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Pyrosequencing Inc barcoded 16s rrna pyrosequencing immunoassays
Barcoded 16s Rrna Pyrosequencing Immunoassays, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bar-coded+high-throughput+pyrosequencing/16s+pyrosequencing+rrna/pmc12653261-14-8-11
Average 86 stars, based on 1 article reviews
barcoded 16s rrna pyrosequencing immunoassays - by Bioz Stars, 2026-10
86/100 stars

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Article Title: Nitrification potential and ammonia-oxidizing archaeal diversity in three contrasting acidic soils of eastern China.
Article Snippet: 1 Acidic soils are widespread and are further acidified by intensive nitrogen fertilization.. 2 However, the ammonia-oxidizing microorganisms that drive nitrification under low pH 3 remain insufficiently understood.. Here, we investigated nitrification potential and the 4 responsible nitrifying microorganisms in three representative acidic soils of eastern 5 China (forest soil, red paddy soil, and tea garden soil), sampling both surface (0–10 cm) 6 and subsurface (10–20 cm) layers.

Article Title: Land-use types shape soil bacterial communities, co-occurrence networks, and predicted functions in karst ecosystems.
Article Snippet: 55.Will, C. et al. Horizon-Specific Bacterial Community Composition of German Grassland Soils, as Revealed by Pyrosequencing-Based Analysis of 16S rRNA

Article Title: Effect of Dietary Calcium Nitrate Addition on Methane Emission, Nitrogen Excretion, and Ruminal Fermentation Parameters and Microbiota in Liuyang Black Goats
Article Snippet: Additionally, another two aliquots had been preserved at −80 °C for 16S rRNA pyrosequencing.

Amplification:

Article Title: Effects of Long-term Weightless Stimulating by Tail Suspension on the Gut Microbiota as well as the Gut-liver Axis Homeostasis in Rat.
Article Snippet: Fecal DNA was extracted via Fast DNA SPIN extraction kits (MP Biomedicals, Santa Ana, CA, USA), and its quantity and quality were verified using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and agarose gel electrophoresis, respectively. .. The V3–V4 region of the bacterial 16S rRNA gene was amplified via PCR for 16S rDNA amplicon pyrosequencing on the Illumina MiSeq platform[14]. ..

Article Title: Microbial Communities Powering Plant-Microbial Fuel Cells: Diversity, Functions and Biotechnological Perspectives.
Article Snippet: .. Microbial communities analysis method References 16S rRNA gene- based DGGE band sequencing (Sanger) Kaku et al. (2008) 16S rRNA gene- based methods: DGGE, T- RFLP, Sanger clone libraries De Schamphelaire et al. (2010) 16S rRNA gene amplicon 454 pyrosequencing and qRTPCR; FISH- CLSM (rRNAtargeted probes) Timmers et al. (2012) 16S rRNA gene amplicon pyrosequencing, Illuminabased shotgun metagenomics (HiSeq) Kouzuma et al. (2013) qPCR targeting archaeal 16S rRNA genes (group- specific primers) Arends et al. (2014) 16S rRNA gene amplicon 454 pyrosequencing Ahn et al. (2014) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries (PMFC- A2) Cabezas et al. (2015) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries, 454 pyrosequencing (bacteria) Lin and Lu (2015) 16S rRNA gene amplicon 454 pyrosequencing (bacteria), 16S rRNA gene Sanger- based clone libraries (archaea) Lu et al. (2015) 16S rRNA gene amplicon 454 pyrosequencing Ueoka et al. (2016) 16S rRNA gene amplicon 454 pyrosequencing Tapia et al. (2017) Selective plating, biochemical identification Azri et al. (2018) Illumina 16S rRNA amplicon sequencing (HiSeq) Liu et al. (2018) Illumina 16S rRNA amplicon sequencing Sudirjo et al. (2019) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan and Yu (2021) Isolation and Sanger- based 16S rRNA sequencing Arulmani et al. (2021) Illumina 16S rRNA amplicon sequencing Tongphanpharn et al. (2023) (Continues) Microbial communities analysis method References Illumina 16S rRNA amplicon sequencing (MiSeq) Sarma et al. (2024) Illumina 16S rRNA amplicon sequencing (MiSeq) Chen et al. (2024) Illumina 16S rRNA amplicon sequencing (NovaSeq) Gan et al. (2025) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan et al. (2025) TABLE 2 | (Continued) 17517915, 2026, 2, D ow nloaded from https://envirom icro-journals.onlinelibrary.w iley.com /doi/10.1111/1751-7915.70310, W iley O nline L ibrary on [17/02/2026]. ..

Polymerase Chain Reaction:

Article Title: Effects of Long-term Weightless Stimulating by Tail Suspension on the Gut Microbiota as well as the Gut-liver Axis Homeostasis in Rat.
Article Snippet: Fecal DNA was extracted via Fast DNA SPIN extraction kits (MP Biomedicals, Santa Ana, CA, USA), and its quantity and quality were verified using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and agarose gel electrophoresis, respectively. .. The V3–V4 region of the bacterial 16S rRNA gene was amplified via PCR for 16S rDNA amplicon pyrosequencing on the Illumina MiSeq platform[14]. ..

Quantitative RT-PCR:

Article Title: Glycoside hydrolase-mediated utilization of Poria cocos polysaccharide enriches Lactobacillus gasseri and activates the AhR-IL-22 axis to attenuate DSS-induced colitis.
Article Snippet: .. 16S rRNA pyrosequencing technology of fecal samples from NC, DSS, L. g 10 7 CFU, L. g 10 9 CFU and L. g10 11 CFU groups of mice. (A) Venn diagram. (B) Chao1 index, Shannon index, Simpson index, observed ASVs from different mouse groups (n = 6, Data are presented as the median ± IQR,by Kruskal–Wallis (KW) with Dunn Post-hoc pairwise test). (C-D) Bray–Curtis-based PCoA and NMDS were performed to evaluate β-diversity (n = 6). (E) UPGMA clustering (Bray Curtis) and relative abundance of phylum level (top 15 taxa, n = 6). (F) Relative abundance of the identified fecal microbiota at the genus level (top 30 taxa, n = 6). (G) Lactobacillus. (H) Desulfovibrio. (I) Rikenella. (J) Parasutterella. (K) Faecalibaculum. (L) Allobaculum. (M) Bacteroides. (N) RT- qPCR of (Reg3γ,Reg3β) in colonic tissue (n = 6), groups treated with low/medium/high-dose L. gasseri (107, 109, and 1011CFU). ..

Bacteria:

Article Title: The Antibacterial Mechanism of Baicalin and Its Solubilization Strategy
Article Snippet: .. Pyrosequencing of 16S rRNA genes in rat feces revealed that baicalin effectively increased the abundance of beneficial intestinal symbiotic bacteria ( Ligilactobacillus , Lactobacillus and Bacteroides ), but decreased the abundance of harmful intestinal symbiotic bacteria ( Muribaculaceae and Alistipes ) [ ]. ..

Article Title: The Antibacterial Mechanism of Baicalin and Its Solubilization Strategy.
Article Snippet: .. Pyrosequencing of 16S rRNA genes in rat feces revealed that baicalin effectively increased the abundance of beneficial intestinal symbiotic bacteria (Ligilactobacillus, Lactobacillus and Bacteroides), but decreased the abundance of harmful intestinal symbiotic bacteria (Muribaculaceae and Alistipes) [81]. ..

Article Title: Microbial Communities Powering Plant-Microbial Fuel Cells: Diversity, Functions and Biotechnological Perspectives.
Article Snippet: .. Microbial communities analysis method References 16S rRNA gene- based DGGE band sequencing (Sanger) Kaku et al. (2008) 16S rRNA gene- based methods: DGGE, T- RFLP, Sanger clone libraries De Schamphelaire et al. (2010) 16S rRNA gene amplicon 454 pyrosequencing and qRTPCR; FISH- CLSM (rRNAtargeted probes) Timmers et al. (2012) 16S rRNA gene amplicon pyrosequencing, Illuminabased shotgun metagenomics (HiSeq) Kouzuma et al. (2013) qPCR targeting archaeal 16S rRNA genes (group- specific primers) Arends et al. (2014) 16S rRNA gene amplicon 454 pyrosequencing Ahn et al. (2014) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries (PMFC- A2) Cabezas et al. (2015) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries, 454 pyrosequencing (bacteria) Lin and Lu (2015) 16S rRNA gene amplicon 454 pyrosequencing (bacteria), 16S rRNA gene Sanger- based clone libraries (archaea) Lu et al. (2015) 16S rRNA gene amplicon 454 pyrosequencing Ueoka et al. (2016) 16S rRNA gene amplicon 454 pyrosequencing Tapia et al. (2017) Selective plating, biochemical identification Azri et al. (2018) Illumina 16S rRNA amplicon sequencing (HiSeq) Liu et al. (2018) Illumina 16S rRNA amplicon sequencing Sudirjo et al. (2019) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan and Yu (2021) Isolation and Sanger- based 16S rRNA sequencing Arulmani et al. (2021) Illumina 16S rRNA amplicon sequencing Tongphanpharn et al. (2023) (Continues) Microbial communities analysis method References Illumina 16S rRNA amplicon sequencing (MiSeq) Sarma et al. (2024) Illumina 16S rRNA amplicon sequencing (MiSeq) Chen et al. (2024) Illumina 16S rRNA amplicon sequencing (NovaSeq) Gan et al. (2025) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan et al. (2025) TABLE 2 | (Continued) 17517915, 2026, 2, D ow nloaded from https://envirom icro-journals.onlinelibrary.w iley.com /doi/10.1111/1751-7915.70310, W iley O nline L ibrary on [17/02/2026]. ..

Denaturing Gradient Gel Electrophoresis:

Article Title: Microbial Communities Powering Plant-Microbial Fuel Cells: Diversity, Functions and Biotechnological Perspectives.
Article Snippet: .. Microbial communities analysis method References 16S rRNA gene- based DGGE band sequencing (Sanger) Kaku et al. (2008) 16S rRNA gene- based methods: DGGE, T- RFLP, Sanger clone libraries De Schamphelaire et al. (2010) 16S rRNA gene amplicon 454 pyrosequencing and qRTPCR; FISH- CLSM (rRNAtargeted probes) Timmers et al. (2012) 16S rRNA gene amplicon pyrosequencing, Illuminabased shotgun metagenomics (HiSeq) Kouzuma et al. (2013) qPCR targeting archaeal 16S rRNA genes (group- specific primers) Arends et al. (2014) 16S rRNA gene amplicon 454 pyrosequencing Ahn et al. (2014) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries (PMFC- A2) Cabezas et al. (2015) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries, 454 pyrosequencing (bacteria) Lin and Lu (2015) 16S rRNA gene amplicon 454 pyrosequencing (bacteria), 16S rRNA gene Sanger- based clone libraries (archaea) Lu et al. (2015) 16S rRNA gene amplicon 454 pyrosequencing Ueoka et al. (2016) 16S rRNA gene amplicon 454 pyrosequencing Tapia et al. (2017) Selective plating, biochemical identification Azri et al. (2018) Illumina 16S rRNA amplicon sequencing (HiSeq) Liu et al. (2018) Illumina 16S rRNA amplicon sequencing Sudirjo et al. (2019) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan and Yu (2021) Isolation and Sanger- based 16S rRNA sequencing Arulmani et al. (2021) Illumina 16S rRNA amplicon sequencing Tongphanpharn et al. (2023) (Continues) Microbial communities analysis method References Illumina 16S rRNA amplicon sequencing (MiSeq) Sarma et al. (2024) Illumina 16S rRNA amplicon sequencing (MiSeq) Chen et al. (2024) Illumina 16S rRNA amplicon sequencing (NovaSeq) Gan et al. (2025) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan et al. (2025) TABLE 2 | (Continued) 17517915, 2026, 2, D ow nloaded from https://envirom icro-journals.onlinelibrary.w iley.com /doi/10.1111/1751-7915.70310, W iley O nline L ibrary on [17/02/2026]. ..

Sequencing:

Article Title: Microbial Communities Powering Plant-Microbial Fuel Cells: Diversity, Functions and Biotechnological Perspectives.
Article Snippet: .. Microbial communities analysis method References 16S rRNA gene- based DGGE band sequencing (Sanger) Kaku et al. (2008) 16S rRNA gene- based methods: DGGE, T- RFLP, Sanger clone libraries De Schamphelaire et al. (2010) 16S rRNA gene amplicon 454 pyrosequencing and qRTPCR; FISH- CLSM (rRNAtargeted probes) Timmers et al. (2012) 16S rRNA gene amplicon pyrosequencing, Illuminabased shotgun metagenomics (HiSeq) Kouzuma et al. (2013) qPCR targeting archaeal 16S rRNA genes (group- specific primers) Arends et al. (2014) 16S rRNA gene amplicon 454 pyrosequencing Ahn et al. (2014) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries (PMFC- A2) Cabezas et al. (2015) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries, 454 pyrosequencing (bacteria) Lin and Lu (2015) 16S rRNA gene amplicon 454 pyrosequencing (bacteria), 16S rRNA gene Sanger- based clone libraries (archaea) Lu et al. (2015) 16S rRNA gene amplicon 454 pyrosequencing Ueoka et al. (2016) 16S rRNA gene amplicon 454 pyrosequencing Tapia et al. (2017) Selective plating, biochemical identification Azri et al. (2018) Illumina 16S rRNA amplicon sequencing (HiSeq) Liu et al. (2018) Illumina 16S rRNA amplicon sequencing Sudirjo et al. (2019) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan and Yu (2021) Isolation and Sanger- based 16S rRNA sequencing Arulmani et al. (2021) Illumina 16S rRNA amplicon sequencing Tongphanpharn et al. (2023) (Continues) Microbial communities analysis method References Illumina 16S rRNA amplicon sequencing (MiSeq) Sarma et al. (2024) Illumina 16S rRNA amplicon sequencing (MiSeq) Chen et al. (2024) Illumina 16S rRNA amplicon sequencing (NovaSeq) Gan et al. (2025) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan et al. (2025) TABLE 2 | (Continued) 17517915, 2026, 2, D ow nloaded from https://envirom icro-journals.onlinelibrary.w iley.com /doi/10.1111/1751-7915.70310, W iley O nline L ibrary on [17/02/2026]. ..

Terminal Restriction Fragment Length Polymorphism:

Article Title: Microbial Communities Powering Plant-Microbial Fuel Cells: Diversity, Functions and Biotechnological Perspectives.
Article Snippet: .. Microbial communities analysis method References 16S rRNA gene- based DGGE band sequencing (Sanger) Kaku et al. (2008) 16S rRNA gene- based methods: DGGE, T- RFLP, Sanger clone libraries De Schamphelaire et al. (2010) 16S rRNA gene amplicon 454 pyrosequencing and qRTPCR; FISH- CLSM (rRNAtargeted probes) Timmers et al. (2012) 16S rRNA gene amplicon pyrosequencing, Illuminabased shotgun metagenomics (HiSeq) Kouzuma et al. (2013) qPCR targeting archaeal 16S rRNA genes (group- specific primers) Arends et al. (2014) 16S rRNA gene amplicon 454 pyrosequencing Ahn et al. (2014) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries (PMFC- A2) Cabezas et al. (2015) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries, 454 pyrosequencing (bacteria) Lin and Lu (2015) 16S rRNA gene amplicon 454 pyrosequencing (bacteria), 16S rRNA gene Sanger- based clone libraries (archaea) Lu et al. (2015) 16S rRNA gene amplicon 454 pyrosequencing Ueoka et al. (2016) 16S rRNA gene amplicon 454 pyrosequencing Tapia et al. (2017) Selective plating, biochemical identification Azri et al. (2018) Illumina 16S rRNA amplicon sequencing (HiSeq) Liu et al. (2018) Illumina 16S rRNA amplicon sequencing Sudirjo et al. (2019) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan and Yu (2021) Isolation and Sanger- based 16S rRNA sequencing Arulmani et al. (2021) Illumina 16S rRNA amplicon sequencing Tongphanpharn et al. (2023) (Continues) Microbial communities analysis method References Illumina 16S rRNA amplicon sequencing (MiSeq) Sarma et al. (2024) Illumina 16S rRNA amplicon sequencing (MiSeq) Chen et al. (2024) Illumina 16S rRNA amplicon sequencing (NovaSeq) Gan et al. (2025) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan et al. (2025) TABLE 2 | (Continued) 17517915, 2026, 2, D ow nloaded from https://envirom icro-journals.onlinelibrary.w iley.com /doi/10.1111/1751-7915.70310, W iley O nline L ibrary on [17/02/2026]. ..

Fluorescence In Situ Hybridization:

Article Title: Microbial Communities Powering Plant-Microbial Fuel Cells: Diversity, Functions and Biotechnological Perspectives.
Article Snippet: .. Microbial communities analysis method References 16S rRNA gene- based DGGE band sequencing (Sanger) Kaku et al. (2008) 16S rRNA gene- based methods: DGGE, T- RFLP, Sanger clone libraries De Schamphelaire et al. (2010) 16S rRNA gene amplicon 454 pyrosequencing and qRTPCR; FISH- CLSM (rRNAtargeted probes) Timmers et al. (2012) 16S rRNA gene amplicon pyrosequencing, Illuminabased shotgun metagenomics (HiSeq) Kouzuma et al. (2013) qPCR targeting archaeal 16S rRNA genes (group- specific primers) Arends et al. (2014) 16S rRNA gene amplicon 454 pyrosequencing Ahn et al. (2014) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries (PMFC- A2) Cabezas et al. (2015) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries, 454 pyrosequencing (bacteria) Lin and Lu (2015) 16S rRNA gene amplicon 454 pyrosequencing (bacteria), 16S rRNA gene Sanger- based clone libraries (archaea) Lu et al. (2015) 16S rRNA gene amplicon 454 pyrosequencing Ueoka et al. (2016) 16S rRNA gene amplicon 454 pyrosequencing Tapia et al. (2017) Selective plating, biochemical identification Azri et al. (2018) Illumina 16S rRNA amplicon sequencing (HiSeq) Liu et al. (2018) Illumina 16S rRNA amplicon sequencing Sudirjo et al. (2019) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan and Yu (2021) Isolation and Sanger- based 16S rRNA sequencing Arulmani et al. (2021) Illumina 16S rRNA amplicon sequencing Tongphanpharn et al. (2023) (Continues) Microbial communities analysis method References Illumina 16S rRNA amplicon sequencing (MiSeq) Sarma et al. (2024) Illumina 16S rRNA amplicon sequencing (MiSeq) Chen et al. (2024) Illumina 16S rRNA amplicon sequencing (NovaSeq) Gan et al. (2025) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan et al. (2025) TABLE 2 | (Continued) 17517915, 2026, 2, D ow nloaded from https://envirom icro-journals.onlinelibrary.w iley.com /doi/10.1111/1751-7915.70310, W iley O nline L ibrary on [17/02/2026]. ..

Confocal Laser Scanning Microscopy:

Article Title: Microbial Communities Powering Plant-Microbial Fuel Cells: Diversity, Functions and Biotechnological Perspectives.
Article Snippet: .. Microbial communities analysis method References 16S rRNA gene- based DGGE band sequencing (Sanger) Kaku et al. (2008) 16S rRNA gene- based methods: DGGE, T- RFLP, Sanger clone libraries De Schamphelaire et al. (2010) 16S rRNA gene amplicon 454 pyrosequencing and qRTPCR; FISH- CLSM (rRNAtargeted probes) Timmers et al. (2012) 16S rRNA gene amplicon pyrosequencing, Illuminabased shotgun metagenomics (HiSeq) Kouzuma et al. (2013) qPCR targeting archaeal 16S rRNA genes (group- specific primers) Arends et al. (2014) 16S rRNA gene amplicon 454 pyrosequencing Ahn et al. (2014) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries (PMFC- A2) Cabezas et al. (2015) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries, 454 pyrosequencing (bacteria) Lin and Lu (2015) 16S rRNA gene amplicon 454 pyrosequencing (bacteria), 16S rRNA gene Sanger- based clone libraries (archaea) Lu et al. (2015) 16S rRNA gene amplicon 454 pyrosequencing Ueoka et al. (2016) 16S rRNA gene amplicon 454 pyrosequencing Tapia et al. (2017) Selective plating, biochemical identification Azri et al. (2018) Illumina 16S rRNA amplicon sequencing (HiSeq) Liu et al. (2018) Illumina 16S rRNA amplicon sequencing Sudirjo et al. (2019) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan and Yu (2021) Isolation and Sanger- based 16S rRNA sequencing Arulmani et al. (2021) Illumina 16S rRNA amplicon sequencing Tongphanpharn et al. (2023) (Continues) Microbial communities analysis method References Illumina 16S rRNA amplicon sequencing (MiSeq) Sarma et al. (2024) Illumina 16S rRNA amplicon sequencing (MiSeq) Chen et al. (2024) Illumina 16S rRNA amplicon sequencing (NovaSeq) Gan et al. (2025) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan et al. (2025) TABLE 2 | (Continued) 17517915, 2026, 2, D ow nloaded from https://envirom icro-journals.onlinelibrary.w iley.com /doi/10.1111/1751-7915.70310, W iley O nline L ibrary on [17/02/2026]. ..

Metagenomics:

Article Title: Microbial Communities Powering Plant-Microbial Fuel Cells: Diversity, Functions and Biotechnological Perspectives.
Article Snippet: .. Microbial communities analysis method References 16S rRNA gene- based DGGE band sequencing (Sanger) Kaku et al. (2008) 16S rRNA gene- based methods: DGGE, T- RFLP, Sanger clone libraries De Schamphelaire et al. (2010) 16S rRNA gene amplicon 454 pyrosequencing and qRTPCR; FISH- CLSM (rRNAtargeted probes) Timmers et al. (2012) 16S rRNA gene amplicon pyrosequencing, Illuminabased shotgun metagenomics (HiSeq) Kouzuma et al. (2013) qPCR targeting archaeal 16S rRNA genes (group- specific primers) Arends et al. (2014) 16S rRNA gene amplicon 454 pyrosequencing Ahn et al. (2014) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries (PMFC- A2) Cabezas et al. (2015) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries, 454 pyrosequencing (bacteria) Lin and Lu (2015) 16S rRNA gene amplicon 454 pyrosequencing (bacteria), 16S rRNA gene Sanger- based clone libraries (archaea) Lu et al. (2015) 16S rRNA gene amplicon 454 pyrosequencing Ueoka et al. (2016) 16S rRNA gene amplicon 454 pyrosequencing Tapia et al. (2017) Selective plating, biochemical identification Azri et al. (2018) Illumina 16S rRNA amplicon sequencing (HiSeq) Liu et al. (2018) Illumina 16S rRNA amplicon sequencing Sudirjo et al. (2019) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan and Yu (2021) Isolation and Sanger- based 16S rRNA sequencing Arulmani et al. (2021) Illumina 16S rRNA amplicon sequencing Tongphanpharn et al. (2023) (Continues) Microbial communities analysis method References Illumina 16S rRNA amplicon sequencing (MiSeq) Sarma et al. (2024) Illumina 16S rRNA amplicon sequencing (MiSeq) Chen et al. (2024) Illumina 16S rRNA amplicon sequencing (NovaSeq) Gan et al. (2025) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan et al. (2025) TABLE 2 | (Continued) 17517915, 2026, 2, D ow nloaded from https://envirom icro-journals.onlinelibrary.w iley.com /doi/10.1111/1751-7915.70310, W iley O nline L ibrary on [17/02/2026]. ..

Real-time Polymerase Chain Reaction:

Article Title: Microbial Communities Powering Plant-Microbial Fuel Cells: Diversity, Functions and Biotechnological Perspectives.
Article Snippet: .. Microbial communities analysis method References 16S rRNA gene- based DGGE band sequencing (Sanger) Kaku et al. (2008) 16S rRNA gene- based methods: DGGE, T- RFLP, Sanger clone libraries De Schamphelaire et al. (2010) 16S rRNA gene amplicon 454 pyrosequencing and qRTPCR; FISH- CLSM (rRNAtargeted probes) Timmers et al. (2012) 16S rRNA gene amplicon pyrosequencing, Illuminabased shotgun metagenomics (HiSeq) Kouzuma et al. (2013) qPCR targeting archaeal 16S rRNA genes (group- specific primers) Arends et al. (2014) 16S rRNA gene amplicon 454 pyrosequencing Ahn et al. (2014) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries (PMFC- A2) Cabezas et al. (2015) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries, 454 pyrosequencing (bacteria) Lin and Lu (2015) 16S rRNA gene amplicon 454 pyrosequencing (bacteria), 16S rRNA gene Sanger- based clone libraries (archaea) Lu et al. (2015) 16S rRNA gene amplicon 454 pyrosequencing Ueoka et al. (2016) 16S rRNA gene amplicon 454 pyrosequencing Tapia et al. (2017) Selective plating, biochemical identification Azri et al. (2018) Illumina 16S rRNA amplicon sequencing (HiSeq) Liu et al. (2018) Illumina 16S rRNA amplicon sequencing Sudirjo et al. (2019) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan and Yu (2021) Isolation and Sanger- based 16S rRNA sequencing Arulmani et al. (2021) Illumina 16S rRNA amplicon sequencing Tongphanpharn et al. (2023) (Continues) Microbial communities analysis method References Illumina 16S rRNA amplicon sequencing (MiSeq) Sarma et al. (2024) Illumina 16S rRNA amplicon sequencing (MiSeq) Chen et al. (2024) Illumina 16S rRNA amplicon sequencing (NovaSeq) Gan et al. (2025) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan et al. (2025) TABLE 2 | (Continued) 17517915, 2026, 2, D ow nloaded from https://envirom icro-journals.onlinelibrary.w iley.com /doi/10.1111/1751-7915.70310, W iley O nline L ibrary on [17/02/2026]. ..

Isolation:

Article Title: Microbial Communities Powering Plant-Microbial Fuel Cells: Diversity, Functions and Biotechnological Perspectives.
Article Snippet: .. Microbial communities analysis method References 16S rRNA gene- based DGGE band sequencing (Sanger) Kaku et al. (2008) 16S rRNA gene- based methods: DGGE, T- RFLP, Sanger clone libraries De Schamphelaire et al. (2010) 16S rRNA gene amplicon 454 pyrosequencing and qRTPCR; FISH- CLSM (rRNAtargeted probes) Timmers et al. (2012) 16S rRNA gene amplicon pyrosequencing, Illuminabased shotgun metagenomics (HiSeq) Kouzuma et al. (2013) qPCR targeting archaeal 16S rRNA genes (group- specific primers) Arends et al. (2014) 16S rRNA gene amplicon 454 pyrosequencing Ahn et al. (2014) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries (PMFC- A2) Cabezas et al. (2015) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries, 454 pyrosequencing (bacteria) Lin and Lu (2015) 16S rRNA gene amplicon 454 pyrosequencing (bacteria), 16S rRNA gene Sanger- based clone libraries (archaea) Lu et al. (2015) 16S rRNA gene amplicon 454 pyrosequencing Ueoka et al. (2016) 16S rRNA gene amplicon 454 pyrosequencing Tapia et al. (2017) Selective plating, biochemical identification Azri et al. (2018) Illumina 16S rRNA amplicon sequencing (HiSeq) Liu et al. (2018) Illumina 16S rRNA amplicon sequencing Sudirjo et al. (2019) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan and Yu (2021) Isolation and Sanger- based 16S rRNA sequencing Arulmani et al. (2021) Illumina 16S rRNA amplicon sequencing Tongphanpharn et al. (2023) (Continues) Microbial communities analysis method References Illumina 16S rRNA amplicon sequencing (MiSeq) Sarma et al. (2024) Illumina 16S rRNA amplicon sequencing (MiSeq) Chen et al. (2024) Illumina 16S rRNA amplicon sequencing (NovaSeq) Gan et al. (2025) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan et al. (2025) TABLE 2 | (Continued) 17517915, 2026, 2, D ow nloaded from https://envirom icro-journals.onlinelibrary.w iley.com /doi/10.1111/1751-7915.70310, W iley O nline L ibrary on [17/02/2026]. ..



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