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inverted fluorescence microscope  (Carl Zeiss)


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    Structured Review

    Carl Zeiss inverted fluorescence microscope
    Inverted Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 544 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axio+observer+5+microscope/Inverted+microscope+Axio+Observer+5+materials/pmc11772299-61-8-14
    Average 96 stars, based on 544 article reviews
    inverted fluorescence microscope - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Inverted Microscopy:

    Article Title: Constitutive nitric oxide synthases deficiency impairs cyclobutane pyrimidine dimer repair following solar UV exposure in cells and mice.
    Article Snippet: After washing with 1X PBS, sections were incubated with anti- mouse IgG (H + L, 1:200, Vector laboratories) plus DAPI (4′,6- diamidino- 2- phenylindole) (1:2000, Invitrogen) in 1X PBS for 1 h at room temperature and then mounted with mounting media (ProLong Gold antifade reagent, Invitrogen). .. Pictures were taken using the inverted microscope Zeiss Axio Observer 5. ..

    Article Title: Multiple rotavirus species encode fusion-associated small transmembrane (FAST) proteins with cell type-specific activity
    Article Snippet: Monoclonal mouse anti-FLAG antibody (Sigma), Alexa Fluor 488-conjugated anti-mouse IgG (Invitrogen), and rhodamine phalloidin (Invitrogen) are commercially available. .. For differential interference contrast imaging, 293T cells (∼2 × 10 5 per well) in 24-well plates were transfected with 0.1 μg of plasmid DNA per well using LyoVec transfection reagent (InvivoGen), according to manufacturer instructions, incubated for 18 h at 37°C, fixed with 4% paraformaldehyde in PBS, and imaged using a Zeiss Axiovert 200 inverted microscope equipped with an HBO 100 mercury arc lamp or a Nikon STORM with A Hamamatsu ORCA Flash 4.0 CMOS monochrome camera and a Nikon DS-Ri2 color camera. .. For transfections with plasmids expressing untagged NSP1-1 proteins, F-actin was detected with rhodamine phalloidin, and nuclei were detected using 300 nM 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen), with washes in PBS.

    Article Title: A skin explant model for studying UV-induced DNA damage and repair.
    Article Snippet: .. Pictures were taken using the inverted microscope Zeiss Axio Observer 5. sUV irradiation of tissue explants The skin was irradiated with a single dose of sUV, using 180 mJ/cm2 of UVB from a UVA/UVB solar lamp (UVA340 lamp, Q- Lab Corporation) previously characterized.12 After irradiation, the samples were incubated (37°C and 5% CO2) for the specified periods of time. ..

    Imaging:

    Article Title: Multiple rotavirus species encode fusion-associated small transmembrane (FAST) proteins with cell type-specific activity
    Article Snippet: Monoclonal mouse anti-FLAG antibody (Sigma), Alexa Fluor 488-conjugated anti-mouse IgG (Invitrogen), and rhodamine phalloidin (Invitrogen) are commercially available. .. For differential interference contrast imaging, 293T cells (∼2 × 10 5 per well) in 24-well plates were transfected with 0.1 μg of plasmid DNA per well using LyoVec transfection reagent (InvivoGen), according to manufacturer instructions, incubated for 18 h at 37°C, fixed with 4% paraformaldehyde in PBS, and imaged using a Zeiss Axiovert 200 inverted microscope equipped with an HBO 100 mercury arc lamp or a Nikon STORM with A Hamamatsu ORCA Flash 4.0 CMOS monochrome camera and a Nikon DS-Ri2 color camera. .. For transfections with plasmids expressing untagged NSP1-1 proteins, F-actin was detected with rhodamine phalloidin, and nuclei were detected using 300 nM 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen), with washes in PBS.

    Article Title: Exploring the Micro-Mosaic Landscape of FGFR3 Mutations in the Ageing Male Germline and Their Potential Implications in Meiotic Differentiation.
    Article Snippet: .. The array is scanned with an inverted fluorescence microscope (Zeiss Axio Observer.Z1, Munich, Germany) with a 20× objective followed by a subsequent washing, probing, and imaging cycle to confirm the mutants with a dye switch. ..

    Transfection:

    Article Title: Multiple rotavirus species encode fusion-associated small transmembrane (FAST) proteins with cell type-specific activity
    Article Snippet: Monoclonal mouse anti-FLAG antibody (Sigma), Alexa Fluor 488-conjugated anti-mouse IgG (Invitrogen), and rhodamine phalloidin (Invitrogen) are commercially available. .. For differential interference contrast imaging, 293T cells (∼2 × 10 5 per well) in 24-well plates were transfected with 0.1 μg of plasmid DNA per well using LyoVec transfection reagent (InvivoGen), according to manufacturer instructions, incubated for 18 h at 37°C, fixed with 4% paraformaldehyde in PBS, and imaged using a Zeiss Axiovert 200 inverted microscope equipped with an HBO 100 mercury arc lamp or a Nikon STORM with A Hamamatsu ORCA Flash 4.0 CMOS monochrome camera and a Nikon DS-Ri2 color camera. .. For transfections with plasmids expressing untagged NSP1-1 proteins, F-actin was detected with rhodamine phalloidin, and nuclei were detected using 300 nM 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen), with washes in PBS.

    Plasmid Preparation:

    Article Title: Multiple rotavirus species encode fusion-associated small transmembrane (FAST) proteins with cell type-specific activity
    Article Snippet: Monoclonal mouse anti-FLAG antibody (Sigma), Alexa Fluor 488-conjugated anti-mouse IgG (Invitrogen), and rhodamine phalloidin (Invitrogen) are commercially available. .. For differential interference contrast imaging, 293T cells (∼2 × 10 5 per well) in 24-well plates were transfected with 0.1 μg of plasmid DNA per well using LyoVec transfection reagent (InvivoGen), according to manufacturer instructions, incubated for 18 h at 37°C, fixed with 4% paraformaldehyde in PBS, and imaged using a Zeiss Axiovert 200 inverted microscope equipped with an HBO 100 mercury arc lamp or a Nikon STORM with A Hamamatsu ORCA Flash 4.0 CMOS monochrome camera and a Nikon DS-Ri2 color camera. .. For transfections with plasmids expressing untagged NSP1-1 proteins, F-actin was detected with rhodamine phalloidin, and nuclei were detected using 300 nM 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen), with washes in PBS.

    Incubation:

    Article Title: Multiple rotavirus species encode fusion-associated small transmembrane (FAST) proteins with cell type-specific activity
    Article Snippet: Monoclonal mouse anti-FLAG antibody (Sigma), Alexa Fluor 488-conjugated anti-mouse IgG (Invitrogen), and rhodamine phalloidin (Invitrogen) are commercially available. .. For differential interference contrast imaging, 293T cells (∼2 × 10 5 per well) in 24-well plates were transfected with 0.1 μg of plasmid DNA per well using LyoVec transfection reagent (InvivoGen), according to manufacturer instructions, incubated for 18 h at 37°C, fixed with 4% paraformaldehyde in PBS, and imaged using a Zeiss Axiovert 200 inverted microscope equipped with an HBO 100 mercury arc lamp or a Nikon STORM with A Hamamatsu ORCA Flash 4.0 CMOS monochrome camera and a Nikon DS-Ri2 color camera. .. For transfections with plasmids expressing untagged NSP1-1 proteins, F-actin was detected with rhodamine phalloidin, and nuclei were detected using 300 nM 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen), with washes in PBS.

    Article Title: A skin explant model for studying UV-induced DNA damage and repair.
    Article Snippet: .. Pictures were taken using the inverted microscope Zeiss Axio Observer 5. sUV irradiation of tissue explants The skin was irradiated with a single dose of sUV, using 180 mJ/cm2 of UVB from a UVA/UVB solar lamp (UVA340 lamp, Q- Lab Corporation) previously characterized.12 After irradiation, the samples were incubated (37°C and 5% CO2) for the specified periods of time. ..

    Microscopy:

    Article Title: Glucagon-like peptide-2 pharmacotherapy activates hepatic Farnesoid X receptor-signaling to attenuate resection-associated bile acid loss in mice.
    Article Snippet: .. For quantification of villus height, 5 μm thick 230 tissue slides were made, hematoxylin and eosin-stained and five well-oriented, full-length 231 crypts and villi per sample were measured and averaged using an Axio Observer inverted 232 microscope (Zeiss) and Zen 2.3 software. .. To evaluate proliferative activity, ki-67 was 233 immunohistochemically stained using ImmPress polymer reaction (Vector Laboratories) with 234 the Ki-67 Monoclonal Antibody SolA15, eBioscienceTM, catalogue-no.: 14-5698-80.

    Article Title: A blocking ELISA based on virus-like nanoparticles chimerized with an antigenic epitope of ASFV P54 for detecting ASFV antibodies
    Article Snippet: Following three washes with PBS, the cells were incubated with rabbit Anti-Mouse IgG H&L (FITC) (Abcam, CAT: ab6724) for 30 min at 37 °C. .. After being washed thrice with PBS, the cells were observed with an inverted fluorescence microscope (ZEISS Axio Observer Z1). ..

    Article Title: Ultra-small curcumin-ruthenium coordination polymer nanodots prevent renal ischemia-reperfusion injury and the progression to chronic kidney disease
    Article Snippet: .. The internalization of the compounds was observed using inverted fluorescence microscope (Axio Observer 5, ZEISS). ..

    Article Title: miR-340-3p-modified bone marrow mesenchymal stem cell-derived exosomes inhibit ferroptosis through METTL3-mediated m 6 A modification of HMOX1 to promote recovery of injured rat uterus
    Article Snippet: DAPI (Sigma-Aldrich; MBD0015) was used for nuclear counterstaining. .. An inverted fluorescence microscope (Axio Observer, Zeiss) was utilized to acquire the images. ..

    Article Title: Exploring the Micro-Mosaic Landscape of FGFR3 Mutations in the Ageing Male Germline and Their Potential Implications in Meiotic Differentiation.
    Article Snippet: .. The array is scanned with an inverted fluorescence microscope (Zeiss Axio Observer.Z1, Munich, Germany) with a 20× objective followed by a subsequent washing, probing, and imaging cycle to confirm the mutants with a dye switch. ..

    Software:

    Article Title: Glucagon-like peptide-2 pharmacotherapy activates hepatic Farnesoid X receptor-signaling to attenuate resection-associated bile acid loss in mice.
    Article Snippet: .. For quantification of villus height, 5 μm thick 230 tissue slides were made, hematoxylin and eosin-stained and five well-oriented, full-length 231 crypts and villi per sample were measured and averaged using an Axio Observer inverted 232 microscope (Zeiss) and Zen 2.3 software. .. To evaluate proliferative activity, ki-67 was 233 immunohistochemically stained using ImmPress polymer reaction (Vector Laboratories) with 234 the Ki-67 Monoclonal Antibody SolA15, eBioscienceTM, catalogue-no.: 14-5698-80.

    Fluorescence:

    Article Title: A blocking ELISA based on virus-like nanoparticles chimerized with an antigenic epitope of ASFV P54 for detecting ASFV antibodies
    Article Snippet: Following three washes with PBS, the cells were incubated with rabbit Anti-Mouse IgG H&L (FITC) (Abcam, CAT: ab6724) for 30 min at 37 °C. .. After being washed thrice with PBS, the cells were observed with an inverted fluorescence microscope (ZEISS Axio Observer Z1). ..

    Article Title: Ultra-small curcumin-ruthenium coordination polymer nanodots prevent renal ischemia-reperfusion injury and the progression to chronic kidney disease
    Article Snippet: .. The internalization of the compounds was observed using inverted fluorescence microscope (Axio Observer 5, ZEISS). ..

    Article Title: miR-340-3p-modified bone marrow mesenchymal stem cell-derived exosomes inhibit ferroptosis through METTL3-mediated m 6 A modification of HMOX1 to promote recovery of injured rat uterus
    Article Snippet: DAPI (Sigma-Aldrich; MBD0015) was used for nuclear counterstaining. .. An inverted fluorescence microscope (Axio Observer, Zeiss) was utilized to acquire the images. ..

    Article Title: Exploring the Micro-Mosaic Landscape of FGFR3 Mutations in the Ageing Male Germline and Their Potential Implications in Meiotic Differentiation.
    Article Snippet: .. The array is scanned with an inverted fluorescence microscope (Zeiss Axio Observer.Z1, Munich, Germany) with a 20× objective followed by a subsequent washing, probing, and imaging cycle to confirm the mutants with a dye switch. ..

    Irradiation:

    Article Title: A skin explant model for studying UV-induced DNA damage and repair.
    Article Snippet: .. Pictures were taken using the inverted microscope Zeiss Axio Observer 5. sUV irradiation of tissue explants The skin was irradiated with a single dose of sUV, using 180 mJ/cm2 of UVB from a UVA/UVB solar lamp (UVA340 lamp, Q- Lab Corporation) previously characterized.12 After irradiation, the samples were incubated (37°C and 5% CO2) for the specified periods of time. ..



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