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axiocam 503 mono camera  (Carl Zeiss)


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    Structured Review

    Carl Zeiss axiocam 503 mono camera
    Axiocam 503 Mono Camera, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 639 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioobserver+d1+microscope/Microscopy+Camera+Axiocam+503+mono+(D)/pm41596698-195-66-70
    Average 96 stars, based on 639 article reviews
    axiocam 503 mono camera - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Region and Cell-Selective Induction of Zbtb16/Plzf by Multiple Stressors in the Adult Murine Hypothalamus.
    Article Snippet: .. Images were acquired using either a Zeiss LSM 880 or Zeiss LSM 800 confocal microscope, both equipped with Axiocam 503 mono cameras (Carl Zeiss Inc.) and 40×/0.95 Corr PlanApochromat oil- immersion objectives. ..

    Article Title: Plug-and-play evolution of the Klebsiella pneumoniae capsule locus enables serotype exchange across genetic backgrounds.
    Article Snippet: .. Cell morphologies were imaged using a Zeiss Axio Imager.M2 microscope, with ×100 magnification, equipped with an Axiocam 503 mono camera (Carl Zeiss, Germany). ..

    Article Title: Loss of DIAPH3 accelerates glioma genesis in mice.
    Article Snippet: .. Images were obtained with a Zeiss Axio Vert.A1 microscope equipped with a Zeiss Axiocam 503 mono camera (Zeiss, Germany). .. Images were obtained with a Zeiss Axio Vert.A1 microscope equipped with a Zeiss Axiocam 503 mono camera (Zeiss, Germany).

    Article Title: Loss of DIAPH3 accelerates glioma genesis in mice
    Article Snippet: .. Images were obtained with a Zeiss Axio Vert.A1 microscope equipped with a Zeiss Axiocam 503 mono camera (Zeiss, Germany). ..

    Article Title: Region and Cell‐Selective Induction of Zbtb16/Plzf by Multiple Stressors in the Adult Murine Hypothalamus
    Article Snippet: .. Images were acquired using either a Zeiss LSM 880 or Zeiss LSM 800 confocal microscope, both equipped with Axiocam 503 mono cameras (Carl Zeiss Inc.) and 40×/0.95 Corr Plan‐Apochromat oil‐immersion objectives. ..

    Article Title: AxoMetric platform facilitates rapid and unbiased automated quantification of axon regeneration in tissue sections
    Article Snippet: The following day retinas were rinsed 3x with PBS for 30 min each and incubated with secondary antibodies (Donkey anti-Rabbit 488 Jackson ImmunoResearch Labs, #711-545-152, RRID: AB_2313584 , 1:200) overnight at 4°C. .. Optic and sciatic nerve sections were imaged using the Zeiss Axio Observer Z1 inverted fluorescence microscope fitted with a Zeiss Axiocam 503 mono camera and Zen 3.9 software. ..

    Article Title: Plug-and-play evolution of the Klebsiella pneumoniae capsule locus enables serotype exchange across genetic backgrounds
    Article Snippet: .. Cell morphologies were imaged using a Zeiss Axio Imager.M2 microscope, with ×100 magnification, equipped with an Axiocam 503 mono camera (Carl Zeiss, Germany). ..

    Article Title: Intrinsic mechanisms and microenvironmental cues fine-tune plasticity of esophageal progenitors.
    Article Snippet: .. Imaging was performed on a Zeiss Axio Imager M2 fluorescence microscope with a Zeiss Axiocam 503 mono camera for immunofluorescence microscopy using Zen Blue 3.2 (Zeiss) software. ..

    Fluorescence:

    Article Title: AxoMetric platform facilitates rapid and unbiased automated quantification of axon regeneration in tissue sections
    Article Snippet: The following day retinas were rinsed 3x with PBS for 30 min each and incubated with secondary antibodies (Donkey anti-Rabbit 488 Jackson ImmunoResearch Labs, #711-545-152, RRID: AB_2313584 , 1:200) overnight at 4°C. .. Optic and sciatic nerve sections were imaged using the Zeiss Axio Observer Z1 inverted fluorescence microscope fitted with a Zeiss Axiocam 503 mono camera and Zen 3.9 software. ..

    Article Title: Intrinsic mechanisms and microenvironmental cues fine-tune plasticity of esophageal progenitors.
    Article Snippet: .. Imaging was performed on a Zeiss Axio Imager M2 fluorescence microscope with a Zeiss Axiocam 503 mono camera for immunofluorescence microscopy using Zen Blue 3.2 (Zeiss) software. ..

    Software:

    Article Title: AxoMetric platform facilitates rapid and unbiased automated quantification of axon regeneration in tissue sections
    Article Snippet: The following day retinas were rinsed 3x with PBS for 30 min each and incubated with secondary antibodies (Donkey anti-Rabbit 488 Jackson ImmunoResearch Labs, #711-545-152, RRID: AB_2313584 , 1:200) overnight at 4°C. .. Optic and sciatic nerve sections were imaged using the Zeiss Axio Observer Z1 inverted fluorescence microscope fitted with a Zeiss Axiocam 503 mono camera and Zen 3.9 software. ..

    Imaging:

    Article Title: Intrinsic mechanisms and microenvironmental cues fine-tune plasticity of esophageal progenitors.
    Article Snippet: .. Imaging was performed on a Zeiss Axio Imager M2 fluorescence microscope with a Zeiss Axiocam 503 mono camera for immunofluorescence microscopy using Zen Blue 3.2 (Zeiss) software. ..

    Immunofluorescence:

    Article Title: Intrinsic mechanisms and microenvironmental cues fine-tune plasticity of esophageal progenitors.
    Article Snippet: .. Imaging was performed on a Zeiss Axio Imager M2 fluorescence microscope with a Zeiss Axiocam 503 mono camera for immunofluorescence microscopy using Zen Blue 3.2 (Zeiss) software. ..



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    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging <t>Microscope</t> with transillumination ( Trans ) or <t>fluorescence</t> illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.
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    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging <t>Microscope</t> with transillumination ( Trans ) or <t>fluorescence</t> illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.
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    Image Search Results


    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging Microscope with transillumination ( Trans ) or fluorescence illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.

    Journal: bioRxiv

    Article Title: Membrane-anchored PrP Sc is the trigger for prion synaptotoxicity

    doi: 10.1101/2025.07.11.664221

    Figure Lengend Snippet: (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging Microscope with transillumination ( Trans ) or fluorescence illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.

    Article Snippet: 10-15 neurons were randomly selected and imaged using a Zeiss AxioObserver D1 Fluorescence Microscope and/or a Zeiss LSM 700 Laser Scanning Confocal Microscope with 63X oil objectives.

    Techniques: Control, Expressing, Live Cell Imaging, Microscopy, Fluorescence, Transduction, Staining, Comparison