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Aushon Biosystems 2470 arrayer
2470 Arrayer, supplied by Aushon Biosystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/arrayer+2470/2470+arrayer/pm39695112-110-12-15
Average 90 stars, based on 1 article reviews
2470 arrayer - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

other:

Article Title: Mapping three-dimensional intratumor proteomic heterogeneity in uterine serous carcinoma by multiregion microsampling.
Article Snippet: Reverse phase protein microarray ET, ES, and BT lysates in the RPPA extraction/lysis buffer were boiled and used for microarray printing onto nitrocellulose-coated glass slides (Grace Bio-Labs) in technical triplicates using a 2470 Aushon Arrayer (Aushon BioSystems, Inc.), as previously described [17, 18].

Protein Concentration:

Article Title: A protein expression atlas on tissue samples and cell lines from cancer patients provides insights into tumor heterogeneity and dependencies
Article Snippet: The frozen tumors or cell pellets were lysed, and proteins were extracted using RPPA lysis buffer (containing 1% Triton X‐100, 50mM HEPES at pH 7.4, 150mM NaCl, 1.5mM MgCl 2 , 1mM EGTA, 100mM NaF, 10mM Na pyrophosphate, 1mM Na 3 VO 4 , 10% glycerol, and freshly incorporated protease and phosphatase inhibitors from Roche Applied Science (Cat. # 05056489001 and 04906837001, respectively) 61 . .. These lysates were adjusted for protein concentration and then serially diluted into five twofold dilutions using the same lysis buffer, followed by printing on nitrocellulose-coated slides using an Aushon Biosystems 2470 arrayer. .. The slides were then probed with ~500 pre-validated primary antibodies and detected with suitable biotinylated secondary antibodies: Goat anti-Rabbit IgG (Invitrogen, Cat#: 31822, Lot#: XC3537505, dilution: 1:1000), Goat anti-Mouse IgG (Vector Laboratories, Cat#: BA-9200, Lot#: ZD0801, dilution: 1:10000), or Rabbit anti-Goat IgG (Fisher Scientific, Cat#: NC9376096, Lot#: ZB0923, dilution: 1:10000).

Lysis:

Article Title: A protein expression atlas on tissue samples and cell lines from cancer patients provides insights into tumor heterogeneity and dependencies
Article Snippet: The frozen tumors or cell pellets were lysed, and proteins were extracted using RPPA lysis buffer (containing 1% Triton X‐100, 50mM HEPES at pH 7.4, 150mM NaCl, 1.5mM MgCl 2 , 1mM EGTA, 100mM NaF, 10mM Na pyrophosphate, 1mM Na 3 VO 4 , 10% glycerol, and freshly incorporated protease and phosphatase inhibitors from Roche Applied Science (Cat. # 05056489001 and 04906837001, respectively) 61 . .. These lysates were adjusted for protein concentration and then serially diluted into five twofold dilutions using the same lysis buffer, followed by printing on nitrocellulose-coated slides using an Aushon Biosystems 2470 arrayer. .. The slides were then probed with ~500 pre-validated primary antibodies and detected with suitable biotinylated secondary antibodies: Goat anti-Rabbit IgG (Invitrogen, Cat#: 31822, Lot#: XC3537505, dilution: 1:1000), Goat anti-Mouse IgG (Vector Laboratories, Cat#: BA-9200, Lot#: ZD0801, dilution: 1:10000), or Rabbit anti-Goat IgG (Fisher Scientific, Cat#: NC9376096, Lot#: ZB0923, dilution: 1:10000).



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