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fluorescence microscope axioobserver 20× objective, axiocam 503 mono, filter cube for red dyes  (Carl Zeiss)


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    Structured Review

    Carl Zeiss fluorescence microscope axioobserver 20× objective, axiocam 503 mono, filter cube for red dyes
    Fluorescence Microscope Axioobserver 20× Objective, Axiocam 503 Mono, Filter Cube For Red Dyes, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioobserver+fluorescence+microscope/axiocam+camera+mrm/pmc12270789-96-11-14
    Average 90 stars, based on 1 article reviews
    fluorescence microscope axioobserver 20× objective, axiocam 503 mono, filter cube for red dyes - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Inverted Microscopy:

    Article Title: A gene regulatory element modulates myosin expression and controls cardiomyocyte response to stress
    Article Snippet: .. Cells were imaged using a Zeiss AxioObserver7 inverted microscope with a Zeiss Axiocam 305 color camera. .. The following primary antibody was used: Rabbit anti-TNNT2 (abcam, 1:500).

    Microscopy:

    Article Title: CD44 Participates to Extramedullary Haematopoiesis Onset by Mediating the Interplay Between Monocytes and Haematopoietic Stem Cells in Myelofibrosis
    Article Snippet: Organ sections were stained according to the haematoxylin and eosin protocol (Bio‐Optica, Milan, Italy) or subjected to immunohistochemical analysis using antibodies against murine F4/80 (ThermoFisher cat. #14‐4801‐82, Waltham, Massachussets, USA) by means of the Ventana BenchMark Discovery XT (Roche, Swiss). .. The images were captured by using an Axioscope A1 microscope equipped with an AxioCam ERc 5S Digital Camera and Axion software 4.8 (all Carl Zeiss MicroImaging Inc.; Thornwood, NY, USA). .. Megakaryocyte count was performed with ImageJ (Rasband, W.S., National Institutes of Health, USA), with the plugin Cell_Counter.

    Article Title: Semaphorin 3f and post-embryonic regulation of retinal progenitors.
    Article Snippet: Images of WM labelling were collected on a Stemi SV 11 stereoscope using an AxioCam HRc camera with ZEN (Zeiss) software. .. Retinal sections were imaged on a Zeiss Axioplan 2 microscope with an AxioCam MRc camera using ZEN (Zeiss) software. .. Images of FISH in WM and on retinal sections were taken using an LSM 900 confocal microscope with an AxioCam 305 camera using ZenBlue (Zeiss) software.

    Article Title: Protocol for monitoring of neutralizing antibodies to various receptor-binding domains using the tANCHOR system
    Article Snippet: .. Fluorescence microscope AxioObserver 20× objective, Axiocam 503 mono, filter cube for red dyes , Zeiss , www.zeiss.com. .. Confocal laser scanning microscope LSM 780 , Zeiss , www.zeiss.com.

    Software:

    Article Title: CD44 Participates to Extramedullary Haematopoiesis Onset by Mediating the Interplay Between Monocytes and Haematopoietic Stem Cells in Myelofibrosis
    Article Snippet: Organ sections were stained according to the haematoxylin and eosin protocol (Bio‐Optica, Milan, Italy) or subjected to immunohistochemical analysis using antibodies against murine F4/80 (ThermoFisher cat. #14‐4801‐82, Waltham, Massachussets, USA) by means of the Ventana BenchMark Discovery XT (Roche, Swiss). .. The images were captured by using an Axioscope A1 microscope equipped with an AxioCam ERc 5S Digital Camera and Axion software 4.8 (all Carl Zeiss MicroImaging Inc.; Thornwood, NY, USA). .. Megakaryocyte count was performed with ImageJ (Rasband, W.S., National Institutes of Health, USA), with the plugin Cell_Counter.

    Light Microscopy:

    Article Title: A novel oleogel-in-oleogel system with tailorable digestibility through kinetic confinement of the oil
    Article Snippet: .. The samples were visualized using a Zeiss Axio Lab A1 optical bright field and polarized light microscope (Oberkochen, Germany) connected to a Zeiss Axiocam 305 colour camera. ..

    Imaging:

    Article Title: Protocol for induction and study of DNA double-strand breaks in mammalian cells using PALM microdissection and expansion microscopy
    Article Snippet: .. For imaging using Axiovision 4.9 (Zeiss AG), objectives LD Plan-neofluar 40×/0,6 Ph2 Korr M27 and Plan-neofluar 63×/0,75 Korr ∞/0–1.5, as well as a camera Axiocam MRc5 were used ( A and : A 48-h time-lapse of microirradiated HeLa FUCCI cells to assess viability, related to setup). ..

    Fluorescence:

    Article Title: Protocol for monitoring of neutralizing antibodies to various receptor-binding domains using the tANCHOR system
    Article Snippet: .. Fluorescence microscope AxioObserver 20× objective, Axiocam 503 mono, filter cube for red dyes , Zeiss , www.zeiss.com. .. Confocal laser scanning microscope LSM 780 , Zeiss , www.zeiss.com.

    other:

    Article Title: Assessment of the plasticity of dry granulated particles (mini-tablets) and its relationship to their tabletability
    Article Snippet: An image of the tablet surface was captured using a light microscope (Zeiss AxioCam ICc 5, Carl Zeiss AB, Sweden), and the diameter of the circular dent ( d ) was measured using the length measurement tool available in the microscope software (AxioVision, Carl Zeiss AB, Sweden).



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    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging <t>Microscope</t> with transillumination ( Trans ) or <t>fluorescence</t> illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.
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    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging <t>Microscope</t> with transillumination ( Trans ) or <t>fluorescence</t> illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.
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    Carl Zeiss axioobserver a1 fluorescence microscope
    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging <t>Microscope</t> with transillumination ( Trans ) or <t>fluorescence</t> illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.
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    Image Search Results


    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging Microscope with transillumination ( Trans ) or fluorescence illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.

    Journal: bioRxiv

    Article Title: Membrane-anchored PrP Sc is the trigger for prion synaptotoxicity

    doi: 10.1101/2025.07.11.664221

    Figure Lengend Snippet: (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging Microscope with transillumination ( Trans ) or fluorescence illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.

    Article Snippet: 10-15 neurons were randomly selected and imaged using a Zeiss AxioObserver D1 Fluorescence Microscope and/or a Zeiss LSM 700 Laser Scanning Confocal Microscope with 63X oil objectives.

    Techniques: Control, Expressing, Live Cell Imaging, Microscopy, Fluorescence, Transduction, Staining, Comparison