Review



afm system cellhesion 200  (JPK Instruments AG)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    JPK Instruments AG afm system cellhesion 200
    Detection <t>of</t> <t>JPCs</t> mineralization (Alizarin Red staining) in <t>AFM</t> and Raman petri dishes under FCS and hPL supplementation. Macroscopic images: JPC mineralization from three donors (#1, #2, #3) cultured in AFM (plastic bottom) and Raman (glass bottom) petri dishes was observed after osteogenic induction for 25 days under both medium supplementations ( A ). Quantification of JPC mineralization degrees in cell monolayers from the three donors ( B ). Calcium levels detected in cell monolayers cultured in AFM dishes are represented by red columns and those in Raman petri dishes by blue columns. Abbreviations: AFM—atomic force microscopy, FCS—fetal calf serum, hPL—human platelet lysate, * p < 0.05.
    Afm System Cellhesion 200, supplied by JPK Instruments AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioobserver+d1+microscope/afm+cellhesion+200/pmc06747376-162-13-17
    Average 90 stars, based on 1 article reviews
    afm system cellhesion 200 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Quality Analysis of Minerals Formed by Jaw Periosteal Cells under Different Culture Conditions"

    Article Title: Quality Analysis of Minerals Formed by Jaw Periosteal Cells under Different Culture Conditions

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms20174193

    Detection of JPCs mineralization (Alizarin Red staining) in AFM and Raman petri dishes under FCS and hPL supplementation. Macroscopic images: JPC mineralization from three donors (#1, #2, #3) cultured in AFM (plastic bottom) and Raman (glass bottom) petri dishes was observed after osteogenic induction for 25 days under both medium supplementations ( A ). Quantification of JPC mineralization degrees in cell monolayers from the three donors ( B ). Calcium levels detected in cell monolayers cultured in AFM dishes are represented by red columns and those in Raman petri dishes by blue columns. Abbreviations: AFM—atomic force microscopy, FCS—fetal calf serum, hPL—human platelet lysate, * p < 0.05.
    Figure Legend Snippet: Detection of JPCs mineralization (Alizarin Red staining) in AFM and Raman petri dishes under FCS and hPL supplementation. Macroscopic images: JPC mineralization from three donors (#1, #2, #3) cultured in AFM (plastic bottom) and Raman (glass bottom) petri dishes was observed after osteogenic induction for 25 days under both medium supplementations ( A ). Quantification of JPC mineralization degrees in cell monolayers from the three donors ( B ). Calcium levels detected in cell monolayers cultured in AFM dishes are represented by red columns and those in Raman petri dishes by blue columns. Abbreviations: AFM—atomic force microscopy, FCS—fetal calf serum, hPL—human platelet lysate, * p < 0.05.

    Techniques Used: Staining, Cell Culture, Microscopy

    Representative microscopic images indicating the regions of interest subjected to elasticity measurements. Microscopic pictures of AFM measured JPCs—control monolayers (left pictures), osteogenic induced JPCs (middle) and calcium phosphate precipitates (right pictures) formed under hPL and FCS culturing conditions, respectively. Red arrows depict the position of the cantilever for elasticity measurements of non-mineralized regions in untreated (left) and osteogenic monolayers (middle, without precipitates) and of calcium phosphate precipitates. Images were acquired with the inverted AxioObserver D1 light microscope attached to the AFM system at a 10× magnification. Scale bar (black) represents 30 µm. Abbreviations: AFM—atomic force microscopy, JPCs—jaw periosteum derived progenitor cells, FCS—fetal calf serum, hPL—human platelet lysate.
    Figure Legend Snippet: Representative microscopic images indicating the regions of interest subjected to elasticity measurements. Microscopic pictures of AFM measured JPCs—control monolayers (left pictures), osteogenic induced JPCs (middle) and calcium phosphate precipitates (right pictures) formed under hPL and FCS culturing conditions, respectively. Red arrows depict the position of the cantilever for elasticity measurements of non-mineralized regions in untreated (left) and osteogenic monolayers (middle, without precipitates) and of calcium phosphate precipitates. Images were acquired with the inverted AxioObserver D1 light microscope attached to the AFM system at a 10× magnification. Scale bar (black) represents 30 µm. Abbreviations: AFM—atomic force microscopy, JPCs—jaw periosteum derived progenitor cells, FCS—fetal calf serum, hPL—human platelet lysate.

    Techniques Used: Control, Light Microscopy, Microscopy, Derivative Assay

    Related Articles

    Microscopy:

    Article Title: Vinculin is required for neuronal mechanosensing but not for axon outgrowth.
    Article Snippet: Elasticity measurements were done on 3, 5, 6, 10, 15, 20, 25 and 30% of PAA gel and performed with an AFM (Nanowizard, CellHesion 200; JPK Instruments, Berlin, Germany).

    Article Title: Mechanical stimulation and electrophysiological monitoring at subcellular resolution reveals differential mechanosensation of neurons within networks.
    Article Snippet: An AFM (CellHesion 200; JPK Instruments) was mounted onto the confocal microscope.

    Article Title: Cortical cell stiffness is independent of substrate mechanics
    Article Snippet: AFM measurements were performed on JPK Cellhesion 200 AFMs (JPK Instruments AG) installed either on a conventional (Axio Observer.A1, Carl Zeiss Ltd., Cambridge, UK) or a confocal optical microscope (see below).

    Article Title: Homing of mRNA-Modified Endothelial Progenitor Cells to Inflamed Endothelium
    Article Snippet: The cantilever of the AFM (CellHesion 200, JPK Instruments, Berlin, Germany) was calibrated on the extend curve (vertical deflection), and its spring constant was determined using the thermal noise method of the data-processing software (JPK Instruments).

    Article Title: The CUL4B-based E3 ubiquitin ligase regulates mitosis and brain development by recruiting phospho-specific DCAFs.
    Article Snippet: Experiments were performed using an AFM setup (CellHesion 200; JPK Instruments) mounted on an inverted microscope (Observer Z1; Carl Zeiss Microscopy).

    Article Title: Highly specific and non-invasive imaging of Piezo1-dependent activity across scales using GenEPi.
    Article Snippet: For the mechanical stimulation, an AFM (CellHesion 200, JPK Instruments) was mounted on an inverted confocal microscope (Observer Z1, LSM 700, Zeiss).

    Article Title: Protease-activated receptor signalling initiates α 5 β 1 -integrin-mediated adhesion in non-haematopoietic cells.
    Article Snippet: 1Eidgenössische Technische Hochschule (ETH) Zurich, Department of Biosystems Science and Engineering, Basel, Switzerland.. 2Max Planck Institute of Biochemistry, Department of Molecular Medicine, Martinsried, Germany.. 3These authors contributed equally: Patrizia M. Spoerri, Nico Strohmeyer.

    Transfection:

    Article Title: Vinculin is required for neuronal mechanosensing but not for axon outgrowth.
    Article Snippet: Elasticity measurements were done on 3, 5, 6, 10, 15, 20, 25 and 30% of PAA gel and performed with an AFM (Nanowizard, CellHesion 200; JPK Instruments, Berlin, Germany).

    Article Title: Mechanical stimulation and electrophysiological monitoring at subcellular resolution reveals differential mechanosensation of neurons within networks.
    Article Snippet: An AFM (CellHesion 200; JPK Instruments) was mounted onto the confocal microscope.

    Article Title: Cortical cell stiffness is independent of substrate mechanics
    Article Snippet: AFM measurements were performed on JPK Cellhesion 200 AFMs (JPK Instruments AG) installed either on a conventional (Axio Observer.A1, Carl Zeiss Ltd., Cambridge, UK) or a confocal optical microscope (see below).

    Article Title: Homing of mRNA-Modified Endothelial Progenitor Cells to Inflamed Endothelium
    Article Snippet: The cantilever of the AFM (CellHesion 200, JPK Instruments, Berlin, Germany) was calibrated on the extend curve (vertical deflection), and its spring constant was determined using the thermal noise method of the data-processing software (JPK Instruments).

    Article Title: The CUL4B-based E3 ubiquitin ligase regulates mitosis and brain development by recruiting phospho-specific DCAFs.
    Article Snippet: Experiments were performed using an AFM setup (CellHesion 200; JPK Instruments) mounted on an inverted microscope (Observer Z1; Carl Zeiss Microscopy).

    Article Title: Highly specific and non-invasive imaging of Piezo1-dependent activity across scales using GenEPi.
    Article Snippet: For the mechanical stimulation, an AFM (CellHesion 200, JPK Instruments) was mounted on an inverted confocal microscope (Observer Z1, LSM 700, Zeiss).

    Article Title: Protease-activated receptor signalling initiates α 5 β 1 -integrin-mediated adhesion in non-haematopoietic cells.
    Article Snippet: 1Eidgenössische Technische Hochschule (ETH) Zurich, Department of Biosystems Science and Engineering, Basel, Switzerland.. 2Max Planck Institute of Biochemistry, Department of Molecular Medicine, Martinsried, Germany.. 3These authors contributed equally: Patrizia M. Spoerri, Nico Strohmeyer.

    Clinical Proteomics:

    Article Title: Vinculin is required for neuronal mechanosensing but not for axon outgrowth.
    Article Snippet: Elasticity measurements were done on 3, 5, 6, 10, 15, 20, 25 and 30% of PAA gel and performed with an AFM (Nanowizard, CellHesion 200; JPK Instruments, Berlin, Germany).

    Article Title: Mechanical stimulation and electrophysiological monitoring at subcellular resolution reveals differential mechanosensation of neurons within networks.
    Article Snippet: An AFM (CellHesion 200; JPK Instruments) was mounted onto the confocal microscope.

    Article Title: Cortical cell stiffness is independent of substrate mechanics
    Article Snippet: AFM measurements were performed on JPK Cellhesion 200 AFMs (JPK Instruments AG) installed either on a conventional (Axio Observer.A1, Carl Zeiss Ltd., Cambridge, UK) or a confocal optical microscope (see below).

    Article Title: Homing of mRNA-Modified Endothelial Progenitor Cells to Inflamed Endothelium
    Article Snippet: The cantilever of the AFM (CellHesion 200, JPK Instruments, Berlin, Germany) was calibrated on the extend curve (vertical deflection), and its spring constant was determined using the thermal noise method of the data-processing software (JPK Instruments).

    Article Title: The CUL4B-based E3 ubiquitin ligase regulates mitosis and brain development by recruiting phospho-specific DCAFs.
    Article Snippet: Experiments were performed using an AFM setup (CellHesion 200; JPK Instruments) mounted on an inverted microscope (Observer Z1; Carl Zeiss Microscopy).

    Article Title: Highly specific and non-invasive imaging of Piezo1-dependent activity across scales using GenEPi.
    Article Snippet: For the mechanical stimulation, an AFM (CellHesion 200, JPK Instruments) was mounted on an inverted confocal microscope (Observer Z1, LSM 700, Zeiss).

    Article Title: Protease-activated receptor signalling initiates α 5 β 1 -integrin-mediated adhesion in non-haematopoietic cells.
    Article Snippet: 1Eidgenössische Technische Hochschule (ETH) Zurich, Department of Biosystems Science and Engineering, Basel, Switzerland.. 2Max Planck Institute of Biochemistry, Department of Molecular Medicine, Martinsried, Germany.. 3These authors contributed equally: Patrizia M. Spoerri, Nico Strohmeyer.



    Similar Products

    axioobserver d1 fluorescence microscope  (Carl Zeiss)
    90
    Carl Zeiss axioobserver d1 fluorescence microscope
    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging <t>Microscope</t> with transillumination ( Trans ) or <t>fluorescence</t> illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.
    Axioobserver D1 Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioobserver+d1+microscope/axio+microscope+observer+z1+%CE%B2/bio_rxiv__2025__07__11__664221-190-10-9
    Average 90 stars, based on 1 article reviews
    axioobserver d1 fluorescence microscope - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    lens-type total internal reflection (tirf) microscopes based on axioobserver d1 microscope with an x100/1.46 objective  (Carl Zeiss)
    90
    Carl Zeiss lens-type total internal reflection (tirf) microscopes based on axioobserver d1 microscope with an x100/1.46 objective
    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging <t>Microscope</t> with transillumination ( Trans ) or <t>fluorescence</t> illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.
    Lens Type Total Internal Reflection (Tirf) Microscopes Based On Axioobserver D1 Microscope With An X100/1.46 Objective, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioobserver+d1+microscope/135+axiovert+microscope/pm40651941-627-24-31
    Average 90 stars, based on 1 article reviews
    lens-type total internal reflection (tirf) microscopes based on axioobserver d1 microscope with an x100/1.46 objective - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    microscope zeiss axioobserver d1  (Carl Zeiss)
    90
    Carl Zeiss microscope zeiss axioobserver d1
    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging <t>Microscope</t> with transillumination ( Trans ) or <t>fluorescence</t> illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.
    Microscope Zeiss Axioobserver D1, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioobserver+d1+microscope/axio+microscope+observer+z1+%CE%B2/pm40644788-121-9-8
    Average 90 stars, based on 1 article reviews
    microscope zeiss axioobserver d1 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    axioobserver d1 inverted fluorescence microscope  (Carl Zeiss)
    90
    Carl Zeiss axioobserver d1 inverted fluorescence microscope
    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging <t>Microscope</t> with transillumination ( Trans ) or <t>fluorescence</t> illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.
    Axioobserver D1 Inverted Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioobserver+d1+microscope/axio+microscope+observer+z1+%CE%B2/pm40497733-243-13-18
    Average 90 stars, based on 1 article reviews
    axioobserver d1 inverted fluorescence microscope - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    microscope axioobserver d1  (Carl Zeiss)
    90
    Carl Zeiss microscope axioobserver d1
    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging <t>Microscope</t> with transillumination ( Trans ) or <t>fluorescence</t> illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.
    Microscope Axioobserver D1, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioobserver+d1+microscope/axio+microscope+observer+z1+%CE%B2/pm40344932-51-14-17
    Average 90 stars, based on 1 article reviews
    microscope axioobserver d1 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    zeiss axioobserver z1 7 microscope  (Carl Zeiss)
    97
    Carl Zeiss zeiss axioobserver z1 7 microscope
    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging <t>Microscope</t> with transillumination ( Trans ) or <t>fluorescence</t> illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.
    Zeiss Axioobserver Z1 7 Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioobserver+d1+microscope/bio_rxiv__2025__03__14__643060-374-30-30
    Average 97 stars, based on 1 article reviews
    zeiss axioobserver z1 7 microscope - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier
    axioobserver d1 microscope  (Carl Zeiss)
    90
    Carl Zeiss axioobserver d1 microscope
    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging <t>Microscope</t> with transillumination ( Trans ) or <t>fluorescence</t> illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.
    Axioobserver D1 Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioobserver+d1+microscope/axio+microscope+observer+z1+%CE%B2/pm39667069-45-7-10
    Average 90 stars, based on 1 article reviews
    axioobserver d1 microscope - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging Microscope with transillumination ( Trans ) or fluorescence illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.

    Journal: bioRxiv

    Article Title: Membrane-anchored PrP Sc is the trigger for prion synaptotoxicity

    doi: 10.1101/2025.07.11.664221

    Figure Lengend Snippet: (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging Microscope with transillumination ( Trans ) or fluorescence illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.

    Article Snippet: 10-15 neurons were randomly selected and imaged using a Zeiss AxioObserver D1 Fluorescence Microscope and/or a Zeiss LSM 700 Laser Scanning Confocal Microscope with 63X oil objectives.

    Techniques: Control, Expressing, Live Cell Imaging, Microscopy, Fluorescence, Transduction, Staining, Comparison