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plvx ireszsgreen backbone  (TaKaRa)


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    Structured Review

    TaKaRa plvx ireszsgreen backbone
    Plvx Ireszsgreen Backbone, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 407 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/backbone+vector/pLVX-IRES-ZsGreen1+Vector/pm41957014-173-10-12
    Average 96 stars, based on 407 article reviews
    plvx ireszsgreen backbone - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Lipid asymmetry disruption by XKR8 orchestrates neutrophil extracellular trap formation and inhibits fungal infection.
    Article Snippet: .. The cDNA of Xkr8 and its 2DA, S/T-3A, D30A and W45A mutants were cloned into pLVX-IRES-ZsGreen1 (Clontech, Cat. 632187). ..

    Generated:

    Article Title: SIRT7 safeguards ERα proteostasis via deacetylation-dependent degradation of unliganded and misfolded receptors
    Article Snippet: .. Plasmids were generated using PCR amplification (YEASEN, 10154ES, China) followed by cloning with the One Step Cloning Kit (Vazyme Biotech, C112) into the pcDNA3.1 vector (Thermo Fisher Scientific) or the pLVX-IRES-ZsGreen1 and pLVX-TRE3G-IRES lentiviral vector (Takara). .. All constructs were verified by DNA sequencing (Beijing Tsingke Biotech).

    Polymerase Chain Reaction:

    Article Title: SIRT7 safeguards ERα proteostasis via deacetylation-dependent degradation of unliganded and misfolded receptors
    Article Snippet: .. Plasmids were generated using PCR amplification (YEASEN, 10154ES, China) followed by cloning with the One Step Cloning Kit (Vazyme Biotech, C112) into the pcDNA3.1 vector (Thermo Fisher Scientific) or the pLVX-IRES-ZsGreen1 and pLVX-TRE3G-IRES lentiviral vector (Takara). .. All constructs were verified by DNA sequencing (Beijing Tsingke Biotech).

    Article Title: Decorin facilitates T cell-mediated antitumor immunity and augments the efficacy of anti-PD1 immunotherapy.
    Article Snippet: .. Plasmids and stable cell line construction The mouse Dcn gene was PCR amplified and subcloned into the pLVX vector (632187, Clontech). ..

    Article Title: Decorin facilitates T cell-mediated antitumor immunity and augments the efficacy of anti-PD1 immunotherapy
    Article Snippet: .. The mouse Dcn gene was PCR amplified and subcloned into the pLVX vector (632187, Clontech). ..

    Amplification:

    Article Title: SIRT7 safeguards ERα proteostasis via deacetylation-dependent degradation of unliganded and misfolded receptors
    Article Snippet: .. Plasmids were generated using PCR amplification (YEASEN, 10154ES, China) followed by cloning with the One Step Cloning Kit (Vazyme Biotech, C112) into the pcDNA3.1 vector (Thermo Fisher Scientific) or the pLVX-IRES-ZsGreen1 and pLVX-TRE3G-IRES lentiviral vector (Takara). .. All constructs were verified by DNA sequencing (Beijing Tsingke Biotech).

    Article Title: Decorin facilitates T cell-mediated antitumor immunity and augments the efficacy of anti-PD1 immunotherapy.
    Article Snippet: .. Plasmids and stable cell line construction The mouse Dcn gene was PCR amplified and subcloned into the pLVX vector (632187, Clontech). ..

    Article Title: Decorin facilitates T cell-mediated antitumor immunity and augments the efficacy of anti-PD1 immunotherapy
    Article Snippet: .. The mouse Dcn gene was PCR amplified and subcloned into the pLVX vector (632187, Clontech). ..

    Cloning:

    Article Title: SIRT7 safeguards ERα proteostasis via deacetylation-dependent degradation of unliganded and misfolded receptors
    Article Snippet: .. Plasmids were generated using PCR amplification (YEASEN, 10154ES, China) followed by cloning with the One Step Cloning Kit (Vazyme Biotech, C112) into the pcDNA3.1 vector (Thermo Fisher Scientific) or the pLVX-IRES-ZsGreen1 and pLVX-TRE3G-IRES lentiviral vector (Takara). .. All constructs were verified by DNA sequencing (Beijing Tsingke Biotech).

    Plasmid Preparation:

    Article Title: SIRT7 safeguards ERα proteostasis via deacetylation-dependent degradation of unliganded and misfolded receptors
    Article Snippet: .. Plasmids were generated using PCR amplification (YEASEN, 10154ES, China) followed by cloning with the One Step Cloning Kit (Vazyme Biotech, C112) into the pcDNA3.1 vector (Thermo Fisher Scientific) or the pLVX-IRES-ZsGreen1 and pLVX-TRE3G-IRES lentiviral vector (Takara). .. All constructs were verified by DNA sequencing (Beijing Tsingke Biotech).

    Article Title: Decorin facilitates T cell-mediated antitumor immunity and augments the efficacy of anti-PD1 immunotherapy.
    Article Snippet: .. Plasmids and stable cell line construction The mouse Dcn gene was PCR amplified and subcloned into the pLVX vector (632187, Clontech). ..

    Article Title: Decorin facilitates T cell-mediated antitumor immunity and augments the efficacy of anti-PD1 immunotherapy
    Article Snippet: .. The mouse Dcn gene was PCR amplified and subcloned into the pLVX vector (632187, Clontech). ..

    Stable Transfection:

    Article Title: Decorin facilitates T cell-mediated antitumor immunity and augments the efficacy of anti-PD1 immunotherapy.
    Article Snippet: .. Plasmids and stable cell line construction The mouse Dcn gene was PCR amplified and subcloned into the pLVX vector (632187, Clontech). ..



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