article Title: RhoB is a component of the human cytomegalovirus assembly complex and is required for efficient viral production.
Article Snippet: .. For live cell imaging, the HFFs were transfected with the mRFP-RhoB vector using the Amaxa Nucleofector kit for primary mammalian fibroblasts according to the manufacturer’s instructions (Amaxa Biosystems, Cologne, Germany). .. Cells were electroporated in suspension, plated in chambers with coverslip quality glass bottoms (LabTek; Nunc) and infected with the recombinant virus, UL32EGFP-HCMV-TB40, the following day.



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Image Search Results


Journal: iScience

Article Title: Exosomal release of the virus-encoded chemokine receptor US28 contributes to chemokine scavenging

doi: 10.1016/j.isci.2023.107412

Figure Lengend Snippet:

Article Snippet: Amaxa basic fibroblast nucleofector kit , Lonza , Cat. #: VPI-1002.

Techniques: Virus, Recombinant, Luciferase, Software