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zeiss inverted fluorescence microscope  (Carl Zeiss)


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    Structured Review

    Carl Zeiss zeiss inverted fluorescence microscope
    Synthesis and characterization of BA-HPCS@CGRP microspheres based on microfluidic fabrication. A. Fourier transform infrared spectroscopy spectra of the HPCS, 3-Carboxyphenylboronic acid (BA), and BA-HPCS. B. The hydrogel precursors appear as a liquid macroscopically before gelation. C. The hydrogels appear milky white after photo-crosslinking. D. The imaging of BA-HPCS@CGRP microspheres based on microfluidic chips: macroscopic and microscopic observations. E. Particle size distribution of BA-HPCS@CGRP microspheres. F and G. Representative scanning electron <t>microscope</t> images of BA-HPCS@CGRP microspheres. H. The pore size distribution of lyophilized BA-HPCS@CGRP microspheres. I. The releasing of CGRP from BA-HPCS@CGRP in PBS and different glucose conditions (100 mg/dL, 400 mg/dL). J. Representative live/dead <t>fluorescence</t> images of L929 cells after co-culture with microspheres (green calcein-AM for live cells, red propidium iodide for dead cells). K. The quantitative analysis of L929 cell viability co-cultured with microspheres. ns, no significance. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Zeiss Inverted Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 98/100, based on 991 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axio+observer+7+microscope/Inverted+microscope+Axio+Observer+7/pmc13014975-149-14-18
    Average 98 stars, based on 991 article reviews
    zeiss inverted fluorescence microscope - by Bioz Stars, 2026-10
    98/100 stars

    Images

    1) Product Images from "Sustained-release CGRP microspheres accelerate diabetic wound healing by synergistically promoting neurovascular regeneration through modulation of macrophage and endothelial cell functions"

    Article Title: Sustained-release CGRP microspheres accelerate diabetic wound healing by synergistically promoting neurovascular regeneration through modulation of macrophage and endothelial cell functions

    Journal: Materials Today Bio

    doi: 10.1016/j.mtbio.2026.103015

    Synthesis and characterization of BA-HPCS@CGRP microspheres based on microfluidic fabrication. A. Fourier transform infrared spectroscopy spectra of the HPCS, 3-Carboxyphenylboronic acid (BA), and BA-HPCS. B. The hydrogel precursors appear as a liquid macroscopically before gelation. C. The hydrogels appear milky white after photo-crosslinking. D. The imaging of BA-HPCS@CGRP microspheres based on microfluidic chips: macroscopic and microscopic observations. E. Particle size distribution of BA-HPCS@CGRP microspheres. F and G. Representative scanning electron microscope images of BA-HPCS@CGRP microspheres. H. The pore size distribution of lyophilized BA-HPCS@CGRP microspheres. I. The releasing of CGRP from BA-HPCS@CGRP in PBS and different glucose conditions (100 mg/dL, 400 mg/dL). J. Representative live/dead fluorescence images of L929 cells after co-culture with microspheres (green calcein-AM for live cells, red propidium iodide for dead cells). K. The quantitative analysis of L929 cell viability co-cultured with microspheres. ns, no significance. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Synthesis and characterization of BA-HPCS@CGRP microspheres based on microfluidic fabrication. A. Fourier transform infrared spectroscopy spectra of the HPCS, 3-Carboxyphenylboronic acid (BA), and BA-HPCS. B. The hydrogel precursors appear as a liquid macroscopically before gelation. C. The hydrogels appear milky white after photo-crosslinking. D. The imaging of BA-HPCS@CGRP microspheres based on microfluidic chips: macroscopic and microscopic observations. E. Particle size distribution of BA-HPCS@CGRP microspheres. F and G. Representative scanning electron microscope images of BA-HPCS@CGRP microspheres. H. The pore size distribution of lyophilized BA-HPCS@CGRP microspheres. I. The releasing of CGRP from BA-HPCS@CGRP in PBS and different glucose conditions (100 mg/dL, 400 mg/dL). J. Representative live/dead fluorescence images of L929 cells after co-culture with microspheres (green calcein-AM for live cells, red propidium iodide for dead cells). K. The quantitative analysis of L929 cell viability co-cultured with microspheres. ns, no significance. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Fourier Transform Infrared Spectroscopy, Spectroscopy, Imaging, Microscopy, Pore Size, Fluorescence, Co-Culture Assay, Cell Culture

    Related Articles

    Fluorescence:

    Article Title: Domain-autonomous MKLP1 modules divergently regulate mitotic fidelity and polyploidy in a holocentric system
    Article Snippet: .. Images were acquired using a ZEISS Axio Observer 7 inverted fluorescence microscope (Carl Zeiss AG, Oberkochen, Germany) equipped with appropriate filter sets. ..

    Article Title: Engineering Spatially Localized Bioactive Microenvironments in Assembly Implants for Spatially Extended Bone Ingrowth
    Article Snippet: For CCK‐8 analysis, 300 μL of 10% CCK‐8 working solution (Dojindo, Japan) was added to each sample and incubated at 37°C for 2 h. Subsequently, 100 μL of the reaction solution was transferred to a 96‐well plate, and absorbance was measured at 450 nm. .. For live/dead staining, samples were incubated with 1 mL of Calcein‐AM/PI staining solution (Beyotime, China) at 37°C in the dark for 30 min, washed with PBS, and imaged using an inverted fluorescence microscope (Axio Observer 7, Zeiss, Germany). ..

    Article Title: TiFM2.0 – versatile mechanical measurement and actuation in live embryos
    Article Snippet: Voltage controller , Sending driving programmes to the electrical piezo, voltages are related to X C although offsets and an error margin exist , Thorlabs , MDT693B , x. .. Base microscope , Inverted widefield and fluorescence. Axio Observer 7, 2.5/5/10/20× objectives, xy motorised stage, side camera port, fluorescence filters and LED light source for RL imaging, objective holder z -axis recording, associated controllers , Visualisation of the probes (X T ) and the sample. Different magnifications are essential for adjusting probe location followed by fine alignment. Z -axis recording is essential for determining probe insertion depth. Top illumination and condenser removed to allow installation of the probe head , Zeiss , https://www.micro-shop.zeiss.com/en/uk/system/inverted+microscopes-axio+observer-microscopes/6001/ , x (any inverted scope). ..

    Article Title: Hesperidin-loaded Eudragit S100 nanoparticles alleviate ulcerative colitis by repairing intestinal barrier and modulating gut microbiota
    Article Snippet: .. Finally, the cells were stained with the Calcein-AM/PI assay kit (Beyotime, Shanghai, China) and observed under an inverted fluorescence microscope (Zeiss Axio Observer7, Germany). ..

    Microscopy:

    Article Title: Domain-autonomous MKLP1 modules divergently regulate mitotic fidelity and polyploidy in a holocentric system
    Article Snippet: .. Images were acquired using a ZEISS Axio Observer 7 inverted fluorescence microscope (Carl Zeiss AG, Oberkochen, Germany) equipped with appropriate filter sets. ..

    Article Title: Engineering Spatially Localized Bioactive Microenvironments in Assembly Implants for Spatially Extended Bone Ingrowth
    Article Snippet: For CCK‐8 analysis, 300 μL of 10% CCK‐8 working solution (Dojindo, Japan) was added to each sample and incubated at 37°C for 2 h. Subsequently, 100 μL of the reaction solution was transferred to a 96‐well plate, and absorbance was measured at 450 nm. .. For live/dead staining, samples were incubated with 1 mL of Calcein‐AM/PI staining solution (Beyotime, China) at 37°C in the dark for 30 min, washed with PBS, and imaged using an inverted fluorescence microscope (Axio Observer 7, Zeiss, Germany). ..

    Article Title: MED12 mutation activates Notch signaling to enhance cancer stemness and suppress CD8+ T cell cytotoxicity in lung cancer
    Article Snippet: .. On day 14, the formed spheres were photographed under an inverted Zeiss Axio Observer 7 microscope (Zeiss, Germany), and the number of spheres (diameter > 50 μm) was counted in five random fields per well. ..

    Article Title: TiFM2.0 – versatile mechanical measurement and actuation in live embryos
    Article Snippet: Voltage controller , Sending driving programmes to the electrical piezo, voltages are related to X C although offsets and an error margin exist , Thorlabs , MDT693B , x. .. Base microscope , Inverted widefield and fluorescence. Axio Observer 7, 2.5/5/10/20× objectives, xy motorised stage, side camera port, fluorescence filters and LED light source for RL imaging, objective holder z -axis recording, associated controllers , Visualisation of the probes (X T ) and the sample. Different magnifications are essential for adjusting probe location followed by fine alignment. Z -axis recording is essential for determining probe insertion depth. Top illumination and condenser removed to allow installation of the probe head , Zeiss , https://www.micro-shop.zeiss.com/en/uk/system/inverted+microscopes-axio+observer-microscopes/6001/ , x (any inverted scope). ..

    Article Title: Hesperidin-loaded Eudragit S100 nanoparticles alleviate ulcerative colitis by repairing intestinal barrier and modulating gut microbiota
    Article Snippet: .. Finally, the cells were stained with the Calcein-AM/PI assay kit (Beyotime, Shanghai, China) and observed under an inverted fluorescence microscope (Zeiss Axio Observer7, Germany). ..

    Staining:

    Article Title: Engineering Spatially Localized Bioactive Microenvironments in Assembly Implants for Spatially Extended Bone Ingrowth
    Article Snippet: For CCK‐8 analysis, 300 μL of 10% CCK‐8 working solution (Dojindo, Japan) was added to each sample and incubated at 37°C for 2 h. Subsequently, 100 μL of the reaction solution was transferred to a 96‐well plate, and absorbance was measured at 450 nm. .. For live/dead staining, samples were incubated with 1 mL of Calcein‐AM/PI staining solution (Beyotime, China) at 37°C in the dark for 30 min, washed with PBS, and imaged using an inverted fluorescence microscope (Axio Observer 7, Zeiss, Germany). ..

    Article Title: Hesperidin-loaded Eudragit S100 nanoparticles alleviate ulcerative colitis by repairing intestinal barrier and modulating gut microbiota
    Article Snippet: .. Finally, the cells were stained with the Calcein-AM/PI assay kit (Beyotime, Shanghai, China) and observed under an inverted fluorescence microscope (Zeiss Axio Observer7, Germany). ..

    Incubation:

    Article Title: Engineering Spatially Localized Bioactive Microenvironments in Assembly Implants for Spatially Extended Bone Ingrowth
    Article Snippet: For CCK‐8 analysis, 300 μL of 10% CCK‐8 working solution (Dojindo, Japan) was added to each sample and incubated at 37°C for 2 h. Subsequently, 100 μL of the reaction solution was transferred to a 96‐well plate, and absorbance was measured at 450 nm. .. For live/dead staining, samples were incubated with 1 mL of Calcein‐AM/PI staining solution (Beyotime, China) at 37°C in the dark for 30 min, washed with PBS, and imaged using an inverted fluorescence microscope (Axio Observer 7, Zeiss, Germany). ..

    Inverted Microscopy:

    Article Title: Stanniocalcin 1 contributes to glioma stem cells phenotypic plasticity through NOTCH1/STAT3/GFPT2 axis.
    Article Snippet: .. After 7 to 10 days, neurospheres with diameter over 50 μm were photographed and counted using an inverted microscope (Axio Observer 7, ZEISS, Germany), and then measured the diameter with Image J software (National Institutes of Health, USA). .. GSCs were pretreated with FBS-free DMEM/F12 and seeded onto 8-μm pole filters (BD Falcon, USA) and matrigel (Corning, USA)-coated filters, respectively.

    Article Title: Protocol to study murine ovarian elasticity and composition in situ by integrating quantitative micro-elastography with light microscopy
    Article Snippet: .. The equipment and settings for the presented protocol used were as follows: a. Zeiss Axio Observer.Z1/7 inverted microscope with LSM 980 confocal scanner. b. ..

    Article Title: Impact of hypofractionated radiotherapy on 3D breast cancer models
    Article Snippet: The staining solution was made using a 1: 1: 998 ratio of FDA: PI: PBS (phosphate buffered saline, Thermo Fisher Scientific) (stock solutions: FDA = 10 mg/mL, PI = 5 mg/mL, PBS. .. Cells in GelMA hydrogels was loaded onto the ZEISS Inverted microscope Axio Observer 7 (Carl Zeiss, United States). ..

    Software:

    Article Title: Stanniocalcin 1 contributes to glioma stem cells phenotypic plasticity through NOTCH1/STAT3/GFPT2 axis.
    Article Snippet: .. After 7 to 10 days, neurospheres with diameter over 50 μm were photographed and counted using an inverted microscope (Axio Observer 7, ZEISS, Germany), and then measured the diameter with Image J software (National Institutes of Health, USA). .. GSCs were pretreated with FBS-free DMEM/F12 and seeded onto 8-μm pole filters (BD Falcon, USA) and matrigel (Corning, USA)-coated filters, respectively.

    Imaging:

    Article Title: TiFM2.0 – versatile mechanical measurement and actuation in live embryos
    Article Snippet: Voltage controller , Sending driving programmes to the electrical piezo, voltages are related to X C although offsets and an error margin exist , Thorlabs , MDT693B , x. .. Base microscope , Inverted widefield and fluorescence. Axio Observer 7, 2.5/5/10/20× objectives, xy motorised stage, side camera port, fluorescence filters and LED light source for RL imaging, objective holder z -axis recording, associated controllers , Visualisation of the probes (X T ) and the sample. Different magnifications are essential for adjusting probe location followed by fine alignment. Z -axis recording is essential for determining probe insertion depth. Top illumination and condenser removed to allow installation of the probe head , Zeiss , https://www.micro-shop.zeiss.com/en/uk/system/inverted+microscopes-axio+observer-microscopes/6001/ , x (any inverted scope). ..



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