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anti mhci  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank anti mhci
    Anti Mhci, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axis/anti-Myosin+heavy+chain/pmc12828604-85-48-53
    Average 96 stars, based on 19 article reviews
    anti mhci - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: EMILIN1 emerges as a TGFβ/SETDB1-regulated secreted biomarker in Duchenne muscular dystrophy.
    Article Snippet: Widefield fluorescence images were taken with an Axio Observer 7 AR TI CL E IN P RE SS microscope (Zeiss) equipped with a motorized stage coupled to an Orca Flash 4 Camera (Hamamatsu) and driven by the Zen software (Zeiss). .. Antibodies Antibody Reference Use and dilution Myosin heavy chain, MYH1E (mouse IgG2b) DSHB MF20 Immunofluorescence (1:10) PAI1 (rabbit) Proteintech, 13801-1-AP Western blot (1:1000) MyoD (rabbit) Cell Signaling Technology, 13812 Western blot (1:1000) Vinculin (mouse IgG1) Sigma-Aldrich, V9131 Western blot (1:500) Emilin1 (rabbit) Kind gift from Paola Spessotto, As-556 Immunofluorescence (1:1000) Emilin1 (rabbit) Abcam, AB185953 Immunofluorescence (1:100) Laminin (rabbit) Dako, Z0097 Immunofluorescence (1:400) .. Total RNA from cells was extracted using RNeasy Micro Kit (Qiagen, 74004) following the manufacturer’s procedures.

    Western Blot:

    Article Title: EMILIN1 emerges as a TGFβ/SETDB1-regulated secreted biomarker in Duchenne muscular dystrophy.
    Article Snippet: Widefield fluorescence images were taken with an Axio Observer 7 AR TI CL E IN P RE SS microscope (Zeiss) equipped with a motorized stage coupled to an Orca Flash 4 Camera (Hamamatsu) and driven by the Zen software (Zeiss). .. Antibodies Antibody Reference Use and dilution Myosin heavy chain, MYH1E (mouse IgG2b) DSHB MF20 Immunofluorescence (1:10) PAI1 (rabbit) Proteintech, 13801-1-AP Western blot (1:1000) MyoD (rabbit) Cell Signaling Technology, 13812 Western blot (1:1000) Vinculin (mouse IgG1) Sigma-Aldrich, V9131 Western blot (1:500) Emilin1 (rabbit) Kind gift from Paola Spessotto, As-556 Immunofluorescence (1:1000) Emilin1 (rabbit) Abcam, AB185953 Immunofluorescence (1:100) Laminin (rabbit) Dako, Z0097 Immunofluorescence (1:400) .. Total RNA from cells was extracted using RNeasy Micro Kit (Qiagen, 74004) following the manufacturer’s procedures.

    Article Title: PGC-1α pathway dysregulation disrupts myofiber specification in a mouse model of SBMA.
    Article Snippet: .. The following primary antibodies (antigen, dilution/concentration, vendor, catalog number) were used for these studies: Myh4, 1:100, Developmental Studies Hybridoma Bank at the University of Iowa (DSHB), BF-F3; Myh1, 1:10, DSHB, 6H1; PGC-1α (ChIP-seq), Novus, NBP1-04676; PGC-1α (Western blot), 1:1000, Abcam, ab191838; Vinculin, 1:10,000, Sigma Aldrich, V9131; MEF2A/C, 1:100, Abcam, ab197070; CBP, 1:200, Invitrogen, PA5-27369; CREB, 1:500, Cell Signaling Technology, D76D11; p62, 1:400, PROGEN, GP62-C; PG-21 (AR), 1:500, Millipore, 06- 680. ..

    Article Title: Diaphragm hypertrophy and spinal stress response accompany phrenic myelin defects in a murine model of Charcot-Marie-Tooth Disease Type 1E.
    Article Snippet: Charcot-Marie-Tooth disease type 1E (CMT1E) is a rare, early-onset dysmyelinating neuropathy linked with point mutations in the peripheral myelin protein 22 (PMP22) gene.. Respiratory problems are known to impact neuropathic patients, yet existing studies have not characterized this pathophysiology in corresponding animal models.. Trembler J (TrJ) mice carry the same Leu16Pro amino acid substitution in the PMP22 protein that is present in families diagnosed with CMT1E.

    Pyrolysis Gas Chromatography:

    Article Title: PGC-1α pathway dysregulation disrupts myofiber specification in a mouse model of SBMA.
    Article Snippet: .. The following primary antibodies (antigen, dilution/concentration, vendor, catalog number) were used for these studies: Myh4, 1:100, Developmental Studies Hybridoma Bank at the University of Iowa (DSHB), BF-F3; Myh1, 1:10, DSHB, 6H1; PGC-1α (ChIP-seq), Novus, NBP1-04676; PGC-1α (Western blot), 1:1000, Abcam, ab191838; Vinculin, 1:10,000, Sigma Aldrich, V9131; MEF2A/C, 1:100, Abcam, ab197070; CBP, 1:200, Invitrogen, PA5-27369; CREB, 1:500, Cell Signaling Technology, D76D11; p62, 1:400, PROGEN, GP62-C; PG-21 (AR), 1:500, Millipore, 06- 680. ..

    Chromatin Immunoprecipitation:

    Article Title: PGC-1α pathway dysregulation disrupts myofiber specification in a mouse model of SBMA.
    Article Snippet: .. The following primary antibodies (antigen, dilution/concentration, vendor, catalog number) were used for these studies: Myh4, 1:100, Developmental Studies Hybridoma Bank at the University of Iowa (DSHB), BF-F3; Myh1, 1:10, DSHB, 6H1; PGC-1α (ChIP-seq), Novus, NBP1-04676; PGC-1α (Western blot), 1:1000, Abcam, ab191838; Vinculin, 1:10,000, Sigma Aldrich, V9131; MEF2A/C, 1:100, Abcam, ab197070; CBP, 1:200, Invitrogen, PA5-27369; CREB, 1:500, Cell Signaling Technology, D76D11; p62, 1:400, PROGEN, GP62-C; PG-21 (AR), 1:500, Millipore, 06- 680. ..

    other:

    Article Title:
    Article Snippet: Mouse monoclonal anti-eMyHC , DSHB , Cat# F1.652, RRID: AB_528358.

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals
    Article Snippet: The fiber type of individual single fiber segments was determined using the dot blotting method previously described and now termed MyDoBID (Myosin heavy chain detection by Dot Blotting for IDentification of muscle fiber type)., Briefly, a polyvinylidene fluoride (PVDF) membrane was activated in 96% ethanol for 120 s and equilibrated in transfer buffer (Bio-Rad Laboratories Laboratories, Hercules, CA, USA) for 120 s. Following this, 1-μL (10%) of each fiber was spotted on activated PVDF membrane.

    Immunostaining:

    Article Title: Diaphragm hypertrophy and spinal stress response accompany phrenic myelin defects in a murine model of Charcot-Marie-Tooth Disease Type 1E.
    Article Snippet: Charcot-Marie-Tooth disease type 1E (CMT1E) is a rare, early-onset dysmyelinating neuropathy linked with point mutations in the peripheral myelin protein 22 (PMP22) gene.. Respiratory problems are known to impact neuropathic patients, yet existing studies have not characterized this pathophysiology in corresponding animal models.. Trembler J (TrJ) mice carry the same Leu16Pro amino acid substitution in the PMP22 protein that is present in families diagnosed with CMT1E.

    Blocking Assay:

    Article Title: Gut microbiota-derived taurolithocholic acid modulates myofiber-type switching via p38 MAPK/PGC-1α signaling underlying breed differences between Arbor Acres and Taoyuan chickens
    Article Snippet: .. Following blocking with 1% BSA, the sections were incubated with the primary antibody MYH1A (1:100, F59, DSHB, USA) and the AF647-labeled goat anti-mouse secondary antibody (1:500, A0473, Beyotime), with the primary antibody MYH7B (1:100, S58, DSHB) and the FITC-labeled goat anti-mouse secondary antibody (1:500, A0568, Beyotime). .. Cell nuclei were stained with DAPI (C1002, Beyotime).

    Incubation:

    Article Title: Gut microbiota-derived taurolithocholic acid modulates myofiber-type switching via p38 MAPK/PGC-1α signaling underlying breed differences between Arbor Acres and Taoyuan chickens
    Article Snippet: .. Following blocking with 1% BSA, the sections were incubated with the primary antibody MYH1A (1:100, F59, DSHB, USA) and the AF647-labeled goat anti-mouse secondary antibody (1:500, A0473, Beyotime), with the primary antibody MYH7B (1:100, S58, DSHB) and the FITC-labeled goat anti-mouse secondary antibody (1:500, A0568, Beyotime). .. Cell nuclei were stained with DAPI (C1002, Beyotime).



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