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lens-type total internal reflection (tirf) microscopes based on axioobserver d1 microscope with an x100/1.46 objective  (Carl Zeiss)


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    Structured Review

    Carl Zeiss lens-type total internal reflection (tirf) microscopes based on axioobserver d1 microscope with an x100/1.46 objective
    Lens Type Total Internal Reflection (Tirf) Microscopes Based On Axioobserver D1 Microscope With An X100/1.46 Objective, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioobserver+d1+microscope/135+axiovert+microscope/pm40651941-627-24-31
    Average 90 stars, based on 1 article reviews
    lens-type total internal reflection (tirf) microscopes based on axioobserver d1 microscope with an x100/1.46 objective - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Microscopy:

    Article Title: Highly adaptable deep-learning platform for automated detection and analysis of vesicle exocytosis.
    Article Snippet: .. INS1 cells (clone 832/13) that transiently expressed NPYmGFP, NPY-mNeonGreen or NPY-mCherry were imaged using a custom-built lens-type total internal reflection (TIRF) microscopes based on AxioObserver D1 microscope with an x100/1.46 objective (Carl Zeiss, Cat# 420792-9800-720). .. Excitation was from a diode laser module at 473 nm, or a diode pumped laser at 561 nm, respectively (Cobolt, Göteborg, Sweden, Cat# 0473-06-01-0300-100 & Cat# 0561- 06-91-0100-100), controlled by an acoustic-optical tunable filter (AOTF, AA-Opto, France, Cat#AOTFnC-400 650-TN).

    Article Title: Phylogenomic and Pangenomic Assessment of a Mediterranean Strain of Raphidiopsis raciborskii Extends Knowledge of the Global Distribution and Characteristics of a Potentially Toxigenic Cyanobacterium
    Article Snippet: .. Field and culture samples were observed under an invertoscope Zeiss Axiovert 135 and an upright microscope Leica DM 2500. ..

    Article Title: Phloxine B-loaded polymersomes enable eradication of Pseudomonas aeruginosa and Staphylococcus aureus in antimicrobial photodynamic therapy
    Article Snippet: .. The structures were also observed via epifluorescence microscopy, using an Axiovert 135 microscope equipped with an AxioCamMRm and Zen 2.3 lite software and a filter set with λ ex = 540–552 nm and λ em > 590 nm (Carl Zeiss MicroImaging GmbH, Germany). ..

    other:

    Article Title: Highly adaptable deep-learning platform for automated detection and analysis of vesicle exocytosis
    Article Snippet: DRG/SC co-culture was maintained in Neurobasal A (NBA) medium (Cat#° 21103049) supplemented with fetal calf serum (5% v/v, Cat# 11550356), penicillin and streptomycin (0.2% each, Cat# 11548876), B27 supplement (2%, Cat# 17504-044), GlutaMAX (1%, Cat# 35050-061, all products from Thermo Fisher Scientific, Waltham, MA, USA), and human beta-nerve growth factor (0.2 μg/mL, Cat# N245, Alomone Labs, Jerusalem, Israel) at 37 °C and 5% CO 2 .

    Article Title: Transcriptional Changes in Cumulus Cells During In Vitro Maturation of Human Oocytes.
    Article Snippet: After 42 h, COCs were mechanically denuded, as described above, and classified based on their maturation stage using an inverted microscope (Carl Zeiss Axiovert 135, Germany; × 20 magnification).

    Inverted Microscopy:

    Article Title: Direction-dependent contributions of cardiac myofilament networks to myocardial passive stiffness reveal a major disparity for titin.
    Article Snippet: Mechanical experiments used a setup (Aurora Scientific, 403A) that combines a sensitive force transducer and a piezo motor, with a cardiomyocyte suspended using Shellac glue (in 70% ethanol). .. During measurements, cells were observed under an Axiovert 135 inverted microscope (Carl Zeiss) for SL recording. ..

    Article Title: Exploration of Bromodomain Proteins as Drug Targets for Niemann-Pick Type C Disease.
    Article Snippet: Following treatment, cells were washed three times with PBS, fixed by paraformaldehyde (4% in PBS) for 15 min at room temperature and stained with filipin (50 μg/mL in PBS prepared freshly from a 250-fold ethanolic stock; #F9765, Sigma-Aldrich/Merck) for 2 h at room temperature in the dark. .. Fluorescence images of stained cells were acquired using an inverted microscope (Axiovert 135TV; Carl Zeiss Microscopy GmbH, Oberkochen, Germany) equipped with a metal halide lamp (10%; Lumen 200, Prior Scientific Instruments GmbH, Jena, Germany), an appropriate excitation/emission filter (XF02-2; Omega Optical, LLC/Laser Components S.A.S., Meudon, France), a 40× objective (oil, N.A. .. 1.3; Carl Zeiss Microscopy GmbH) and an air-cooled monochrome charge-coupled device camera (Sensicam, PCO Computer Optics, Kehlheim, Germany) controlled by custom-written LabVIEW routines (Version 6.0; National Instruments).

    Article Title: STIM1-induced widening of non-pore-lining TM interfaces is crucial for Orai1 pore opening
    Article Snippet: .. Coverslips were transferred to an extracellular solution without Ca2+ and mounted on an Axiovert 135 inverted microscope (Zeiss, Germany) equipped with a sCMOS-Panda digital Scientific Grade camera 4.2 MPixel and a LedHUB LED Light-Engine light source (LedHUB; Omicron-Laserage Laserprodukte). ..

    Fluorescence:

    Article Title: Exploration of Bromodomain Proteins as Drug Targets for Niemann-Pick Type C Disease.
    Article Snippet: Following treatment, cells were washed three times with PBS, fixed by paraformaldehyde (4% in PBS) for 15 min at room temperature and stained with filipin (50 μg/mL in PBS prepared freshly from a 250-fold ethanolic stock; #F9765, Sigma-Aldrich/Merck) for 2 h at room temperature in the dark. .. Fluorescence images of stained cells were acquired using an inverted microscope (Axiovert 135TV; Carl Zeiss Microscopy GmbH, Oberkochen, Germany) equipped with a metal halide lamp (10%; Lumen 200, Prior Scientific Instruments GmbH, Jena, Germany), an appropriate excitation/emission filter (XF02-2; Omega Optical, LLC/Laser Components S.A.S., Meudon, France), a 40× objective (oil, N.A. .. 1.3; Carl Zeiss Microscopy GmbH) and an air-cooled monochrome charge-coupled device camera (Sensicam, PCO Computer Optics, Kehlheim, Germany) controlled by custom-written LabVIEW routines (Version 6.0; National Instruments).

    Staining:

    Article Title: Exploration of Bromodomain Proteins as Drug Targets for Niemann-Pick Type C Disease.
    Article Snippet: Following treatment, cells were washed three times with PBS, fixed by paraformaldehyde (4% in PBS) for 15 min at room temperature and stained with filipin (50 μg/mL in PBS prepared freshly from a 250-fold ethanolic stock; #F9765, Sigma-Aldrich/Merck) for 2 h at room temperature in the dark. .. Fluorescence images of stained cells were acquired using an inverted microscope (Axiovert 135TV; Carl Zeiss Microscopy GmbH, Oberkochen, Germany) equipped with a metal halide lamp (10%; Lumen 200, Prior Scientific Instruments GmbH, Jena, Germany), an appropriate excitation/emission filter (XF02-2; Omega Optical, LLC/Laser Components S.A.S., Meudon, France), a 40× objective (oil, N.A. .. 1.3; Carl Zeiss Microscopy GmbH) and an air-cooled monochrome charge-coupled device camera (Sensicam, PCO Computer Optics, Kehlheim, Germany) controlled by custom-written LabVIEW routines (Version 6.0; National Instruments).

    Epifluorescence Microscopy:

    Article Title: Phloxine B-loaded polymersomes enable eradication of Pseudomonas aeruginosa and Staphylococcus aureus in antimicrobial photodynamic therapy
    Article Snippet: .. The structures were also observed via epifluorescence microscopy, using an Axiovert 135 microscope equipped with an AxioCamMRm and Zen 2.3 lite software and a filter set with λ ex = 540–552 nm and λ em > 590 nm (Carl Zeiss MicroImaging GmbH, Germany). ..

    Software:

    Article Title: Phloxine B-loaded polymersomes enable eradication of Pseudomonas aeruginosa and Staphylococcus aureus in antimicrobial photodynamic therapy
    Article Snippet: .. The structures were also observed via epifluorescence microscopy, using an Axiovert 135 microscope equipped with an AxioCamMRm and Zen 2.3 lite software and a filter set with λ ex = 540–552 nm and λ em > 590 nm (Carl Zeiss MicroImaging GmbH, Germany). ..



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    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging <t>Microscope</t> with transillumination ( Trans ) or <t>fluorescence</t> illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.
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    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging <t>Microscope</t> with transillumination ( Trans ) or <t>fluorescence</t> illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.
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    Image Search Results


    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging Microscope with transillumination ( Trans ) or fluorescence illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.

    Journal: bioRxiv

    Article Title: Membrane-anchored PrP Sc is the trigger for prion synaptotoxicity

    doi: 10.1101/2025.07.11.664221

    Figure Lengend Snippet: (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging Microscope with transillumination ( Trans ) or fluorescence illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.

    Article Snippet: 10-15 neurons were randomly selected and imaged using a Zeiss AxioObserver D1 Fluorescence Microscope and/or a Zeiss LSM 700 Laser Scanning Confocal Microscope with 63X oil objectives.

    Techniques: Control, Expressing, Live Cell Imaging, Microscopy, Fluorescence, Transduction, Staining, Comparison