rnaeasy plant mini kit  (Qiagen)

Journal: BMC Plant Biology

Article Title: AMS-dependent and independent regulation of anther transcriptome and comparison with those affected by other Arabidopsis anther genes

doi: 10.1186/1471-2229-12-23

Figure Lengend Snippet: A pie graph of GO categorization of genes differentially expressed in the ams mutant . (A-B) GO categorization of genes up- and down-regulated in ams , with enriched categories circled compared with all genes on ATH1. (C) GO categorization of all genes on the ATH1 chip.

Article Snippet: For the identification of the functions of the differentially expressed genes, the annotations of genes on ATH1 microarray chip were downloaded from Affymetrix website and we used the GO categorization function on TAIR website [ ].

Techniques: Mutagenesis

Journal: BMC Plant Biology

Article Title: AMS-dependent and independent regulation of anther transcriptome and comparison with those affected by other Arabidopsis anther genes

doi: 10.1186/1471-2229-12-23

Figure Lengend Snippet: Venn diagrams of microarray results and previous related study . (A) A comparison of anther preferential genes identified in our study with previously known pollen genes and stamen genes. (B-D) Comparisons between genes differentially expressed in the ams anther and those preferentially expressed in certain organ: anther preferential, stamen and pollen respectively.

Article Snippet: For the identification of the functions of the differentially expressed genes, the annotations of genes on ATH1 microarray chip were downloaded from Affymetrix website and we used the GO categorization function on TAIR website [ ].

Techniques: Microarray

Journal: BMC Plant Biology

Article Title: AMS-dependent and independent regulation of anther transcriptome and comparison with those affected by other Arabidopsis anther genes

doi: 10.1186/1471-2229-12-23

Figure Lengend Snippet: Gene regulatory network of anther development during early stages . Gene regulation is represented by T-bars (negatively) and arrows (positively). The direct regulation confirmed by experiment is represented in bold line. Genes encoding proteins with interaction is represented by double arrows. Gene expression patterns in different tissues are shown by colors (blue for anther specific; red for anther-preferential; green for reproductive-preferential and yellow for genes not included in ATH1 chip). Gene function in tapetum formation is marked by an apostrophe; in pollen wall formation by an asterisk; in callose dissolution by double asterisks; in stamen and petal formation by the letter b; in stamen and carpel formation by the letter c.

Article Snippet: For the identification of the functions of the differentially expressed genes, the annotations of genes on ATH1 microarray chip were downloaded from Affymetrix website and we used the GO categorization function on TAIR website [ ].

Techniques: Expressing

Journal: Philosophical Transactions of the Royal Society B: Biological Sciences

Article Title: Joint inhibition of mitochondrial complex IV and alternative oxidase by genetic or chemical means represses chloroplast transcription in Arabidopsis

doi: 10.1098/rstb.2019.0409

Figure Lengend Snippet: Expression level of nuclear genes encoding components of the chloroplast transcription machinery in selected mitochondrial mutants and plants growing under hypoxic conditions. (a) Colour scale represents up (red) or down (blue) fold change values relative to the wild-type. Publicly available dataset GSE 14420 [45] representing three-week-old wild-type plant exposed to 4 h of hypoxia was analysed. (b) Common differentially expressed nuclear genes encoding chloroplast transcription machinery components (ppde. p > 0.95, FC (fold change) 1.5) in representative microarray datasets. (Online version in colour.)

Article Snippet: Analysis of the global changes in transcript abundance in rps10 mutant was performed using Affymetrix ATH1 microarray gene chips.

Techniques: Expressing, Microarray