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Biochrom ascorbate-2-phosphate
Ascorbate 2 Phosphate, supplied by Biochrom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ascorbate2-phosphate/ascorbate+2+phosphate/pmc07408646-130-54-59
Average 90 stars, based on 1 article reviews
ascorbate-2-phosphate - by Bioz Stars, 2026-11
90/100 stars

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Article Title: Biocompatibility and intradiscal application of a thermoreversible celecoxib-loaded poly-N-isopropylacrylamide MgFe-layered double hydroxide hydrogel in a canine model
Article Snippet: The constructs were then cultured in 12-well plates in high-glucose Dulbecco’s modified Eagle’s medium (hgDMEM; Gibco, Life Technologies Europe, Bleiswijk, The Netherlands) with 20 % fetal bovine serum (Greiner Bio-One, Alphen aan Den Rijn, The Netherlands), 0.1 % amphotericin (Sigma-Aldrich Chemie B.V.), 1 % Pen-Strep (Biochrom GmbH, Berlin, Germany), 1 % nonessential amino acids (Lonza, Basel, Switzerland) 1 % essential amino acids (Lonza), and 50 mg/ml ascorbate 2-phosphate (Biochrom).

Article Title: Heparin Anticoagulant for Human Bone Marrow Does Not Influence In Vitro Performance of Human Mesenchymal Stromal Cells
Article Snippet: After another centrifugation step (5 min at 200× g ), the medium was discarded and the generated pellet was mixed with induction medium made of DMEM with high glucose content (Biochrom, FG0435) with 20 mM HEPES, 1% (100 U/mL / 100 μg/mL) penicillin/streptomycin (Biochrom), 100 nM dexamethasone, 1x ITS + (Sigma I3146), 170 μM ascorbate-2-phosphate, 1 mM sodium pyruvate (Biochrom), 350 μM proline (Carl Roth) and 10 ng/mL human recombinant TGF-β 3 ( Escherichia coli , PeproTech, Hamburg, Germany).

Article Title: Improved adipogenic in vitro differentiation: comparison of different adipogenic cell culture media on human fat and bone stroma cells for fat tissue engineering
Article Snippet: DMEM/Ham's F-12 with L-glutamine was the basis for the SAM supplemented with 10% fetal calf serum, 100 IU/ml penicillin, 100 μg/ml streptomycin, 2 mM L-glutamine, 1 mM ascorbate-2-phosphate (all obtained from Biochrom AG).

Article Title: Improved adipogenic in vitro differentiation: comparison of different adipogenic cell culture media on human fat and bone stroma cells for fat tissue engineering
Article Snippet: CM had been assembled of Dulbecco's modified Eagle's medium (DMEM) with low glucose (1,000 mg/l) supplemented with fetal calf serum 10%, 100 IU/ml penicillin, 100 μg/ml streptomycin, 25 μg/ml amphotericin, 2 mM L-glutamine and 1 mM ascorbate-2-phosphate (all obtained from Biochrom AG, Berlin, Germany) ( ).

Cell Culture:

Article Title: Local inhibition of angiogenesis results in an atrophic non-union in a rat osteotomy model.
Article Snippet: For cell isolation, bone was harvested during knee joint replacement and the trabecular bone was minced into little pieces followed by overnight digestion with collagenase Type II (Biochrom, Berlin, Germany) according to established protocols (Robey and Termine, 1985). .. The cells were cultured at 37 °C, 5 % CO2, and 95 % humidity in MEME/F12 1:1 + 10 % foetal calf serum (FCS) + 1 % penicillin/streptomycin (PS), + 0.05 mM L-ascorbate-2-Phosphate, + 0.05 mM ß-glycerolPhosphate (Biochrom) (POB), or MCDB 131 media (Invitrogen, Darmstadt, Germany) + 2 mM L-Glutamine, 5 % FCS, 1 % PS (Biochrom) + freshly supplemented 1 μg/mL hydrocortisone (Sigma-Aldrich) (HMEC-1), respectively. ..



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