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array membranes  (Bio-Rad)


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    Bio-Rad array membranes
    Array Membranes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 30458 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/array+membranes/Nitrocellulose+Membranes/pmc12531157-83-0-28
    Average 99 stars, based on 30458 article reviews
    array membranes - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: An edge β‐strand mutation turns ubiquitin into a pore‐forming amyloid
    Article Snippet: .. Aliquots (2 μL) of 10 μM protein solutions were spotted onto 0.45 μm nitrocellulose membranes (Bio‐Rad) and air‐dried for 15 min. Membranes were blocked with 10% non‐fat dry milk in TBS‐T (20 mM Tris, 0.8% NaCl, 0.01% Tween‐20, pH 7.6) for 1 h and incubated overnight at 4°C with A11 anti‐oligomer primary antibody (Invitrogen, 1:2000 in 5% milk/TBS‐T) (Kayed et al., ). .. After three 10‐min washes, membranes were incubated with goat anti‐rabbit IgG‐HRP conjugate (Santa Cruz Biotechnology, 1:5000 in 5% milk/TBS‐T) for 1 h, washed, and developed with ECL reagents (Thermo Scientific).

    SDS Page:

    Article Title: Glycosylated Chitosan Inhibits Pancreatic Cancer Metastasis by Blocking the Caveolin Signaling Pathway.
    Article Snippet: The following primary mAbs were used: α-actinin rabbit mAb, vinculin rabbit mAb, caveolin-1 rabbit mAb, β-actin mouse mAb (Cell Signaling, Beverly, MA, USA), paxillin rabbit mAb, FAK rabbit mAb, and FAK (phospho Y397) rabbit mAb (Abcam, Cambridge, MA, USA). .. Protein samples were subjected to SDS-PAGE and transferred to nitrocellulose membranes using standard methods and commercial reagents (Bio-Rad, Hercules, CA, USA). ..

    Article Title: Novel ADPRS Missense Variant (p.Leu162Pro) Causes Stress-Induced Childhood-Onset Neurodegeneration With Ataxia and Seizures
    Article Snippet: .. For each sample, 15–40 μg of protein were loaded into SDS-PAGE gels, then transferred to nitrocellulose membranes (Bio-Rad, CA). .. Membranes were stained with Ponceau S (Sigma-Aldrich, MO) for visualization and loading control.

    Staining:

    Article Title: NAD + supply and redox state limit developmental speed in the Drosophila eye.
    Article Snippet: Inhibitors of PARG (1 μM; PDD00017273, Sigma), and PARP activities (1 μM; Olaparib, LKT LABS) were added in the lysis buffer. .. Following denaturation (100 °C for 10 min), proteins were separated on Mini-PROTEAN TGX Stain-Free Gels, 8-16% (Bio-Rad) and transferred to nitrocellulose membranes (Bio-Rad) overnight at 4 °C. ..

    Protein Concentration:

    Article Title: Identification of PLXNC1 as a novel biomarker for consensus molecular subtype 4 in colorectal cancer
    Article Snippet: Total protein from CRC cells treated with siRNA-NC or siRNA-PLXNC1 for 48 h was collected using lysis buffer (Cwbio, Beijing, China) supplemented with protease inhibitor cocktail (Roche, Basel, Switzerland). .. The protein concentration was quantified through a bicinchoninic acid protein assay (Pierce Biotechnology, Rockford, Illinois, USA), after which the protein was separated by 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis and transferred onto nitrocellulose membranes (Bio-Rad, Hercules, California, USA). .. The membranes were incubated with specific primary antibodies against SNAIL (13099-1-AP, Proteintech, 1:500), vimentin (10366-1-AP, Proteintech, 1:10000), E-cadherin (20874-1-AP, Proteintech, 1:10000), and GAPDH (#5174, Cell Signaling, 1:1000) at 4 °C overnight, followed by incubation with the appropriate secondary antibodies (S8002, Sudgen, 1:10000).

    Bicinchoninic Acid Protein Assay:

    Article Title: Identification of PLXNC1 as a novel biomarker for consensus molecular subtype 4 in colorectal cancer
    Article Snippet: Total protein from CRC cells treated with siRNA-NC or siRNA-PLXNC1 for 48 h was collected using lysis buffer (Cwbio, Beijing, China) supplemented with protease inhibitor cocktail (Roche, Basel, Switzerland). .. The protein concentration was quantified through a bicinchoninic acid protein assay (Pierce Biotechnology, Rockford, Illinois, USA), after which the protein was separated by 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis and transferred onto nitrocellulose membranes (Bio-Rad, Hercules, California, USA). .. The membranes were incubated with specific primary antibodies against SNAIL (13099-1-AP, Proteintech, 1:500), vimentin (10366-1-AP, Proteintech, 1:10000), E-cadherin (20874-1-AP, Proteintech, 1:10000), and GAPDH (#5174, Cell Signaling, 1:1000) at 4 °C overnight, followed by incubation with the appropriate secondary antibodies (S8002, Sudgen, 1:10000).

    Nucleic Acid Electrophoresis:

    Article Title: Identification of PLXNC1 as a novel biomarker for consensus molecular subtype 4 in colorectal cancer
    Article Snippet: Total protein from CRC cells treated with siRNA-NC or siRNA-PLXNC1 for 48 h was collected using lysis buffer (Cwbio, Beijing, China) supplemented with protease inhibitor cocktail (Roche, Basel, Switzerland). .. The protein concentration was quantified through a bicinchoninic acid protein assay (Pierce Biotechnology, Rockford, Illinois, USA), after which the protein was separated by 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis and transferred onto nitrocellulose membranes (Bio-Rad, Hercules, California, USA). .. The membranes were incubated with specific primary antibodies against SNAIL (13099-1-AP, Proteintech, 1:500), vimentin (10366-1-AP, Proteintech, 1:10000), E-cadherin (20874-1-AP, Proteintech, 1:10000), and GAPDH (#5174, Cell Signaling, 1:1000) at 4 °C overnight, followed by incubation with the appropriate secondary antibodies (S8002, Sudgen, 1:10000).



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    Benzo-17R-RvD2 mimetic in peritonitis limits PMN tissue infiltration and regulates cytokines Mice were administered with vehicle or benzo-17R-RvD2 (1 ng/mouse) together with zymosan A (1 mg/mouse, i.p.) and exudates collected at 4 h. Total leukocytes were enumerated and composition determined by flow cytometry (see B for gating strategy). (A) Exudate PMN numbers; mean ± SEM, n = 4 mice each group, ∗∗ p < 0.01. (B) Proteome analysis was carried out with inflammatory exudates from four individual mice in each group using Proteome Profiler cytokine arrays, which contains 40 cytokines, chemokines, and acute phase proteins. Orthogonal Partial Least Squares Discriminant Analysis (PLS-DA): the score plot (each dot represents the cytokine profile from each mouse) shows clustering among groups (i.e., peritonitis vs. peritonitis plus benzo-17R-RvD2). The loading plot demonstrates correlations in which the measured cytokines contribute to the cluster separation in the score plot. (C) Relative intensities of cytokines, chemokines, and acute phase proteins. Proteome arrays consist of 40 proteins. Among them 18 were present in the peritoneal exudates. Results are mean ± SEM; relative abundance from n = 4 individual mouse exudates, ∗ p < 0.05, ∗∗ p < 0.01; peritonitis plus benzo-17R-RvD2 versus peritonitis alone; Student’s t test.

    Journal: iScience

    Article Title: Nouveau benzo-mimetics of 17R-Resolvin D2 are potent resolution agonists for inflammation

    doi: 10.1016/j.isci.2025.114201

    Figure Lengend Snippet: Benzo-17R-RvD2 mimetic in peritonitis limits PMN tissue infiltration and regulates cytokines Mice were administered with vehicle or benzo-17R-RvD2 (1 ng/mouse) together with zymosan A (1 mg/mouse, i.p.) and exudates collected at 4 h. Total leukocytes were enumerated and composition determined by flow cytometry (see B for gating strategy). (A) Exudate PMN numbers; mean ± SEM, n = 4 mice each group, ∗∗ p < 0.01. (B) Proteome analysis was carried out with inflammatory exudates from four individual mice in each group using Proteome Profiler cytokine arrays, which contains 40 cytokines, chemokines, and acute phase proteins. Orthogonal Partial Least Squares Discriminant Analysis (PLS-DA): the score plot (each dot represents the cytokine profile from each mouse) shows clustering among groups (i.e., peritonitis vs. peritonitis plus benzo-17R-RvD2). The loading plot demonstrates correlations in which the measured cytokines contribute to the cluster separation in the score plot. (C) Relative intensities of cytokines, chemokines, and acute phase proteins. Proteome arrays consist of 40 proteins. Among them 18 were present in the peritoneal exudates. Results are mean ± SEM; relative abundance from n = 4 individual mouse exudates, ∗ p < 0.05, ∗∗ p < 0.01; peritonitis plus benzo-17R-RvD2 versus peritonitis alone; Student’s t test.

    Article Snippet: The supernatant (750 μl) was incubated with the pre-coated Proteome Profiler array membranes (ARY006, R&D Systems) and processed according to the manufacturer’s instructions.

    Techniques: Flow Cytometry