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deparaffinized tissue array slide  (SuperBioChips)

 
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    SuperBioChips deparaffinized tissue array slide
    Deparaffinized Tissue Array Slide, supplied by SuperBioChips, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/array+slides/deparaffinized+tissue+array+slide/pmc04724813-16-2-11
    Average 90 stars, based on 1 article reviews
    deparaffinized tissue array slide - by Bioz Stars, 2026-10
    90/100 stars

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    Incubation:

    Article Title: Bauhinia purprea agglutinin ‐modified liposomes for human prostate cancer treatment
    Article Snippet: Human prostate cancer cells lines DU145 and LNCaP were cultured in RPMI‐1640 medium (Wako Pure Chemical Industries, Osaka, Japan) supplemented with 10% heat‐inactivated FBS (AusGeneX Pty Ltd, Brisbane, Australia), 100 μg/mL streptomycin (MP Biomedicals, Irvine, CA, USA) and 100 units/mL penicillin (MP Biomedicals) at 37°C in a humidified atmosphere with 5% CO 2 . .. A deparaffinized tissue array slide (human normal prostate and cancer tissue; SuperBioChips Laboratories, Seoul, Korea) was blocked with 1% BSA‐PBS for 30 min. Then, biotin‐conjugated BPA (50 μg/mL) was added to the array slide; and incubation was carried out for 1 h at room temperature. .. After a wash, the slide was next incubated with streptavidin‐conjugated HRP (5 μg/mL; Vector Laboratories, Burlingame, CA, USA) for 30 min. To visualize the bound BPA molecules, we incubated the array slide for approximately 5 min in the solution provided in a DAB liquid substrate system (Sigma‐Aldrich Co. LLC, St. Louis, MO, USA).



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    Image Search Results


    Characterization of the autologous hair follicle secretome (aHFS). ( A ) Semi-quantitative analysis of selected protein analytes in aHFS using a Quantibody ® glass slide-based array (RayBiotech). Data represent arbitrary intensity values normalized to background ( n = 3). ( B ) Concentrations of selected growth factors and cytokines in aHFS ( n = 6). Nanoparticle tracking analysis (NTA) showing particle concentration ( C ) and modal size distribution ( D ) in aHFS samples ( n = 146). ( E ) ONI super-resolution image of exosomes present in aHFS lyophilized secretome. Exosomes were triple-labeled for tetraspanin markers CD81 (purple), CD63 (yellow), and CD9 (teal). ( F ) Representative brightfield images from a scratch wound assay using primary keratinocytes treated with commercial media or 25% aHFS, imaged at 0 and 24 h. ( G ) Quantification of wound closure over time. Data are shown as mean ± SEM. ** p < 0.01 vs. control.

    Journal: International Journal of Molecular Sciences

    Article Title: The Autologous Hair Follicle and Its Secretome: A Multipotent Source for Cell-Based and Cell-Free Regenerative Therapies

    doi: 10.3390/ijms27104183

    Figure Lengend Snippet: Characterization of the autologous hair follicle secretome (aHFS). ( A ) Semi-quantitative analysis of selected protein analytes in aHFS using a Quantibody ® glass slide-based array (RayBiotech). Data represent arbitrary intensity values normalized to background ( n = 3). ( B ) Concentrations of selected growth factors and cytokines in aHFS ( n = 6). Nanoparticle tracking analysis (NTA) showing particle concentration ( C ) and modal size distribution ( D ) in aHFS samples ( n = 146). ( E ) ONI super-resolution image of exosomes present in aHFS lyophilized secretome. Exosomes were triple-labeled for tetraspanin markers CD81 (purple), CD63 (yellow), and CD9 (teal). ( F ) Representative brightfield images from a scratch wound assay using primary keratinocytes treated with commercial media or 25% aHFS, imaged at 0 and 24 h. ( G ) Quantification of wound closure over time. Data are shown as mean ± SEM. ** p < 0.01 vs. control.

    Article Snippet: A semi-quantitative Quantibody ® glass slide-based cytokine array (RayBiotech, Peachtree Corners, GA, USA; Cat. #QAM-CAA-4000) was utilized to profile growth factors and cytokines.

    Techniques: Concentration Assay, Labeling, Scratch Wound Assay Assay, Control