orca er cold ccd camera; detector array 1344 × 1024 px (Hamamatsu)
Structured Review

Orca Er Cold Ccd Camera; Detector Array 1344 × 1024 Px, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/array+detector/pmc02515298-54-37-43?v=Hamamatsu
Average 90 stars, based on 1 article reviews
Images
1) Product Images from "Extended Field Laser Confocal Microscopy (EFLCM): Combining automated Gigapixel image capture with in silico virtual microscopy"
Article Title: Extended Field Laser Confocal Microscopy (EFLCM): Combining automated Gigapixel image capture with in silico virtual microscopy
Journal: BMC Medical Imaging
doi: 10.1186/1471-2342-8-13
Figure Legend Snippet: Breaking the Gigapixel limit of a confluent confocal panorama image : A 2,8 Gigapixel size confocal mosaic, built up from 3000 (50 columns × 60 rows) individual confocal images (A), using the ImageLab/Virtual Microscope software. The internal structures of a mouse head are visualized with eosin fluorescence at 63X/NA1.25, individual images cover an area of 135 μm × 103 μm (B), captured at binning 1 (1344 × 1024 pixels) and 200 pixels overlap. After image processing in the ImageLab/Virtual Microscope software the final seamless confocal panorama show an area of 5,75 mm × 4,98 mm. The original resolution of each individual image is preserved in the final montage, allowing the user to zoom in on details at single cell level (C).
Techniques Used: Microscopy, Software, Fluorescence
Figure Legend Snippet: Example of application of the EFLCM method : To visualize thousands of cells growing in monolayer – for automatic and objective quantitation of epigenetic effects of the transformation associated viral nuclear antigen HHV8 LANA fused to green fluorescence protein in a transient transfection assay. The blue color is Hoecsht 33342 fluorescence showing DNA. The mosaic is built up from 100 images (10 columns × 10 rows) captured at 16X/NA O.5. Individual images covers an area of 522 μm × 398 μm. Images are captured at binning 1 (1344 × 1024 pixels) with 100 pixels overlap resulting in a final area of the seamless panorama, after image processing in the ImageLab/Virtual Microscope, of 4,82 mm × 3,58 mm.
Techniques Used: Quantitation Assay, Transformation Assay, Fluorescence, Transient Transfection Assay, Microscopy
