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Promega pci vector backbone
Pci Vector Backbone, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/backbone+vector/pci+vector+backbone/us11066656-1449-8-11
Average 90 stars, based on 1 article reviews
pci vector backbone - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Expressing:

Article Title: Combinations and methods for subcutaneous administration of immune globulin and hyaluronidase
Article Snippet: Exemplary of a vector for mammalian cell expression is the HZ24 expression vector. .. The HZ24 expression vector was derived from the pCI vector backbone (Promega). ..

Article Title: Soluble glycosaminoglycanases and methods of preparing and using soluble glycosaminoglycanases
Article Snippet: Generation of a Human sHASEGP Expression Vector A sHASEGP without an epitope tag was generated by cloning into a bicistronic expression cassette, HZ24 (SEQ ID NO 51). .. The HZ24 plasmid vector for expression of sHASEGP comprises a pCI vector backbone (Promega), DNA sequence encoding amino acids 1-482 of human PH20 hyaluronidase, an internal ribosomal entry site (IRES) from the ECMV virus (Clontech), and the mouse dihydrofolate reductase (DHFR) gene. ..

Article Title: Modified hyaluronidases and uses in treating hyaluronan-associated diseases and conditions
Article Snippet: Exemplary of a vector for mammalian cell expression is the HZ24 expression vector. .. The HZ24 expression vector was derived from the pCI vector backbone (Promega). ..

Article Title: Methods of expressing products in mammalian cells
Article Snippet: .. The selectable marker genes can be inserted into a vector backbone, such as a pCI vector backbone (Promega), that contains promoter and enhancer elements, such as a CMV promoter and a synthetic intron, to maximize expression of a recombinant product. .. To introduce the plasmids into the host cells, the vector can be inserted into the cells using, for example, cationic-lipid-based transfections with Free-style Max (Invitrogen) for 48 hours in CHO-S or CHO-K1 cell lines, with a few different transfection conditions performed in parallel.

Article Title: Combination therapy with an anti-hyaluronan agent and therapeutic agent
Article Snippet: Exemplary of a vector for mammalian cell expression is the HZ24 expression vector. .. The HZ24 expression vector was derived from the pCI vector backbone (Promega). ..

Article Title: PH20 polypeptide variants, formulations and uses thereof
Article Snippet: The HZ24 expression vector was derived from the pCI vector backbone (Promega). .. The HZ24 expression vector was derived from the pCI vector backbone (Promega). .. The expression vector also has an internal ribosome entry site (IRES) from the ECMV virus (Clontech) and the mouse dihydrofolate reductase (DHFR) gene.

Article Title: Combination therapy with a hyaluronan-degrading enzyme and an immune checkpoint inhibitor
Article Snippet: .. The HZ24 expression vector was derived from the pCI vector backbone (Promega). ..

Plasmid Preparation:

Article Title: Combinations and methods for subcutaneous administration of immune globulin and hyaluronidase
Article Snippet: Exemplary of a vector for mammalian cell expression is the HZ24 expression vector. .. The HZ24 expression vector was derived from the pCI vector backbone (Promega). ..

Article Title: Soluble glycosaminoglycanases and methods of preparing and using soluble glycosaminoglycanases
Article Snippet: Generation of a Human sHASEGP Expression Vector A sHASEGP without an epitope tag was generated by cloning into a bicistronic expression cassette, HZ24 (SEQ ID NO 51). .. The HZ24 plasmid vector for expression of sHASEGP comprises a pCI vector backbone (Promega), DNA sequence encoding amino acids 1-482 of human PH20 hyaluronidase, an internal ribosomal entry site (IRES) from the ECMV virus (Clontech), and the mouse dihydrofolate reductase (DHFR) gene. ..

Article Title: Modified hyaluronidases and uses in treating hyaluronan-associated diseases and conditions
Article Snippet: Exemplary of a vector for mammalian cell expression is the HZ24 expression vector. .. The HZ24 expression vector was derived from the pCI vector backbone (Promega). ..

Article Title: Methods of expressing products in mammalian cells
Article Snippet: .. The selectable marker genes can be inserted into a vector backbone, such as a pCI vector backbone (Promega), that contains promoter and enhancer elements, such as a CMV promoter and a synthetic intron, to maximize expression of a recombinant product. .. To introduce the plasmids into the host cells, the vector can be inserted into the cells using, for example, cationic-lipid-based transfections with Free-style Max (Invitrogen) for 48 hours in CHO-S or CHO-K1 cell lines, with a few different transfection conditions performed in parallel.

Article Title: Combination therapy with an anti-hyaluronan agent and therapeutic agent
Article Snippet: Exemplary of a vector for mammalian cell expression is the HZ24 expression vector. .. The HZ24 expression vector was derived from the pCI vector backbone (Promega). ..

Article Title: PH20 polypeptide variants, formulations and uses thereof
Article Snippet: The HZ24 expression vector was derived from the pCI vector backbone (Promega). .. The HZ24 expression vector was derived from the pCI vector backbone (Promega). .. The expression vector also has an internal ribosome entry site (IRES) from the ECMV virus (Clontech) and the mouse dihydrofolate reductase (DHFR) gene.

Article Title: Combination therapy with a hyaluronan-degrading enzyme and an immune checkpoint inhibitor
Article Snippet: .. The HZ24 expression vector was derived from the pCI vector backbone (Promega). ..

Derivative Assay:

Article Title: Combinations and methods for subcutaneous administration of immune globulin and hyaluronidase
Article Snippet: Exemplary of a vector for mammalian cell expression is the HZ24 expression vector. .. The HZ24 expression vector was derived from the pCI vector backbone (Promega). ..

Article Title: Modified hyaluronidases and uses in treating hyaluronan-associated diseases and conditions
Article Snippet: Exemplary of a vector for mammalian cell expression is the HZ24 expression vector. .. The HZ24 expression vector was derived from the pCI vector backbone (Promega). ..

Article Title: Combination therapy with an anti-hyaluronan agent and therapeutic agent
Article Snippet: Exemplary of a vector for mammalian cell expression is the HZ24 expression vector. .. The HZ24 expression vector was derived from the pCI vector backbone (Promega). ..

Article Title: PH20 polypeptide variants, formulations and uses thereof
Article Snippet: The HZ24 expression vector was derived from the pCI vector backbone (Promega). .. The HZ24 expression vector was derived from the pCI vector backbone (Promega). .. The expression vector also has an internal ribosome entry site (IRES) from the ECMV virus (Clontech) and the mouse dihydrofolate reductase (DHFR) gene.

Article Title: Combination therapy with a hyaluronan-degrading enzyme and an immune checkpoint inhibitor
Article Snippet: .. The HZ24 expression vector was derived from the pCI vector backbone (Promega). ..

other:

Article Title: Compositions of adenosine deaminase-2 (ADA2), variants thereof and methods of using same
Article Snippet: The expression vector also has an internal ribosome entry site (IRES) from the ECMV virus (Clontech) and the mouse dihydrofolate reductase (DHFR) gene.

Sequencing:

Article Title: Soluble glycosaminoglycanases and methods of preparing and using soluble glycosaminoglycanases
Article Snippet: Generation of a Human sHASEGP Expression Vector A sHASEGP without an epitope tag was generated by cloning into a bicistronic expression cassette, HZ24 (SEQ ID NO 51). .. The HZ24 plasmid vector for expression of sHASEGP comprises a pCI vector backbone (Promega), DNA sequence encoding amino acids 1-482 of human PH20 hyaluronidase, an internal ribosomal entry site (IRES) from the ECMV virus (Clontech), and the mouse dihydrofolate reductase (DHFR) gene. ..

Virus:

Article Title: Soluble glycosaminoglycanases and methods of preparing and using soluble glycosaminoglycanases
Article Snippet: Generation of a Human sHASEGP Expression Vector A sHASEGP without an epitope tag was generated by cloning into a bicistronic expression cassette, HZ24 (SEQ ID NO 51). .. The HZ24 plasmid vector for expression of sHASEGP comprises a pCI vector backbone (Promega), DNA sequence encoding amino acids 1-482 of human PH20 hyaluronidase, an internal ribosomal entry site (IRES) from the ECMV virus (Clontech), and the mouse dihydrofolate reductase (DHFR) gene. ..

Marker:

Article Title: Methods of expressing products in mammalian cells
Article Snippet: .. The selectable marker genes can be inserted into a vector backbone, such as a pCI vector backbone (Promega), that contains promoter and enhancer elements, such as a CMV promoter and a synthetic intron, to maximize expression of a recombinant product. .. To introduce the plasmids into the host cells, the vector can be inserted into the cells using, for example, cationic-lipid-based transfections with Free-style Max (Invitrogen) for 48 hours in CHO-S or CHO-K1 cell lines, with a few different transfection conditions performed in parallel.

Recombinant:

Article Title: Methods of expressing products in mammalian cells
Article Snippet: .. The selectable marker genes can be inserted into a vector backbone, such as a pCI vector backbone (Promega), that contains promoter and enhancer elements, such as a CMV promoter and a synthetic intron, to maximize expression of a recombinant product. .. To introduce the plasmids into the host cells, the vector can be inserted into the cells using, for example, cationic-lipid-based transfections with Free-style Max (Invitrogen) for 48 hours in CHO-S or CHO-K1 cell lines, with a few different transfection conditions performed in parallel.



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