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axioobserver d1 inverted fluorescence microscope  (Carl Zeiss)


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    Structured Review

    Carl Zeiss axioobserver d1 inverted fluorescence microscope
    Axioobserver D1 Inverted Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 4911 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioobserver+d1+microscope/Inverted+microscope+Axio+Observer+3/pmc04255936-167-8-7
    Average 99 stars, based on 4911 article reviews
    axioobserver d1 inverted fluorescence microscope - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Integrated Single‐Myofibre Multi‐Omics Correlates Inflammatory State to Myosin Energetics in ICU‐Acquired Weakness
    Article Snippet: .. Simultaneously, fluorescence decay was captured using an inverted Zeiss Axio Observer microscope. ..

    Article Title: Interaction between FmRAV4 (a RAV transcription factor) and FmMYB1 (a MYB transcription factor) confers drought tolerance via ROS scavenging in Fraxinus mandshurica.
    Article Snippet: Drought stress severely restricts plant growth and development.. Although AP2/ERF family transcription factors (TFs) participate extensively in abiotic stress responses, they remain largely unexplored in Fraxinus mandshurica.. In this study, we identified and functionally characterized FmRAV4, a TF from the RAV subfamily.

    Article Title: High ex vivo recombination rate of African swine fever virus in porcine alveolar macrophages.
    Article Snippet: .. The cells were monitored and photographed using an Axio Observer D1 inverted fluorescence microscope (Carl Zeiss AG, Oberkochen, Germany). .. Red and green fluorescent signals were detected using Zeiss filter sets 31 Alexa Fluor 568 and 38 HE GFP, respectively.

    Article Title: Sex-dependent transcriptional and imaging responses to metformin in young-adult mice with SuHx-induced pulmonary arterial hypertension
    Article Snippet: Subsequently, the sections were incubated with FITC-conjugated lectin (Sigma, L-4895) diluted in PBS (100 μg/mL) for 90 min to visualise the cell membranes, as WGA specifically binds to membrane glycoproteins. .. Images were captured using an Axio Observer Z1 inverted fluorescence microscope (Zeiss). ..

    Microscopy:

    Article Title: Integrated Single‐Myofibre Multi‐Omics Correlates Inflammatory State to Myosin Energetics in ICU‐Acquired Weakness
    Article Snippet: .. Simultaneously, fluorescence decay was captured using an inverted Zeiss Axio Observer microscope. ..

    Article Title: Interaction between FmRAV4 (a RAV transcription factor) and FmMYB1 (a MYB transcription factor) confers drought tolerance via ROS scavenging in Fraxinus mandshurica.
    Article Snippet: Drought stress severely restricts plant growth and development.. Although AP2/ERF family transcription factors (TFs) participate extensively in abiotic stress responses, they remain largely unexplored in Fraxinus mandshurica.. In this study, we identified and functionally characterized FmRAV4, a TF from the RAV subfamily.

    Article Title: Phosphorylation of the mitochondrial calcium uniporter beta subunit matrix domain locks a conformation that suppresses mitochondrial calcium flux.
    Article Snippet: .. 827 828 Epifluorescence microscopy 829 Epifluorescence images of live HeLa cells were acquired using a Zeiss AXIO observer D1 inverted 830 microscope equipped with a Sutter Lambda 721 LED optical beam combining system and Zeiss 831 Axiocam 807 mono CCD camera. .. Images were captured through a Zeiss 63×/1.4 Plan-832 Apochromat oil immersion objective, using OBC-480 LED excitation for GCaMP6f and eGFP 833 (200 ms exposure) and OBC-560 LED excitation for MitoTracker Red (150 ms exposure) or 834 mCherry (1 s exposure).

    Article Title: High ex vivo recombination rate of African swine fever virus in porcine alveolar macrophages.
    Article Snippet: .. The cells were monitored and photographed using an Axio Observer D1 inverted fluorescence microscope (Carl Zeiss AG, Oberkochen, Germany). .. Red and green fluorescent signals were detected using Zeiss filter sets 31 Alexa Fluor 568 and 38 HE GFP, respectively.

    Article Title: Enterococcus faecalis biofilm rewires neutrophil metabolism to suppress antimicrobial activity.
    Article Snippet: .. Stained neutrophil samples were imaged using a Zeiss Axio Observer Z1 inverted wide- field microscope at ×20 magnification, unless otherwise specified, using ZEN 2 (blue) software (Zeiss). ..

    Article Title: Early myelin-associated histological and cellular findings following ketogenic diet or ketone ester treatment in a cuprizone/rapamycin mouse model.
    Article Snippet: Background: Impaired restoration of CNS myelin contributes to progressive disability in multiple sclerosis (MS), yet current disease-modifying therapies do not directly target this biology.. Metabolic interventions such as the ketogenic diet (KD) are of interest, but sustained dietary adherence may be limited.. Methods: Male C57BL/6 mice underwent cuprizone/rapamycin demyelination for 6 weeks and, after an additional 7-day rapamycin washout with continued cuprizone exposure, received 9 days of standard diet (SD), ketogenic diet (KD), or intermittent ketone ester injections (iKE) during early recovery.

    Article Title: Sex-dependent transcriptional and imaging responses to metformin in young-adult mice with SuHx-induced pulmonary arterial hypertension
    Article Snippet: Subsequently, the sections were incubated with FITC-conjugated lectin (Sigma, L-4895) diluted in PBS (100 μg/mL) for 90 min to visualise the cell membranes, as WGA specifically binds to membrane glycoproteins. .. Images were captured using an Axio Observer Z1 inverted fluorescence microscope (Zeiss). ..

    Article Title: Oocyte lipid damage from maternal aging marks neurodevelopment in mouse offspring
    Article Snippet: .. 253 Atomic force microscopy 254 Measurements were performed with a BioScope Catalyst atomic force microscope (Bruker: 255 Billerica, MA, USA) coupled with an Axio Observer Z1 inverted optical microscope from Zeiss 256 (Oberkochen, Germany). ..

    Incubation:

    Article Title: Interaction between FmRAV4 (a RAV transcription factor) and FmMYB1 (a MYB transcription factor) confers drought tolerance via ROS scavenging in Fraxinus mandshurica.
    Article Snippet: Drought stress severely restricts plant growth and development.. Although AP2/ERF family transcription factors (TFs) participate extensively in abiotic stress responses, they remain largely unexplored in Fraxinus mandshurica.. In this study, we identified and functionally characterized FmRAV4, a TF from the RAV subfamily.

    Staining:

    Article Title: Interaction between FmRAV4 (a RAV transcription factor) and FmMYB1 (a MYB transcription factor) confers drought tolerance via ROS scavenging in Fraxinus mandshurica.
    Article Snippet: Drought stress severely restricts plant growth and development.. Although AP2/ERF family transcription factors (TFs) participate extensively in abiotic stress responses, they remain largely unexplored in Fraxinus mandshurica.. In this study, we identified and functionally characterized FmRAV4, a TF from the RAV subfamily.

    Article Title: Enterococcus faecalis biofilm rewires neutrophil metabolism to suppress antimicrobial activity.
    Article Snippet: .. Stained neutrophil samples were imaged using a Zeiss Axio Observer Z1 inverted wide- field microscope at ×20 magnification, unless otherwise specified, using ZEN 2 (blue) software (Zeiss). ..

    Epifluorescence Microscopy:

    Article Title: Phosphorylation of the mitochondrial calcium uniporter beta subunit matrix domain locks a conformation that suppresses mitochondrial calcium flux.
    Article Snippet: .. 827 828 Epifluorescence microscopy 829 Epifluorescence images of live HeLa cells were acquired using a Zeiss AXIO observer D1 inverted 830 microscope equipped with a Sutter Lambda 721 LED optical beam combining system and Zeiss 831 Axiocam 807 mono CCD camera. .. Images were captured through a Zeiss 63×/1.4 Plan-832 Apochromat oil immersion objective, using OBC-480 LED excitation for GCaMP6f and eGFP 833 (200 ms exposure) and OBC-560 LED excitation for MitoTracker Red (150 ms exposure) or 834 mCherry (1 s exposure).

    Software:

    Article Title: Enterococcus faecalis biofilm rewires neutrophil metabolism to suppress antimicrobial activity.
    Article Snippet: .. Stained neutrophil samples were imaged using a Zeiss Axio Observer Z1 inverted wide- field microscope at ×20 magnification, unless otherwise specified, using ZEN 2 (blue) software (Zeiss). ..

    Article Title: Early myelin-associated histological and cellular findings following ketogenic diet or ketone ester treatment in a cuprizone/rapamycin mouse model.
    Article Snippet: Background: Impaired restoration of CNS myelin contributes to progressive disability in multiple sclerosis (MS), yet current disease-modifying therapies do not directly target this biology.. Metabolic interventions such as the ketogenic diet (KD) are of interest, but sustained dietary adherence may be limited.. Methods: Male C57BL/6 mice underwent cuprizone/rapamycin demyelination for 6 weeks and, after an additional 7-day rapamycin washout with continued cuprizone exposure, received 9 days of standard diet (SD), ketogenic diet (KD), or intermittent ketone ester injections (iKE) during early recovery.

    Spinning Disk:

    Article Title: Early myelin-associated histological and cellular findings following ketogenic diet or ketone ester treatment in a cuprizone/rapamycin mouse model.
    Article Snippet: Background: Impaired restoration of CNS myelin contributes to progressive disability in multiple sclerosis (MS), yet current disease-modifying therapies do not directly target this biology.. Metabolic interventions such as the ketogenic diet (KD) are of interest, but sustained dietary adherence may be limited.. Methods: Male C57BL/6 mice underwent cuprizone/rapamycin demyelination for 6 weeks and, after an additional 7-day rapamycin washout with continued cuprizone exposure, received 9 days of standard diet (SD), ketogenic diet (KD), or intermittent ketone ester injections (iKE) during early recovery.

    Confocal:

    Article Title: Early myelin-associated histological and cellular findings following ketogenic diet or ketone ester treatment in a cuprizone/rapamycin mouse model.
    Article Snippet: Background: Impaired restoration of CNS myelin contributes to progressive disability in multiple sclerosis (MS), yet current disease-modifying therapies do not directly target this biology.. Metabolic interventions such as the ketogenic diet (KD) are of interest, but sustained dietary adherence may be limited.. Methods: Male C57BL/6 mice underwent cuprizone/rapamycin demyelination for 6 weeks and, after an additional 7-day rapamycin washout with continued cuprizone exposure, received 9 days of standard diet (SD), ketogenic diet (KD), or intermittent ketone ester injections (iKE) during early recovery.

    AFM Microscopy:

    Article Title: Oocyte lipid damage from maternal aging marks neurodevelopment in mouse offspring
    Article Snippet: .. 253 Atomic force microscopy 254 Measurements were performed with a BioScope Catalyst atomic force microscope (Bruker: 255 Billerica, MA, USA) coupled with an Axio Observer Z1 inverted optical microscope from Zeiss 256 (Oberkochen, Germany). ..



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    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging <t>Microscope</t> with transillumination ( Trans ) or <t>fluorescence</t> illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.
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    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging <t>Microscope</t> with transillumination ( Trans ) or <t>fluorescence</t> illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.
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    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging <t>Microscope</t> with transillumination ( Trans ) or <t>fluorescence</t> illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.
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    Image Search Results


    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging Microscope with transillumination ( Trans ) or fluorescence illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.

    Journal: bioRxiv

    Article Title: Membrane-anchored PrP Sc is the trigger for prion synaptotoxicity

    doi: 10.1101/2025.07.11.664221

    Figure Lengend Snippet: (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging Microscope with transillumination ( Trans ) or fluorescence illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.

    Article Snippet: 10-15 neurons were randomly selected and imaged using a Zeiss AxioObserver D1 Fluorescence Microscope and/or a Zeiss LSM 700 Laser Scanning Confocal Microscope with 63X oil objectives.

    Techniques: Control, Expressing, Live Cell Imaging, Microscopy, Fluorescence, Transduction, Staining, Comparison