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arrayworx biochip reader  (Applied Precision Inc)

 
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    Structured Review

    Applied Precision Inc arrayworx biochip reader
    Arrayworx Biochip Reader, supplied by Applied Precision Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/arrayworx+e+biochip+reader/arrayworx+biochip+reader/pm17509075-168-6-9
    Average 86 stars, based on 1 article reviews
    arrayworx biochip reader - by Bioz Stars, 2026-10
    86/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Creation of Stepwise Concentration Gradient in Picoliter Droplets for Parallel Reactions of Matrix Metalloproteinase II and IX
    Article Snippet: .. Image acquisition and processing A series of fluorescence images of the enzyme reactions were acquired with a modified biochip reader (arrayWoRx ® , Applied Precision, WA). .. All fluorescence images shown in were digitized and analyzed by using the time series analyzer of ImageJ software (NIH, USA).

    Article Title: Methods and compositions for phototransfer
    Article Snippet: .. Microarray slides were dried by centrifugation in a padded tube and, prior to MALDI-TOF, the slide was imaged for fluorescence in the ArrayWoRxe BioChip reader (Applied Precision, LLC, Issaquah, Wash.). ..

    Article Title: Creation of Stepwise Concentration Gradient in Picoliter Droplets for Parallel Reactions of Matrix Metalloproteinase II and IX
    Article Snippet: .. A series of fluorescence images of the enzyme reactions were acquired with a modified biochip reader (arrayWoRx ® , Applied Precision, WA). ..

    Modification:

    Article Title: Creation of Stepwise Concentration Gradient in Picoliter Droplets for Parallel Reactions of Matrix Metalloproteinase II and IX
    Article Snippet: .. Image acquisition and processing A series of fluorescence images of the enzyme reactions were acquired with a modified biochip reader (arrayWoRx ® , Applied Precision, WA). .. All fluorescence images shown in were digitized and analyzed by using the time series analyzer of ImageJ software (NIH, USA).

    Article Title: Creation of Stepwise Concentration Gradient in Picoliter Droplets for Parallel Reactions of Matrix Metalloproteinase II and IX
    Article Snippet: .. A series of fluorescence images of the enzyme reactions were acquired with a modified biochip reader (arrayWoRx ® , Applied Precision, WA). ..

    other:

    Article Title: Screening of Pre-miRNA-155 Binding Peptides for Apoptosis Inducing Activity Using Peptide Microarrays.
    Article Snippet: MicroRNA-155, one of the most potent miRNAs that suppress apoptosis in human cancer, is overexpressed in numerous cancers, and it displays oncogenic activity.. Peptide microarrays, constructed by immobilizing 185 peptides containing the C-terminal hydrazide onto epoxide-derivatized glass slides, were employed to evaluate peptide binding properties of premiRNA-155 and to identify its binding peptides.. Two peptides, which were identified based on the results of peptide microarray and in vitro Dicer inhibition studies, were found to inhibit generation of mature miRNA-155 catalyzed by Dicer and to enhance expression of miRNA-155 target genes in cells.

    Microarray:

    Article Title: Methods and compositions for phototransfer
    Article Snippet: .. Microarray slides were dried by centrifugation in a padded tube and, prior to MALDI-TOF, the slide was imaged for fluorescence in the ArrayWoRxe BioChip reader (Applied Precision, LLC, Issaquah, Wash.). ..

    Centrifugation:

    Article Title: Methods and compositions for phototransfer
    Article Snippet: .. Microarray slides were dried by centrifugation in a padded tube and, prior to MALDI-TOF, the slide was imaged for fluorescence in the ArrayWoRxe BioChip reader (Applied Precision, LLC, Issaquah, Wash.). ..

    Software:

    Article Title: Methods and compositions for phototransfer
    Article Snippet: .. Photo-transferred spot diameters were measured using the software supplied by Applied Precision, LLC (Issaquah, Wash.) with their ArrayWoRxe BioChip reader. .. Spots in the entire 7 mm diameter printed area were enumerated using simple 2-D electrophoresis spot detection software (ImageQuant; Molecular Dynamics; Amersham Biosciences Corp., Piscataway, N.J.).



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    Image Search Results


    a) microfluidic design: the device consists of two PDMS layers: flow (blue) and control (red). The chip is an array of eight rows by 48 columns for 384 unit cells. Each unit cell is composed of: two antibody chambers divided by a reaction chamber (1–2), 4 MITOMI buttons (3), a valve that segregates the unit cells (4), a valve that separates antibody and reaction chambers (5) and a valve for releasing pressure in the antibody chambers (6). b) The PDMS chip is aligned to an epoxy-functionalized slide onto which primary and secondary antibodies were spotted. c) Assay details: schematic of the unit cell and cross section of a button region: i) functionalization of the surface: BSA-biotin is flowed though the chip followed by neutravidin. Next, the buttons are closed and BSA-biotin flowed again to passivate all neutravidin molecules except for those located underneath the MITOMI buttons, ii) the biotinylated primary antibody is allowed to diffuse into the MITOMI detection chamber and is bound by neutravidin immobilizing it in the MITOMI detection regions, iii) the sample is flown through the device and antigens are captured by the surface immobilized antibodies, iv) finally, the fluorescently labeled secondary antibody is allowed to diffuse into the MITOMI area, binds to the antigen if present, and is trapped by MITOMI. The entire device is then quantitated using a DNA microarray scanner.

    Journal: PLoS ONE

    Article Title: A Microfluidic Platform for High-Throughput Multiplexed Protein Quantitation

    doi: 10.1371/journal.pone.0117744

    Figure Lengend Snippet: a) microfluidic design: the device consists of two PDMS layers: flow (blue) and control (red). The chip is an array of eight rows by 48 columns for 384 unit cells. Each unit cell is composed of: two antibody chambers divided by a reaction chamber (1–2), 4 MITOMI buttons (3), a valve that segregates the unit cells (4), a valve that separates antibody and reaction chambers (5) and a valve for releasing pressure in the antibody chambers (6). b) The PDMS chip is aligned to an epoxy-functionalized slide onto which primary and secondary antibodies were spotted. c) Assay details: schematic of the unit cell and cross section of a button region: i) functionalization of the surface: BSA-biotin is flowed though the chip followed by neutravidin. Next, the buttons are closed and BSA-biotin flowed again to passivate all neutravidin molecules except for those located underneath the MITOMI buttons, ii) the biotinylated primary antibody is allowed to diffuse into the MITOMI detection chamber and is bound by neutravidin immobilizing it in the MITOMI detection regions, iii) the sample is flown through the device and antigens are captured by the surface immobilized antibodies, iv) finally, the fluorescently labeled secondary antibody is allowed to diffuse into the MITOMI area, binds to the antigen if present, and is trapped by MITOMI. The entire device is then quantitated using a DNA microarray scanner.

    Article Snippet: The microfluidic device was scanned using a fluorescent microarray scanner (ArrayWorx e-Biochip Reader, Applied Precision, USA).

    Techniques: Control, Labeling, Microarray