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Promega lambda-gem-11 arms
Lambda Gem 11 Arms, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bamhi-digested+lambda-gem-11+arms/%CE%BBgem11/us07056888-498-7-9
Average 90 stars, based on 1 article reviews
lambda-gem-11 arms - by Bioz Stars, 2026-11
90/100 stars

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Article Title: Identification and manipulation of the pleuromutilin gene cluster from Clitopilus passeckerianus for increased rapid antibiotic production
Article Snippet: The DNA was then size-fractionated and cloned in Bam HI-linearized vector Lambda GEM-11 (Promega) following the vector’s instruction manual.

Article Title: Antibody to receptor on the surface of activated T-cells: ACT-4
Article Snippet: For example, a full-length polynucleotide corresponding to the sequence shown in FIG. 5 can be labeled and used as a hybridization probe to isolate genomic clones from a human genomic clone library in e.g., λEMBL4 or λGEM11 (Promega Corporation, Madison, Wis.); typical hybridization conditions for screening plaque lifts (Benton & Davis, Science 196:180 (1978)) can be: 50% formamide, 5×SSC or SSPE, 1-5× Denhardt's solution, 0.1-1% SDS, 100-200 μg sheared heterologous DNA or tRNA, 0-10% dextran sulfate, 1×105 to 1×107 cpm/ml of denatured probe with a specific activity of about 1×108 cpm/μg, and incubation at 42° C. for about 6-36 hours.

Article Title: The Tribolium decapentaplegic gene is similar in sequence, structure, and expression to the Drosophila dpp gene.
Article Snippet: forming Growth Factor-β (TGF-β) superfamily in the red flour beetle, Tribolium castaneum, in order to examine the evolutionary conservation of the structure and function of TGF-β-like genes during insect development.. A decapentaplegic-like gene of the TGF-β superfamily was isolated in Tribolium (Tc dpp) that is similar in sequence, organization, and expression to the Drosophila melanogaster dpp gene (Dm dpp).. Conserved features include a high degree of sequence similarity in both the pro-domain and mature domains of the encoded polypeptide.

Article Title: ORGANIZATION OF THE nif GENES OF THE NONHETEROCYSTOUS CYANOBACTERIUM TRICHODESMIUM SP. IMS101.
Article Snippet: An approximately 16-kb fragment of the Trichodesmium sp. IMS101 (a nonheterocystous filamentous cyanobacterium) “conventional” nif gene cluster was cloned and sequenced.. The gene organization of the Trichodesmium and Anabaena variabilis vegetative ( nif2 ) nitrogenase gene clusters spanning the region from nifB to nifW are similar except for the absence of two open reading frames (ORF3 and ORF1) in Trichodesmium .. The Trichodesmium nifEN genes encode a fused NifEN polypeptide that does not appear to be processed into individual NifE and NifN polypeptides.

Article Title: Polypeptides and polynucleotides from coagulase-negative staphylococci
Article Snippet: The sdrG gene was cloned from a λGem®-11 library of S. epidermidis strain K-28 generated with DNA that had been partially digested with Sau3A and ligated into the half-site XhoI arms of λGem®-11 (Promega).

Labeling:

Article Title: Human smooth muscle myosin heavy chain
Article Snippet: .. For illustration and not for limitation, SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, or SEQ ID NO:13 may be labeled and used as a hybridization probe to isolate genomic clones from a human or murine genomic clone library in EMBL4 or GEM11 (Promega Corporation, Madison, Wis.). ..

Hybridization:

Article Title: Human smooth muscle myosin heavy chain
Article Snippet: .. For illustration and not for limitation, SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, or SEQ ID NO:13 may be labeled and used as a hybridization probe to isolate genomic clones from a human or murine genomic clone library in EMBL4 or GEM11 (Promega Corporation, Madison, Wis.). ..

Clone Assay:

Article Title: Human smooth muscle myosin heavy chain
Article Snippet: .. For illustration and not for limitation, SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, or SEQ ID NO:13 may be labeled and used as a hybridization probe to isolate genomic clones from a human or murine genomic clone library in EMBL4 or GEM11 (Promega Corporation, Madison, Wis.). ..

Article Title: Antibodies to the polypeptide hormone phosphatonin
Article Snippet: .. Such a library consists e.g. of genomic DNA prepared from human blood cells, fractionized in fragments ranging from 5 kb to 50 kb, cloned into the lambda GEM 11 (Promega) phages. ..



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Promega bamhi-digested lambda-gem-11 arms
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