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barcode oligonucleotides (New England Biolabs)

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New England Biolabs barcode oligonucleotides
Barcode Oligonucleotides, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4523 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/barcoding+primers/Nuclease-free+Water/pm41776501-44-26-36
Average 99 stars, based on 4523 article reviews

barcode oligonucleotides - by Bioz Stars, 2026-11

99/100 stars

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Article Title: Probe‐Free, Primer‐Programmed Amplicon Encoding Enables Nanopore‐Based Multiplex Detection With Deep Learning Decoding
Article Snippet: Key materials, 100 bp DNA Ladder (#N0467S), Gel Loading Dye, Purple (6X) (#B7025), and nuclease‐free water (B1500), were purchased from New England Biolabs Inc. (NEB).

Article Title: Single-molecule chromatin tracing reveals a diversity of megabase heterochromatin domains.
Article Snippet: For pre-hybridization, a 20 μl reaction (4 μl primary probe pool, 1 μl secondary FLAP-Y probe, 2 μl 10× NEBuffer 3, 13 μl nuclease-free water) was thermocycled (85 °C, 3 min; 65 °C, 3 min; 25 °C, 5 min) and stored at 4 °C (ref. 83).

Article Title: Scaffold RNA Reengineering for a Leakage‐Suppressed CRISPR/Cas12a Cascade System Toward Precise Nucleic Acid Diagnostics
Article Snippet: A 25 μL Cas12a reaction pre‐mixture was prepared, containing Cas12a (12.5 n m ), scaffold RNA (10 n m ), spacer RNA (10 n m ), substrate (10 n m ), TB‐Reporter (1000 n m ), and RNase‐free water in 1× NEBuffer TM r2.1.

Article Title: Portable and sensitive detection of peanut allergen Ara h 1 via a CRISPR/Cas12a-integrated personal glucose meter using polydopamine-functionalized pipette tips.
Article Snippet: A portable biosensor for the peanut allergen Ara h 1 was developed by integrating a polydopaminefunctionalized pipette tip, CRISPR/Cas12a signal amplification, and a personal glucose meter.. Ara h 1 binding induced partial displacement of a cDNA activator, which activated Cas12a trans-cleavage and released glucose oxidase from GOx–DNA conjugates.. The released enzyme catalyzed glucose oxidation, producing a quantitative decrease in glucose concentration.

Article Title: Modulation of the gut microbiome and resistome by antibiotic use, but not by subsequent 2'-fucosyllactose intake, in adults with overweight or obesity.
Article Snippet: Tagmentation was performed by adding 20 μl nuclease–free water, 1 μl pre-determined barcode (1–12A) and 25 μl 2× Q5 HotStart master mix (New England Biolabs).

Article Title: Programmable DNA protonuclei reveal environmental context on protein phase separation
Article Snippet: Thermostable inorganic pyrophosphatase (TIPP, 2000 U mL ─1 ) and nuclease-free water were purchased from New England Biolabs (NEB), dNTPs (100 mM–110 mM) were purchased from Jena Bioscience.

Recombinant:

Article Title: Paralogous mutations in the Plasmodium falciparum surface anion channel associate with reduced protection due to sickle hemoglobin.
Article Snippet: .. sWGA reactions were performed as previously described with a few minor modifications.39 In addition to the originally published primer set 10A, 6A was also used as trialled previously.39,40 To a final volume of 50μL an sWGA master mix was added to the 50ng of McrBC reaction digest (normalised using nuclease free water) with the concentrations of 1× NEB Phi29 buffer, 0.1mg/mL bovine serum albumin or recombinant albumin, 2.5 μM of each primer pool, 1mM of dNTP mixture and 10 units of Phi29 enzyme. ..

Plasmid Preparation:

Article Title: A dual-action bacteriophage protein Ped1 degrades DNA and blocks replication in Pseudomonas aeruginosa.
Article Snippet: In vitro protein synthesis was performed using the PURExpress In Vitro Protein Synthesis Kit (NEB) according to the manufacturer’s instructions. .. Each 25-μL reaction contained 10 μL Solution A, 7.5 μL Solution B, 250 ng plasmid DNA template (e.g., pET28aped1-flag), 20 units of Murine RNase Inhibitor (NEB), and nuclease-free water to volume. ..