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lentivirus vector  (ATCC)


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    Structured Review

    ATCC lentivirus vector
    Lentivirus Vector, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atcc+reference+material/Lentivirus+vector%3Breference+material/pmc12283003-303-7-21
    Average 93 stars, based on 10 article reviews
    lentivirus vector - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: KSHV episomes reveal dynamic chromatin loop formation with domain-specific gene regulation.
    Article Snippet: .. The lentivirus vector was produced by tripartite-transfection of pCMV-VSV-G-RSV-Rev, pCAG-HIVgp and pCW57.1KSHVK-Rta/ORF50 into 293 T cells (ATCC CRL-3216)59,60. ..

    Article Title: Microplastics exacerbate ferroptosis via mitochondrial reactive oxygen species-mediated autophagy in chronic obstructive pulmonary disease.
    Article Snippet: .. Briefly, the plasmid was cloned into the lentivirus vector, and then the recombinant lentivirus was further transfected into the HEK-293T cells (ATCC, CRL-3216) for amplification and concentration. ..

    Article Title: Treatment of cancer using chimeric CD3 receptor proteins
    Article Snippet: .. Lentivirus vector titers were determined using limited dilution on Sup-T1 cells (ATCC) cultured in RPMI-1640 supplemented with 10% FBS. .. Vectors were 3-fold serial diluted then 50 uL of diluted vector was added to a flat bottom microtiter plate containing Sup-T1 cells.

    Article Title: KSHV episomes reveal dynamic chromatin loop formation with domain-specific gene regulation
    Article Snippet: .. The lentivirus vector was produced by tripartite-transfection of pCMV-VSV-G-RSV-Rev, pCAG-HIVgp and pCW57.1-KSHVK-Rta/ORF50 into 293 T cells (ATCC CRL-3216) , . ..

    Article Title: Erythropoietin-Modified Mesenchymal Stem Cells Enhance Anti-fibrosis Efficacy in Mouse Liver Fibrosis Model
    Article Snippet: .. After the lentivirus vector was constructed, viral packaging was carried out in HEK 293T (ATCC, ACS-4500) cells. ..

    Article Title: Microplastics exacerbate ferroptosis via mitochondrial reactive oxygen species-mediated autophagy in chronic obstructive pulmonary disease
    Article Snippet: .. Briefly, the plasmid was cloned into the lentivirus vector, and then the recombinant lentivirus was further transfected into the HEK-293T cells (ATCC, CRL-3216) for amplification and concentration. ..

    Produced:

    Article Title: KSHV episomes reveal dynamic chromatin loop formation with domain-specific gene regulation.
    Article Snippet: .. The lentivirus vector was produced by tripartite-transfection of pCMV-VSV-G-RSV-Rev, pCAG-HIVgp and pCW57.1KSHVK-Rta/ORF50 into 293 T cells (ATCC CRL-3216)59,60. ..

    Article Title: KSHV episomes reveal dynamic chromatin loop formation with domain-specific gene regulation
    Article Snippet: .. The lentivirus vector was produced by tripartite-transfection of pCMV-VSV-G-RSV-Rev, pCAG-HIVgp and pCW57.1-KSHVK-Rta/ORF50 into 293 T cells (ATCC CRL-3216) , . ..

    Clone Assay:

    Article Title: Microplastics exacerbate ferroptosis via mitochondrial reactive oxygen species-mediated autophagy in chronic obstructive pulmonary disease.
    Article Snippet: .. Briefly, the plasmid was cloned into the lentivirus vector, and then the recombinant lentivirus was further transfected into the HEK-293T cells (ATCC, CRL-3216) for amplification and concentration. ..

    Article Title: Targeting Androgen Receptor to Suppress Macrophage-induced EMT and Benign Prostatic Hyperplasia (BPH) Development
    Article Snippet: .. To generate AR overexpressed stable clones of the BPH-1 cell line, the lentivirus vector, pWPI-AR, or pWPI-control was cotransfected with pMD2.G and pAX2 into 293T cells (ATCC). .. After transfection for 48 h, the cultured medium of 293T cells was harvested and mixed with fresh F-12K culture medium (ratio 1:1) and 8 μg/ml polybrene (Millipore Corp., Billerica, MA), and then incubated with BPH-1 cells for 24 h. The viral-infected BPH-1 cells contain green fluorescence protein (GFP), so the BPH-AR or BPH-vector stable cells were obtained by the flow cytometry sorting.

    Article Title: Targeting Androgen Receptor to Suppress Macrophage-induced EMT and Benign Prostatic Hyperplasia (BPH) Development
    Article Snippet: .. Plasmids and stable cell lines To generate AR overexpressed stable clones of the BPH-1 cell line, the lentivirus vector, pWPI-AR, or pWPI-control was cotransfected with pMD2.G and pAX2 into 293T cells (ATCC). .. After transfection for 48 h, the cultured medium of 293T cells was harvested and mixed with fresh F-12K culture medium (ratio 1:1) and 8 μg/ml polybrene (Millipore Corp., Billerica, MA), and then incubated with BPH-1 cells for 24 h. The viral-infected BPH-1 cells contain green fluorescence protein (GFP), so the BPH-AR or BPH-vector stable cells were obtained by the flow cytometry sorting.

    Article Title: Microplastics exacerbate ferroptosis via mitochondrial reactive oxygen species-mediated autophagy in chronic obstructive pulmonary disease
    Article Snippet: .. Briefly, the plasmid was cloned into the lentivirus vector, and then the recombinant lentivirus was further transfected into the HEK-293T cells (ATCC, CRL-3216) for amplification and concentration. ..

    Recombinant:

    Article Title: Microplastics exacerbate ferroptosis via mitochondrial reactive oxygen species-mediated autophagy in chronic obstructive pulmonary disease.
    Article Snippet: .. Briefly, the plasmid was cloned into the lentivirus vector, and then the recombinant lentivirus was further transfected into the HEK-293T cells (ATCC, CRL-3216) for amplification and concentration. ..

    Article Title: Microplastics exacerbate ferroptosis via mitochondrial reactive oxygen species-mediated autophagy in chronic obstructive pulmonary disease
    Article Snippet: .. Briefly, the plasmid was cloned into the lentivirus vector, and then the recombinant lentivirus was further transfected into the HEK-293T cells (ATCC, CRL-3216) for amplification and concentration. ..

    Transfection:

    Article Title: Microplastics exacerbate ferroptosis via mitochondrial reactive oxygen species-mediated autophagy in chronic obstructive pulmonary disease.
    Article Snippet: .. Briefly, the plasmid was cloned into the lentivirus vector, and then the recombinant lentivirus was further transfected into the HEK-293T cells (ATCC, CRL-3216) for amplification and concentration. ..

    Article Title: Microplastics exacerbate ferroptosis via mitochondrial reactive oxygen species-mediated autophagy in chronic obstructive pulmonary disease
    Article Snippet: .. Briefly, the plasmid was cloned into the lentivirus vector, and then the recombinant lentivirus was further transfected into the HEK-293T cells (ATCC, CRL-3216) for amplification and concentration. ..

    Amplification:

    Article Title: Microplastics exacerbate ferroptosis via mitochondrial reactive oxygen species-mediated autophagy in chronic obstructive pulmonary disease.
    Article Snippet: .. Briefly, the plasmid was cloned into the lentivirus vector, and then the recombinant lentivirus was further transfected into the HEK-293T cells (ATCC, CRL-3216) for amplification and concentration. ..

    Article Title: Microplastics exacerbate ferroptosis via mitochondrial reactive oxygen species-mediated autophagy in chronic obstructive pulmonary disease
    Article Snippet: .. Briefly, the plasmid was cloned into the lentivirus vector, and then the recombinant lentivirus was further transfected into the HEK-293T cells (ATCC, CRL-3216) for amplification and concentration. ..

    Concentration Assay:

    Article Title: Microplastics exacerbate ferroptosis via mitochondrial reactive oxygen species-mediated autophagy in chronic obstructive pulmonary disease.
    Article Snippet: .. Briefly, the plasmid was cloned into the lentivirus vector, and then the recombinant lentivirus was further transfected into the HEK-293T cells (ATCC, CRL-3216) for amplification and concentration. ..

    Article Title: Microplastics exacerbate ferroptosis via mitochondrial reactive oxygen species-mediated autophagy in chronic obstructive pulmonary disease
    Article Snippet: .. Briefly, the plasmid was cloned into the lentivirus vector, and then the recombinant lentivirus was further transfected into the HEK-293T cells (ATCC, CRL-3216) for amplification and concentration. ..

    Cell Culture:

    Article Title: Treatment of cancer using chimeric CD3 receptor proteins
    Article Snippet: .. Lentivirus vector titers were determined using limited dilution on Sup-T1 cells (ATCC) cultured in RPMI-1640 supplemented with 10% FBS. .. Vectors were 3-fold serial diluted then 50 uL of diluted vector was added to a flat bottom microtiter plate containing Sup-T1 cells.

    Construct:

    Article Title: Erythropoietin-Modified Mesenchymal Stem Cells Enhance Anti-fibrosis Efficacy in Mouse Liver Fibrosis Model
    Article Snippet: .. After the lentivirus vector was constructed, viral packaging was carried out in HEK 293T (ATCC, ACS-4500) cells. ..

    Stable Transfection:

    Article Title: Targeting Androgen Receptor to Suppress Macrophage-induced EMT and Benign Prostatic Hyperplasia (BPH) Development
    Article Snippet: .. Plasmids and stable cell lines To generate AR overexpressed stable clones of the BPH-1 cell line, the lentivirus vector, pWPI-AR, or pWPI-control was cotransfected with pMD2.G and pAX2 into 293T cells (ATCC). .. After transfection for 48 h, the cultured medium of 293T cells was harvested and mixed with fresh F-12K culture medium (ratio 1:1) and 8 μg/ml polybrene (Millipore Corp., Billerica, MA), and then incubated with BPH-1 cells for 24 h. The viral-infected BPH-1 cells contain green fluorescence protein (GFP), so the BPH-AR or BPH-vector stable cells were obtained by the flow cytometry sorting.



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