high throughput dna sequencing (Illumina Inc)

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Illumina Inc high throughput dna sequencing

Ploidy of isolates and spore clones. (A) Representative flow cytometry plots of YJS5845 (left), YJS5885 (right), and derived spore clones. All spore clones of YJS5845 and YJS5885 tested were diploid and haploid, respectively. The black arrows show the position of 1n, 2n, and 4n <t>DNA</t> content. Inset shows percentage of single cells, small-budded cells, and large-budded cells assessed by light microscopy. (B) Whole-genome <t>sequencing</t> was performed for YJS5845 and spore clones (Materials and Methods). YJS5845, and spore clones 5845-18a and 5845-28b, displayed aneuploidy for chromosomes (Chr) XIV, XI, and I, respectively.

High Throughput Dna Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 2700 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bar-coded+high-throughput+pyrosequencing/Nextera+DNA+Library+Prep/pmc06283166-185-8-15
Average 97 stars, based on 2700 article reviews

high throughput dna sequencing - by Bioz Stars, 2026-10

97/100 stars

Images

1) Product Images from "Incompatibilities in Mismatch Repair Genes MLH1-PMS1 Contribute to a Wide Range of Mutation Rates in Human Isolates of Baker’s Yeast"

Article Title: Incompatibilities in Mismatch Repair Genes MLH1-PMS1 Contribute to a Wide Range of Mutation Rates in Human Isolates of Baker’s Yeast

Journal: Genetics

doi: 10.1534/genetics.118.301550

Figure Legend Snippet: Ploidy of isolates and spore clones. (A) Representative flow cytometry plots of YJS5845 (left), YJS5885 (right), and derived spore clones. All spore clones of YJS5845 and YJS5885 tested were diploid and haploid, respectively. The black arrows show the position of 1n, 2n, and 4n DNA content. Inset shows percentage of single cells, small-budded cells, and large-budded cells assessed by light microscopy. (B) Whole-genome sequencing was performed for YJS5845 and spore clones (Materials and Methods). YJS5845, and spore clones 5845-18a and 5845-28b, displayed aneuploidy for chromosomes (Chr) XIV, XI, and I, respectively.

Techniques Used: Clone Assay, Flow Cytometry, Derivative Assay, Light Microscopy, Sequencing

Shotgun Sequencing:

Article Title: Gut Microbiota Affects Age-Related Plasma Metabolites.
Article Snippet: Extraction of DNA for sequencing was conducted at Diversigen (Corebiome; Minneapolis, MN, USA) using Qiagen PowerFecal Pro extraction kit. .. A DNA library was prepared using the Nextera library preparation kit (Illumina, San Diego, CA, USA), and shallow shotgun sequencing was conducted using the the Diversigen® BoosterShot® (New Brighton, MN, USA) pipeline with the Illumina NextSeq 500 instrument (Illumina, San Diego, USA). ..

Purification:

Article Title: Estimating the distribution of fitness effects of loss of heterozygosity (LOH) events using an engineered library of Saccharomyces cerevisiae
Article Snippet: Whole genome resequencing on an Illumina platform was performed on individual LOH library strains and control strains to determine the regions of LOH and the barcodes. .. Genomic DNA was extracted from overnight YPD liquid culture using the Puregene Yeast/Bacterium Kit (Qiagen) and purified by AMPure XP Beads (Beckman Coulter) at a 0.8x ratio before performing Illumina library preparation using the Nextera XT DNA Library Preparation Kit (Illumina) combined with Illumina DNA/RNA UD Indexes (Illumina). ..

DNA Library Preparation:

Article Title: A WGS workflow for identifying genetically modified and foodborne-pathogenic Bacillus isolates
Article Snippet: For the wt B. velezensis samples and the B. cereus s.l. datasets (excluding the B. cereus s.s. samples obtained from another study), DNA from bacterial isolates was extracted using Quick-DNATM HMW MagBead Kit (Zymo Research, Irvine, CA, USA) according to the manufacturer's instructions and then sequenced on the Illumina platform, following previously described protocol ( ). .. Short-read DNA libraries were prepared using the Nextera XT DNA library preparation kit (Illumina, San Diego, CA, USA) according to manufacturer's instructions. .. Sequencing was carried out on an Illumina MiSeq system with the V3 chemistry, obtaining 250 bp paired-end reads, and aiming for a theoretical coverage of 60× per sample, based on an average Bacillus genome size of 4.2 Mbp.

Sequencing:

Article Title: Population structure and spatial distribution of Mycobacterium tuberculosis complex in Catalonia
Article Snippet: .. DNA was extracted using the EZ-1 DSP Viral Kit (Qiagen), libraries were prepared with Nextera XT or Illumina DNA Prep kits, and sequencing was performed on Illumina MiSeq or NextSeq 1,000 platforms. ..

Article Title: Diverse genomic landscape of swine influenza A virus in England (2014-2021).
Article Snippet: Extracted total RNA was reverse- transcribed to generate double- stranded cDNA, which was then amplified using sequenceindependent single- primer amplification [32] and purified using AMPure beads (Beckman Coulter, Brea, CA, USA). .. Sequencing library preparation was performed using the Nextera DNA Library Prep Kit (Illumina, Cambridge, MA, USA) and sequenced using the NextSeq System (Illumina). ..

Sample Prep:

Article Title: Accelerated mutator phenotype in a clinical Aspergillus fumigatus isolate contributes to adaptive evolution
Article Snippet: .. For the first analysis of putative mutator isolate V130-14, a DNA library was prepared using the Nextera XT® DNA sample preparation kit (Illumina, USA). .. Subsequent sequencing was conducted in a paired end 2 × 150 bp mode using an Illumina NextSeq 500® machine (Illumina, USA) at the Department of Genetics in Radboudumc (Nijmegen, Netherlands).

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