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bamhi  (New England Biolabs)


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    Structured Review

    New England Biolabs bamhi
    Bamhi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 13724 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bamhi-digested+lambda+dash+ii+vector+arms/BamHI/custom%40r0136%4016478484
    Average 99 stars, based on 13724 article reviews
    bamhi - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Cp36 serine recombinase as a new tool for zebrafish transgenesis
    Article Snippet: .. For the N-terminal vector, a TagRFPT sequence codon-optimized for expression in zebrafish amplified from pCS2-TagRFPT.zf1, a gift from Harold Burgess (Addgene plasmid # 61390) ( ) with Q5 polymerase (NEB) without the stop codon and adding a sequence encoding a 7-amino acid glycine-serine linker, including a BamHI site, at the C-terminal end, was cloned into the SalI site of Tol2- mfap4 (Addgene plasmid # 232189) ( ) by HiFi cloning (NEB). .. For the C-terminal vector, the TagRFPT sequence was amplified with the stop codon and adding a sequence encoding a 7-amino acid glycine-serine linker, including a BamHI site, at the N-terminal end and cloned into the SalI site of Tol2- mfap4 by HiFi cloning (NEB).

    Article Title: Human Histone Fragments Display Antibacterial Properties against Pseudomonas aeruginosa
    Article Snippet: .. Plasmid PAT28 was isolated using the QIAprep Spin Miniprep Kit (Qiagen, 250 preps) and digested with the restriction enzyme BamHI (New England Biolabs) according to the manufacturer’s protocol. .. The digested plasmid DNA was subsequently purified using the QIAquick PCR Purification Kit (Qiagen).

    Article Title: A chemoinformatics-guided platform for efficient discovery of RNA-binding small molecules: Proof-of-concept for myotonic dystrophy type 1
    Article Snippet: The DNA template for r(CUG) 42 was obtained from kanamycin-resistant pCTG109 vector previously cloned from a pCNA plasmid containing the repeat expansion sequence. .. The plasmid was linearized using BamHI restriction enzyme (New England Biolabs, Ipswich, MA, USA). ..

    Article Title: Identification of Multiple PlOSCs Involved in the Biosynthesis Pathway of Triterpenoids in Paeonia lactiflora
    Article Snippet: .. Amplicons were digested with BamHI restriction enzyme (New England Biolab, Ipswich, MA, USA) and inserted into the expression vector, pESC-Trp, with a pEASY-Uni Seamless Cloning and Assembly Kit (TransGen, Beijing, China). .. The confirmed recombinant plasmids were extracted and introduced into the ERG7-deficient yeast mutant (Saccharomyces cerevisiae, ATCC, cell line number: 4021900, -ERG7, -Trp) using the Frozen-EZ Yeast Transformation IITM Kit (Zymo Research, Irvine, CA, USA) according to the technical manual.

    Sequencing:

    Article Title: Cp36 serine recombinase as a new tool for zebrafish transgenesis
    Article Snippet: .. For the N-terminal vector, a TagRFPT sequence codon-optimized for expression in zebrafish amplified from pCS2-TagRFPT.zf1, a gift from Harold Burgess (Addgene plasmid # 61390) ( ) with Q5 polymerase (NEB) without the stop codon and adding a sequence encoding a 7-amino acid glycine-serine linker, including a BamHI site, at the C-terminal end, was cloned into the SalI site of Tol2- mfap4 (Addgene plasmid # 232189) ( ) by HiFi cloning (NEB). .. For the C-terminal vector, the TagRFPT sequence was amplified with the stop codon and adding a sequence encoding a 7-amino acid glycine-serine linker, including a BamHI site, at the N-terminal end and cloned into the SalI site of Tol2- mfap4 by HiFi cloning (NEB).

    Expressing:

    Article Title: Cp36 serine recombinase as a new tool for zebrafish transgenesis
    Article Snippet: .. For the N-terminal vector, a TagRFPT sequence codon-optimized for expression in zebrafish amplified from pCS2-TagRFPT.zf1, a gift from Harold Burgess (Addgene plasmid # 61390) ( ) with Q5 polymerase (NEB) without the stop codon and adding a sequence encoding a 7-amino acid glycine-serine linker, including a BamHI site, at the C-terminal end, was cloned into the SalI site of Tol2- mfap4 (Addgene plasmid # 232189) ( ) by HiFi cloning (NEB). .. For the C-terminal vector, the TagRFPT sequence was amplified with the stop codon and adding a sequence encoding a 7-amino acid glycine-serine linker, including a BamHI site, at the N-terminal end and cloned into the SalI site of Tol2- mfap4 by HiFi cloning (NEB).

    Article Title: Identification of Multiple PlOSCs Involved in the Biosynthesis Pathway of Triterpenoids in Paeonia lactiflora
    Article Snippet: .. Amplicons were digested with BamHI restriction enzyme (New England Biolab, Ipswich, MA, USA) and inserted into the expression vector, pESC-Trp, with a pEASY-Uni Seamless Cloning and Assembly Kit (TransGen, Beijing, China). .. The confirmed recombinant plasmids were extracted and introduced into the ERG7-deficient yeast mutant (Saccharomyces cerevisiae, ATCC, cell line number: 4021900, -ERG7, -Trp) using the Frozen-EZ Yeast Transformation IITM Kit (Zymo Research, Irvine, CA, USA) according to the technical manual.

    Amplification:

    Article Title: Cp36 serine recombinase as a new tool for zebrafish transgenesis
    Article Snippet: .. For the N-terminal vector, a TagRFPT sequence codon-optimized for expression in zebrafish amplified from pCS2-TagRFPT.zf1, a gift from Harold Burgess (Addgene plasmid # 61390) ( ) with Q5 polymerase (NEB) without the stop codon and adding a sequence encoding a 7-amino acid glycine-serine linker, including a BamHI site, at the C-terminal end, was cloned into the SalI site of Tol2- mfap4 (Addgene plasmid # 232189) ( ) by HiFi cloning (NEB). .. For the C-terminal vector, the TagRFPT sequence was amplified with the stop codon and adding a sequence encoding a 7-amino acid glycine-serine linker, including a BamHI site, at the N-terminal end and cloned into the SalI site of Tol2- mfap4 by HiFi cloning (NEB).

    Clone Assay:

    Article Title: Cp36 serine recombinase as a new tool for zebrafish transgenesis
    Article Snippet: .. For the N-terminal vector, a TagRFPT sequence codon-optimized for expression in zebrafish amplified from pCS2-TagRFPT.zf1, a gift from Harold Burgess (Addgene plasmid # 61390) ( ) with Q5 polymerase (NEB) without the stop codon and adding a sequence encoding a 7-amino acid glycine-serine linker, including a BamHI site, at the C-terminal end, was cloned into the SalI site of Tol2- mfap4 (Addgene plasmid # 232189) ( ) by HiFi cloning (NEB). .. For the C-terminal vector, the TagRFPT sequence was amplified with the stop codon and adding a sequence encoding a 7-amino acid glycine-serine linker, including a BamHI site, at the N-terminal end and cloned into the SalI site of Tol2- mfap4 by HiFi cloning (NEB).

    Cloning:

    Article Title: Cp36 serine recombinase as a new tool for zebrafish transgenesis
    Article Snippet: .. For the N-terminal vector, a TagRFPT sequence codon-optimized for expression in zebrafish amplified from pCS2-TagRFPT.zf1, a gift from Harold Burgess (Addgene plasmid # 61390) ( ) with Q5 polymerase (NEB) without the stop codon and adding a sequence encoding a 7-amino acid glycine-serine linker, including a BamHI site, at the C-terminal end, was cloned into the SalI site of Tol2- mfap4 (Addgene plasmid # 232189) ( ) by HiFi cloning (NEB). .. For the C-terminal vector, the TagRFPT sequence was amplified with the stop codon and adding a sequence encoding a 7-amino acid glycine-serine linker, including a BamHI site, at the N-terminal end and cloned into the SalI site of Tol2- mfap4 by HiFi cloning (NEB).

    Article Title: Identification of Multiple PlOSCs Involved in the Biosynthesis Pathway of Triterpenoids in Paeonia lactiflora
    Article Snippet: .. Amplicons were digested with BamHI restriction enzyme (New England Biolab, Ipswich, MA, USA) and inserted into the expression vector, pESC-Trp, with a pEASY-Uni Seamless Cloning and Assembly Kit (TransGen, Beijing, China). .. The confirmed recombinant plasmids were extracted and introduced into the ERG7-deficient yeast mutant (Saccharomyces cerevisiae, ATCC, cell line number: 4021900, -ERG7, -Trp) using the Frozen-EZ Yeast Transformation IITM Kit (Zymo Research, Irvine, CA, USA) according to the technical manual.

    Isolation:

    Article Title: Human Histone Fragments Display Antibacterial Properties against Pseudomonas aeruginosa
    Article Snippet: .. Plasmid PAT28 was isolated using the QIAprep Spin Miniprep Kit (Qiagen, 250 preps) and digested with the restriction enzyme BamHI (New England Biolabs) according to the manufacturer’s protocol. .. The digested plasmid DNA was subsequently purified using the QIAquick PCR Purification Kit (Qiagen).

    other:

    Article Title: Hemin activates interleukin-17 signaling and CCAAT/enhancer-binding protein beta to promote neuroinflammation and blood-brain barrier disruption.
    Article Snippet: Heme overload, a feature of hemolytic conditions and cellular injury, can initiate oxidative stress and inflammatory cascades that perturb neurovascular homeostasis.. However, the molecular mechanisms by which peripheral heme exposure disrupts blood–brain barrier (BBB) integrity remain incompletely defined.. Here, we investigated key molecular mediators linking peripheral hemin challenge to neurovascular injury.



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