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wide field fluorescence microscope  (Carl Zeiss)


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    Structured Review

    Carl Zeiss wide field fluorescence microscope
    Wide Field Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 98/100, based on 1043 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axio+observer+7+microscope/Microscope+stand+Axio+Observer+7/pm42882450-68-8-11
    Average 98 stars, based on 1043 article reviews
    wide field fluorescence microscope - by Bioz Stars, 2026-11
    98/100 stars

    Images

    Related Articles

    Imaging:

    Article Title: A USP28-TM9SF3-Golgiphagy axis maintains glycosylation homeostasis and promotes breast cancer progression.
    Article Snippet: The sections were then mounted using a DAPIcontaining anti-fluorescence quenching medium (Beyotime, P0125) and sealed with nail polish. .. Imaging was conducted using a Zeiss Axio Observer 7 microscope. .. Syngeneic 4T1 BALB/c mouse models All animal procedures were approved by the Animal Care and Use Committee of Wuhan University’s Medical Research Institute (Approval No. MRI 2025-LAC121).

    Microscopy:

    Article Title: A USP28-TM9SF3-Golgiphagy axis maintains glycosylation homeostasis and promotes breast cancer progression.
    Article Snippet: The sections were then mounted using a DAPIcontaining anti-fluorescence quenching medium (Beyotime, P0125) and sealed with nail polish. .. Imaging was conducted using a Zeiss Axio Observer 7 microscope. .. Syngeneic 4T1 BALB/c mouse models All animal procedures were approved by the Animal Care and Use Committee of Wuhan University’s Medical Research Institute (Approval No. MRI 2025-LAC121).

    Article Title: The N-Ank protein Ankrd24 shapes neurons and couples to syndapin and the F-actin promoter Cobl-like.
    Article Snippet: .. 5 μg of the liposomes were then incubated with 2 μM of (each) purified fluorescent (fusion) protein in 400 μl cytosol buffer (25 mM HEPES, pH 7.2, 25 mM KCl, 2.5 mM Mg-acetate, and 100 mM K-glutamate), mixed, and transferred into an open coverslip holder placed in a temperature-controlled incubator built around a spinning disc microscope based on a motorized Axio Observer (Zeiss) equipped with a spinning disc unit CSUX1A 5000, with a 488 nm/100 mW OPSL laser, with 545 nm/40 mW diode lasers, as well as with a QuantEM 512SC EMCCD camera (Zeiss). .. The mixtures were imaged at 37°C and low laser powers (12% laser power; exposure times, 40 ms per channel; 63×/1.20W Korr M27 objective; Zeiss), with a frame rate of 1 multiple color Z-stack per 10 s for 10 min.

    Article Title: Distinct Mitochondrial Recovery Dynamics Following Taxol Removal in Triple-Negative Breast Cancer Cells.
    Article Snippet: .. Briefly, live-cell culture dishes were imaged using a wide-field fluorescence microscope (Zeiss Observer 7, White Plains, NY, USA). ..

    Article Title: Structure-Based Design of HDAC6 Inhibitors Targeting the L2 Pocket: Modulating Autophagy and Liquid-Liquid Phase Separation in Lung Cancer.
    Article Snippet: The working solution contained: • Calcein-AM, a live-cell indicator (excitation/emission: 494/517 nm; GFP/FITC filter set); • Ethidium homodimer-1 (EthD-1), a dead-cell indicator (excitation/emission: 528/617 nm; TRITC/RFP or Cy5 filter set). .. Fluorescence images were acquired using a ZEISS Axio Observer 7 microscope equipped with Colibri 5 (Carl Zeiss AG, Oberkochen, Germany). ..

    Article Title: A novel approach for reliable differentiation of lymphatic endothelial precursor cells in vitro.
    Article Snippet: Afterwards, samples were immunostained with anti-mouse PDPN (127402, BioLegend), anti-mouse LYVE1 (103-PA50AG, Reliatech), and anti-TdTomato (orb182397, Byorbyt) as the primary antibodies; and Alexa Fluor 647-conjugated anti-hamster (A21451, Invitrogen), Alexa Fluor 488-conjugated anti-rabbit (A11034, Molecular Probes) and Alexa Fluor 555-conjugated anti-goat (A21432, Molecular Probes) as secondary antibodies (Table 1 3 S1). .. Immunofluorescence images were taken using Zeiss AxioObserver 7 and LSM900 confocal microscope (Zeiss). .. The expression of PDPN, SCA-1, CD90.2, CD105, CD44, CD31 (PECAM1), CD34, CD45 and CD11 of the isolated and differentiated cells was detected using the APCconjugated anti-mouse PDPN (127410, BioLegend), PE-Cy7-conjugated anti-mouse SCA-1 (558162, BioLegend), anti-mouse CD90.2 (553009, BD Biosciences), antimouse CD105 (14-1051-82, ThermoFisher), anti-mouse CD44 (553134, BD Biosciences), PE-conjugated antimouse CD31 (553373, BD Biosciences), PE-conjugated anti-mouse CD34 (551387, BD Biosciences), anti-mouse CD45 (550539, BD Biosciences) and anti-mouse CD11 (M1/70.15.11.5.2, DSHB).

    Liposomes:

    Article Title: The N-Ank protein Ankrd24 shapes neurons and couples to syndapin and the F-actin promoter Cobl-like.
    Article Snippet: .. 5 μg of the liposomes were then incubated with 2 μM of (each) purified fluorescent (fusion) protein in 400 μl cytosol buffer (25 mM HEPES, pH 7.2, 25 mM KCl, 2.5 mM Mg-acetate, and 100 mM K-glutamate), mixed, and transferred into an open coverslip holder placed in a temperature-controlled incubator built around a spinning disc microscope based on a motorized Axio Observer (Zeiss) equipped with a spinning disc unit CSUX1A 5000, with a 488 nm/100 mW OPSL laser, with 545 nm/40 mW diode lasers, as well as with a QuantEM 512SC EMCCD camera (Zeiss). .. The mixtures were imaged at 37°C and low laser powers (12% laser power; exposure times, 40 ms per channel; 63×/1.20W Korr M27 objective; Zeiss), with a frame rate of 1 multiple color Z-stack per 10 s for 10 min.

    Incubation:

    Article Title: The N-Ank protein Ankrd24 shapes neurons and couples to syndapin and the F-actin promoter Cobl-like.
    Article Snippet: .. 5 μg of the liposomes were then incubated with 2 μM of (each) purified fluorescent (fusion) protein in 400 μl cytosol buffer (25 mM HEPES, pH 7.2, 25 mM KCl, 2.5 mM Mg-acetate, and 100 mM K-glutamate), mixed, and transferred into an open coverslip holder placed in a temperature-controlled incubator built around a spinning disc microscope based on a motorized Axio Observer (Zeiss) equipped with a spinning disc unit CSUX1A 5000, with a 488 nm/100 mW OPSL laser, with 545 nm/40 mW diode lasers, as well as with a QuantEM 512SC EMCCD camera (Zeiss). .. The mixtures were imaged at 37°C and low laser powers (12% laser power; exposure times, 40 ms per channel; 63×/1.20W Korr M27 objective; Zeiss), with a frame rate of 1 multiple color Z-stack per 10 s for 10 min.

    Purification:

    Article Title: The N-Ank protein Ankrd24 shapes neurons and couples to syndapin and the F-actin promoter Cobl-like.
    Article Snippet: .. 5 μg of the liposomes were then incubated with 2 μM of (each) purified fluorescent (fusion) protein in 400 μl cytosol buffer (25 mM HEPES, pH 7.2, 25 mM KCl, 2.5 mM Mg-acetate, and 100 mM K-glutamate), mixed, and transferred into an open coverslip holder placed in a temperature-controlled incubator built around a spinning disc microscope based on a motorized Axio Observer (Zeiss) equipped with a spinning disc unit CSUX1A 5000, with a 488 nm/100 mW OPSL laser, with 545 nm/40 mW diode lasers, as well as with a QuantEM 512SC EMCCD camera (Zeiss). .. The mixtures were imaged at 37°C and low laser powers (12% laser power; exposure times, 40 ms per channel; 63×/1.20W Korr M27 objective; Zeiss), with a frame rate of 1 multiple color Z-stack per 10 s for 10 min.

    Incubator:

    Article Title: The N-Ank protein Ankrd24 shapes neurons and couples to syndapin and the F-actin promoter Cobl-like.
    Article Snippet: .. 5 μg of the liposomes were then incubated with 2 μM of (each) purified fluorescent (fusion) protein in 400 μl cytosol buffer (25 mM HEPES, pH 7.2, 25 mM KCl, 2.5 mM Mg-acetate, and 100 mM K-glutamate), mixed, and transferred into an open coverslip holder placed in a temperature-controlled incubator built around a spinning disc microscope based on a motorized Axio Observer (Zeiss) equipped with a spinning disc unit CSUX1A 5000, with a 488 nm/100 mW OPSL laser, with 545 nm/40 mW diode lasers, as well as with a QuantEM 512SC EMCCD camera (Zeiss). .. The mixtures were imaged at 37°C and low laser powers (12% laser power; exposure times, 40 ms per channel; 63×/1.20W Korr M27 objective; Zeiss), with a frame rate of 1 multiple color Z-stack per 10 s for 10 min.

    Staining:

    Article Title: Brown adipose tissue ankyrin-B cell-autonomously modulates lipid content and its deletion protects male mice against diet-induced obesity
    Article Snippet: .. Single plane images of BAT, WAT, and liver sections stained with H&E were acquired with a Zeiss Axio Observer 7 inverted microscope equipped with a 20x objective. ..

    Inverted Microscopy:

    Article Title: Brown adipose tissue ankyrin-B cell-autonomously modulates lipid content and its deletion protects male mice against diet-induced obesity
    Article Snippet: .. Single plane images of BAT, WAT, and liver sections stained with H&E were acquired with a Zeiss Axio Observer 7 inverted microscope equipped with a 20x objective. ..

    Mass Spectrometry Imaging:

    Article Title: Compartment-specific PPARδ activity controls the outcome of cancer-immune cell competition and immunotherapy efficacy.
    Article Snippet: .. MALDI mass spectrometry imaging To perform spatial metabolomics94, AKPS tumors from WT or PPARδ-iGOF mice were dissected at day 21, weighed, snap-frozen in liquid nitrogen, stored in −80 °C, and cryosectioned at 10 μm onto indium tin oxide-coated slides, with serial H&E-stained sections scanned at ×20 on a Zeiss Axio Observer 7. .. For small-molecule metabolite imaging, a 1,5-diaminonaphthalene hydrochloride matrix solution (4.3 mg ml−1 in a 4.5:5:0.5 mixture of HPLC-grade water, ethanol and 1 M hydrochloric acid, vol/vol/vol) was applied to an indium tin oxide-coated slide using a robotic sprayer (HTX Imaging) with the following parameters: four passes, 0.09 ml min−1 flow rate, 1,200 mm min−1 spray velocity, 75 °C spray nozzle temperature, 55 °C heated tray temperature, 10 psi nitrogen gas pressure, and 2-mm track spacing.

    Fluorescence:

    Article Title: Distinct Mitochondrial Recovery Dynamics Following Taxol Removal in Triple-Negative Breast Cancer Cells.
    Article Snippet: .. Briefly, live-cell culture dishes were imaged using a wide-field fluorescence microscope (Zeiss Observer 7, White Plains, NY, USA). ..

    Article Title: Structure-Based Design of HDAC6 Inhibitors Targeting the L2 Pocket: Modulating Autophagy and Liquid-Liquid Phase Separation in Lung Cancer.
    Article Snippet: The working solution contained: • Calcein-AM, a live-cell indicator (excitation/emission: 494/517 nm; GFP/FITC filter set); • Ethidium homodimer-1 (EthD-1), a dead-cell indicator (excitation/emission: 528/617 nm; TRITC/RFP or Cy5 filter set). .. Fluorescence images were acquired using a ZEISS Axio Observer 7 microscope equipped with Colibri 5 (Carl Zeiss AG, Oberkochen, Germany). ..

    Article Title: ROS-responsive HAMA microneedle patch enables localised asiaticoside delivery to promote diabetic wound repair
    Article Snippet: Diabetic wounds remain a clinical challenge because oxidative stress, persistent inflammation, and impaired tissue repair reinforce one another within the wound microenvironment.. Asiaticoside (AC) is a pro-regenerative triterpenoid, but its therapeutic application in chronic wounds is limited by insufficient local retention and poor adaptation to pathological wound conditions.. Here, we developed a phenylboronic acid-modified hyaluronic acid methacryloyl (HAMA-PBA) microneedle (MN) patch for localised, ROS-responsive delivery of AC (ACHP@MN).

    Immunofluorescence:

    Article Title: A novel approach for reliable differentiation of lymphatic endothelial precursor cells in vitro.
    Article Snippet: Afterwards, samples were immunostained with anti-mouse PDPN (127402, BioLegend), anti-mouse LYVE1 (103-PA50AG, Reliatech), and anti-TdTomato (orb182397, Byorbyt) as the primary antibodies; and Alexa Fluor 647-conjugated anti-hamster (A21451, Invitrogen), Alexa Fluor 488-conjugated anti-rabbit (A11034, Molecular Probes) and Alexa Fluor 555-conjugated anti-goat (A21432, Molecular Probes) as secondary antibodies (Table 1 3 S1). .. Immunofluorescence images were taken using Zeiss AxioObserver 7 and LSM900 confocal microscope (Zeiss). .. The expression of PDPN, SCA-1, CD90.2, CD105, CD44, CD31 (PECAM1), CD34, CD45 and CD11 of the isolated and differentiated cells was detected using the APCconjugated anti-mouse PDPN (127410, BioLegend), PE-Cy7-conjugated anti-mouse SCA-1 (558162, BioLegend), anti-mouse CD90.2 (553009, BD Biosciences), antimouse CD105 (14-1051-82, ThermoFisher), anti-mouse CD44 (553134, BD Biosciences), PE-conjugated antimouse CD31 (553373, BD Biosciences), PE-conjugated anti-mouse CD34 (551387, BD Biosciences), anti-mouse CD45 (550539, BD Biosciences) and anti-mouse CD11 (M1/70.15.11.5.2, DSHB).

    Confocal:

    Article Title: A novel approach for reliable differentiation of lymphatic endothelial precursor cells in vitro.
    Article Snippet: Afterwards, samples were immunostained with anti-mouse PDPN (127402, BioLegend), anti-mouse LYVE1 (103-PA50AG, Reliatech), and anti-TdTomato (orb182397, Byorbyt) as the primary antibodies; and Alexa Fluor 647-conjugated anti-hamster (A21451, Invitrogen), Alexa Fluor 488-conjugated anti-rabbit (A11034, Molecular Probes) and Alexa Fluor 555-conjugated anti-goat (A21432, Molecular Probes) as secondary antibodies (Table 1 3 S1). .. Immunofluorescence images were taken using Zeiss AxioObserver 7 and LSM900 confocal microscope (Zeiss). .. The expression of PDPN, SCA-1, CD90.2, CD105, CD44, CD31 (PECAM1), CD34, CD45 and CD11 of the isolated and differentiated cells was detected using the APCconjugated anti-mouse PDPN (127410, BioLegend), PE-Cy7-conjugated anti-mouse SCA-1 (558162, BioLegend), anti-mouse CD90.2 (553009, BD Biosciences), antimouse CD105 (14-1051-82, ThermoFisher), anti-mouse CD44 (553134, BD Biosciences), PE-conjugated antimouse CD31 (553373, BD Biosciences), PE-conjugated anti-mouse CD34 (551387, BD Biosciences), anti-mouse CD45 (550539, BD Biosciences) and anti-mouse CD11 (M1/70.15.11.5.2, DSHB).



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