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lipid arrays  (Echelon Biosciences)


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    Structured Review

    Echelon Biosciences lipid arrays
    Lipid Arrays, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 96/100, based on 446 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/array+membranes/Membrane+Lipid+Strips/pm40983659-660-12-14
    Average 96 stars, based on 446 article reviews
    lipid arrays - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Membrane:

    Article Title: Two Pleckstrin homology domains scaffold SNAP-47-containing SNARE complexes
    Article Snippet: .. Echelon Biosciences Membrane Lipid Strips were blocked for 1 h with TBST containing 3% fatty-acid-poor BSA before being incubated with purified PH1 or PH2 (5 μg/mL) for an additional 1 h. Following incubation, strips were washed with TBST and incubated with 6×-His Tag Monoclonal Antibody, HRP (1:900) for 1 h. Strips were then washed with TBST and developed using SuperSignal West Pico PLUS Chemiluminescent Substrate before imaging. ..

    Article Title: Interaction of lifeguard (LFG/FAIM2) with phosphoinositides is associated with altered intracellular calcium homeostasis in breast cancer cells.
    Article Snippet: .. The Interaction of LFG with cellular membranes, specifically lipids, was assessed with the use of Membrane Lipid Strips (Product P-6002) purchased from Echelon Biosciences Inc (Salt Lake City, UT, USA). ..

    Article Title: Multifaceted antimicrobial mechanisms of NCR147-derived peptides from Medicago truncatula
    Article Snippet: .. The membrane lipid strips (catalog No. P-6002) purchased from Echelon Bioscience (Salt Lake City, UT, United States) were used according to the recommended protocol. .. Briefly, the membranes were incubated with NCR147 25-36 -StrepII or NCR147 25-36 W 33,35 W F -StrepII at 10 μg/mL for 1 h and after washing the strips, the binding of Strep II tagged peptides to lipids was detected with StrepTactin-horseradish peroxidase (HRP) conjugate (BioRad Cat. No. 1610380) using chemiluminescence of HRP substrate with ECL Prime Western Blotting Detection Reagent (Cytiva Cat. No. GERPN2236).

    Article Title: Localized phosphoinositide metabolism regulates STIM1/ORAI1 fast inactivation
    Article Snippet: .. 624 Lipid-protein interaction assay 625 Recombinant GST-tagged MTMR7 was incubated with Membrane Lipid Strips (P-6002, Echelon 626 Biosciences), which contain pre-dotted spots of 15 distinct membrane lipids. ..

    Article Title: Localized phosphoinositide metabolism regulates STIM1/ORAI1 fast inactivation
    Article Snippet: .. Recombinant GST-tagged MTMR7 was incubated with Membrane Lipid Strips (P-6002, Echelon Biosciences), which contain pre-dotted spots of 15 distinct membrane lipids. ..

    Article Title: FtsK with a unique N-terminal extension is involved in coordinating the final steps of chromosome segregation with asymmetrical division in mycobacterial cells
    Article Snippet: .. Lipid-protein interaction assays were performed using membrane lipid strips (Echelon Biosciences, P-6002) according to the manufacturer’s instructions. .. Briefly, 2 μl of a goat anti-mouse IgG secondary antibody conjugated to HRP (Invitrogen) and 2 μl of the His-nFtsK-FLAG protein were spotted onto the membrane as positive controls.

    Incubation:

    Article Title: Two Pleckstrin homology domains scaffold SNAP-47-containing SNARE complexes
    Article Snippet: .. Echelon Biosciences Membrane Lipid Strips were blocked for 1 h with TBST containing 3% fatty-acid-poor BSA before being incubated with purified PH1 or PH2 (5 μg/mL) for an additional 1 h. Following incubation, strips were washed with TBST and incubated with 6×-His Tag Monoclonal Antibody, HRP (1:900) for 1 h. Strips were then washed with TBST and developed using SuperSignal West Pico PLUS Chemiluminescent Substrate before imaging. ..

    Article Title: Localized phosphoinositide metabolism regulates STIM1/ORAI1 fast inactivation
    Article Snippet: .. 624 Lipid-protein interaction assay 625 Recombinant GST-tagged MTMR7 was incubated with Membrane Lipid Strips (P-6002, Echelon 626 Biosciences), which contain pre-dotted spots of 15 distinct membrane lipids. ..

    Article Title: Localized phosphoinositide metabolism regulates STIM1/ORAI1 fast inactivation
    Article Snippet: .. Recombinant GST-tagged MTMR7 was incubated with Membrane Lipid Strips (P-6002, Echelon Biosciences), which contain pre-dotted spots of 15 distinct membrane lipids. ..

    Purification:

    Article Title: Two Pleckstrin homology domains scaffold SNAP-47-containing SNARE complexes
    Article Snippet: .. Echelon Biosciences Membrane Lipid Strips were blocked for 1 h with TBST containing 3% fatty-acid-poor BSA before being incubated with purified PH1 or PH2 (5 μg/mL) for an additional 1 h. Following incubation, strips were washed with TBST and incubated with 6×-His Tag Monoclonal Antibody, HRP (1:900) for 1 h. Strips were then washed with TBST and developed using SuperSignal West Pico PLUS Chemiluminescent Substrate before imaging. ..

    Imaging:

    Article Title: Two Pleckstrin homology domains scaffold SNAP-47-containing SNARE complexes
    Article Snippet: .. Echelon Biosciences Membrane Lipid Strips were blocked for 1 h with TBST containing 3% fatty-acid-poor BSA before being incubated with purified PH1 or PH2 (5 μg/mL) for an additional 1 h. Following incubation, strips were washed with TBST and incubated with 6×-His Tag Monoclonal Antibody, HRP (1:900) for 1 h. Strips were then washed with TBST and developed using SuperSignal West Pico PLUS Chemiluminescent Substrate before imaging. ..

    Recombinant:

    Article Title: Subversion of plant endomembrane trafficking by a Plasmodiophora brassicae effector PbALE that recruits membrane components at the host-pathogen interface to aid in colonization.
    Article Snippet: .. Echelon lipid stripsTM were probed with recombinant DSPPbALE-GFP, where free GFP was used as a control. ..

    Article Title: Localized phosphoinositide metabolism regulates STIM1/ORAI1 fast inactivation
    Article Snippet: .. 624 Lipid-protein interaction assay 625 Recombinant GST-tagged MTMR7 was incubated with Membrane Lipid Strips (P-6002, Echelon 626 Biosciences), which contain pre-dotted spots of 15 distinct membrane lipids. ..

    Article Title: Localized phosphoinositide metabolism regulates STIM1/ORAI1 fast inactivation
    Article Snippet: .. Recombinant GST-tagged MTMR7 was incubated with Membrane Lipid Strips (P-6002, Echelon Biosciences), which contain pre-dotted spots of 15 distinct membrane lipids. ..

    Control:

    Article Title: Subversion of plant endomembrane trafficking by a Plasmodiophora brassicae effector PbALE that recruits membrane components at the host-pathogen interface to aid in colonization.
    Article Snippet: .. Echelon lipid stripsTM were probed with recombinant DSPPbALE-GFP, where free GFP was used as a control. ..



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