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pklv u6 sgrna pgkpuro 2a bfp backbone vector  (Addgene inc)


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    Structured Review

    Addgene inc pklv u6 sgrna pgkpuro 2a bfp backbone vector
    Pklv U6 Sgrna Pgkpuro 2a Bfp Backbone Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 171 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/backbone+vector/pKLV-U6gRNA(BbsI)-PGKpuro2ABFP+(Plasmid+%2350946)/pm41841741-385-8-11
    Average 96 stars, based on 171 article reviews
    pklv u6 sgrna pgkpuro 2a bfp backbone vector - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Locus-specific proteomics identifies novel regulators of Epstein-Barr virus lytic reactivation.
    Article Snippet: .. The oligonucleotides were annealed and cloned into the pKLV-U6-sgRNA-PGKPuro-2A-BFP backbone vector (Addgene #50946, kindly deposited by Dr. Kosuke Yusa), which was digested with BbsI. ..

    Article Title: Mbd4 and MutSα protect cells from spontaneous deamination of 5-methylcytosine
    Article Snippet: Cell lines were transduced with FUCas9Cherry (Addgene #70182, a gift from Marco Herold) to drive expression of Cas9-mCherry and enable CRISPR/Cas9 editing [ ]. .. Single guide RNA (sgRNA) sequences targeting mouse Msh6 (TTGGCAAAGGCGCTCCAAAG) and Mbd4 (GATTTTACTGTACTGCCGAA) were cloned into pKLV-U6gRNA (BbsI)-PGKpuro2ABFP (Addgene #50946). ..

    Article Title: DOT1L provides transcriptional memory through PRC1.1 antagonism.
    Article Snippet: .. sgRNA oligonucleotides (IDT) were cloned into lentiviral expression vectors pKLV–U6gRNA(BbsI)–PGKpuro2ABFP (Addgene no. 50946, a gift from Kosuke Yusa). ..

    Plasmid Preparation:

    Article Title: Locus-specific proteomics identifies novel regulators of Epstein-Barr virus lytic reactivation.
    Article Snippet: .. The oligonucleotides were annealed and cloned into the pKLV-U6-sgRNA-PGKPuro-2A-BFP backbone vector (Addgene #50946, kindly deposited by Dr. Kosuke Yusa), which was digested with BbsI. ..

    Article Title: DNA sequence quantitatively encodes CTCF-binding affinity at genome scale
    Article Snippet: .. To assemble the nicking guide RNA (ngRNA) expression vector, the pKLV-U6gRNA (BbsI)-PGKpuro2ABFP plasmid (Addgene; #50946) was digested using BbsI (NEB; R3059L). ..

    Article Title: Cell-type-specific adaptations to mitochondrial stress underly the neurological presentations of MTRFR mutations
    Article Snippet: .. Surviving pools were expanded and knockdown efficiency was assessed by RT-qPCR for MTRFR . pKLV-U6gRNA(BbsI)-PGKpuro2ABFP was a gift from Kosuke Yusa (Addgene plasmid # 50946; http://n2t.net/addgene:50946 ; RRID:Addgene_50946). pMDG and p8.91 were a gift from Simon Davis (Addgene plasmids # 187440, # 187441; http://n2t.net/addgene:187440 , http://n2t.net/addgene:187441 ; RRID:Addgene_187440, RRID:Addgene_187441). ..

    Expressing:

    Article Title: DNA sequence quantitatively encodes CTCF-binding affinity at genome scale
    Article Snippet: .. To assemble the nicking guide RNA (ngRNA) expression vector, the pKLV-U6gRNA (BbsI)-PGKpuro2ABFP plasmid (Addgene; #50946) was digested using BbsI (NEB; R3059L). ..

    Article Title: DOT1L provides transcriptional memory through PRC1.1 antagonism
    Article Snippet: .. Library sgRNAs were expressed in a modified pKLV-U6gRNA(BbsI)- EF1a puro 2A BFP lentiviral sgRNA expression vector that encodes puromycin and BFP selection markers obtained from Addgene no. 50946, a gift from Kosuke Yusa . .. The whole genome Bassik Human CRISPR Knockout Library was obtained from Addgene.

    Article Title: DOT1L provides transcriptional memory through PRC1.1 antagonism.
    Article Snippet: .. sgRNA oligonucleotides (IDT) were cloned into lentiviral expression vectors pKLV–U6gRNA(BbsI)–PGKpuro2ABFP (Addgene no. 50946, a gift from Kosuke Yusa). ..

    Article Title: DOT1L provides transcriptional memory through PRC1.1 antagonism.
    Article Snippet: .. Library sgRNAs were expressed in a modified pKLV-U6gRNA(BbsI)-EF1apuro2ABFP lentiviral sgRNA expression vector that encodes puromycin and BFP selection markers obtained from Addgene no. 50946, a gift from Kosuke Yusa61. .. The whole genome Bassik Human CRISPR Knockout Library was obtained from Addgene.

    Modification:

    Article Title: DOT1L provides transcriptional memory through PRC1.1 antagonism
    Article Snippet: .. Library sgRNAs were expressed in a modified pKLV-U6gRNA(BbsI)- EF1a puro 2A BFP lentiviral sgRNA expression vector that encodes puromycin and BFP selection markers obtained from Addgene no. 50946, a gift from Kosuke Yusa . .. The whole genome Bassik Human CRISPR Knockout Library was obtained from Addgene.

    Article Title: DOT1L provides transcriptional memory through PRC1.1 antagonism.
    Article Snippet: .. Library sgRNAs were expressed in a modified pKLV-U6gRNA(BbsI)-EF1apuro2ABFP lentiviral sgRNA expression vector that encodes puromycin and BFP selection markers obtained from Addgene no. 50946, a gift from Kosuke Yusa61. .. The whole genome Bassik Human CRISPR Knockout Library was obtained from Addgene.

    Selection:

    Article Title: DOT1L provides transcriptional memory through PRC1.1 antagonism
    Article Snippet: .. Library sgRNAs were expressed in a modified pKLV-U6gRNA(BbsI)- EF1a puro 2A BFP lentiviral sgRNA expression vector that encodes puromycin and BFP selection markers obtained from Addgene no. 50946, a gift from Kosuke Yusa . .. The whole genome Bassik Human CRISPR Knockout Library was obtained from Addgene.

    Article Title: DOT1L provides transcriptional memory through PRC1.1 antagonism.
    Article Snippet: .. Library sgRNAs were expressed in a modified pKLV-U6gRNA(BbsI)-EF1apuro2ABFP lentiviral sgRNA expression vector that encodes puromycin and BFP selection markers obtained from Addgene no. 50946, a gift from Kosuke Yusa61. .. The whole genome Bassik Human CRISPR Knockout Library was obtained from Addgene.

    Knockdown:

    Article Title: Cell-type-specific adaptations to mitochondrial stress underly the neurological presentations of MTRFR mutations
    Article Snippet: .. Surviving pools were expanded and knockdown efficiency was assessed by RT-qPCR for MTRFR . pKLV-U6gRNA(BbsI)-PGKpuro2ABFP was a gift from Kosuke Yusa (Addgene plasmid # 50946; http://n2t.net/addgene:50946 ; RRID:Addgene_50946). pMDG and p8.91 were a gift from Simon Davis (Addgene plasmids # 187440, # 187441; http://n2t.net/addgene:187440 , http://n2t.net/addgene:187441 ; RRID:Addgene_187440, RRID:Addgene_187441). ..

    Quantitative RT-PCR:

    Article Title: Cell-type-specific adaptations to mitochondrial stress underly the neurological presentations of MTRFR mutations
    Article Snippet: .. Surviving pools were expanded and knockdown efficiency was assessed by RT-qPCR for MTRFR . pKLV-U6gRNA(BbsI)-PGKpuro2ABFP was a gift from Kosuke Yusa (Addgene plasmid # 50946; http://n2t.net/addgene:50946 ; RRID:Addgene_50946). pMDG and p8.91 were a gift from Simon Davis (Addgene plasmids # 187440, # 187441; http://n2t.net/addgene:187440 , http://n2t.net/addgene:187441 ; RRID:Addgene_187440, RRID:Addgene_187441). ..



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