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bbsi digested pxr003 vector  (Addgene inc)


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    Structured Review

    Addgene inc bbsi digested pxr003 vector
    Bbsi Digested Pxr003 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/backbone+vector/pXR003%3A+CasRx+gRNA+cloning+backbone+(Plasmid+%23109053)/pm41928225-94-9-12
    Average 95 stars, based on 71 article reviews
    bbsi digested pxr003 vector - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Cloning:

    Article Title: RSRC2 is a novel RNA-binding protein that safeguards mitotic fidelity by interacting with the lncRNA C1QTNF1-AS1
    Article Snippet: .. Five micrograms of pxR003 CasRx gRNA cloning backbone (pUC19; 109053, Addgene) was linearized with 10 Units of BbsI restriction enzyme (R3539, NEB) at 37°C for 4 h. BbsI was then heat-inactivated at 65°C for 20 min. .. The linearized plasmid was run on 1% agarose gel, and the corresponding band was cut and extracted using QIAquick ® Gel Extraction Kit (28 704, QIAGEN).

    Article Title: LncRNA CISTR-ACT regulates cell size in human and mouse by guiding FOSL2.
    Article Snippet: .. To generate pXR003_puroR (Addgene #219819), we amplified the puromycin resistance cassette from BPK1520_puroR (Addgene #173901) and added to pXR003 (Addgene #109053) by NheI-HF restriction cloning. .. To generate a multi-gRNA array plasmid which also encodes CasRx, we added a second direct repeat to pLentiRNACRISPR_005-hU6DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE (Addgene #138147) to convert the sgRNA backbone to a pre-gRNA backbone.

    Article Title: RSRC2 is a novel RNA-binding protein that safeguards mitotic fidelity by interacting with the lncRNA C1QTNF1-AS1.
    Article Snippet: .. Five micrograms of pxR003 CasRx gRNA cloning backbone (pUC19; 109053, Addgene) was linearized with 10 Units of BbsI restriction enzyme (R3539, NEB) at 37 ◦C for 4 h. BbsI was then heat-inactivated at 65 ◦C for 20 min. .. The linearized plasmid was run on 1% agarose gel, and the corresponding band was cut and extracted using QIAquick ® Gel Extraction Kit (28 704, QIAGEN).

    Article Title: Programmable RNA N 6 ,2´-O-Dimethyladenosine Editing.
    Article Snippet: We amplified these plasmids using the DH5 α (Tolobio) competent cells. .. The dCasRx (Addgene plasmid no. 109050) and CasRx gRNA cloning backbone (Addgene plasmid no. 109053) were purchased from Addgene. ..

    Generated:

    Article Title: Integrative high-throughput studies to develop novel targets and drugs for the treatment of advanced prostate cancer
    Article Snippet: .. Three guide RNAs (gRNA) for each target, including RB1, E2F1, CDC20, RRM2, and DTL, were designed, generated, and cloned into CRSIPR-Cas13 corresponding gRNA backbone (Addgene, 109053). .. The gRNA plasmid and CRISPR-Cas13 plasmid (Addgene, 109049) were co-transfected into prostate cancer cells with Lipomaster 3000 Transfection Reagent (Vazyme, TL301-01) as we described previously.

    Clone Assay:

    Article Title: Integrative high-throughput studies to develop novel targets and drugs for the treatment of advanced prostate cancer
    Article Snippet: .. Three guide RNAs (gRNA) for each target, including RB1, E2F1, CDC20, RRM2, and DTL, were designed, generated, and cloned into CRSIPR-Cas13 corresponding gRNA backbone (Addgene, 109053). .. The gRNA plasmid and CRISPR-Cas13 plasmid (Addgene, 109049) were co-transfected into prostate cancer cells with Lipomaster 3000 Transfection Reagent (Vazyme, TL301-01) as we described previously.

    Article Title: Programmable RNA N 6 ,2´-O-Dimethyladenosine Editing.
    Article Snippet: Then PCIF1 or PCIF1N553A was fused to the N- or C-terminus of the dCasRx fragment through homologous recombination using the MultiF Seamless Assembly Mix (Abclonal). .. The CasRx gRNAs were golden-gate cloned into the pXR003-CasRx-gRNA (Addgene plasmid no. 109053) with BbsI-HF restriction enzyme (NEB) and T4 DNA ligase (NEB), which had constitutive gRNA expression driven by the hU6 promoter. .. We amplified these plasmids using the DH5 α (Tolobio) competent cells.

    Amplification:

    Article Title: LncRNA CISTR-ACT regulates cell size in human and mouse by guiding FOSL2.
    Article Snippet: .. To generate pXR003_puroR (Addgene #219819), we amplified the puromycin resistance cassette from BPK1520_puroR (Addgene #173901) and added to pXR003 (Addgene #109053) by NheI-HF restriction cloning. .. To generate a multi-gRNA array plasmid which also encodes CasRx, we added a second direct repeat to pLentiRNACRISPR_005-hU6DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE (Addgene #138147) to convert the sgRNA backbone to a pre-gRNA backbone.

    Plasmid Preparation:

    Article Title: Functional sQTLs regulating PTK2B exon 31 splicing uncover an RNA-dependent modulation of its kinase activity and cellular phenotype.
    Article Snippet: .. The resulting doublestranded oligonucleotides were then ligated into the BbsI-digested pXR003 vector (Addgene plasmid #109053) [49]. ..

    Article Title: Programmable RNA N 6 ,2´-O-Dimethyladenosine Editing.
    Article Snippet: Then PCIF1 or PCIF1N553A was fused to the N- or C-terminus of the dCasRx fragment through homologous recombination using the MultiF Seamless Assembly Mix (Abclonal). .. The CasRx gRNAs were golden-gate cloned into the pXR003-CasRx-gRNA (Addgene plasmid no. 109053) with BbsI-HF restriction enzyme (NEB) and T4 DNA ligase (NEB), which had constitutive gRNA expression driven by the hU6 promoter. .. We amplified these plasmids using the DH5 α (Tolobio) competent cells.

    Article Title: Programmable RNA N 6 ,2´-O-Dimethyladenosine Editing.
    Article Snippet: We amplified these plasmids using the DH5 α (Tolobio) competent cells. .. The dCasRx (Addgene plasmid no. 109050) and CasRx gRNA cloning backbone (Addgene plasmid no. 109053) were purchased from Addgene. ..

    Expressing:

    Article Title: Programmable RNA N 6 ,2´-O-Dimethyladenosine Editing.
    Article Snippet: Then PCIF1 or PCIF1N553A was fused to the N- or C-terminus of the dCasRx fragment through homologous recombination using the MultiF Seamless Assembly Mix (Abclonal). .. The CasRx gRNAs were golden-gate cloned into the pXR003-CasRx-gRNA (Addgene plasmid no. 109053) with BbsI-HF restriction enzyme (NEB) and T4 DNA ligase (NEB), which had constitutive gRNA expression driven by the hU6 promoter. .. We amplified these plasmids using the DH5 α (Tolobio) competent cells.



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