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BlueGnome Limited bac microarray
Cytogenetic analysis of a cleavage stage embryo from a Robertsonian translocation carrier- 46,XY,t(13;15)(q21.3;q11.2). <t>Microarray-CGH</t> revealed monosomy 13, presumably resulting from a meiotic error due to problems processing the rearranged chromosomes. An additional aneuploidy unrelated to the Robertsonian translocation (monosomy for <t>chromosome</t> 1) was also detected. The two monosomies are indicated by the altered ratio of fluorescence related to the test (embryo) and reference (46,XY) DNA samples. All of the probes corresponding to chromosomes 1 and 13 have test/reference ratios less than 0.3.
Bac Microarray, supplied by BlueGnome Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bac+microarray+cy3/24sure+v3+arrays/pmc03486902-351-21-26
Average 90 stars, based on 1 article reviews
bac microarray - by Bioz Stars, 2026-10
90/100 stars

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1) Product Images from "Embryos of Robertsonian Translocation Carriers Exhibit a Mitotic Interchromosomal Effect That Enhances Genetic Instability during Early Development"

Article Title: Embryos of Robertsonian Translocation Carriers Exhibit a Mitotic Interchromosomal Effect That Enhances Genetic Instability during Early Development

Journal: PLoS Genetics

doi: 10.1371/journal.pgen.1003025

Cytogenetic analysis of a cleavage stage embryo from a Robertsonian translocation carrier- 46,XY,t(13;15)(q21.3;q11.2). Microarray-CGH revealed monosomy 13, presumably resulting from a meiotic error due to problems processing the rearranged chromosomes. An additional aneuploidy unrelated to the Robertsonian translocation (monosomy for chromosome 1) was also detected. The two monosomies are indicated by the altered ratio of fluorescence related to the test (embryo) and reference (46,XY) DNA samples. All of the probes corresponding to chromosomes 1 and 13 have test/reference ratios less than 0.3.
Figure Legend Snippet: Cytogenetic analysis of a cleavage stage embryo from a Robertsonian translocation carrier- 46,XY,t(13;15)(q21.3;q11.2). Microarray-CGH revealed monosomy 13, presumably resulting from a meiotic error due to problems processing the rearranged chromosomes. An additional aneuploidy unrelated to the Robertsonian translocation (monosomy for chromosome 1) was also detected. The two monosomies are indicated by the altered ratio of fluorescence related to the test (embryo) and reference (46,XY) DNA samples. All of the probes corresponding to chromosomes 1 and 13 have test/reference ratios less than 0.3.

Techniques Used: Translocation Assay, Microarray, Fluorescence

Related Articles

other:

Article Title: Assessment of risk of aneuploidy
Article Snippet: All of the above errors in meiosis I can be detected by quantitation using prior art methods such as 24SureTM (BlueGnome, Cambridge UK) following manufacturer's protocol.

Article Title: Prediction of live birth - selection of embryos using morphokinetic parameters.
Article Snippet: The obtained DNA sample with a final concentration of about 50 ng/μL was examined by arrayCGH (DNA labeling, on-chip hybridization – 24sure +, BlueGnome, Illumina, analysis using BlueFuse Multi software) or the chromosomal profile of the samples was determined by NGS (VeriSeq PGS Kit, Illumina).

Article Title: The Ratio of Serum Progesterone (P4) to the Number of Follicles (P4/follicle) is a More Objective Parameter for Euploidy Rate as Compared to Systemic Progesterone Levels.
Article Snippet: Does the late follicular phase progesterone (P4) and the P4-to-follicle-ratio affect the ploidy state of the biopsied embryos?. A retrospective observational study conducted at ART Fertility Clinics Abu Dhabi and Muscat, including all stimulation cycles performed between January 2015 and December 2019.. In total, 975 cycles were considered for this study.

Article Title: Non-invasive preimplantation genetic testing for putative mosaic blastocysts: a pilot study.
Article Snippet: testing for putative mosaic blastocysts: a pilot study Xinyuan Li , Yan Hao, Dawei Chen, Dongmei Ji, Wanbo Zhu, Xiaoqian Zhu, Zhaolian Wei, Yunxia Cao, Zhiguo Zhang *, and Ping Zhou * Department of Obstetrics and Gynecology, Reproductive Medicine Center, the First Affiliated Hospital of Anhui Medical University, Anhui, China NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract, Anhui Medical University, Hefei, Anhui, China Key Laboratory of Population Health Across Life Cycle, Anhui Medical University, Ministry of Education of the People’s Republic of China, Hefei, Anhui, China Anhui Province Key Laboratory of Reproductive Health and Genetics, Hefei, Anhui, China Biopreservation and Artificial Organs, Anhui Provincial Engineering Research Center, Anhui Medical University, Hefei, Anhui, China Affiliated Anhui Provincial Hospital of Anhui Medical University, Anhui, China

Whole Genome Amplification:

Article Title: Can sperm quality influence embryo development and its ploidy? Analysis of 811 blastocysts obtained from different sperm sources
Article Snippet: The aim of our study was to evaluate the correlation between sperm quality and ploidy status of the derived blastocysts.. We performed a retrospective analysis on a restricted pool of patients enrolling only those who had no female factors.. Male patients with genetic factors affecting spermatogenesis were also excluded.

Control:

Article Title: Can sperm quality influence embryo development and its ploidy? Analysis of 811 blastocysts obtained from different sperm sources
Article Snippet: The aim of our study was to evaluate the correlation between sperm quality and ploidy status of the derived blastocysts.. We performed a retrospective analysis on a restricted pool of patients enrolling only those who had no female factors.. Male patients with genetic factors affecting spermatogenesis were also excluded.

Amplification:

Article Title: Blastocyst score, a blastocyst quality ranking tool, is a predictor of blastocyst ploidy and implantation potential
Article Snippet: The TE biopsy was performed after BL was graded on D5 (108–115 hours after insemination) or D6 (130–138 hours after insemination), and up to 10 cells were collected after zona pellucida dissection and hatching by laser (ZILOS-tk, Hamilton Thorne). .. Biopsied samples were amplified and analyzed by either array comparative genomic hybridization (BlueGnome 24sure; Illumina, single nucleotide polymorphism (Natera), or next-generation sequencing (NGS-low, Illumina). ..

Hybridization:

Article Title: Blastocyst score, a blastocyst quality ranking tool, is a predictor of blastocyst ploidy and implantation potential
Article Snippet: The TE biopsy was performed after BL was graded on D5 (108–115 hours after insemination) or D6 (130–138 hours after insemination), and up to 10 cells were collected after zona pellucida dissection and hatching by laser (ZILOS-tk, Hamilton Thorne). .. Biopsied samples were amplified and analyzed by either array comparative genomic hybridization (BlueGnome 24sure; Illumina, single nucleotide polymorphism (Natera), or next-generation sequencing (NGS-low, Illumina). ..

Next-Generation Sequencing:

Article Title: Blastocyst score, a blastocyst quality ranking tool, is a predictor of blastocyst ploidy and implantation potential
Article Snippet: The TE biopsy was performed after BL was graded on D5 (108–115 hours after insemination) or D6 (130–138 hours after insemination), and up to 10 cells were collected after zona pellucida dissection and hatching by laser (ZILOS-tk, Hamilton Thorne). .. Biopsied samples were amplified and analyzed by either array comparative genomic hybridization (BlueGnome 24sure; Illumina, single nucleotide polymorphism (Natera), or next-generation sequencing (NGS-low, Illumina). ..



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Innopsys Inc bac microarray cy3
<t>Cy3</t> and Cy5 fluorescence values obtained from Human Core Exome SNP array (Illumina) for 200 ng diploid gDNA (left), flow-sorted-then-amplified single chromosome 6 sample #1 (centre), and flow-sorted-then-amplified single chromosome 6 sample #2 (right).
Bac Microarray Cy3, supplied by Innopsys Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bac+microarray+cy3/bac+microarray+cy3/pmc04961964-130-14-19
Average 90 stars, based on 1 article reviews
bac microarray cy3 - by Bioz Stars, 2026-10
90/100 stars
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Image Search Results


Cy3 and Cy5 fluorescence values obtained from Human Core Exome SNP array (Illumina) for 200 ng diploid gDNA (left), flow-sorted-then-amplified single chromosome 6 sample #1 (centre), and flow-sorted-then-amplified single chromosome 6 sample #2 (right).

Journal: Scientific Reports

Article Title: Haplotyping the human leukocyte antigen system from single chromosomes

doi: 10.1038/srep30381

Figure Lengend Snippet: Cy3 and Cy5 fluorescence values obtained from Human Core Exome SNP array (Illumina) for 200 ng diploid gDNA (left), flow-sorted-then-amplified single chromosome 6 sample #1 (centre), and flow-sorted-then-amplified single chromosome 6 sample #2 (right).

Article Snippet: Briefly, DNA samples were labelled with random primers, hybridised to a BAC microarray with Cy3, scanned on InnoScan 710 (Innopsys) and reference compared against a euploid male labelled with Cy5.

Techniques: Fluorescence, Amplification