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axioobserver d1 inverted fluorescence microscope  (Carl Zeiss)


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    Structured Review

    Carl Zeiss axioobserver d1 inverted fluorescence microscope
    Axioobserver D1 Inverted Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 4854 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioobserver+d1+microscope/Inverted+microscope+Axio+Observer+3/pmc04255936-167-8-7
    Average 99 stars, based on 4854 article reviews
    axioobserver d1 inverted fluorescence microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Exosome-niosome hybrid oxygen carrier for protection against acetaminophen-induced acute liver injury.
    Article Snippet: .. Dark-field images were obtained by a Zeiss Axio Observer Z1 inverted research-grade microscope with 10× magnification (Carl Zeiss AG, USA). ..

    Article Title: Exosome–niosome hybrid oxygen carrier for protection against acetaminophen-induced acute liver injury
    Article Snippet: .. Dark-field images were obtained by a Zeiss Axio Observer Z1 inverted research-grade microscope with 10× magnification (Carl Zeiss AG, USA). ..

    Article Title: Exosome–niosome hybrid oxygen carrier for protection against acetaminophen-induced acute liver injury
    Article Snippet: .. Fluorescent images were obtained by a Zeiss Axio Observer Z1 inverted research-grade microscope with 10× magnification (Carl Zeiss AG, USA). ..

    Article Title: Exosome-niosome hybrid oxygen carrier for protection against acetaminophen-induced acute liver injury.
    Article Snippet: .. Fluorescent images were obtained by a Zeiss Axio Observer Z1 inverted researchgrade microscope with 10× magnification (Carl Zeiss AG, USA). ..

    Inverted Microscopy:

    Article Title: Human pluripotent stem cell-derived atrioventricular node-like pacemaker cells exhibit biological conduction bridge properties.
    Article Snippet: .. Spontaneous beating was recorded using a Zeiss Axio Observer Z1 inverted microscope equipped with a PECON incubation chamber (37◦C, 5% CO2), operated using Zeiss Zen software. ..

    Article Title: 5′untranslated regions provide a versatile toolkit for tunable exogenous protein expression
    Article Snippet: .. Cells were then blocked in 5% BSA + 2% Glycine in PBS for 5 min, followed by incubation in primary anti-TRPA1/ANKTM1 (mouse, 1:100, Santa Cruz biotechnologies sc-376495) in 5% BSA + 2% Glycine in PBS at room temperature for 1 h. Cells were then washed with PBS and incubated with Hoechst 33342 (1:2000, Invitrogen) in 5% BSA + 2% Glycine in PBS at room temperature for 5 min. Representative images in and were acquired on a Zeiss Axio Observer Z1 inverted microscope equipped with an Airyscan detection unit, using a Plan-Apochromat 63x/1.40 NA oil objective. .. Widefield images were acquired using the ImageXpress 4.0 high-throughput fluorescent imaging system (Molecular Devices) at the Yale Center for Molecular Discovery.

    Article Title: Protocol for monitoring cytoplasmic Ca 2+ dynamics using Fura Red-AM on a Zeiss Axio Observer microscope.
    Article Snippet: .. Here, we present a protocol for live-cell calcium imaging in HEK, HeLa, fibroblast, and myoblast cells using Fura Red-AM and a Zeiss Axio Observer inverted microscope. ..

    Article Title: Magnetosome organelles are organized through interactions between McaA and McaB that alter the dynamics of the bacterial actin-like protein MamK.
    Article Snippet: .. Imaging was done on a Zeiss Axio Observer Inverted microscope with Zen software (Zeiss), and images were handled using Fiji. ..

    Incubation:

    Article Title: Human pluripotent stem cell-derived atrioventricular node-like pacemaker cells exhibit biological conduction bridge properties.
    Article Snippet: .. Spontaneous beating was recorded using a Zeiss Axio Observer Z1 inverted microscope equipped with a PECON incubation chamber (37◦C, 5% CO2), operated using Zeiss Zen software. ..

    Article Title: 5′untranslated regions provide a versatile toolkit for tunable exogenous protein expression
    Article Snippet: .. Cells were then blocked in 5% BSA + 2% Glycine in PBS for 5 min, followed by incubation in primary anti-TRPA1/ANKTM1 (mouse, 1:100, Santa Cruz biotechnologies sc-376495) in 5% BSA + 2% Glycine in PBS at room temperature for 1 h. Cells were then washed with PBS and incubated with Hoechst 33342 (1:2000, Invitrogen) in 5% BSA + 2% Glycine in PBS at room temperature for 5 min. Representative images in and were acquired on a Zeiss Axio Observer Z1 inverted microscope equipped with an Airyscan detection unit, using a Plan-Apochromat 63x/1.40 NA oil objective. .. Widefield images were acquired using the ImageXpress 4.0 high-throughput fluorescent imaging system (Molecular Devices) at the Yale Center for Molecular Discovery.

    Software:

    Article Title: Human pluripotent stem cell-derived atrioventricular node-like pacemaker cells exhibit biological conduction bridge properties.
    Article Snippet: .. Spontaneous beating was recorded using a Zeiss Axio Observer Z1 inverted microscope equipped with a PECON incubation chamber (37◦C, 5% CO2), operated using Zeiss Zen software. ..

    Article Title: Magnetosome organelles are organized through interactions between McaA and McaB that alter the dynamics of the bacterial actin-like protein MamK.
    Article Snippet: .. Imaging was done on a Zeiss Axio Observer Inverted microscope with Zen software (Zeiss), and images were handled using Fiji. ..

    Imaging:

    Article Title: Protocol for monitoring cytoplasmic Ca 2+ dynamics using Fura Red-AM on a Zeiss Axio Observer microscope.
    Article Snippet: .. Here, we present a protocol for live-cell calcium imaging in HEK, HeLa, fibroblast, and myoblast cells using Fura Red-AM and a Zeiss Axio Observer inverted microscope. ..

    Article Title: Magnetosome organelles are organized through interactions between McaA and McaB that alter the dynamics of the bacterial actin-like protein MamK.
    Article Snippet: .. Imaging was done on a Zeiss Axio Observer Inverted microscope with Zen software (Zeiss), and images were handled using Fiji. ..



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    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging <t>Microscope</t> with transillumination ( Trans ) or <t>fluorescence</t> illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.
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    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging <t>Microscope</t> with transillumination ( Trans ) or <t>fluorescence</t> illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.
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    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging <t>Microscope</t> with transillumination ( Trans ) or <t>fluorescence</t> illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.
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    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging <t>Microscope</t> with transillumination ( Trans ) or <t>fluorescence</t> illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.
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    Image Search Results


    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging Microscope with transillumination ( Trans ) or fluorescence illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.

    Journal: bioRxiv

    Article Title: Membrane-anchored PrP Sc is the trigger for prion synaptotoxicity

    doi: 10.1101/2025.07.11.664221

    Figure Lengend Snippet: (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging Microscope with transillumination ( Trans ) or fluorescence illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.

    Article Snippet: 10-15 neurons were randomly selected and imaged using a Zeiss AxioObserver D1 Fluorescence Microscope and/or a Zeiss LSM 700 Laser Scanning Confocal Microscope with 63X oil objectives.

    Techniques: Control, Expressing, Live Cell Imaging, Microscopy, Fluorescence, Transduction, Staining, Comparison