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aav2 vector backbone  (Addgene inc)


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    Structured Review

    Addgene inc aav2 vector backbone
    Aav2 Vector Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 278 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/backbone+vector/pAAV-CAG-tdTomato+(codon+diversified)+(Plasmid+%2359462)/pm41856998-209-6-10
    Average 96 stars, based on 278 article reviews
    aav2 vector backbone - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Bioprocessing:

    Article Title: Developmental Profiling of Structural and Functional Maturation in Mouse Corpus Callosum
    Article Snippet: .. Adeno‐Associated Viral (AAV) particles expressing the red fluorescent tdTomato protein (AAV‐CAG‐tdTomato (codon diversified), Addgene Cat # 59462‐AAV1, gift from Edward Boyden, RRID:Addgene_59,462, 1:3 dilution with ddH 2 O from the original titer of ≥ 5 × 10 12 vg/mL) was injected into the right sensory‐motor cortex at 300–400 μm depth (AP coordinates from bregma in mm: AP +0.2/1.1, −1.0/1.1, 400 nL/injection site at 50 nL/min speed using Nanoliter 2010, World Precision Instruments). ..

    Article Title: Hyperinnervation inhibits organ-level regeneration in mammalian skin.
    Article Snippet: All mice were maintained in an AAALAC-accredited (Association for Assessment and Accreditation of Laboratory Animal Care) animal facility at Harvard University. .. pAAV-CAG-tdTomato was a gift from Edward Boyden (Addgene plasmid #59462) and was used as the control AAV plasmid. .. Human wounding experiments were conducted with informed consent under Mass General Brigham Institutional Review Board approval (#2022P000229).

    Article Title: Targeting of Itch by clomipramine or gene therapy improves cognitive defects related to Alzheimer’s disease
    Article Snippet: .. For preparation of recombinant AAV, mouse WT or T222A/S232A or K393R mutant Itch was subcloned from the constructs described previously in pAAV-CAG-tdTomato vector [(pAAV-CAG-tdTomato (codon diversified)], which was a gift from Edward Boyden (Addgene plasmid #59462; http://addgene.org/59462 ; RRID: Addgene_59462). ..

    Expressing:

    Article Title: Developmental Profiling of Structural and Functional Maturation in Mouse Corpus Callosum
    Article Snippet: .. Adeno‐Associated Viral (AAV) particles expressing the red fluorescent tdTomato protein (AAV‐CAG‐tdTomato (codon diversified), Addgene Cat # 59462‐AAV1, gift from Edward Boyden, RRID:Addgene_59,462, 1:3 dilution with ddH 2 O from the original titer of ≥ 5 × 10 12 vg/mL) was injected into the right sensory‐motor cortex at 300–400 μm depth (AP coordinates from bregma in mm: AP +0.2/1.1, −1.0/1.1, 400 nL/injection site at 50 nL/min speed using Nanoliter 2010, World Precision Instruments). ..

    Injection:

    Article Title: Developmental Profiling of Structural and Functional Maturation in Mouse Corpus Callosum
    Article Snippet: .. Adeno‐Associated Viral (AAV) particles expressing the red fluorescent tdTomato protein (AAV‐CAG‐tdTomato (codon diversified), Addgene Cat # 59462‐AAV1, gift from Edward Boyden, RRID:Addgene_59,462, 1:3 dilution with ddH 2 O from the original titer of ≥ 5 × 10 12 vg/mL) was injected into the right sensory‐motor cortex at 300–400 μm depth (AP coordinates from bregma in mm: AP +0.2/1.1, −1.0/1.1, 400 nL/injection site at 50 nL/min speed using Nanoliter 2010, World Precision Instruments). ..

    Virus:

    Article Title: A developmental switch in corticoclaustral signaling.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken anti-GFP Aves Labs Cat#: gfp1010; RRID: AB_2307313 Rabbit anti-Nr2f2 Abcam Cat#: ab211776; RRID: AB_2893028 Goat anti-Nurr1 R&D Systems Cat#: AF2156; RRID: AB_2153894 Goat anti-PV Swant Cat#: PVG213; RRID: AB_2313848 Rat anti-SST Millipore Cat#: MAB354; RRID: AB_2255365 Mouse anti-Tle4 Santa Cruz Biotechnology Cat#: sc365406; RRID: AB_10841582 Chicken anti-rat IgG Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21472, RRID: AB_2535875 Donkey anti-Chicken IgY Alexa Fluor 488 Thermo Fisher Scientific Cat#: A-78948; RRID: AB_2921070 Donkey anti-Chicken IgY Alexa Fluor 647 Thermo Fisher Scientific Cat#: A-78952; RRID: AB_2921074 Donkey anti-Goat IgG Alexa Fluor 555 Thermo Fisher Scientific Cat# A-21432, RRID: AB_2535853 Donkey anti-Goat IgG Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21447, RRID: AB_2535864 Donkey anti-mouse IgG Alexa Fluor 488 Thermo Fisher Scientific Cat# A-21202, RRID: AB_141607 Bacterial and virus strains pAAV-CAG-GFP Gift from Edward Boyden RRID: Addgene_37825-AAVrg pAAV-CAG-tdTomato Gift from Edward Boyden RRID: Addgene_59462-AAVrg pAAV-hSyn-hChR2(H134R)-EYFP Gift from Karl Deisseroth RRID: Addgene_26973-AAV1 pAAV-CaMKIIa-hChR2(H134R)-EYFP Gift from Karl Deisseroth RRID: Addgene_26969-AAV1 pAAV-hSyn-Cre-P2A-dTomato Gift from Rylan Larsen RRID: Addgene_107738-AAVrg pAAV-hSyn-DIO-hM4D(Gi)-mCherry Gift from Bryan Roth RRID: Addgene_44362-AAV5 Chemicals, peptides, and recombinant proteins Clozapine-n-oxide Enzo Life Sciences Cat#: BML-NS105 DiD stain Thermo Fisher Scientific Cat#: D7757 DiI stain Thermo Fisher Scientific Cat#: D3911 Experimental models: organisms/strains Mouse: Ai14 Jackson Laboratories RRID: IMSR_JAX: 007914 Mouse: C57BL/6J Jackson Laboratories RRID: IMSR_JAX: 000664 Mouse: PV-cre Jackson Laboratories RRID: IMSR_JAX:017320 Software and algorithms Illustrator Adobe RRID: SCR_010279 ImageJ (Fiji) National Institute of Health RRID: SCR_003070 MATLAB MathWorks RRID: SCR_001622 Prism Graphpad RRID: SCR_02798 Other Fiber optic cannula RWD Cat#: R-FOC-L200C-39NA Recording electrode (4 channels) Neuronexus Cat#: Q1x4-10mm-50-177-Q4 ll OPEN ACCESS Current Biology 36, 1–10.e1–e5, April 6, 2026 e1 Report .. Stereotaxic injection of viral vectors Adeno-associated viruses (AAVs) were purchased from Addgene (MA, USA) at titer ≥ 1×1012 vg/ml. pAAV-CaMKIIa-hChR2(H134R)EYFP was a gift from Karl Deisseroth (Addgene plasmid # 26969; http://n2t.net/addgene:26969; RRID:Addgene_26969).78 pAAVCAG-GFP was a gift from Edward Boyden (Addgene viral prep # 37825-AAVrg.T; RRID:Addgene_37825). pAAV-CAG-tdTomato (codon diversified) was a gift from Edward Boyden (viral prep # 59462-AAVrg; RRID:Addgene_59462). pAAV-hSyn-DIO-hM4D(Gi)mCherry was a gift from Bryan Roth (Addgene viral prep # 44362-AAV5); RRID:Addgene_44362).79 pAAV-hSyn-Cre-P2A-dTomato was a gift from Rylan Larsen (Addgene viral prep # 107738-AAVrg; RRID:Addgene_107738).

    Recombinant:

    Article Title: A developmental switch in corticoclaustral signaling.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken anti-GFP Aves Labs Cat#: gfp1010; RRID: AB_2307313 Rabbit anti-Nr2f2 Abcam Cat#: ab211776; RRID: AB_2893028 Goat anti-Nurr1 R&D Systems Cat#: AF2156; RRID: AB_2153894 Goat anti-PV Swant Cat#: PVG213; RRID: AB_2313848 Rat anti-SST Millipore Cat#: MAB354; RRID: AB_2255365 Mouse anti-Tle4 Santa Cruz Biotechnology Cat#: sc365406; RRID: AB_10841582 Chicken anti-rat IgG Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21472, RRID: AB_2535875 Donkey anti-Chicken IgY Alexa Fluor 488 Thermo Fisher Scientific Cat#: A-78948; RRID: AB_2921070 Donkey anti-Chicken IgY Alexa Fluor 647 Thermo Fisher Scientific Cat#: A-78952; RRID: AB_2921074 Donkey anti-Goat IgG Alexa Fluor 555 Thermo Fisher Scientific Cat# A-21432, RRID: AB_2535853 Donkey anti-Goat IgG Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21447, RRID: AB_2535864 Donkey anti-mouse IgG Alexa Fluor 488 Thermo Fisher Scientific Cat# A-21202, RRID: AB_141607 Bacterial and virus strains pAAV-CAG-GFP Gift from Edward Boyden RRID: Addgene_37825-AAVrg pAAV-CAG-tdTomato Gift from Edward Boyden RRID: Addgene_59462-AAVrg pAAV-hSyn-hChR2(H134R)-EYFP Gift from Karl Deisseroth RRID: Addgene_26973-AAV1 pAAV-CaMKIIa-hChR2(H134R)-EYFP Gift from Karl Deisseroth RRID: Addgene_26969-AAV1 pAAV-hSyn-Cre-P2A-dTomato Gift from Rylan Larsen RRID: Addgene_107738-AAVrg pAAV-hSyn-DIO-hM4D(Gi)-mCherry Gift from Bryan Roth RRID: Addgene_44362-AAV5 Chemicals, peptides, and recombinant proteins Clozapine-n-oxide Enzo Life Sciences Cat#: BML-NS105 DiD stain Thermo Fisher Scientific Cat#: D7757 DiI stain Thermo Fisher Scientific Cat#: D3911 Experimental models: organisms/strains Mouse: Ai14 Jackson Laboratories RRID: IMSR_JAX: 007914 Mouse: C57BL/6J Jackson Laboratories RRID: IMSR_JAX: 000664 Mouse: PV-cre Jackson Laboratories RRID: IMSR_JAX:017320 Software and algorithms Illustrator Adobe RRID: SCR_010279 ImageJ (Fiji) National Institute of Health RRID: SCR_003070 MATLAB MathWorks RRID: SCR_001622 Prism Graphpad RRID: SCR_02798 Other Fiber optic cannula RWD Cat#: R-FOC-L200C-39NA Recording electrode (4 channels) Neuronexus Cat#: Q1x4-10mm-50-177-Q4 ll OPEN ACCESS Current Biology 36, 1–10.e1–e5, April 6, 2026 e1 Report .. Stereotaxic injection of viral vectors Adeno-associated viruses (AAVs) were purchased from Addgene (MA, USA) at titer ≥ 1×1012 vg/ml. pAAV-CaMKIIa-hChR2(H134R)EYFP was a gift from Karl Deisseroth (Addgene plasmid # 26969; http://n2t.net/addgene:26969; RRID:Addgene_26969).78 pAAVCAG-GFP was a gift from Edward Boyden (Addgene viral prep # 37825-AAVrg.T; RRID:Addgene_37825). pAAV-CAG-tdTomato (codon diversified) was a gift from Edward Boyden (viral prep # 59462-AAVrg; RRID:Addgene_59462). pAAV-hSyn-DIO-hM4D(Gi)mCherry was a gift from Bryan Roth (Addgene viral prep # 44362-AAV5); RRID:Addgene_44362).79 pAAV-hSyn-Cre-P2A-dTomato was a gift from Rylan Larsen (Addgene viral prep # 107738-AAVrg; RRID:Addgene_107738).

    Article Title: Targeting of Itch by clomipramine or gene therapy improves cognitive defects related to Alzheimer’s disease
    Article Snippet: .. For preparation of recombinant AAV, mouse WT or T222A/S232A or K393R mutant Itch was subcloned from the constructs described previously in pAAV-CAG-tdTomato vector [(pAAV-CAG-tdTomato (codon diversified)], which was a gift from Edward Boyden (Addgene plasmid #59462; http://addgene.org/59462 ; RRID: Addgene_59462). ..

    Staining:

    Article Title: A developmental switch in corticoclaustral signaling.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken anti-GFP Aves Labs Cat#: gfp1010; RRID: AB_2307313 Rabbit anti-Nr2f2 Abcam Cat#: ab211776; RRID: AB_2893028 Goat anti-Nurr1 R&D Systems Cat#: AF2156; RRID: AB_2153894 Goat anti-PV Swant Cat#: PVG213; RRID: AB_2313848 Rat anti-SST Millipore Cat#: MAB354; RRID: AB_2255365 Mouse anti-Tle4 Santa Cruz Biotechnology Cat#: sc365406; RRID: AB_10841582 Chicken anti-rat IgG Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21472, RRID: AB_2535875 Donkey anti-Chicken IgY Alexa Fluor 488 Thermo Fisher Scientific Cat#: A-78948; RRID: AB_2921070 Donkey anti-Chicken IgY Alexa Fluor 647 Thermo Fisher Scientific Cat#: A-78952; RRID: AB_2921074 Donkey anti-Goat IgG Alexa Fluor 555 Thermo Fisher Scientific Cat# A-21432, RRID: AB_2535853 Donkey anti-Goat IgG Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21447, RRID: AB_2535864 Donkey anti-mouse IgG Alexa Fluor 488 Thermo Fisher Scientific Cat# A-21202, RRID: AB_141607 Bacterial and virus strains pAAV-CAG-GFP Gift from Edward Boyden RRID: Addgene_37825-AAVrg pAAV-CAG-tdTomato Gift from Edward Boyden RRID: Addgene_59462-AAVrg pAAV-hSyn-hChR2(H134R)-EYFP Gift from Karl Deisseroth RRID: Addgene_26973-AAV1 pAAV-CaMKIIa-hChR2(H134R)-EYFP Gift from Karl Deisseroth RRID: Addgene_26969-AAV1 pAAV-hSyn-Cre-P2A-dTomato Gift from Rylan Larsen RRID: Addgene_107738-AAVrg pAAV-hSyn-DIO-hM4D(Gi)-mCherry Gift from Bryan Roth RRID: Addgene_44362-AAV5 Chemicals, peptides, and recombinant proteins Clozapine-n-oxide Enzo Life Sciences Cat#: BML-NS105 DiD stain Thermo Fisher Scientific Cat#: D7757 DiI stain Thermo Fisher Scientific Cat#: D3911 Experimental models: organisms/strains Mouse: Ai14 Jackson Laboratories RRID: IMSR_JAX: 007914 Mouse: C57BL/6J Jackson Laboratories RRID: IMSR_JAX: 000664 Mouse: PV-cre Jackson Laboratories RRID: IMSR_JAX:017320 Software and algorithms Illustrator Adobe RRID: SCR_010279 ImageJ (Fiji) National Institute of Health RRID: SCR_003070 MATLAB MathWorks RRID: SCR_001622 Prism Graphpad RRID: SCR_02798 Other Fiber optic cannula RWD Cat#: R-FOC-L200C-39NA Recording electrode (4 channels) Neuronexus Cat#: Q1x4-10mm-50-177-Q4 ll OPEN ACCESS Current Biology 36, 1–10.e1–e5, April 6, 2026 e1 Report .. Stereotaxic injection of viral vectors Adeno-associated viruses (AAVs) were purchased from Addgene (MA, USA) at titer ≥ 1×1012 vg/ml. pAAV-CaMKIIa-hChR2(H134R)EYFP was a gift from Karl Deisseroth (Addgene plasmid # 26969; http://n2t.net/addgene:26969; RRID:Addgene_26969).78 pAAVCAG-GFP was a gift from Edward Boyden (Addgene viral prep # 37825-AAVrg.T; RRID:Addgene_37825). pAAV-CAG-tdTomato (codon diversified) was a gift from Edward Boyden (viral prep # 59462-AAVrg; RRID:Addgene_59462). pAAV-hSyn-DIO-hM4D(Gi)mCherry was a gift from Bryan Roth (Addgene viral prep # 44362-AAV5); RRID:Addgene_44362).79 pAAV-hSyn-Cre-P2A-dTomato was a gift from Rylan Larsen (Addgene viral prep # 107738-AAVrg; RRID:Addgene_107738).

    Plasmid Preparation:

    Article Title: Hyperinnervation inhibits organ-level regeneration in mammalian skin.
    Article Snippet: All mice were maintained in an AAALAC-accredited (Association for Assessment and Accreditation of Laboratory Animal Care) animal facility at Harvard University. .. pAAV-CAG-tdTomato was a gift from Edward Boyden (Addgene plasmid #59462) and was used as the control AAV plasmid. .. Human wounding experiments were conducted with informed consent under Mass General Brigham Institutional Review Board approval (#2022P000229).

    Article Title: Targeting of Itch by clomipramine or gene therapy improves cognitive defects related to Alzheimer’s disease
    Article Snippet: .. For preparation of recombinant AAV, mouse WT or T222A/S232A or K393R mutant Itch was subcloned from the constructs described previously in pAAV-CAG-tdTomato vector [(pAAV-CAG-tdTomato (codon diversified)], which was a gift from Edward Boyden (Addgene plasmid #59462; http://addgene.org/59462 ; RRID: Addgene_59462). ..

    Control:

    Article Title: Hyperinnervation inhibits organ-level regeneration in mammalian skin.
    Article Snippet: All mice were maintained in an AAALAC-accredited (Association for Assessment and Accreditation of Laboratory Animal Care) animal facility at Harvard University. .. pAAV-CAG-tdTomato was a gift from Edward Boyden (Addgene plasmid #59462) and was used as the control AAV plasmid. .. Human wounding experiments were conducted with informed consent under Mass General Brigham Institutional Review Board approval (#2022P000229).

    Mutagenesis:

    Article Title: Targeting of Itch by clomipramine or gene therapy improves cognitive defects related to Alzheimer’s disease
    Article Snippet: .. For preparation of recombinant AAV, mouse WT or T222A/S232A or K393R mutant Itch was subcloned from the constructs described previously in pAAV-CAG-tdTomato vector [(pAAV-CAG-tdTomato (codon diversified)], which was a gift from Edward Boyden (Addgene plasmid #59462; http://addgene.org/59462 ; RRID: Addgene_59462). ..

    Construct:

    Article Title: Targeting of Itch by clomipramine or gene therapy improves cognitive defects related to Alzheimer’s disease
    Article Snippet: .. For preparation of recombinant AAV, mouse WT or T222A/S232A or K393R mutant Itch was subcloned from the constructs described previously in pAAV-CAG-tdTomato vector [(pAAV-CAG-tdTomato (codon diversified)], which was a gift from Edward Boyden (Addgene plasmid #59462; http://addgene.org/59462 ; RRID: Addgene_59462). ..



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