Review



axioobserver z1 inverted fluorescence microscope  (Carl Zeiss)


Bioz Verified Symbol Carl Zeiss is a verified supplier
Bioz Manufacturer Symbol Carl Zeiss manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Carl Zeiss axioobserver z1 inverted fluorescence microscope
    Axioobserver Z1 Inverted Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 4893 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioobserver+fluorescence+microscope/Inverted+microscope+Axio+Observer+3/pmc12167074-414-7-6
    Average 99 stars, based on 4893 article reviews
    axioobserver z1 inverted fluorescence microscope - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Interaction between FmRAV4 (a RAV transcription factor) and FmMYB1 (a MYB transcription factor) confers drought tolerance via ROS scavenging in Fraxinus mandshurica.
    Article Snippet: Drought stress severely restricts plant growth and development.. Although AP2/ERF family transcription factors (TFs) participate extensively in abiotic stress responses, they remain largely unexplored in Fraxinus mandshurica.. In this study, we identified and functionally characterized FmRAV4, a TF from the RAV subfamily.

    Staining:

    Article Title: Interaction between FmRAV4 (a RAV transcription factor) and FmMYB1 (a MYB transcription factor) confers drought tolerance via ROS scavenging in Fraxinus mandshurica.
    Article Snippet: Drought stress severely restricts plant growth and development.. Although AP2/ERF family transcription factors (TFs) participate extensively in abiotic stress responses, they remain largely unexplored in Fraxinus mandshurica.. In this study, we identified and functionally characterized FmRAV4, a TF from the RAV subfamily.

    Article Title: Photoinduced hydrogel coatings hybridized with silver nanoparticles to prevent Nitratidesulfovibrio vulgaris adhesion and bio-corrosion of aluminum substrates
    Article Snippet: At the start of the experiment, the optical density at 600 nm was 0.014, measured using a Spectronic 401 (Spectronic Instruments, Milton Roy, USA). .. Bacteria cells were stained with SYTO9 (1 μL, Invitrogen, S34854) for initial counting via epifluorescence microscopy with an Axio Observer Z1 inverted fluorescence microscope (Zeiss, Jean, Germany), yielding a concentration of 1.33×107 cells/mL. ..

    Article Title: Programmable Relaxation Dynamics in Bioinspired Coacervates for 3D Printing Cell Scaffolds.
    Article Snippet: Inspired by the tunable physical properties and biological roles of intracellular biomolecular condensates, this study developed chitosan and hyaluronic acid coacervates via liquid–liquid phase separation for 3D-printed cell scaffolds.. Multiscale analysis revealed that relaxation dynamics governed the structural organization and extrusion processability of coacervates.. Using timesalt-molecular weight-pH superposition principles, relaxation times were continuously and predictably tuned across seven orders of magnitude (10− 3 to 104 s) by modulating pH, ionic strength, and chain length, enabling programmable solid-gel-liquid transitions through a dynamic balance of electrostatic, hydrophobic, and hydration interactions.

    Fluorescence:

    Article Title: Interaction between FmRAV4 (a RAV transcription factor) and FmMYB1 (a MYB transcription factor) confers drought tolerance via ROS scavenging in Fraxinus mandshurica.
    Article Snippet: Drought stress severely restricts plant growth and development.. Although AP2/ERF family transcription factors (TFs) participate extensively in abiotic stress responses, they remain largely unexplored in Fraxinus mandshurica.. In this study, we identified and functionally characterized FmRAV4, a TF from the RAV subfamily.

    Article Title: Biomimetic mineralized hydrogels loaded with strontium-proanthocyanidins coordination particles for bone defect repair.
    Article Snippet: .. Fluorescence images were acquired using an inverted fluorescence microscope (Zeiss Axio Vert. ..

    Article Title: Modulating fibrillogenesis enhances the biofunctionality of dense collagen hydrogels
    Article Snippet: Slides were mounted using Vectamount AQ Aqueous Mounting Medium (Vector Laboratories) and cured overnight at room temperature. .. Sections were imaged using a Zeiss Axio-Observer.Z1 inverted fluorescence microscope for immunofluorescent detection or bright-field microscopy for H&E and chromogenic DAB stains. ..

    Article Title: Photoinduced hydrogel coatings hybridized with silver nanoparticles to prevent Nitratidesulfovibrio vulgaris adhesion and bio-corrosion of aluminum substrates
    Article Snippet: At the start of the experiment, the optical density at 600 nm was 0.014, measured using a Spectronic 401 (Spectronic Instruments, Milton Roy, USA). .. Bacteria cells were stained with SYTO9 (1 μL, Invitrogen, S34854) for initial counting via epifluorescence microscopy with an Axio Observer Z1 inverted fluorescence microscope (Zeiss, Jean, Germany), yielding a concentration of 1.33×107 cells/mL. ..

    Article Title: Programmable Relaxation Dynamics in Bioinspired Coacervates for 3D Printing Cell Scaffolds.
    Article Snippet: Inspired by the tunable physical properties and biological roles of intracellular biomolecular condensates, this study developed chitosan and hyaluronic acid coacervates via liquid–liquid phase separation for 3D-printed cell scaffolds.. Multiscale analysis revealed that relaxation dynamics governed the structural organization and extrusion processability of coacervates.. Using timesalt-molecular weight-pH superposition principles, relaxation times were continuously and predictably tuned across seven orders of magnitude (10− 3 to 104 s) by modulating pH, ionic strength, and chain length, enabling programmable solid-gel-liquid transitions through a dynamic balance of electrostatic, hydrophobic, and hydration interactions.

    Article Title: IscR-mediated morphological regulation confers virulence and stress resistance by reducing stress molecule uptake in Acinetobacter baumannii .
    Article Snippet: .. Fixed bacteria were immobilized on agarose pads and imaged using a Zeiss LSM 880 inverted confocal microscope or a Zeiss AxioObserver.Z1 inverted fluorescence microscope with a 63× oil immersion objective and a laser line of 488 nm for detection. ..

    Microscopy:

    Article Title: Interaction between FmRAV4 (a RAV transcription factor) and FmMYB1 (a MYB transcription factor) confers drought tolerance via ROS scavenging in Fraxinus mandshurica.
    Article Snippet: Drought stress severely restricts plant growth and development.. Although AP2/ERF family transcription factors (TFs) participate extensively in abiotic stress responses, they remain largely unexplored in Fraxinus mandshurica.. In this study, we identified and functionally characterized FmRAV4, a TF from the RAV subfamily.

    Article Title: Biomimetic mineralized hydrogels loaded with strontium-proanthocyanidins coordination particles for bone defect repair.
    Article Snippet: .. Fluorescence images were acquired using an inverted fluorescence microscope (Zeiss Axio Vert. ..

    Article Title: Computational identification and experimental validation of early glycinergic impairments in spinal muscular atrophy
    Article Snippet: Probes were purchased from ACD: Article in Press Scientific Reports https://doi.org/10.1038/s41598-026-74597-y Mm-Gcsh-O1-C1 (catalog #1240481-C1) and Mm-ChAT-C2 (catalog #408731-C2). .. The hybridized probe signal was acquired using a Zeiss Axio-observer Z1 inverted confocal microscope with a 25× oil objective. .. Spinal cord samples were homogenized in radioimmunoprecipitation assay buffer (RIPA lysis buffer, Merck Life Sciences, Darmstadt, Germany, ref. 20–188) supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF, Thermo Fisher Scientific, ref. 36978), 1 mM dithiothreitol (DTT, Thermo Fisher Scientific, ref. R0861), 2 mM sodium orthovanadate (Merck Life Sciences, ref. S6508) and 1 × complete Protease Inhibitor Cocktail (Merck Life Sciences, ref. 11697498001).

    Article Title: Modulating fibrillogenesis enhances the biofunctionality of dense collagen hydrogels
    Article Snippet: Slides were mounted using Vectamount AQ Aqueous Mounting Medium (Vector Laboratories) and cured overnight at room temperature. .. Sections were imaged using a Zeiss Axio-Observer.Z1 inverted fluorescence microscope for immunofluorescent detection or bright-field microscopy for H&E and chromogenic DAB stains. ..

    Article Title: Photoinduced hydrogel coatings hybridized with silver nanoparticles to prevent Nitratidesulfovibrio vulgaris adhesion and bio-corrosion of aluminum substrates
    Article Snippet: At the start of the experiment, the optical density at 600 nm was 0.014, measured using a Spectronic 401 (Spectronic Instruments, Milton Roy, USA). .. Bacteria cells were stained with SYTO9 (1 μL, Invitrogen, S34854) for initial counting via epifluorescence microscopy with an Axio Observer Z1 inverted fluorescence microscope (Zeiss, Jean, Germany), yielding a concentration of 1.33×107 cells/mL. ..

    Article Title: Programmable Relaxation Dynamics in Bioinspired Coacervates for 3D Printing Cell Scaffolds.
    Article Snippet: Inspired by the tunable physical properties and biological roles of intracellular biomolecular condensates, this study developed chitosan and hyaluronic acid coacervates via liquid–liquid phase separation for 3D-printed cell scaffolds.. Multiscale analysis revealed that relaxation dynamics governed the structural organization and extrusion processability of coacervates.. Using timesalt-molecular weight-pH superposition principles, relaxation times were continuously and predictably tuned across seven orders of magnitude (10− 3 to 104 s) by modulating pH, ionic strength, and chain length, enabling programmable solid-gel-liquid transitions through a dynamic balance of electrostatic, hydrophobic, and hydration interactions.

    Article Title: IscR-mediated morphological regulation confers virulence and stress resistance by reducing stress molecule uptake in Acinetobacter baumannii .
    Article Snippet: .. Fixed bacteria were immobilized on agarose pads and imaged using a Zeiss LSM 880 inverted confocal microscope or a Zeiss AxioObserver.Z1 inverted fluorescence microscope with a 63× oil immersion objective and a laser line of 488 nm for detection. ..

    Article Title: Early myelin-associated histological and cellular findings following ketogenic diet or ketone ester treatment in a cuprizone/rapamycin mouse model.
    Article Snippet: Background: Impaired restoration of CNS myelin contributes to progressive disability in multiple sclerosis (MS), yet current disease-modifying therapies do not directly target this biology.. Metabolic interventions such as the ketogenic diet (KD) are of interest, but sustained dietary adherence may be limited.. Methods: Male C57BL/6 mice underwent cuprizone/rapamycin demyelination for 6 weeks and, after an additional 7-day rapamycin washout with continued cuprizone exposure, received 9 days of standard diet (SD), ketogenic diet (KD), or intermittent ketone ester injections (iKE) during early recovery.

    Confocal:

    Article Title: Computational identification and experimental validation of early glycinergic impairments in spinal muscular atrophy
    Article Snippet: Probes were purchased from ACD: Article in Press Scientific Reports https://doi.org/10.1038/s41598-026-74597-y Mm-Gcsh-O1-C1 (catalog #1240481-C1) and Mm-ChAT-C2 (catalog #408731-C2). .. The hybridized probe signal was acquired using a Zeiss Axio-observer Z1 inverted confocal microscope with a 25× oil objective. .. Spinal cord samples were homogenized in radioimmunoprecipitation assay buffer (RIPA lysis buffer, Merck Life Sciences, Darmstadt, Germany, ref. 20–188) supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF, Thermo Fisher Scientific, ref. 36978), 1 mM dithiothreitol (DTT, Thermo Fisher Scientific, ref. R0861), 2 mM sodium orthovanadate (Merck Life Sciences, ref. S6508) and 1 × complete Protease Inhibitor Cocktail (Merck Life Sciences, ref. 11697498001).

    Article Title: IscR-mediated morphological regulation confers virulence and stress resistance by reducing stress molecule uptake in Acinetobacter baumannii .
    Article Snippet: .. Fixed bacteria were immobilized on agarose pads and imaged using a Zeiss LSM 880 inverted confocal microscope or a Zeiss AxioObserver.Z1 inverted fluorescence microscope with a 63× oil immersion objective and a laser line of 488 nm for detection. ..

    Article Title: Early myelin-associated histological and cellular findings following ketogenic diet or ketone ester treatment in a cuprizone/rapamycin mouse model.
    Article Snippet: Background: Impaired restoration of CNS myelin contributes to progressive disability in multiple sclerosis (MS), yet current disease-modifying therapies do not directly target this biology.. Metabolic interventions such as the ketogenic diet (KD) are of interest, but sustained dietary adherence may be limited.. Methods: Male C57BL/6 mice underwent cuprizone/rapamycin demyelination for 6 weeks and, after an additional 7-day rapamycin washout with continued cuprizone exposure, received 9 days of standard diet (SD), ketogenic diet (KD), or intermittent ketone ester injections (iKE) during early recovery.

    Bacteria:

    Article Title: Photoinduced hydrogel coatings hybridized with silver nanoparticles to prevent Nitratidesulfovibrio vulgaris adhesion and bio-corrosion of aluminum substrates
    Article Snippet: At the start of the experiment, the optical density at 600 nm was 0.014, measured using a Spectronic 401 (Spectronic Instruments, Milton Roy, USA). .. Bacteria cells were stained with SYTO9 (1 μL, Invitrogen, S34854) for initial counting via epifluorescence microscopy with an Axio Observer Z1 inverted fluorescence microscope (Zeiss, Jean, Germany), yielding a concentration of 1.33×107 cells/mL. ..

    Article Title: IscR-mediated morphological regulation confers virulence and stress resistance by reducing stress molecule uptake in Acinetobacter baumannii .
    Article Snippet: .. Fixed bacteria were immobilized on agarose pads and imaged using a Zeiss LSM 880 inverted confocal microscope or a Zeiss AxioObserver.Z1 inverted fluorescence microscope with a 63× oil immersion objective and a laser line of 488 nm for detection. ..

    Counting:

    Article Title: Photoinduced hydrogel coatings hybridized with silver nanoparticles to prevent Nitratidesulfovibrio vulgaris adhesion and bio-corrosion of aluminum substrates
    Article Snippet: At the start of the experiment, the optical density at 600 nm was 0.014, measured using a Spectronic 401 (Spectronic Instruments, Milton Roy, USA). .. Bacteria cells were stained with SYTO9 (1 μL, Invitrogen, S34854) for initial counting via epifluorescence microscopy with an Axio Observer Z1 inverted fluorescence microscope (Zeiss, Jean, Germany), yielding a concentration of 1.33×107 cells/mL. ..

    Epifluorescence Microscopy:

    Article Title: Photoinduced hydrogel coatings hybridized with silver nanoparticles to prevent Nitratidesulfovibrio vulgaris adhesion and bio-corrosion of aluminum substrates
    Article Snippet: At the start of the experiment, the optical density at 600 nm was 0.014, measured using a Spectronic 401 (Spectronic Instruments, Milton Roy, USA). .. Bacteria cells were stained with SYTO9 (1 μL, Invitrogen, S34854) for initial counting via epifluorescence microscopy with an Axio Observer Z1 inverted fluorescence microscope (Zeiss, Jean, Germany), yielding a concentration of 1.33×107 cells/mL. ..

    Concentration Assay:

    Article Title: Photoinduced hydrogel coatings hybridized with silver nanoparticles to prevent Nitratidesulfovibrio vulgaris adhesion and bio-corrosion of aluminum substrates
    Article Snippet: At the start of the experiment, the optical density at 600 nm was 0.014, measured using a Spectronic 401 (Spectronic Instruments, Milton Roy, USA). .. Bacteria cells were stained with SYTO9 (1 μL, Invitrogen, S34854) for initial counting via epifluorescence microscopy with an Axio Observer Z1 inverted fluorescence microscope (Zeiss, Jean, Germany), yielding a concentration of 1.33×107 cells/mL. ..

    Spinning Disk:

    Article Title: Early myelin-associated histological and cellular findings following ketogenic diet or ketone ester treatment in a cuprizone/rapamycin mouse model.
    Article Snippet: Background: Impaired restoration of CNS myelin contributes to progressive disability in multiple sclerosis (MS), yet current disease-modifying therapies do not directly target this biology.. Metabolic interventions such as the ketogenic diet (KD) are of interest, but sustained dietary adherence may be limited.. Methods: Male C57BL/6 mice underwent cuprizone/rapamycin demyelination for 6 weeks and, after an additional 7-day rapamycin washout with continued cuprizone exposure, received 9 days of standard diet (SD), ketogenic diet (KD), or intermittent ketone ester injections (iKE) during early recovery.

    Software:

    Article Title: Early myelin-associated histological and cellular findings following ketogenic diet or ketone ester treatment in a cuprizone/rapamycin mouse model.
    Article Snippet: Background: Impaired restoration of CNS myelin contributes to progressive disability in multiple sclerosis (MS), yet current disease-modifying therapies do not directly target this biology.. Metabolic interventions such as the ketogenic diet (KD) are of interest, but sustained dietary adherence may be limited.. Methods: Male C57BL/6 mice underwent cuprizone/rapamycin demyelination for 6 weeks and, after an additional 7-day rapamycin washout with continued cuprizone exposure, received 9 days of standard diet (SD), ketogenic diet (KD), or intermittent ketone ester injections (iKE) during early recovery.



    Similar Products

    96
    Carl Zeiss axioobserver z1 inverted fluorescence microscope
    Axioobserver Z1 Inverted Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioobserver+fluorescence+microscope/Inverted+microscope+Axio+Observer+3+materials/10__1038_slash_s41536___026___00515___5-283-57-62
    Average 96 stars, based on 1 article reviews
    axioobserver z1 inverted fluorescence microscope - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    96
    Carl Zeiss axioobserver 7 inverted fluorescence microscope
    Axioobserver 7 Inverted Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioobserver+fluorescence+microscope/Inverted+microscope+Axio+Observer+7+materials/bio_rxiv__64898__2026__09__26__754727-209-39-38
    Average 96 stars, based on 1 article reviews
    axioobserver 7 inverted fluorescence microscope - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    99
    Carl Zeiss axioobserver z1 inverted fluorescent microscope
    Axioobserver Z1 Inverted Fluorescent Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioobserver+fluorescence+microscope/Inverted+microscope+Axio+Observer+3/10__1021_slash_acsomega__5c13065-129-9-14
    Average 99 stars, based on 1 article reviews
    axioobserver z1 inverted fluorescent microscope - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    96
    Carl Zeiss axioobserver 100 inverted fluorescence microscope
    Axioobserver 100 Inverted Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioobserver+fluorescence+microscope/Inverted+microscope+Axio+Observer+5/pmc12663426-326-19-24
    Average 96 stars, based on 1 article reviews
    axioobserver 100 inverted fluorescence microscope - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    90
    Carl Zeiss fluorescence microscope axioobserver 20× objective, axiocam 503 mono, filter cube for red dyes
    Fluorescence Microscope Axioobserver 20× Objective, Axiocam 503 Mono, Filter Cube For Red Dyes, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioobserver+fluorescence+microscope/axiocam+camera+mrm/pmc12270789-96-11-14
    Average 90 stars, based on 1 article reviews
    fluorescence microscope axioobserver 20× objective, axiocam 503 mono, filter cube for red dyes - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Carl Zeiss carl zeiss axioobserver inverted fluorescence microscope
    Carl Zeiss Axioobserver Inverted Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioobserver+fluorescence+microscope/axio+microscope+observer+z1+%CE%B2/pmc12269345-59-10-8
    Average 90 stars, based on 1 article reviews
    carl zeiss axioobserver inverted fluorescence microscope - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Carl Zeiss axioobserver d1 fluorescence microscope
    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging <t>Microscope</t> with transillumination ( Trans ) or <t>fluorescence</t> illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.
    Axioobserver D1 Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioobserver+fluorescence+microscope/axio+microscope+observer+z1+%CE%B2/bio_rxiv__2025__07__11__664221-190-10-9
    Average 90 stars, based on 1 article reviews
    axioobserver d1 fluorescence microscope - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Carl Zeiss inverted axioobserver colibri fluorescent microscope
    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging <t>Microscope</t> with transillumination ( Trans ) or <t>fluorescence</t> illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.
    Inverted Axioobserver Colibri Fluorescent Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioobserver+fluorescence+microscope/colibri+microscope/pm40619066-232-5-4
    Average 90 stars, based on 1 article reviews
    inverted axioobserver colibri fluorescent microscope - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    Image Search Results


    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging Microscope with transillumination ( Trans ) or fluorescence illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.

    Journal: bioRxiv

    Article Title: Membrane-anchored PrP Sc is the trigger for prion synaptotoxicity

    doi: 10.1101/2025.07.11.664221

    Figure Lengend Snippet: (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging Microscope with transillumination ( Trans ) or fluorescence illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.

    Article Snippet: 10-15 neurons were randomly selected and imaged using a Zeiss AxioObserver D1 Fluorescence Microscope and/or a Zeiss LSM 700 Laser Scanning Confocal Microscope with 63X oil objectives.

    Techniques: Control, Expressing, Live Cell Imaging, Microscopy, Fluorescence, Transduction, Staining, Comparison