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humanmethylation27 bead array  (INFINIUM Inc)

 
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    INFINIUM Inc humanmethylation27 bead array
    DNA-methylation profiles were analyzed with the <t>HumanMethylation27</t> BeadChip microarray which represents 27,578 unique CpG sites. MSC derived from adipose tissue (MSC-AT) were compared with those derived from bone marrow, which was either aspirated from the iliac crest (MSC-BM) or taken from the caput femuris upon hip replacement (MSC-Hip). Unsupervised principal component analysis (PCA) clearly separated DNA-methylation profiles according to the tissue of origin in the first dimension (PC1), whereas the forth component (PC4) discerned early and late passage ( A ). Scatterplot comparison of passage 5 and passage 10 in MSC-AT revealed that 233 CpG sites are more than 15% hyper-methylated whereas 186 CpG sites are more than 15% hypo-methylated at passage 10 ( B ). Significance Analysis of Microarray (SAM) was used to select 517 senescence-associated CpG sites (FDR = 4.8%) and these are presented as a heatmap ( C ; data were divided by the mean of each row for graphical presentation).
    Humanmethylation27 Bead Array, supplied by INFINIUM Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/array-based+analysis+of+dna+methylation+patterns/infinium+humanmethylation450+beadchip/pmc03227452-14-9-8
    Average 90 stars, based on 1 article reviews
    humanmethylation27 bead array - by Bioz Stars, 2026-10
    90/100 stars

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    1) Product Images from "Replicative senescence of mesenchymal stem cells causes DNA-methylation changes which correlate with repressive histone marks"

    Article Title: Replicative senescence of mesenchymal stem cells causes DNA-methylation changes which correlate with repressive histone marks

    Journal: Aging (Albany NY)

    doi:

    DNA-methylation profiles were analyzed with the HumanMethylation27 BeadChip microarray which represents 27,578 unique CpG sites. MSC derived from adipose tissue (MSC-AT) were compared with those derived from bone marrow, which was either aspirated from the iliac crest (MSC-BM) or taken from the caput femuris upon hip replacement (MSC-Hip). Unsupervised principal component analysis (PCA) clearly separated DNA-methylation profiles according to the tissue of origin in the first dimension (PC1), whereas the forth component (PC4) discerned early and late passage ( A ). Scatterplot comparison of passage 5 and passage 10 in MSC-AT revealed that 233 CpG sites are more than 15% hyper-methylated whereas 186 CpG sites are more than 15% hypo-methylated at passage 10 ( B ). Significance Analysis of Microarray (SAM) was used to select 517 senescence-associated CpG sites (FDR = 4.8%) and these are presented as a heatmap ( C ; data were divided by the mean of each row for graphical presentation).
    Figure Legend Snippet: DNA-methylation profiles were analyzed with the HumanMethylation27 BeadChip microarray which represents 27,578 unique CpG sites. MSC derived from adipose tissue (MSC-AT) were compared with those derived from bone marrow, which was either aspirated from the iliac crest (MSC-BM) or taken from the caput femuris upon hip replacement (MSC-Hip). Unsupervised principal component analysis (PCA) clearly separated DNA-methylation profiles according to the tissue of origin in the first dimension (PC1), whereas the forth component (PC4) discerned early and late passage ( A ). Scatterplot comparison of passage 5 and passage 10 in MSC-AT revealed that 233 CpG sites are more than 15% hyper-methylated whereas 186 CpG sites are more than 15% hypo-methylated at passage 10 ( B ). Significance Analysis of Microarray (SAM) was used to select 517 senescence-associated CpG sites (FDR = 4.8%) and these are presented as a heatmap ( C ; data were divided by the mean of each row for graphical presentation).

    Techniques Used: DNA Methylation Assay, Microarray, Derivative Assay, Methylation

    Related Articles

    DNA Methylation Assay:

    Article Title: Sex differences in resting skeletal muscle and the acute and long-term response to endurance exercise in individuals with overweight and obesity
    Article Snippet: The bisulfite treatment and hybridization of the DNA samples were carried out by Life & Brain (Bonn, Germany). .. DNA methylation levels were determined by the Infinium® MethylationEPIC BeadChip version 1. ..

    Article Title: Convergent DNA Methylation Abnormalities at Bivalent Chromatin in Human Growth Disorders
    Article Snippet: .. DNA methylation data from blood of individuals with DNMT3A- or NSD1-associated growth syndromes, generated using the Infinium HumanMethylation450K or EPIC arrays, were obtained from published studies [ ], [ ], [ ] ( Supplemental Table 1). ..

    Article Title: Colon cancer methylation markers and uses thereof
    Article Snippet: .. DNA methylation data were obtained from the TCGA analysis of about 450,000 sites generated using the Infinium 450K Methylation Array. ..

    Article Title: Supplementary Materials Association between genome-wide epigenetic and genetic alterations in breast cancer tissue and response to HER2-targeted therapies in HER2-positive breast cancer patients: new findings and a systematic review
    Article Snippet: .. Summary characteristics of one study of genome-wide DNA methylation measured in breast cancer tissue and risk of recurrence among HER2-positive, trastuzumab- treated breast cancer patients (n = 1) Study design Retrospective study: Furrer [2022] Breast cancer patients Number of patients: 12 Mean age: 51.3 y Postmenopausal patients: 67% ER-positive breast cancer: 67% DNA methylation Measurement method: Infinium HumanMethylation450 BeadChip array Tissue used: FFPE Tumor cell fraction: ≥ 70% Outcome DFS DFS: Disease free survival; ER: estrogen receptor; FFPE: formalin-fixed, paraffin-embedded; n: number of studies; y: years. ..

    Article Title: Liver cancer methylation markers and uses thereof
    Article Snippet: .. DNA methylation data were obtained from the TCGA analysis of about 485,000 sites generated using the Infinium 450K Methylation Array. ..

    Article Title: Colon cancer methylation markers and uses thereof
    Article Snippet: .. DNA methylation data were obtained from the TCGA analysis of about 27,000 sites generated using the Infinium 450K Methylation Array. ..

    Generated:

    Article Title: Convergent DNA Methylation Abnormalities at Bivalent Chromatin in Human Growth Disorders
    Article Snippet: .. DNA methylation data from blood of individuals with DNMT3A- or NSD1-associated growth syndromes, generated using the Infinium HumanMethylation450K or EPIC arrays, were obtained from published studies [ ], [ ], [ ] ( Supplemental Table 1). ..

    Article Title: Colon cancer methylation markers and uses thereof
    Article Snippet: .. DNA methylation data were obtained from the TCGA analysis of about 450,000 sites generated using the Infinium 450K Methylation Array. ..

    Article Title: Liver cancer methylation markers and uses thereof
    Article Snippet: .. DNA methylation data were obtained from the TCGA analysis of about 485,000 sites generated using the Infinium 450K Methylation Array. ..

    Article Title: Colon cancer methylation markers and uses thereof
    Article Snippet: .. DNA methylation data were obtained from the TCGA analysis of about 27,000 sites generated using the Infinium 450K Methylation Array. ..

    Methylation:

    Article Title: Colon cancer methylation markers and uses thereof
    Article Snippet: .. DNA methylation data were obtained from the TCGA analysis of about 450,000 sites generated using the Infinium 450K Methylation Array. ..

    Article Title: Liver cancer methylation markers and uses thereof
    Article Snippet: .. DNA methylation data were obtained from the TCGA analysis of about 485,000 sites generated using the Infinium 450K Methylation Array. ..

    Article Title: Colon cancer methylation markers and uses thereof
    Article Snippet: .. DNA methylation data were obtained from the TCGA analysis of about 27,000 sites generated using the Infinium 450K Methylation Array. ..

    Genome Wide:

    Article Title: Supplementary Materials Association between genome-wide epigenetic and genetic alterations in breast cancer tissue and response to HER2-targeted therapies in HER2-positive breast cancer patients: new findings and a systematic review
    Article Snippet: .. Summary characteristics of one study of genome-wide DNA methylation measured in breast cancer tissue and risk of recurrence among HER2-positive, trastuzumab- treated breast cancer patients (n = 1) Study design Retrospective study: Furrer [2022] Breast cancer patients Number of patients: 12 Mean age: 51.3 y Postmenopausal patients: 67% ER-positive breast cancer: 67% DNA methylation Measurement method: Infinium HumanMethylation450 BeadChip array Tissue used: FFPE Tumor cell fraction: ≥ 70% Outcome DFS DFS: Disease free survival; ER: estrogen receptor; FFPE: formalin-fixed, paraffin-embedded; n: number of studies; y: years. ..

    Formalin-fixed Paraffin-Embedded:

    Article Title: Supplementary Materials Association between genome-wide epigenetic and genetic alterations in breast cancer tissue and response to HER2-targeted therapies in HER2-positive breast cancer patients: new findings and a systematic review
    Article Snippet: .. Summary characteristics of one study of genome-wide DNA methylation measured in breast cancer tissue and risk of recurrence among HER2-positive, trastuzumab- treated breast cancer patients (n = 1) Study design Retrospective study: Furrer [2022] Breast cancer patients Number of patients: 12 Mean age: 51.3 y Postmenopausal patients: 67% ER-positive breast cancer: 67% DNA methylation Measurement method: Infinium HumanMethylation450 BeadChip array Tissue used: FFPE Tumor cell fraction: ≥ 70% Outcome DFS DFS: Disease free survival; ER: estrogen receptor; FFPE: formalin-fixed, paraffin-embedded; n: number of studies; y: years. ..



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