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afm system  (Bruker Corporation)


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    Bruker Corporation afm system
    Representative images indicating the regions of interest subjected to elasticity measurements via <t>AFM.</t> Microscopic pictures of AFM-measured porcine adipose tissue-derived stromal cells (pADSCs) control monolayers (left pictures) and pADSCs injected by WN (middle pictures) or WJ (right pictures). Following WN and WJ injection, cells were collected, washed by PBS, and allowed to attach for 3 h in expansion medium before AFM measurements. As controls, cells that were not subjected to an injection procedure were used. The cantilever employed for measurements is also displayed (white star). Representative regions containing cells subjected to AFM measurements are also displayed (white squares). Images were acquired with <t>the</t> <t>inverted</t> AxioObserver D1 light microscope attached to the AFM system at a 10× magnification. Scale bar (black) represents 100 µm. Abbreviations: WJ—waterjet, WN—Williams needle.
    Afm System, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioobserver+d1+microscope/pmc08070533-166-2-6?v=Bruker+Corporation
    Average 96 stars, based on 1 article reviews
    afm system - by Bioz Stars, 2026-08
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    Images

    1) Product Images from "Injection of Porcine Adipose Tissue-Derived Stromal Cells by a Novel Waterjet Technology"

    Article Title: Injection of Porcine Adipose Tissue-Derived Stromal Cells by a Novel Waterjet Technology

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms22083958

    Representative images indicating the regions of interest subjected to elasticity measurements via AFM. Microscopic pictures of AFM-measured porcine adipose tissue-derived stromal cells (pADSCs) control monolayers (left pictures) and pADSCs injected by WN (middle pictures) or WJ (right pictures). Following WN and WJ injection, cells were collected, washed by PBS, and allowed to attach for 3 h in expansion medium before AFM measurements. As controls, cells that were not subjected to an injection procedure were used. The cantilever employed for measurements is also displayed (white star). Representative regions containing cells subjected to AFM measurements are also displayed (white squares). Images were acquired with the inverted AxioObserver D1 light microscope attached to the AFM system at a 10× magnification. Scale bar (black) represents 100 µm. Abbreviations: WJ—waterjet, WN—Williams needle.
    Figure Legend Snippet: Representative images indicating the regions of interest subjected to elasticity measurements via AFM. Microscopic pictures of AFM-measured porcine adipose tissue-derived stromal cells (pADSCs) control monolayers (left pictures) and pADSCs injected by WN (middle pictures) or WJ (right pictures). Following WN and WJ injection, cells were collected, washed by PBS, and allowed to attach for 3 h in expansion medium before AFM measurements. As controls, cells that were not subjected to an injection procedure were used. The cantilever employed for measurements is also displayed (white star). Representative regions containing cells subjected to AFM measurements are also displayed (white squares). Images were acquired with the inverted AxioObserver D1 light microscope attached to the AFM system at a 10× magnification. Scale bar (black) represents 100 µm. Abbreviations: WJ—waterjet, WN—Williams needle.

    Techniques Used: Derivative Assay, Control, Injection, Light Microscopy



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    Image Search Results


    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging Microscope with transillumination ( Trans ) or fluorescence illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.

    Journal: bioRxiv

    Article Title: Membrane-anchored PrP Sc is the trigger for prion synaptotoxicity

    doi: 10.1101/2025.07.11.664221

    Figure Lengend Snippet: (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging Microscope with transillumination ( Trans ) or fluorescence illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.

    Article Snippet: 10-15 neurons were randomly selected and imaged using a Zeiss AxioObserver D1 Fluorescence Microscope and/or a Zeiss LSM 700 Laser Scanning Confocal Microscope with 63X oil objectives.

    Techniques: Control, Expressing, Live Cell Imaging, Microscopy, Fluorescence, Transduction, Staining, Comparison