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Promega mboi
Mboi, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bamhi-digested+lambda+dash+ii+vector+arms/mboi/pm09723925-152-6-7
Average 90 stars, based on 1 article reviews
mboi - by Bioz Stars, 2026-09
90/100 stars

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Terminal Restriction Fragment Length Polymorphism:

Article Title: Microform-related community patterns of methane-cycling microbes in boreal Sphagnum bogs are site specific.
Article Snippet: Each 50-μl PCR reaction contained 0.5 μM of each primer, 200 μM of dNTPs and 1 U of DNA polymerase (Biotools B & M Labs S.A., Madrid, Spain) in 1 × reaction buffer and 1 μl of template DNA. .. Thermal cycling was started by initial denaturation at 94◦C for 3 min, followed by 40 cycles of denaturation at 94◦C for 30 s, annealing at 55◦C for 45 s, extension at 72◦C for 30 s and a final extension at 72◦C for 5 min. Products were digested for T-RFLP analysis with MboI (3 U, Promega) and HhaI (3 U, Promega) in a volume of 20 μl for 6 h. The enzymes were selected for optimal separation of methanogens common in northern peatlands based on previous sequence data from 11 peatlands (Martı́ et al. 2015). .. Digested products were ethanol precipitated, dissolved in 10 μl of Hi-Di formamide (Applied Biosystems) and 4 μl of the sample was mixed with 0.2 μl of 600-bp standard (Beckmann Coulter) and Sample Loading Solution (Beckmann Coulter) to a total volume of 30 μl.

Northern Blot:

Article Title: Microform-related community patterns of methane-cycling microbes in boreal Sphagnum bogs are site specific.
Article Snippet: Each 50-μl PCR reaction contained 0.5 μM of each primer, 200 μM of dNTPs and 1 U of DNA polymerase (Biotools B & M Labs S.A., Madrid, Spain) in 1 × reaction buffer and 1 μl of template DNA. .. Thermal cycling was started by initial denaturation at 94◦C for 3 min, followed by 40 cycles of denaturation at 94◦C for 30 s, annealing at 55◦C for 45 s, extension at 72◦C for 30 s and a final extension at 72◦C for 5 min. Products were digested for T-RFLP analysis with MboI (3 U, Promega) and HhaI (3 U, Promega) in a volume of 20 μl for 6 h. The enzymes were selected for optimal separation of methanogens common in northern peatlands based on previous sequence data from 11 peatlands (Martı́ et al. 2015). .. Digested products were ethanol precipitated, dissolved in 10 μl of Hi-Di formamide (Applied Biosystems) and 4 μl of the sample was mixed with 0.2 μl of 600-bp standard (Beckmann Coulter) and Sample Loading Solution (Beckmann Coulter) to a total volume of 30 μl.

Sequencing:

Article Title: Microform-related community patterns of methane-cycling microbes in boreal Sphagnum bogs are site specific.
Article Snippet: Each 50-μl PCR reaction contained 0.5 μM of each primer, 200 μM of dNTPs and 1 U of DNA polymerase (Biotools B & M Labs S.A., Madrid, Spain) in 1 × reaction buffer and 1 μl of template DNA. .. Thermal cycling was started by initial denaturation at 94◦C for 3 min, followed by 40 cycles of denaturation at 94◦C for 30 s, annealing at 55◦C for 45 s, extension at 72◦C for 30 s and a final extension at 72◦C for 5 min. Products were digested for T-RFLP analysis with MboI (3 U, Promega) and HhaI (3 U, Promega) in a volume of 20 μl for 6 h. The enzymes were selected for optimal separation of methanogens common in northern peatlands based on previous sequence data from 11 peatlands (Martı́ et al. 2015). .. Digested products were ethanol precipitated, dissolved in 10 μl of Hi-Di formamide (Applied Biosystems) and 4 μl of the sample was mixed with 0.2 μl of 600-bp standard (Beckmann Coulter) and Sample Loading Solution (Beckmann Coulter) to a total volume of 30 μl.

Polymerase Chain Reaction:

Article Title: Herbivory and Soil Water Availability Induce Changes in Arbuscular Mycorrhizal Fungal Abundance and Composition.
Article Snippet: We tested the prediction that abundance and composition of arbuscular mycorrhizal fungi (AMF) in Ipomopsis aggregata roots and soils are influenced by ungulate herbivory and drought conditions by examining the effects in a field setting over two years.. We used a multi-metric approach to quantify AMF root colonization, AMF reproduction, and AMF community composition in roots and soils.. We incorporated complimentary community characterization assays by morphologically identifying spores from trap cultures and the use of terminal restriction fragment length polymorphism (T-RFLP) fingerprinting.

Article Title: A Founder Mutation in PET100 Causes Isolated Complex IV Deficiency in Lebanese Individuals with Leigh Syndrome
Article Snippet: .. RFLP Analysis PCR product amplified by forward primer 5′-gccagacccgttttctattg-3′ and reverse primer 5′-aacccactcctgtgtccact-3′ was digested for 5 hr with MboI (Promega) as per manufacturer’s protocol and resolved on 2% agarose gels. .. Sequenom Genotyping Assay A specifically designed multiplexed MALDI-TOF mass spectrometry (Sequenom) assay was used to genotype 81 unrelated Lebanese individuals for the c.3G>C PET100 mutation.

Article Title: Long-term legacies and partial recovery of mycorrhizal communities after invasive plant removal
Article Snippet: Invasive plants can have strong impacts on native communities, which have prompted intense efforts at invasive removal.. However, relatively little is known about how native communities will reassemble after a dominant invader has been removed from the system.. Legacy effects of invasive plants on soil microbial communities may alter native plant community reassembly long after the invader is gone.

Electrophoresis:

Article Title: Herbivory and Soil Water Availability Induce Changes in Arbuscular Mycorrhizal Fungal Abundance and Composition.
Article Snippet: We tested the prediction that abundance and composition of arbuscular mycorrhizal fungi (AMF) in Ipomopsis aggregata roots and soils are influenced by ungulate herbivory and drought conditions by examining the effects in a field setting over two years.. We used a multi-metric approach to quantify AMF root colonization, AMF reproduction, and AMF community composition in roots and soils.. We incorporated complimentary community characterization assays by morphologically identifying spores from trap cultures and the use of terminal restriction fragment length polymorphism (T-RFLP) fingerprinting.

Article Title: Long-term legacies and partial recovery of mycorrhizal communities after invasive plant removal
Article Snippet: Invasive plants can have strong impacts on native communities, which have prompted intense efforts at invasive removal.. However, relatively little is known about how native communities will reassemble after a dominant invader has been removed from the system.. Legacy effects of invasive plants on soil microbial communities may alter native plant community reassembly long after the invader is gone.

Amplification:

Article Title: A Founder Mutation in PET100 Causes Isolated Complex IV Deficiency in Lebanese Individuals with Leigh Syndrome
Article Snippet: .. RFLP Analysis PCR product amplified by forward primer 5′-gccagacccgttttctattg-3′ and reverse primer 5′-aacccactcctgtgtccact-3′ was digested for 5 hr with MboI (Promega) as per manufacturer’s protocol and resolved on 2% agarose gels. .. Sequenom Genotyping Assay A specifically designed multiplexed MALDI-TOF mass spectrometry (Sequenom) assay was used to genotype 81 unrelated Lebanese individuals for the c.3G>C PET100 mutation.

other:

Article Title: Fungi in the future: interannual variation and effects of atmospheric change on arbuscular mycorrhizal fungal communities
Article Snippet: A further digestion using AluI or MboI (Promega), or additional sequencing, was performed if sequence analysis revealed that further differentiation of the initial RFLP types was required to confidently assign molecular operational taxonomic units (MOTUs).



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https://www.bioz.com/product/bamhi-digested+lambda+dash+ii+vector+arms/10__1046_slash_j__1529___8817__1998__340477__x-53-86-98
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