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ascorbic acid  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ascorbic acid
    Ascorbic Acid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ascorbic+acid/L-(%2B)-Ascorbic+acid%2C+98%2B%25/pm42354430-215-44-46
    Average 94 stars, based on 1 article reviews
    ascorbic acid - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Modification:

    Article Title: Ice Templated PEG-Alginate Double-Network Cryogels with Tunable Mechanics and Degradation for Soft Tissue Engineering.
    Article Snippet: 8-arm polyethylene glycol acrylate (8-arm PEG acrylate, 10 kDa; Jenkem Technology, Plano, TX, USA), sodium alginate with an M/G ratio of 0.6 (Protanal® LF 10/60, IFF Pharma Solutions, Wilmington, DE, USA), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC; Thermo Fisher Scientific, Waltham, MA, USA), N-hydroxysuccinimide (NHS; Tokyo Chemical Industry, Tokyo, Japan), 2-(N-morpholino) ethanesulfonic acid buffer (MES, 1.0 M, pH 7.0; Thermo Fisher Scientific), and adipic acid dihydrazide (AAD; Tokyo Chemical Industry). .. Dithiothreitol (DTT) and ethylene glycol bis(mercaptoacetate) (EGBMA), Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12, 1:1), fetal bovine serum (FBS), penicillin–streptomycin (PS), L-glutamine, Dulbecco’s phosphate-buffered saline (DPBS), and 0.05% trypsin–EDTA, Rat tail collagen type I, The LIVE/DEAD Viability/Cytotoxicity Kit, 4′,6-diamidino-2-phenylindole (DAPI), and 4% paraformaldehyde (PFA), ascorbic acid (Thermo Fisher Scientific). .. Dexamethasone (Gibco, Grand Island, NY, USA), insulin–transferrin–selenium (ITS (1X), Sigma-Aldrich, Saint Louis, MO, USA), D1 mouse mesenchymal stem cells (ATCC, passage 11).

    Article Title: Isolation and Characterization of Marrow-Isolated Adult Multilineage Inducible (MIAMI) Cell-Derived Extracellular Vesicles Demonstrate Multifunctional Therapeutic Potential in Tissue Regeneration and Anti-Inflammatory Immunomodulation
    Article Snippet: .. Human bone marrow-derived MIAMI cells were cultured in DMEM (Dulbecco’s modified Eagle medium; Gibco, Waltham, MA, USA) supplemented with 1 g/L glucose, L-glutamine, 110 mg/L sodium pyruvate, 3% fetal bovine serum (FBS; Avantor), 1% penicillin–streptomycin (100 U/mL and 1000 U/mL, respectively; Gibco), 1% HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; Gibco), 50 μM ascorbic acid (Thermo Fisher Scientific, Waltham, MA, USA), and 0.05% of a mix of lipids solution. ..

    Saline:

    Article Title: Ice Templated PEG-Alginate Double-Network Cryogels with Tunable Mechanics and Degradation for Soft Tissue Engineering.
    Article Snippet: 8-arm polyethylene glycol acrylate (8-arm PEG acrylate, 10 kDa; Jenkem Technology, Plano, TX, USA), sodium alginate with an M/G ratio of 0.6 (Protanal® LF 10/60, IFF Pharma Solutions, Wilmington, DE, USA), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC; Thermo Fisher Scientific, Waltham, MA, USA), N-hydroxysuccinimide (NHS; Tokyo Chemical Industry, Tokyo, Japan), 2-(N-morpholino) ethanesulfonic acid buffer (MES, 1.0 M, pH 7.0; Thermo Fisher Scientific), and adipic acid dihydrazide (AAD; Tokyo Chemical Industry). .. Dithiothreitol (DTT) and ethylene glycol bis(mercaptoacetate) (EGBMA), Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12, 1:1), fetal bovine serum (FBS), penicillin–streptomycin (PS), L-glutamine, Dulbecco’s phosphate-buffered saline (DPBS), and 0.05% trypsin–EDTA, Rat tail collagen type I, The LIVE/DEAD Viability/Cytotoxicity Kit, 4′,6-diamidino-2-phenylindole (DAPI), and 4% paraformaldehyde (PFA), ascorbic acid (Thermo Fisher Scientific). .. Dexamethasone (Gibco, Grand Island, NY, USA), insulin–transferrin–selenium (ITS (1X), Sigma-Aldrich, Saint Louis, MO, USA), D1 mouse mesenchymal stem cells (ATCC, passage 11).

    Cell Culture:

    Article Title: Isolation and Characterization of Marrow-Isolated Adult Multilineage Inducible (MIAMI) Cell-Derived Extracellular Vesicles Demonstrate Multifunctional Therapeutic Potential in Tissue Regeneration and Anti-Inflammatory Immunomodulation
    Article Snippet: .. Human bone marrow-derived MIAMI cells were cultured in DMEM (Dulbecco’s modified Eagle medium; Gibco, Waltham, MA, USA) supplemented with 1 g/L glucose, L-glutamine, 110 mg/L sodium pyruvate, 3% fetal bovine serum (FBS; Avantor), 1% penicillin–streptomycin (100 U/mL and 1000 U/mL, respectively; Gibco), 1% HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; Gibco), 50 μM ascorbic acid (Thermo Fisher Scientific, Waltham, MA, USA), and 0.05% of a mix of lipids solution. ..

    Article Title: Functional maturation of enteric neurons derived from human induced pluripotent stem cells.
    Article Snippet: On D15 of differentiation, cells were dissociated with Accutase and plated on 6-well plastic plates, glass coverslip dishes (Ibidi) or 8-well chamber slides (ThermoFisher) coated with poly-L-ornithine (PO; 15 μg/mL; Sigma Aldrich), laminin (LN; 2 μg/mL; R&D Systems) and fibronectin (FN; 2 μg/mL; Corning) at a density of 100,000 cells per cm 2 . .. Cells were cultured in NM containing N2 supplement (10 μL/mL), B27 supplement minus Vitamin A (20 μL/mL), GlutaMax (10 μL/mL), MEM NEAA (10 μL/mL) and Ascorbic Acid (100 μM; ThermoFisher Scientific) and glial derived neurotrophic factor (GDNF; 10 ng/mL; Peprotech), with media changes every 2 days. ..

    other:

    Article Title: Modelling synaptic dysfunction in childhood dementia using human iPSC-derived cortical networks
    Article Snippet: NMM consisted of BrainPhys neuronal medium supplemented with N2 Supplement A (STEMCELL Technologies), SM1 without vitamin A (STEMCELL Technologies), 200 nM ascorbic acid, 1.2 nM laminin (1 μg/ml; Gibco), 20 ng of BDNF (STEMCELL Technologies), 20 ng of GDNF (STEMCELL Technologies), 10 ng/mL IGF (STEMCELL Technologies), and 0.5 mM dibutyryl cyclic adenosine 50-monophosphate.

    Article Title: Biosynthesis of 14-membered cyclopeptide alkaloids via non-heme-iron- and 2-oxoglutarate-dependent oxidative decarboxylation
    Article Snippet: The protein concentration was estimated by OD 280 using a Thermo Scientific NanoDrop One microvolume ultraviolet-visible light spectrophotometer based on the calculated ExPASy ProtParam extinction coefficient .

    Article Title: Photo-click Decellularized Matrix Hydrogels for Generating Pancreatic Ductal Organoids
    Article Snippet: Briefly, basal media BE3 containing MCDB131 (Thermo) with 2 mM L-Glutamine, 1.754 g/l Sodium bicarbonate, 0.44 g/l glucose (Sigma), 0.5% ITS-X (GIBCO), 44 mg/l L-Ascorbic acid and 2% fatty acid free BSA was dissolved and filtered through a 0.22 μm filter.

    Derivative Assay:

    Article Title: Functional maturation of enteric neurons derived from human induced pluripotent stem cells.
    Article Snippet: On D15 of differentiation, cells were dissociated with Accutase and plated on 6-well plastic plates, glass coverslip dishes (Ibidi) or 8-well chamber slides (ThermoFisher) coated with poly-L-ornithine (PO; 15 μg/mL; Sigma Aldrich), laminin (LN; 2 μg/mL; R&D Systems) and fibronectin (FN; 2 μg/mL; Corning) at a density of 100,000 cells per cm 2 . .. Cells were cultured in NM containing N2 supplement (10 μL/mL), B27 supplement minus Vitamin A (20 μL/mL), GlutaMax (10 μL/mL), MEM NEAA (10 μL/mL) and Ascorbic Acid (100 μM; ThermoFisher Scientific) and glial derived neurotrophic factor (GDNF; 10 ng/mL; Peprotech), with media changes every 2 days. ..



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