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Merck KGaA l-15 medium l-glutamine
L 15 Medium L Glutamine, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/axioobserver+d1+microscope/leibovitz%E2%80%99s+l+15+medium+l+glutamine/pmc09687703-62-9-10
Average 90 stars, based on 1 article reviews
l-15 medium l-glutamine - by Bioz Stars, 2026-09
90/100 stars

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Saline:

Article Title: An exploratory study of cell stiffness as a mechanical label-free biomarker across multiple musculoskeletal sarcoma cells.
Article Snippet: Cell stiffness assessment - atomic force microscopy All cell types were seeded at a density of 3 × 104 in petri dishes (TPP Techno Plastic Products AG, Trasadingen, Switzerland) and covered with Leibovitz’s L-15 medium w/o l-glutamine (Merck KGaA, Darmstadt, Germany) media.

Article Title: 5-Aminolevulinic Acid-Mediated Photodynamic Therapy Potentiates the Effectiveness of Doxorubicin in Ewing Sarcomas
Article Snippet: Briefly, measurements were performed in Leibovitz’s L-15 medium without L-glutamine (Merck KGaA, Darmstadt, Germany) using a CellHesion200 (Bruker, Billerica, MA, USA) AFM system, mounted onto an inverted light microscope (AxioObserver D1; Carl Zeiss Microscopy, Jena, Germany).

Article Title: Biomechanical and biochemical assessment of YB-1 expression in A375 melanoma cell line: Exploratory study
Article Snippet: Prior to mechanical measurements, the culture medium was removed, and the cells were covered with Leibovitz’s L-15 medium without L-glutamine (Merck KGaA).

Article Title: Changes in stiffness of the extracellular and pericellular matrix in the anulus fibrosus of lumbar intervertebral discs over the course of degeneration
Article Snippet: After rinsing them again with phosphate-buffered saline, they were dyed with a green nucleic acid stain (Sytox Green, ThermoFisher Scientific, Waltham, MA, United States) at a final concentration of 1 μM and covered with Leibovitz’s L-15 medium without L-glutamine (Merck KGaA, Darmstadt, Germany) for the measurements.

Article Title: Mesenchymal Stem Cell Plasticity: What Role Do Culture Conditions and Substrates Play in Shaping Biomechanical Signatures?
Article Snippet: Prior to AFM measurements, the cells were covered with Leibovitz’s L-15 medium without l-glutamine (Merck KGaA, Darmstadt, Germany), and the AFM microindentation measurements were performed at 37 ◦C.

Article Title: Biomechanical and biochemical assessment of YB-1 expression in A375 melanoma cell line: Exploratory study
Article Snippet: The calibration of the cantilever was done on an empty Petri dish filled with pre-warmed to 37°C Leibovitz’s L-15 medium without L-glutamine (Merck KGaA, Darmstadt, Germany).

Article Title: Biomechanical and biochemical assessment of YB-1 expression in A375 melanoma cell line: Exploratory study
Article Snippet: The calibration of the cantilever was done on an empty Petri dish filled with pre-warmed to 37°C Leibovitz’s L-15 medium without L-glutamine (Merck KGaA, Darmstadt, Germany).

Article Title: Mesenchymal Stem Cell Plasticity: What Role Do Culture Conditions and Substrates Play in Shaping Biomechanical Signatures?
Article Snippet: Prior to AFM measurements, the cells were covered with Leibovitz’s L-15 medium without l-glutamine (Merck KGaA, Darmstadt, Germany), and the AFM microindentation measurements were performed at 37 °C.

Staining:

Article Title: An exploratory study of cell stiffness as a mechanical label-free biomarker across multiple musculoskeletal sarcoma cells.
Article Snippet: Cell stiffness assessment - atomic force microscopy All cell types were seeded at a density of 3 × 104 in petri dishes (TPP Techno Plastic Products AG, Trasadingen, Switzerland) and covered with Leibovitz’s L-15 medium w/o l-glutamine (Merck KGaA, Darmstadt, Germany) media.

Article Title: 5-Aminolevulinic Acid-Mediated Photodynamic Therapy Potentiates the Effectiveness of Doxorubicin in Ewing Sarcomas
Article Snippet: Briefly, measurements were performed in Leibovitz’s L-15 medium without L-glutamine (Merck KGaA, Darmstadt, Germany) using a CellHesion200 (Bruker, Billerica, MA, USA) AFM system, mounted onto an inverted light microscope (AxioObserver D1; Carl Zeiss Microscopy, Jena, Germany).

Article Title: Biomechanical and biochemical assessment of YB-1 expression in A375 melanoma cell line: Exploratory study
Article Snippet: Prior to mechanical measurements, the culture medium was removed, and the cells were covered with Leibovitz’s L-15 medium without L-glutamine (Merck KGaA).

Article Title: Changes in stiffness of the extracellular and pericellular matrix in the anulus fibrosus of lumbar intervertebral discs over the course of degeneration
Article Snippet: After rinsing them again with phosphate-buffered saline, they were dyed with a green nucleic acid stain (Sytox Green, ThermoFisher Scientific, Waltham, MA, United States) at a final concentration of 1 μM and covered with Leibovitz’s L-15 medium without L-glutamine (Merck KGaA, Darmstadt, Germany) for the measurements.

Article Title: Mesenchymal Stem Cell Plasticity: What Role Do Culture Conditions and Substrates Play in Shaping Biomechanical Signatures?
Article Snippet: Prior to AFM measurements, the cells were covered with Leibovitz’s L-15 medium without l-glutamine (Merck KGaA, Darmstadt, Germany), and the AFM microindentation measurements were performed at 37 ◦C.

Article Title: Biomechanical and biochemical assessment of YB-1 expression in A375 melanoma cell line: Exploratory study
Article Snippet: The calibration of the cantilever was done on an empty Petri dish filled with pre-warmed to 37°C Leibovitz’s L-15 medium without L-glutamine (Merck KGaA, Darmstadt, Germany).

Article Title: Biomechanical and biochemical assessment of YB-1 expression in A375 melanoma cell line: Exploratory study
Article Snippet: The calibration of the cantilever was done on an empty Petri dish filled with pre-warmed to 37°C Leibovitz’s L-15 medium without L-glutamine (Merck KGaA, Darmstadt, Germany).

Article Title: Mesenchymal Stem Cell Plasticity: What Role Do Culture Conditions and Substrates Play in Shaping Biomechanical Signatures?
Article Snippet: Prior to AFM measurements, the cells were covered with Leibovitz’s L-15 medium without l-glutamine (Merck KGaA, Darmstadt, Germany), and the AFM microindentation measurements were performed at 37 °C.

Concentration Assay:

Article Title: An exploratory study of cell stiffness as a mechanical label-free biomarker across multiple musculoskeletal sarcoma cells.
Article Snippet: Cell stiffness assessment - atomic force microscopy All cell types were seeded at a density of 3 × 104 in petri dishes (TPP Techno Plastic Products AG, Trasadingen, Switzerland) and covered with Leibovitz’s L-15 medium w/o l-glutamine (Merck KGaA, Darmstadt, Germany) media.

Article Title: 5-Aminolevulinic Acid-Mediated Photodynamic Therapy Potentiates the Effectiveness of Doxorubicin in Ewing Sarcomas
Article Snippet: Briefly, measurements were performed in Leibovitz’s L-15 medium without L-glutamine (Merck KGaA, Darmstadt, Germany) using a CellHesion200 (Bruker, Billerica, MA, USA) AFM system, mounted onto an inverted light microscope (AxioObserver D1; Carl Zeiss Microscopy, Jena, Germany).

Article Title: Biomechanical and biochemical assessment of YB-1 expression in A375 melanoma cell line: Exploratory study
Article Snippet: Prior to mechanical measurements, the culture medium was removed, and the cells were covered with Leibovitz’s L-15 medium without L-glutamine (Merck KGaA).

Article Title: Changes in stiffness of the extracellular and pericellular matrix in the anulus fibrosus of lumbar intervertebral discs over the course of degeneration
Article Snippet: After rinsing them again with phosphate-buffered saline, they were dyed with a green nucleic acid stain (Sytox Green, ThermoFisher Scientific, Waltham, MA, United States) at a final concentration of 1 μM and covered with Leibovitz’s L-15 medium without L-glutamine (Merck KGaA, Darmstadt, Germany) for the measurements.

Article Title: Mesenchymal Stem Cell Plasticity: What Role Do Culture Conditions and Substrates Play in Shaping Biomechanical Signatures?
Article Snippet: Prior to AFM measurements, the cells were covered with Leibovitz’s L-15 medium without l-glutamine (Merck KGaA, Darmstadt, Germany), and the AFM microindentation measurements were performed at 37 ◦C.

Article Title: Biomechanical and biochemical assessment of YB-1 expression in A375 melanoma cell line: Exploratory study
Article Snippet: The calibration of the cantilever was done on an empty Petri dish filled with pre-warmed to 37°C Leibovitz’s L-15 medium without L-glutamine (Merck KGaA, Darmstadt, Germany).

Article Title: Biomechanical and biochemical assessment of YB-1 expression in A375 melanoma cell line: Exploratory study
Article Snippet: The calibration of the cantilever was done on an empty Petri dish filled with pre-warmed to 37°C Leibovitz’s L-15 medium without L-glutamine (Merck KGaA, Darmstadt, Germany).

Article Title: Mesenchymal Stem Cell Plasticity: What Role Do Culture Conditions and Substrates Play in Shaping Biomechanical Signatures?
Article Snippet: Prior to AFM measurements, the cells were covered with Leibovitz’s L-15 medium without l-glutamine (Merck KGaA, Darmstadt, Germany), and the AFM microindentation measurements were performed at 37 °C.



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(A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging <t>Microscope</t> with transillumination ( Trans ) or <t>fluorescence</t> illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.
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Image Search Results


(A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging Microscope with transillumination ( Trans ) or fluorescence illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.

Journal: bioRxiv

Article Title: Membrane-anchored PrP Sc is the trigger for prion synaptotoxicity

doi: 10.1101/2025.07.11.664221

Figure Lengend Snippet: (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging Microscope with transillumination ( Trans ) or fluorescence illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.

Article Snippet: 10-15 neurons were randomly selected and imaged using a Zeiss AxioObserver D1 Fluorescence Microscope and/or a Zeiss LSM 700 Laser Scanning Confocal Microscope with 63X oil objectives.

Techniques: Control, Expressing, Live Cell Imaging, Microscopy, Fluorescence, Transduction, Staining, Comparison