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mouse l308 quantitative microarray  (RayBiotech inc)


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    RayBiotech inc mouse l308 quantitative microarray
    Fig. 4 Tet inhibited NLRP3 inflammasome activation by canonical and non-canonical pathways in vivo. a Functional enrichment analysis with KEGG biological processes of the significant different inflammatory protein in serum identified by quantitative inflammation <t>microarray</t> from control mice and silicosis mice. The top 20 pathways with a P < 0.05 were shown with their names. b Functional enrichment analysis with KEGG biological processes of the significantly different inflammatory protein in serum identified by quantitative inflammation microarray from silicosis mice treated with or without Tet. The top 20 pathways with a P < 0.05 were shown with their names. c Functional enrichment analysis with KEGG biological processes was performed using KEGG database in Si, Si + Tet, and PBS groups of mice treated as Fig. 2a (n = 3 per group). d The Venn diagram of transcriptome and quantitative inflammation microarray analysis. The intersecting signaling pathways were listed in the table in Fig. 4e. f-h The mRNA levels of TLR4, MyD88, and Caspase-11 of murine lung tissue in four groups (PBS + Vehicle, PBS + Tet, Si + Vehicle, and Si + Tet groups) treated as Fig. 2a (n = 9 per group). i Western blots of TLR4, MyD88, Pro-Caspase-11 and Cleaved Caspase-11 of murine lung tissue treated as (f). j Statistical analysis of band densities from images in (i). The data are reported as the mean ± SEM. Significance for each figure: **P < 0.01, ***P < 0.001 and ns indicates not significant.
    Mouse L308 Quantitative Microarray, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/array+membranes/Mouse+L308+Array%2C+Glass+Slide/pm34417574-104-25-30
    Average 94 stars, based on 12 article reviews
    mouse l308 quantitative microarray - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "Tetrandrine alleviates silicosis by inhibiting canonical and non-canonical NLRP3 inflammasome activation in lung macrophages."

    Article Title: Tetrandrine alleviates silicosis by inhibiting canonical and non-canonical NLRP3 inflammasome activation in lung macrophages.

    Journal: Acta pharmacologica Sinica

    doi: 10.1038/s41401-021-00693-6

    Fig. 4 Tet inhibited NLRP3 inflammasome activation by canonical and non-canonical pathways in vivo. a Functional enrichment analysis with KEGG biological processes of the significant different inflammatory protein in serum identified by quantitative inflammation microarray from control mice and silicosis mice. The top 20 pathways with a P < 0.05 were shown with their names. b Functional enrichment analysis with KEGG biological processes of the significantly different inflammatory protein in serum identified by quantitative inflammation microarray from silicosis mice treated with or without Tet. The top 20 pathways with a P < 0.05 were shown with their names. c Functional enrichment analysis with KEGG biological processes was performed using KEGG database in Si, Si + Tet, and PBS groups of mice treated as Fig. 2a (n = 3 per group). d The Venn diagram of transcriptome and quantitative inflammation microarray analysis. The intersecting signaling pathways were listed in the table in Fig. 4e. f-h The mRNA levels of TLR4, MyD88, and Caspase-11 of murine lung tissue in four groups (PBS + Vehicle, PBS + Tet, Si + Vehicle, and Si + Tet groups) treated as Fig. 2a (n = 9 per group). i Western blots of TLR4, MyD88, Pro-Caspase-11 and Cleaved Caspase-11 of murine lung tissue treated as (f). j Statistical analysis of band densities from images in (i). The data are reported as the mean ± SEM. Significance for each figure: **P < 0.01, ***P < 0.001 and ns indicates not significant.
    Figure Legend Snippet: Fig. 4 Tet inhibited NLRP3 inflammasome activation by canonical and non-canonical pathways in vivo. a Functional enrichment analysis with KEGG biological processes of the significant different inflammatory protein in serum identified by quantitative inflammation microarray from control mice and silicosis mice. The top 20 pathways with a P < 0.05 were shown with their names. b Functional enrichment analysis with KEGG biological processes of the significantly different inflammatory protein in serum identified by quantitative inflammation microarray from silicosis mice treated with or without Tet. The top 20 pathways with a P < 0.05 were shown with their names. c Functional enrichment analysis with KEGG biological processes was performed using KEGG database in Si, Si + Tet, and PBS groups of mice treated as Fig. 2a (n = 3 per group). d The Venn diagram of transcriptome and quantitative inflammation microarray analysis. The intersecting signaling pathways were listed in the table in Fig. 4e. f-h The mRNA levels of TLR4, MyD88, and Caspase-11 of murine lung tissue in four groups (PBS + Vehicle, PBS + Tet, Si + Vehicle, and Si + Tet groups) treated as Fig. 2a (n = 9 per group). i Western blots of TLR4, MyD88, Pro-Caspase-11 and Cleaved Caspase-11 of murine lung tissue treated as (f). j Statistical analysis of band densities from images in (i). The data are reported as the mean ± SEM. Significance for each figure: **P < 0.01, ***P < 0.001 and ns indicates not significant.

    Techniques Used: Activation Assay, In Vivo, Functional Assay, Microarray, Control, Protein-Protein interactions, Western Blot

    Related Articles

    Clinical Proteomics:

    Article Title: Western diet-induced visceral adipose tissue inflammation promotes Alzheimer’s disease pathology via microglial activation in a mouse model
    Article Snippet: For antibody array analysis, whole blood was collected from mice into Eppendorf tubes coated with 20 units of heparin and centrifuged at 2,500 × g for 15 min. .. The inflammation-related cytokines in the obtained plasma were quantified using the Mouse L308 Array (RayBiotech, Norcross, GA, USA) following the manufacturer’s instructions. .. The concentration of the purified sample was determined using a bicinchoninic acid assay (BCA) protein assay kit (Pierce; Thermo Fisher Scientific) with Multi-Skan FC (Thermo Fisher Scientific), and purity was verified through UV spectrometry.

    Article Title: A hepatocyte-specific cytochrome c oxidase deficiency in mice leads to a lymphopenia owing to deficiencies in bone marrow progenitors
    Article Snippet: .. Dialyzed WT and Sco1 plasma (38 μL) was prepared for and incubated with the Mouse L1308 Array Glass Slide kit (RayBiotech, Cat# AAM-BLG-1-4), according to the manufacturer’s instructions. .. The fully processed glass slide was imaged using the LI-COR Odyssey system (Software Version: 2.1.12) and processed using LI-COR Image Studio.

    Article Title: Elevated activity of plasma dipeptidyl peptidase 4 upon stress can be targeted to reverse tumor immunosuppression.
    Article Snippet: Supernatant was collected by centrifugation at 4000 ×g for 5 min. DPP4 activity was freshly quantified using the DPPIV-GloTM Protease Assay kit (Promega, Madison, WI). .. The abundance of plasma DPP4 was analyzed with LSeries Mouse Antibody Array 308 Glass Slide Kit (AAM-BLG-1–4, RayBio®). .. Full blood counts were quantified with 50 μL fresh blood samples using IDEXX ProCyte Dx® Haematology Analyser.

    Ab Array:

    Article Title: Cold Atmospheric Plasma‐Activated Decellularized Extracellular Matrix Gel as a Tumor‐Infiltrating Immunoactivation Platform for Post‐Surgical Cancer Immunotherapy
    Article Snippet: .. The supernatant was collected and analyzed using the Mouse Antibody Array 308 Glass Slide Kit (RayBiotech, Cat no. AAM‐BLG‐1‐4). ..

    Article Title: Elevated activity of plasma dipeptidyl peptidase 4 upon stress can be targeted to reverse tumor immunosuppression.
    Article Snippet: Supernatant was collected by centrifugation at 4000 ×g for 5 min. DPP4 activity was freshly quantified using the DPPIV-GloTM Protease Assay kit (Promega, Madison, WI). .. The abundance of plasma DPP4 was analyzed with LSeries Mouse Antibody Array 308 Glass Slide Kit (AAM-BLG-1–4, RayBio®). .. Full blood counts were quantified with 50 μL fresh blood samples using IDEXX ProCyte Dx® Haematology Analyser.

    other:

    Article Title: RAS/MEK/PI3K pathway inhibition augments response to CD40 agonism by targeting CD11b + Bregs thereby overcoming melanoma PD1-resistance
    Article Snippet: T-cell viability (Ghost Dye), activation (CD69) and IFNγ production were determined using intracellular staining by flow cytometry and analyzed using FlowJo 10.9.0 (RRID:SCR_008520).

    Article Title: A positive feedback loop between FGR and p65 sustains satellite glial cell activation and chronic neuropathic pain.
    Article Snippet: Briefly, an apparatus with two Plexiglas chambers (one compartment had a black floor and walls with an equally spaced stainless-steel stripe-like grid on the floor, and the other had a white floor and walls with a stainless steel grid on the floor) connected with an internal door (MED Associates Inc., MED-CPP2-3013-2AT) was used.

    Incubation:

    Article Title: A hepatocyte-specific cytochrome c oxidase deficiency in mice leads to a lymphopenia owing to deficiencies in bone marrow progenitors
    Article Snippet: .. Dialyzed WT and Sco1 plasma (38 μL) was prepared for and incubated with the Mouse L1308 Array Glass Slide kit (RayBiotech, Cat# AAM-BLG-1-4), according to the manufacturer’s instructions. .. The fully processed glass slide was imaged using the LI-COR Odyssey system (Software Version: 2.1.12) and processed using LI-COR Image Studio.



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